Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,852,274 C→A 100% D273Y (GAT→TAT)  ydjJ ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,852,2740CA98.5% 655.9 / 0.5 202D273Y (GAT→TAT) ydjJputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (104/95);  total (104/98)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

ATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGT  >  CP009273/1852128‑1852418
                                                                                                                                                  |                                                                                                                                                
aTAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGTATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGc                                                                                                                                                <  1:3032553/149‑1 (MQ=255)
aTAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGc                                                                                                                                                <  1:110638/149‑1 (MQ=255)
 tAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGcc                                                                                                                                               >  2:2431056/1‑149 (MQ=255)
       tAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGtcc                                                                                                                                         >  1:350538/1‑147 (MQ=255)
         aaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAg                                                                                                                                       <  2:3053841/149‑1 (MQ=255)
         aaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAg                                                                                                                                       >  2:331654/1‑149 (MQ=255)
         aaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAg                                                                                                                                       <  2:1130432/149‑1 (MQ=255)
         aaTATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAg                                                                                                                                       <  2:1649863/149‑1 (MQ=255)
           tatGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTa                                                                                                                                     <  1:3927138/149‑1 (MQ=255)
           tatGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTa                                                                                                                                     <  1:461878/149‑1 (MQ=255)
                 tCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACcaaca                                                                                                                               >  2:2236395/1‑149 (MQ=255)
                  cACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACcaacaa                                                                                                                              >  1:3526783/1‑149 (MQ=255)
                   aCCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAAt                                                                                                                             >  1:922271/1‑149 (MQ=255)
                    ccATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATc                                                                                                                            <  1:3788238/149‑1 (MQ=255)
                     cATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCa                                                                                                                           <  2:2319823/149‑1 (MQ=255)
                      aTCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCAt                                                                                                                          >  1:3202529/1‑149 (MQ=255)
                        cGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATaa                                                                                                                        <  2:1552010/149‑1 (MQ=255)
                          aTTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAAtt                                                                                                                      >  1:317217/1‑149 (MQ=255)
                             tCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTa                                                                                                                   <  1:1111793/149‑1 (MQ=255)
                             tCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTa                                                                                                                   <  1:1112678/149‑1 (MQ=255)
                               acaTCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACATTACCAACAATCATAATTTTAcc                                                                                                                 >  2:48897/1‑149 (MQ=255)
                               acaTCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTAcc                                                                                                                 <  1:1515664/149‑1 (MQ=255)
                               acaTCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTAcc                                                                                                                 <  1:3428138/149‑1 (MQ=255)
                                caTCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTAccg                                                                                                                >  1:305878/1‑149 (MQ=255)
                                      aTCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCgcgc                                                                                                          >  2:186805/1‑149 (MQ=255)
                                        cGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAt                                                                                                        <  1:3794767/149‑1 (MQ=255)
                                        cGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAt                                                                                                        <  2:3130168/149‑1 (MQ=255)
                                         gCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAtt                                                                                                       >  1:2142910/1‑149 (MQ=255)
                                         gCCCTGAAGAAATAGATTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCAtt                                                                                                       >  2:3220420/1‑149 (MQ=255)
                                          cccTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTa                                                                                                      <  1:2160788/149‑1 (MQ=255)
                                           ccTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCTAATAGCCGGGTACCGTACCAACAATCATAATTTTACCGCCGCGCATTAc                                                                                                     <  1:3833125/149‑1 (MQ=255)
                                                agaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGAt                                                                                                >  2:1958535/1‑149 (MQ=255)
                                                 gaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATa                                                                                               <  1:594729/149‑1 (MQ=255)
                                                 gaaATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATa                                                                                               <  2:2128946/149‑1 (MQ=255)
                                                  aaaTAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATaa                                                                                              <  1:190702/149‑1 (MQ=255)
                                                    aTAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAgg                                                                                            >  1:1155128/1‑149 (MQ=255)
                                                     tAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGt                                                                                           <  1:983729/149‑1 (MQ=255)
                                                     tAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGt                                                                                           <  2:4545/149‑1 (MQ=255)
                                                         ttCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCt                                                                                       <  1:1597428/149‑1 (MQ=255)
                                                           cAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGt                                                                                     <  2:1417514/149‑1 (MQ=255)
                                                            aaTCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGtt                                                                                    >  1:1068664/1‑149 (MQ=255)
                                                            aaTCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGtt                                                                                    >  1:711909/1‑149 (MQ=255)
                                                              tCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTa                                                                                  <  1:3352252/149‑1 (MQ=255)
                                                              tCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTa                                                                                  >  1:684438/1‑149 (MQ=255)
                                                              tCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTa                                                                                  >  1:1839693/1‑149 (MQ=255)
                                                                  gACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACgg                                                                              >  2:3420553/1‑149 (MQ=255)
                                                                   aCCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGt                                                                             <  2:1065169/149‑1 (MQ=255)
                                                                    ccGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTg                                                                            <  1:2590267/149‑1 (MQ=255)
                                                                      ggATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGAc                                                                          <  2:4006253/149‑1 (MQ=255)
                                                                       gATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGAcc                                                                         <  2:3692572/149‑1 (MQ=255)
                                                                         tAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGc                                                                       >  2:2646590/1‑149 (MQ=255)
                                                                             gATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGaa                                                                   <  2:450182/149‑1 (MQ=255)
                                                                             gATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGaa                                                                   >  2:466265/1‑149 (MQ=255)
                                                                             gATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGaa                                                                   <  1:264905/149‑1 (MQ=255)
                                                                             gATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGaa                                                                   >  1:2694279/1‑149 (MQ=255)
                                                                              aTTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAAc                                                                  >  1:424751/1‑149 (MQ=255)
                                                                              aTTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAAc                                                                  <  1:3455019/149‑1 (MQ=255)
                                                                               ttGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAAcc                                                                 >  1:614385/1‑149 (MQ=255)
                                                                                  gCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGc                                                              >  2:188101/1‑149 (MQ=255)
                                                                                      aGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGtt                                                          <  1:2895947/149‑1 (MQ=255)
                                                                                       gCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGttt                                                         <  1:1012344/149‑1 (MQ=255)
                                                                                        cGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTATAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTc                                                        <  2:2759247/149‑1 (MQ=255)
                                                                                          gAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGa                                                      >  1:268779/1‑149 (MQ=255)
                                                                                            aTACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGaaa                                                    >  2:208573/1‑149 (MQ=255)
                                                                                            aTACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGaaa                                                    >  2:2687316/1‑149 (MQ=255)
                                                                                                 gTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAat                                               >  1:925048/1‑149 (MQ=255)
                                                                                                 gTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAat                                               >  1:2295653/1‑149 (MQ=255)
                                                                                                 gTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAat                                               >  1:2295607/1‑149 (MQ=255)
                                                                                                  tCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAata                                              >  2:1258274/1‑149 (MQ=255)
                                                                                                  tCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAata                                              >  1:172921/1‑149 (MQ=255)
                                                                                                    tGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATc                                            <  1:331654/149‑1 (MQ=255)
                                                                                                      gATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTg                                          >  2:2267243/1‑149 (MQ=255)
                                                                                                          gTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGcc                                      >  1:3558099/1‑149 (MQ=255)
                                                                                                           tGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGccc                                     <  2:884249/149‑1 (MQ=255)
                                                                                                           tGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGccc                                     >  1:3096632/1‑149 (MQ=255)
                                                                                                               ttCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGt                                 >  2:733868/1‑149 (MQ=255)
                                                                                                               ttCGCGATTGATTTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGt                                 >  2:4078719/1‑149 (MQ=255)
                                                                                                                    gattgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCg                            >  1:888564/1‑149 (MQ=255)
                                                                                                                    gattgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCg                            >  1:2341890/1‑149 (MQ=255)
                                                                                                                    gattgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCg                            >  1:219777/1‑149 (MQ=255)
                                                                                                                     attgattTTTAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTCTTTCGAAAACAATATCTGCGCCCATGTCTTCgg                           >  1:2410925/1‑149 (MQ=255)
                                                                                                                      ttgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGt                          <  2:3550646/149‑1 (MQ=255)
                                                                                                                      ttgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGt                          >  1:1781182/1‑149 (MQ=255)
                                                                                                                       tgattTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTa                         >  1:621018/1‑149 (MQ=255)
                                                                                                                         attTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTaaa                       <  2:1530760/149‑1 (MQ=255)
                                                                                                                         attTTGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTaaa                       <  2:1317743/149‑1 (MQ=255)
                                                                                                                           tttGAGGAAATTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAAtt                     <  2:1177678/149‑1 (MQ=255)
                                                                                                                                  aaaTTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACa              <  1:4073821/149‑1 (MQ=255)
                                                                                                                                   aaTTGATTGCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAg             >  2:669453/1‑149 (MQ=255)
                                                                                                                                    attgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAga            >  1:4111147/1‑148 (MQ=255)
                                                                                                                                    attgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGc            <  2:3776149/149‑1 (MQ=255)
                                                                                                                                     ttgatttCCGAATAGCCGTGTTCAGTACCAACAATCATAATTTTACCGCCGCGCTTTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAACACAATATCTGCGCCCATTTCTTCGGTAAATTGCTGACAGCg           <  2:3740546/149‑1 (MQ=255)
                                                                                                                                      tgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGt          <  2:57463/149‑1 (MQ=255)
                                                                                                                                      tgattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGt          <  2:684438/149‑1 (MQ=255)
                                                                                                                                       gattgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTg         >  1:3274116/1‑149 (MQ=255)
                                                                                                                                          tgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCaa      <  1:2473267/149‑1 (MQ=255)
                                                                                                                                          tgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCaa      <  1:2513820/149‑1 (MQ=255)
                                                                                                                                          tgCCGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCaa      <  2:87007/149‑1 (MQ=255)
                                                                                                                                             cGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAg   <  1:349768/149‑1 (MQ=255)
                                                                                                                                             cGAATAGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTAGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAg   <  1:1718079/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGGAATACCGTCTGGATAGTGACTTCGCGATTGATTTTGAGGAAATTGATTGCCGAATCGCCGGGTACAGTACCAACAATCATAATTTTACCGCCGCGCATTACCAGATAAGGTGCCTGTTTAACGGTGACCGCAGAACCCGCTGTTTCGAAAACAATATCTGCGCCCATGTCTTCGGTAAATTGCTGACAGCGTGCAATAGT  >  CP009273/1852128‑1852418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑