BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,839,854	C→A	40.1%	R455L (CGC→CTC)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,839,854	0	C	A	40.1%	303.1 / 241.1	270	R455L (CGC→CTC)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand): ref base C (65/95); new base A (47/63); total (112/158)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

CGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT  >  CP009273/1839706‑1840002

cgcgATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATcgc                                                                                                                                                      >  1:994094/1‑149 (MQ=255)

      cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCtt                                                                                                                                                <  1:112857/149‑1 (MQ=255)

      cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGCGCTCtt                                                                                                                                                >  2:3700931/1‑149 (MQ=255)

      cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCtt                                                                                                                                                >  2:4027999/1‑149 (MQ=255)

        cGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTtg                                                                                                                                              <  2:2749882/149‑1 (MQ=255)

          gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTtgct                                                                                                                                            <  1:3947598/149‑1 (MQ=255)

            cATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGCGCGCTTtgctgc                                                                                                                                          >  1:2649274/1‑149 (MQ=255)

             aTCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGcc                                                                                                                                         <  2:411544/149‑1 (MQ=255)

              gcGCCGAACGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACGTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCt                                                                                                                                        <  2:2706799/148‑1 (MQ=255)

                gCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTGATCGCGGGCGTTGGTGCCGaa                                                                                                                                      >  1:2819160/1‑149 (MQ=255)

                    aaaGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGc                                                                                                                                  >  2:3097070/1‑149 (MQ=255)

                      aGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGt                                                                                                                                >  2:2189821/1‑149 (MQ=255)

                        cAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTgc                                                                                                                              <  2:3495321/149‑1 (MQ=255)

                         aGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTgcg                                                                                                                             >  2:3500056/1‑149 (MQ=255)

                           tgtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGcc                                                                                                                           <  2:2034569/149‑1 (MQ=255)

                               gCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGcc                                                                                                                       <  1:2152403/149‑1 (MQ=255)

                               gCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGcc                                                                                                                       <  2:2110899/149‑1 (MQ=255)

                                 aTCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTTCCGAACAGCGTGCGCCAGccag                                                                                                                     >  1:4105696/1‑147 (MQ=255)

                                  tCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCAAGCCTGc                                                                                                                    >  2:1896083/1‑149 (MQ=255)

                                   cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCt                                                                                                                   <  1:3882313/149‑1 (MQ=255)

                                     gTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTc                                                                                                                 <  2:1506171/149‑1 (MQ=255)

                                     gTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTc                                                                                                                 >  2:2469727/1‑149 (MQ=255)

                                     gTAAAATGGCGCGGCGGCTGGGTTTGCCGATCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCTCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTc                                                                                                                 <  1:845449/149‑1 (MQ=255)

                                      tAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCa                                                                                                                <  2:3831315/149‑1 (MQ=255)

                                      tAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCa                                                                                                                <  1:1971884/149‑1 (MQ=255)

                                        aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGc                                                                                                              <  2:769648/149‑1 (MQ=255)

                                        aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGc                                                                                                              <  2:57751/149‑1 (MQ=255)

                                        aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTCATAGAGCTCTTTGCTGCCTAACAGCGTGCGCCCCCCAGCTTCAGc                                                                                                              >  2:721197/1‑149 (MQ=255)

                                         aaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCa                                                                                                             >  2:1679763/1‑149 (MQ=255)

                                         aaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACACATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAAAGGCGCTCTTTTGTTCCGAACAACGGGCGCCCCCCCGCTTAAGCa                                                                                                             >  1:1255715/1‑149 (MQ=255)

                                          aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTACCTAACAGCGTGCGCCAGCCTGCTTCAGCaa                                                                                                            <  1:528814/149‑1 (MQ=255)

                                          aTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCaa                                                                                                            <  1:1697679/149‑1 (MQ=255)

                                                  gcggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCGAAAAGCGTGCGCCCGCCTGCTTCAGCAAGAAAAccc                                                                                                    >  2:1792360/1‑147 (MQ=255)

                                                     gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCt                                                                                                 >  1:3063011/1‑149 (MQ=255)

                                                     gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCGCGCTCTTTGCTGCCCAACAGCGTGCGCCCGCCTGCTTCCGCACCAAAACgcgcg                                                                                                 >  2:2853225/1‑148 (MQ=255)

                                                       tGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCttt                                                                                               <  1:4032205/149‑1 (MQ=255)

                                                        gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTa                                                                                              <  1:3137357/149‑1 (MQ=255)

                                                        gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTa                                                                                              >  2:4024701/1‑149 (MQ=255)

                                                        gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTc                                                                                                                 >  2:3674375/1‑130 (MQ=255)

                                                        gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTc                                                                                                                 <  1:3674375/130‑1 (MQ=255)

                                                        gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATAGCGCTCTTTTCTTCCAAAAAGCCTGCGCCAGCCAGCTTAAGCAAGAAAACGCGCTTTa                                                                                              >  1:1012844/1‑149 (MQ=255)

                                                          gTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGc                                                                                            <  2:2859053/149‑1 (MQ=255)

                                                          gTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGc                                                                                            <  2:2859030/149‑1 (MQ=255)

                                                             tGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGAc                                                                                         <  2:2013360/149‑1 (MQ=255)

                                                               ccGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACaa                                                                                       <  2:3660246/149‑1 (MQ=255)

                                                                  ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAtt                                                                                    <  1:601951/149‑1 (MQ=255)

                                                                  ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAtt                                                                                    <  1:370321/149‑1 (MQ=255)

                                                                  ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAtt                                                                                    <  1:1623131/149‑1 (MQ=255)

                                                                      aCCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTAATCGAGCTCATTTGTGCCTAAAACCCTGCGCCAGCCAGCGTCAGCAAAAAAACACCCCTTATAGACaaaaattcc                                                                                >  1:2937975/1‑143 (MQ=255)

                                                                       ccACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCt                                                                               >  1:1668363/1‑149 (MQ=255)

                                                                       ccACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCt                                                                               <  1:2299613/149‑1 (MQ=255)

                                                                         aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATCGAGCGCTTTTGTGGCATACAGCGTGCGCCCGCCGGCGTCAGCCAGAAAACACGCGTTTGCGACAAAATTTGCttt                                                                             >  1:3481387/1‑149 (MQ=255)

                                                                         aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTCATAGAGCTCTTTTGTGCCAAACAGCGTGCGCCCGCCAGCTTCAGCAAGAAAAAGCGCTTTATCGACAAATTTTCCttt                                                                             >  1:3736121/1‑149 (MQ=255)

                                                                            tCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTATCGACAAATTTGCCTTTGGc                                                                          >  2:1380837/1‑149 (MQ=255)

                                                                            tCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGc                                                                          <  1:1232488/149‑1 (MQ=255)

                                                                               ccTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAAt                                                                       <  2:1718356/149‑1 (MQ=255)

                                                                               ccTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCGTTAGCGACAAATTTGCCTTTTGCCAt                                                                       >  2:1153310/1‑149 (MQ=255)

                                                                               ccTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGGTGTTCATAGCGCGCGTTTGTGCCGAAAACCCTTCGCCACCCCGCGTCAGCCACAAAAAACCCCTTAGCGACAAAAATTACTTTGGCaaa                                                                       >  1:2431722/1‑148 (MQ=255)

                                                                                     tCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAg                                                                 <  2:3376139/149‑1 (MQ=255)

                                                                                       acacaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGtt                                                               <  2:964084/149‑1 (MQ=255)

                                                                                         acaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCg                                                             <  1:2769556/149‑1 (MQ=255)

                                                                                               aCTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAaca                                                       <  2:3195523/149‑1 (MQ=255)

                                                                                                 tCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacaca                                                     <  1:3911248/149‑1 (MQ=255)

                                                                                                 tCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACCAATTTGCCTTTGGCAATGTCCAGGTCGATAacaca                                                     >  2:909997/1‑149 (MQ=255)

                                                                                                 tCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacaca                                                     >  2:316837/1‑149 (MQ=255)

                                                                                                  cATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacacac                                                    <  1:3149726/149‑1 (MQ=255)

                                                                                                   aTCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACt                                                   >  2:1621260/1‑149 (MQ=255)

                                                                                                    tCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACtt                                                  <  1:2027297/149‑1 (MQ=255)

                                                                                                      gCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGc                                                <  2:2772467/149‑1 (MQ=255)

                                                                                                        cTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCGCGCTATTTTCTGCCTAACAGCGTTCGCCAGCCTGCTTAAGCAAGAAAACGCGCTTTATCGACAAATTTTGCTTTGGCAAATTACAGTACGATAAAACACATTCgg                                              >  1:3730155/1‑149 (MQ=255)

                                                                                                          ttCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGaa                                            <  1:184349/149‑1 (MQ=255)

                                                                                                             gCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTGGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTGGCGGAacac                                         <  2:790899/149‑1 (MQ=255)

                                                                                                              ccaccaCAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTTCTGCCTAACAGCGTGCGCCAGCCTGCTTCATCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTAGATAACACACTTGCGGAACAcc                                        >  2:3002773/1‑149 (MQ=255)

                                                                                                                accacAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCGTTGGCAATGTCCAGTTCGATAACAAACTTGCGGAACACCGc                                      >  2:2246312/1‑149 (MQ=255)

                                                                                                                     aGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                                 <  2:2523400/149‑1 (MQ=255)

                                                                                                                       gCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCggg                               >  2:1730929/1‑149 (MQ=255)

                                                                                                                        cAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACGCCGCATCCGGGc                              <  2:3409912/149‑1 (MQ=255)

                                                                                                                        cAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGc                              <  2:3260850/149‑1 (MQ=255)

                                                                                                                        cAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGc                              <  1:3829126/149‑1 (MQ=255)

                                                                                                                            ggCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGaa                          <  2:1998908/149‑1 (MQ=255)

                                                                                                                             gCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAAt                         <  2:474814/149‑1 (MQ=255)

                                                                                                                             gCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAACAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAAt                         <  2:3421450/149‑1 (MQ=255)

                                                                                                                                   cgtcgtTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATc                   >  2:1188041/1‑149 (MQ=255)

                                                                                                                                   cgtcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATc                   <  1:3664037/149‑1 (MQ=255)

                                                                                                                                   cgtcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATc                   <  1:3271093/149‑1 (MQ=255)

                                                                                                                                    gtcgtTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa                  <  1:3689162/149‑1 (MQ=255)

                                                                                                                                    gtcgtTTTCTTCATCCCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCAACAAAACGCGCATTAGCGACAAATTTGCCTTTGGCAATGTACAGTTCGATAACACACTTGCGGAACAACGCATCCGGGCAGAATTGCaaaa                  >  2:2329805/1‑146 (MQ=255)

                                                                                                                                       gtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGAt               <  2:2150121/149‑1 (MQ=255)

                                                                                                                                        ttttCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATa              >  1:2518679/1‑149 (MQ=255)

                                                                                                                                         tttCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATAc             <  2:2430924/149‑1 (MQ=255)

                                                                                                                                          ttcttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACt            >  1:105598/1‑149 (MQ=255)

                                                                                                                                          ttcttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACt            >  2:534809/1‑149 (MQ=255)

                                                                                                                                          ttcttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACt            <  1:1437654/149‑1 (MQ=255)

                                                                                                                                           tcttcATCGAGCTCGTTGCTGCCTAACCGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTg           <  1:721197/149‑1 (MQ=255)

                                                                                                                                            cttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGa          <  2:2601318/149‑1 (MQ=255)

                                                                                                                                             ttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc         >  1:1212138/1‑149 (MQ=255)

                                                                                                                                              tcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg        >  2:73721/1‑149 (MQ=255)

                                                                                                                                               cATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACgg       <  1:2706227/149‑1 (MQ=255)

                                                                                                                                                 tCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGc     >  1:2425159/1‑149 (MQ=255)

                                                                                                                                                    cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAt  <  2:1255715/149‑1 (MQ=255)

CGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT  >  CP009273/1839706‑1840002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`