BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,840,377	C→A	5.4%	D281Y (GAT→TAT)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,840,377	0	C	A	5.4%	1239.4 / 25.8	328	D281Y (GAT→TAT)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand): ref base C (176/134); new base A (14/4); total (190/138)
Fisher's exact test for biased strand distribution p-value = 9.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 333 total aligned reads displayed.

CGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCA  >  CP009273/1840229‑1840525

cgcgGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAATGGCAGCGGCGCGGATTCTGATTCCCGTTTATc                                                                                                                                                      <  2:901641/149‑1 (MQ=255)

cgcgGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATc                                                                                                                                                      <  2:1554591/149‑1 (MQ=255)

 gcgGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCg                                                                                                                                                     >  1:1273771/1‑149 (MQ=255)

  cgGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGt                                                                                                                                                    <  2:1957274/149‑1 (MQ=255)

  cgGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGt                                                                                                                                                    <  2:2938080/149‑1 (MQ=255)

      tAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTtata                                                                                                                                                <  2:951707/149‑1 (MQ=255)

      tAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTtata                                                                                                                                                >  2:4014244/1‑149 (MQ=255)

      tAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTtata                                                                                                                                                >  2:1979335/1‑149 (MQ=255)

        aGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATAGTTATAGc                                                                                                                                              >  1:931208/1‑149 (MQ=255)

        aGTGATTAGATTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGc                                                                                                                                              <  1:2821150/149‑1 (MQ=255)

            aTTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGt                                                                                                                                          >  1:1770833/1‑149 (MQ=255)

              tAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGa                                                                                                                                        <  2:1185573/149‑1 (MQ=255)

                 cTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACaa                                                                                                                                     <  2:3296594/149‑1 (MQ=255)

                 cTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACaa                                                                                                                                     <  2:3170711/149‑1 (MQ=255)

                 cTTGTGCGTTTAGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACaa                                                                                                                                     <  2:106643/149‑1 (MQ=255)

                  ttGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAAt                                                                                                                                    <  1:3410012/149‑1 (MQ=255)

                     tgCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGc                                                                                                                                 <  1:1723261/149‑1 (MQ=255)

                     tgCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGc                                                                                                                                 <  2:1816905/149‑1 (MQ=255)

                     tgCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGc                                                                                                                                 <  1:3483573/149‑1 (MQ=255)

                             gTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGAc                                                                                                                         >  2:4037718/1‑149 (MQ=255)

                              tACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTATTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATAGTTATAGCTGGTGACAATAGCCGGTTGAcc                                                                                                                        <  2:3074153/149‑1 (MQ=255)

                               aCAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCa                                                                                                                       >  1:1485157/1‑149 (MQ=255)

                                cAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCAc                                                                                                                      >  1:1870302/1‑149 (MQ=255)

                                cAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCAc                                                                                                                      >  1:2640453/1‑149 (MQ=255)

                                cAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCAc                                                                                                                      <  2:3506280/149‑1 (MQ=255)

                                 aGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACt                                                                                                                     <  1:865714/149‑1 (MQ=255)

                                         gCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGt                                                                                                             >  1:242597/1‑149 (MQ=255)

                                         gCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGt                                                                                                             <  2:3449102/149‑1 (MQ=255)

                                          cAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGtt                                                                                                            <  1:4001862/149‑1 (MQ=255)

                                          cAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGtt                                                                                                            >  1:2202408/1‑149 (MQ=255)

                                            gATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTaa                                                                                                          <  2:1295998/149‑1 (MQ=255)

                                            gATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTaa                                                                                                          >  1:1731488/1‑149 (MQ=255)

                                             atatCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATAGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAAc                                                                                                         >  1:3590399/1‑149 (MQ=255)

                                              tatCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAAcc                                                                                                        >  2:3366462/1‑149 (MQ=255)

                                                tCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACcac                                                                                                      >  2:1083554/1‑149 (MQ=255)

                                                  aaGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACAt                                                                                                    <  1:3031317/149‑1 (MQ=255)

                                                  aaGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACAt                                                                                                    >  1:642216/1‑149 (MQ=255)

                                                   aGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATg                                                                                                   >  2:88556/1‑149 (MQ=255)

                                                         ttCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGc                                                                                             >  1:1108419/1‑149 (MQ=255)

                                                         ttCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAAAGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGc                                                                                             >  2:3240436/1‑149 (MQ=255)

                                                               gcACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTgg                                                                                       >  2:1712971/1‑149 (MQ=255)

                                                               gcACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTgg                                                                                       >  2:2456540/1‑149 (MQ=255)

                                                                cACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATAGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGa                                                                                      >  2:1830685/1‑149 (MQ=255)

                                                                    cAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGAt                                                                                  >  1:511667/1‑149 (MQ=255)

                                                                    cAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGAt                                                                                  >  2:844427/1‑149 (MQ=255)

                                                                    cAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGAt                                                                                  >  2:3474713/1‑149 (MQ=255)

                                                                    cAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTAGCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGAt                                                                                  >  1:2556296/1‑149 (MQ=255)

                                                                        ccAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTaa                                                                              >  1:3185011/1‑149 (MQ=255)

                                                                           aaaCGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACaa                                                                           >  2:2444867/1‑149 (MQ=255)

                                                                            aaCGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATAGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAg                                                                          >  2:372914/1‑149 (MQ=255)

                                                                             aCGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAgc                                                                         >  1:1560931/1‑149 (MQ=255)

                                                                             aCGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCACGTTTATCGTTATAGCTGGTGACAAAAGCCGGTTGACCACTAAAGCGGTTAAACACATCCACCGCCAGTGGACTATGTAACAAgc                                                                         >  1:192093/1‑149 (MQ=255)

                                                                                ttttGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGcc                                                                      >  1:576046/1‑149 (MQ=255)

                                                                                ttttGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGcc                                                                      >  2:2243163/1‑149 (MQ=255)

                                                                                ttttGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGcc                                                                      >  1:573160/1‑149 (MQ=255)

                                                                                  ttGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCct                                                                    <  2:2021089/149‑1 (MQ=255)

                                                                                   tGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCctt                                                                   >  1:372959/1‑149 (MQ=255)

                                                                                     ccGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCcttct                                                                 <  2:1598082/149‑1 (MQ=255)

                                                                                      cGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCcttctt                                                                <  1:2679155/149‑1 (MQ=255)

                                                                                        cTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCa                                                              >  2:3580281/1‑149 (MQ=255)

                                                                                        cTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCa                                                              >  2:1533921/1‑149 (MQ=255)

                                                                                         ttCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCAt                                                             >  2:2166627/1‑149 (MQ=255)

                                                                                           cAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATcc                                                           <  2:1747659/149‑1 (MQ=255)

                                                                                           cAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATcc                                                           >  1:710336/1‑149 (MQ=255)

                                                                                            aaTCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCt                                                          >  2:1460679/1‑149 (MQ=255)

                                                                                            aaTCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCt                                                          >  2:1332957/1‑149 (MQ=255)

                                                                                             aTCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTg                                                         >  1:1008800/1‑149 (MQ=255)

                                                                                              tCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTgg                                                        <  2:658154/149‑1 (MQ=255)

                                                                                                tGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCATATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTa                                                      >  1:1587008/1‑149 (MQ=255)

                                                                                                 gCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTAc                                                     >  1:954754/1‑149 (MQ=255)

                                                                                                    aCGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGt                                                  >  1:2255350/1‑149 (MQ=255)

                                                                                                    aCGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGt                                                  >  2:2843166/1‑149 (MQ=255)

                                                                                                        tGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTcac                                              >  1:1230798/1‑149 (MQ=255)

                                                                                                         gAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTcaca                                             <  2:1731472/149‑1 (MQ=255)

                                                                                                         gAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTcaca                                             >  2:2381989/1‑149 (MQ=255)

                                                                                                         gAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTcaca                                             >  2:2053259/1‑149 (MQ=255)

                                                                                                          aaaGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCacac                                            >  1:691502/1‑149 (MQ=255)

                                                                                                          aaaGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCacac                                            <  2:198346/149‑1 (MQ=255)

                                                                                                             gCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCt                                         >  1:1390263/1‑149 (MQ=255)

                                                                                                             gCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCt                                         >  1:735522/1‑149 (MQ=255)

                                                                                                              cGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCtt                                        <  1:1560225/149‑1 (MQ=255)

                                                                                                               gAAAAAGGCAGCGGCGCGGATTCTGAGTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTc                                       <  1:2094095/149‑1 (MQ=255)

                                                                                                                aaaaaGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCg                                      >  2:3098148/1‑149 (MQ=255)

                                                                                                                 aaaaGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGc                                     <  1:3832505/149‑1 (MQ=255)

                                                                                                                  aaaGGCAGCGGCGCGGATTCTGATTCCCGTTTATAGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCt                                    >  1:3525733/1‑149 (MQ=255)

                                                                                                                     ggCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCgg                                 >  1:3502779/1‑149 (MQ=255)

                                                                                                                       cAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGtt                               <  2:1870302/149‑1 (MQ=255)

                                                                                                                            gcgcGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAg                          >  2:737691/1‑149 (MQ=255)

                                                                                                                             cgcgGATTCTGATTCCCGTTTCTCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTGAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGa                         <  2:2391626/149‑1 (MQ=255)

                                                                                                                             cgcgGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGa                         >  1:4043879/1‑149 (MQ=255)

                                                                                                                                   ttCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCgg                   <  2:410711/149‑1 (MQ=255)

                                                                                                                                        aTTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAAc              <  1:1833357/149‑1 (MQ=255)

                                                                                                                                        aTTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAAc              <  1:3240436/149‑1 (MQ=255)

                                                                                                                                           cccGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGc           >  1:3999882/1‑149 (MQ=255)

                                                                                                                                           cccGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGc           >  2:2530697/1‑149 (MQ=255)

                                                                                                                                            ccGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCt          >  1:984777/1‑149 (MQ=255)

                                                                                                                                                ttATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATc      <  1:292853/149‑1 (MQ=255)

                                                                                                                                                ttATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATc      <  2:2902863/149‑1 (MQ=255)

                                                                                                                                                  aTCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCgg    >  2:3900644/1‑149 (MQ=255)

                                                                                                                                                    cGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCa  <  1:1460679/149‑1 (MQ=255)

CGCGGATAAGTGATTAGCTTGTGCGTTTCGTACAGTTTCTGGCAGATATCAAGCACGTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCGCGGATTCTGATTCCCGTTTATCGTTATAGCTGGTGACAATAGCCGGTTGACCACTAATGCGGTTAACCACATGCTCCGCCAGTGGACGATGTAACAAGCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCA  >  CP009273/1840229‑1840525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`