Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 185,393 A→T 100% F201I (TTC→ATC)  map ← methionine aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273185,3930AT99.0% 931.8 / ‑0.7 284F201I (TTC→ATC) mapmethionine aminopeptidase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (150/131);  total (151/133)
Fisher's exact test for biased strand distribution p-value = 6.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 284 total aligned reads displayed.

CGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATC  >  CP009273/185247‑185538
                                                                                                                                                  |                                                                                                                                                 
cGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCg                                                                                                                                                 <  1:1013921/149‑1 (MQ=255)
 gTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGt                                                                                                                                                >  2:123934/1‑149 (MQ=255)
  tCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACTGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTc                                                                                                                                               >  1:114155/1‑149 (MQ=255)
  tCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACTGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTc                                                                                                                                               >  1:2188403/1‑149 (MQ=255)
  tCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTc                                                                                                                                               >  1:2684911/1‑149 (MQ=255)
  tCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTc                                                                                                                                               <  1:1128411/149‑1 (MQ=255)
  tCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTGTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTc                                                                                                                                               >  1:636111/1‑149 (MQ=255)
   cAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCa                                                                                                                                              <  2:3340019/149‑1 (MQ=255)
    aGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCAt                                                                                                                                             >  2:3668007/1‑149 (MQ=255)
    aGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATAGTCAt                                                                                                                                             <  1:3847960/149‑1 (MQ=255)
           cGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGt                                                                                                                                      <  2:3172236/149‑1 (MQ=255)
           cGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGt                                                                                                                                      <  2:1643294/149‑1 (MQ=255)
            gCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGtt                                                                                                                                     <  2:2995741/149‑1 (MQ=255)
             cAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGttt                                                                                                                                    <  1:3200871/149‑1 (MQ=255)
             cAGCCGTTATCAGTCACCACAATAGTATGCTCATAGTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGttt                                                                                                                                    <  1:2107288/149‑1 (MQ=255)
              aGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTc                                                                                                                                   <  1:901045/149‑1 (MQ=255)
              aGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTc                                                                                                                                   <  2:22020/149‑1 (MQ=255)
                 cGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGt                                                                                                                                <  2:3512242/149‑1 (MQ=255)
                    tATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacg                                                                                                                             >  2:3047916/1‑149 (MQ=255)
                     aTCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacga                                                                                                                            >  1:1886887/1‑149 (MQ=255)
                      tCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                           <  2:2440193/149‑1 (MQ=255)
                      tCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                           >  2:3232164/1‑149 (MQ=255)
                      tCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                           >  1:3690331/1‑149 (MQ=255)
                        aGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGt                                                                                                                         <  1:3814772/149‑1 (MQ=255)
                         gTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGtt                                                                                                                        >  2:2294128/1‑149 (MQ=255)
                         gTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGtt                                                                                                                        >  1:3756589/1‑149 (MQ=255)
                           caccacAATCGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTgg                                                                                                                      <  2:3939380/149‑1 (MQ=255)
                           caccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTgg                                                                                                                      >  1:1936470/1‑149 (MQ=255)
                             ccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGtt                                                                                                                    >  1:2226643/1‑149 (MQ=255)
                                caATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCa                                                                                                                 >  2:889966/1‑149 (MQ=255)
                                    aGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACggg                                                                                                             <  2:2722428/149‑1 (MQ=255)
                                    aGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACggg                                                                                                             <  1:2268224/149‑1 (MQ=255)
                                     gTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGa                                                                                                            <  2:1595978/149‑1 (MQ=255)
                                     gTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGa                                                                                                            >  1:2856087/1‑149 (MQ=255)
                                        tGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTc                                                                                                         >  2:1836530/1‑149 (MQ=255)
                                         gCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCa                                                                                                        >  2:8847/1‑149 (MQ=255)
                                          cTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAt                                                                                                       <  2:1667161/149‑1 (MQ=255)
                                           tCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTGACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATa                                                                                                      <  2:2904678/149‑1 (MQ=255)
                                               atTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgc                                                                                                  >  2:4050763/1‑149 (MQ=255)
                                                ttGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgca                                                                                                 <  2:299281/149‑1 (MQ=255)
                                                ttGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgca                                                                                                 >  1:3148501/1‑149 (MQ=255)
                                                 tgtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcag                                                                                                <  2:1997144/149‑1 (MQ=255)
                                                       gACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTg                                                                                          >  1:417364/1‑149 (MQ=255)
                                                           aGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGt                                                                                      >  2:2691147/1‑149 (MQ=255)
                                                               gCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttctt                                                                                  >  2:2450442/1‑149 (MQ=255)
                                                                 gATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa                                                                                >  1:773238/1‑149 (MQ=255)
                                                                 gATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa                                                                                >  1:2494685/1‑149 (MQ=255)
                                                                  aTCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAt                                                                               >  2:2961576/1‑149 (MQ=255)
                                                                       tGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAg                                                                          <  1:841342/149‑1 (MQ=255)
                                                                        ggTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGc                                                                         >  2:324503/1‑149 (MQ=255)
                                                                         gTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGcc                                                                        >  2:1497340/1‑149 (MQ=255)
                                                                         gTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGcc                                                                        <  1:3284758/149‑1 (MQ=255)
                                                                         gTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGcc                                                                        >  1:2458357/1‑149 (MQ=255)
                                                                           ttttACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcgc                                                                      <  2:1086552/149‑1 (MQ=255)
                                                                           ttttACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcgc                                                                      >  2:755438/1‑149 (MQ=255)
                                                                           ttttACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcgc                                                                      <  1:3048158/149‑1 (MQ=255)
                                                                                      aGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCg                                                           >  2:1234813/1‑149 (MQ=255)
                                                                                      aGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCg                                                           >  2:3778003/1‑149 (MQ=255)
                                                                                      aGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCg                                                           <  1:2526535/149‑1 (MQ=255)
                                                                                        ccATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtg                                                         >  2:1178724/1‑149 (MQ=255)
                                                                                        ccATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTATTCATGGAAGCCGCGACCAATACCgtg                                                         <  2:282555/149‑1 (MQ=255)
                                                                                         cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtgt                                                        <  2:862165/149‑1 (MQ=255)
                                                                                               tCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGTAGCCGCGACCAATACCGTGTCCGCaa                                                  >  2:462941/1‑149 (MQ=255)
                                                                                               tCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCaa                                                  <  2:1446153/149‑1 (MQ=255)
                                                                                                cATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAat                                                 <  2:1954272/149‑1 (MQ=255)
                                                                                                   ggTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATAtt                                              <  2:3965264/149‑1 (MQ=255)
                                                                                                       cGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacg                                          <  1:3767672/149‑1 (MQ=255)
                                                                                                        ggATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacga                                         <  1:8845/149‑1 (MQ=255)
                                                                                                          aTCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaac                                       >  1:2371692/1‑149 (MQ=255)
                                                                                                           tctcTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacg                                      >  1:3749250/1‑149 (MQ=255)
                                                                                                            ctctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacga                                     >  2:3487050/1‑149 (MQ=255)
                                                                                                             tctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgac                                    >  1:1661176/1‑149 (MQ=255)
                                                                                                               ttttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACgg                                  >  1:1914623/1‑149 (MQ=255)
                                                                                                                tttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGga                                 <  2:1559317/149‑1 (MQ=255)
                                                                                                                 ttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGgag                                >  1:3696447/1‑149 (MQ=255)
                                                                                                                  tttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGgaga                               <  1:445892/149‑1 (MQ=255)
                                                                                                                  tttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGgaga                               >  1:352598/1‑149 (MQ=255)
                                                                                                                      cccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGcc                           >  2:710116/1‑149 (MQ=255)
                                                                                                                       ccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCt                          <  2:2371692/149‑1 (MQ=255)
                                                                                                                       ccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCt                          <  1:3393227/149‑1 (MQ=255)
                                                                                                                         gcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTc                        >  2:370915/1‑149 (MQ=255)
                                                                                                                           gTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTg                      >  1:3411197/1‑149 (MQ=255)
                                                                                                                           gTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTg                      <  2:3830620/149‑1 (MQ=255)
                                                                                                                            ttGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGc                     <  1:2701144/149‑1 (MQ=255)
                                                                                                                                  aTTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGAc               >  2:3879432/1‑149 (MQ=255)
                                                                                                                                  aTTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGAc               >  1:3213460/1‑149 (MQ=255)
                                                                                                                                  aTTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGAc               >  1:933863/1‑149 (MQ=255)
                                                                                                                                   ttGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCGTCGAcc              >  2:2134872/1‑148 (MQ=255)
                                                                                                                                   ttGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAGACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACa              >  1:2739738/1‑149 (MQ=255)
                                                                                                                                     ggCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACaaa            <  1:1238745/149‑1 (MQ=255)
                                                                                                                                      gCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAt           <  1:4050763/149‑1 (MQ=255)
                                                                                                                                      gCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAt           >  2:50260/1‑149 (MQ=255)
                                                                                                                                          gATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt       <  2:2560762/149‑1 (MQ=255)
                                                                                                                                          gATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt       <  2:1369104/149‑1 (MQ=255)
                                                                                                                                           aTGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTg      >  1:1436175/1‑149 (MQ=255)
                                                                                                                                             ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGaa    <  2:2835037/149‑1 (MQ=255)
                                                                                                                                             ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGaa    <  1:3900084/149‑1 (MQ=255)
                                                                                                                                             ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGaa    <  2:636083/149‑1 (MQ=255)
                                                                                                                                             ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGaa    <  1:1090653/149‑1 (MQ=255)
                                                                                                                                              gTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAAt   >  1:1761849/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATC  >  CP009273/185247‑185538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑