Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,874,924 T→A 100% V31V (GTA→GTT)  leuE ← leucine efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,874,9240TA99.2% 788.1 / ‑2.7 239V31V (GTA→GTT) leuEleucine efflux protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (92/145);  total (92/147)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

TACAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGC  >  CP009273/1874780‑1875067
                                                                                                                                                |                                                                                                                                               
 aCAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACaaa                                                                                                                                            >  2:1617984/1‑149 (MQ=255)
 aCAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACaaa                                                                                                                                            <  2:2183311/149‑1 (MQ=255)
   aaTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGaacaaaca                                                                                                                                          >  1:4108846/1‑149 (MQ=255)
   aaTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGaacaaaca                                                                                                                                          <  1:431828/149‑1 (MQ=255)
   aaTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGaacaaaca                                                                                                                                          <  1:2451596/149‑1 (MQ=255)
    aTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAg                                                                                                                                         >  1:3995485/1‑149 (MQ=255)
    aTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAg                                                                                                                                         >  1:2688890/1‑149 (MQ=255)
    aTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAg                                                                                                                                         >  1:1957119/1‑149 (MQ=255)
        tGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTa                                                                                                                                     <  2:1836454/149‑1 (MQ=255)
          aataatATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCNGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTNGAGAACAAACAGGGTAtt                                                                                                                                   <  2:4050818/149‑1 (MQ=255)
           ataataTCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTAttt                                                                                                                                  <  2:1277391/149‑1 (MQ=255)
             natatCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTgg                                                                                                                                >  1:1950459/2‑149 (MQ=255)
              atatCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGc                                                                                                                               >  1:449869/1‑149 (MQ=255)
               tatCGGGGTGGTCGTAATTAATGTCGCCACTCCAGCCCATGCCAGCAACATCAATACCGCATCGCCAATCAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCGATTTTTGAGAACAAACAGGGTATTTGGcc                                                                                                                              >  2:2796861/1‑149 (MQ=255)
                atCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGccc                                                                                                                             <  2:534213/149‑1 (MQ=255)
                atCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGccc                                                                                                                             >  1:4002902/1‑149 (MQ=255)
                atCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGccc                                                                                                                             >  1:3508205/1‑149 (MQ=255)
                atCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGccc                                                                                                                             <  1:2815788/149‑1 (MQ=255)
                 tCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCt                                                                                                                            <  1:2074568/149‑1 (MQ=255)
                 tCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCt                                                                                                                            <  2:842824/149‑1 (MQ=255)
                  cGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTg                                                                                                                           <  1:2846248/149‑1 (MQ=255)
                    gggTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAAAAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTATTGAGAACAAACAGGGTATTTGGCCCTGGc                                                                                                                         >  1:1649086/1‑149 (MQ=255)
                       tggtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCAcc                                                                                                                      <  1:1310973/149‑1 (MQ=255)
                       tggtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCAcc                                                                                                                      >  2:1093483/1‑149 (MQ=255)
                         gtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGATATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCaa                                                                                                                    <  1:3604434/149‑1 (MQ=255)
                            ttaattaaTGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAacac                                                                                                                 <  1:1228751/149‑1 (MQ=255)
                             taattaatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAAcaca                                                                                                                >  1:3076461/1‑149 (MQ=255)
                              aattaatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACaa                                                                                                               <  1:2385229/149‑1 (MQ=255)
                                  aatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaa                                                                                                           <  1:4050360/149‑1 (MQ=255)
                                   atGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                          >  1:693467/1‑149 (MQ=255)
                                   atGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                          >  1:311356/1‑149 (MQ=255)
                                    tGTCGCCACTCCAGCCCAAGCCAGAAAAATCAATACCGCAACGCAAATAAAAACACCGCAGGACACAAGATAAAAGCCTTTCATACCGCTACTGACGCTATTATTGAGAACAAACATGGTATTTGTCACTGGCACCAACaaaaaaaaaa                                                                                                         >  1:257530/1‑149 (MQ=255)
                                     gTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACACTAAAAAt                                                                                                        >  2:2147853/1‑149 (MQ=255)
                                     gTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAAt                                                                                                        >  1:2658908/1‑149 (MQ=255)
                                          cACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGccc                                                                                                   >  2:2401099/1‑149 (MQ=255)
                                              ccAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAAc                                                                                               >  2:733330/1‑149 (MQ=255)
                                              ccAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAAc                                                                                               <  1:2719528/149‑1 (MQ=255)
                                                 gCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAg                                                                                            >  1:2312217/1‑149 (MQ=255)
                                                   ccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGAt                                                                                          <  2:1889543/149‑1 (MQ=255)
                                                     aTGCCATAAACATCAATACCGCATCGCAAATAAATACTCCTCAGGCCGCAATATAAACTCCTTTCATACAGCTACTGACGCTATTTTTGATAACAAACTGGGTATTTGGCACTGGCACCAAAACAATAAAAATGGACCCAACCAGATAg                                                                                        <  2:3627229/149‑1 (MQ=255)
                                                     aTGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAg                                                                                        >  1:553311/1‑149 (MQ=255)
                                                         cAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTcc                                                                                    <  2:632402/149‑1 (MQ=255)
                                                          aGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCa                                                                                   >  1:2725249/1‑149 (MQ=255)
                                                          aGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCa                                                                                   >  1:524770/1‑149 (MQ=255)
                                                           gAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAg                                                                                  <  2:1799461/149‑1 (MQ=255)
                                                           gAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAg                                                                                  >  2:31911/1‑149 (MQ=255)
                                                           gAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAg                                                                                  >  1:2019757/1‑149 (MQ=255)
                                                              aCATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTaa                                                                               <  1:2177327/149‑1 (MQ=255)
                                                              aCATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTaa                                                                               <  1:100027/149‑1 (MQ=255)
                                                                   aaTACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAg                                                                          >  1:261970/1‑149 (MQ=255)
                                                                   aaTACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAg                                                                          <  1:2370957/149‑1 (MQ=255)
                                                                     tACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGTTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACAAGATAGGTCCAGTAATTCAGaa                                                                        >  1:3326296/1‑149 (MQ=255)
                                                                     tACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGaa                                                                        <  1:385974/149‑1 (MQ=255)
                                                                        cGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAAccc                                                                     <  2:1902577/149‑1 (MQ=255)
                                                                        cGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAAccc                                                                     <  2:4100271/149‑1 (MQ=255)
                                                                         gCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAAcccc                                                                    <  2:2441841/149‑1 (MQ=255)
                                                                         gCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAAcccc                                                                    <  2:3301402/149‑1 (MQ=255)
                                                                            tCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTa                                                                 <  1:1054902/149‑1 (MQ=255)
                                                                              gCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAtt                                                               <  1:2891546/149‑1 (MQ=255)
                                                                                cAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCa                                                             <  1:1454863/149‑1 (MQ=255)
                                                                                cAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCa                                                             <  1:1617934/149‑1 (MQ=255)
                                                                                  ataaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGc                                                           <  2:2728524/149‑1 (MQ=255)
                                                                                   taaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCg                                                          <  2:2332494/149‑1 (MQ=255)
                                                                                     aataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGaa                                                        <  2:3508205/149‑1 (MQ=255)
                                                                                     aataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGaa                                                        <  2:544662/149‑1 (MQ=255)
                                                                                     aataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGaa                                                        >  2:2075840/1‑149 (MQ=255)
                                                                                        acacCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAacac                                                     <  1:2624514/149‑1 (MQ=255)
                                                                                         cacCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAAcaca                                                    <  2:947409/149‑1 (MQ=255)
                                                                                         cacCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAAcaca                                                    <  1:2777248/149‑1 (MQ=255)
                                                                                            cGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTa                                                 <  2:3076461/149‑1 (MQ=255)
                                                                                                ggCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACct                                             >  2:2044190/1‑149 (MQ=255)
                                                                                                ggCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACct                                             <  1:898261/149‑1 (MQ=255)
                                                                                                ggCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACct                                             <  1:1155604/149‑1 (MQ=255)
                                                                                                  cctcAAGATAACCGCCTTTCATAAAGCTACTGACTCTATTTTTGAGAACAATCAGTGTATTTGGCCCTGGCACCAAAACAATAACAATGGCACCAACCAGATTGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACAGATTAACctct                                           <  2:1725366/146‑1 (MQ=255)
                                                                                                   cGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCtt                                          <  1:3908586/149‑1 (MQ=255)
                                                                                                   cGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCtt                                          <  1:2780220/149‑1 (MQ=255)
                                                                                                    gCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCttt                                         <  2:425949/149‑1 (MQ=255)
                                                                                                    gCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCAAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCttt                                         <  2:420290/149‑1 (MQ=255)
                                                                                                       aGATAACCTCCTTTCATACCGCTTCTGACTCTATTTTTGATAACAAACAGGGTATTTGGCCCTGTCACCAACACAATATAAATGGCCCCACACATATAGGTCCAGTAATTCAGAACCCCGTATTCTGCGAACACATTAACCTCTTTAAt                                      <  2:257530/149‑1 (MQ=255)
                                                                                                            aCCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCt                                 <  1:2834137/149‑1 (MQ=255)
                                                                                                              cGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCttt                               <  2:2636313/149‑1 (MQ=255)
                                                                                                              cGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCttt                               <  2:2635562/149‑1 (MQ=255)
                                                                                                               gCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTc                              >  2:1482179/1‑149 (MQ=255)
                                                                                                                 cTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGa                            <  2:1098366/149‑1 (MQ=255)
                                                                                                                  tttCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGAt                           <  2:29221/149‑1 (MQ=255)
                                                                                                                   ttCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATc                          <  2:2914000/149‑1 (MQ=255)
                                                                                                                    tCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCa                         <  2:88995/149‑1 (MQ=255)
                                                                                                                     cATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCAt                        <  1:3688815/149‑1 (MQ=255)
                                                                                                                       tACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgc                      <  1:3375044/149‑1 (MQ=255)
                                                                                                                            cTACTGACGCTATTTTTGAGAACAAACATGGTATTTGGCCCTTGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTTACCTCTTTAATTATCTTTCGATCATGCGCGAt                 <  2:500564/149‑1 (MQ=255)
                                                                                                                            cTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGAt                 <  1:1093485/149‑1 (MQ=255)
                                                                                                                              aCTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTa               <  1:3280001/149‑1 (MQ=255)
                                                                                                                                     cTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGa        >  2:722729/1‑149 (MQ=255)
                                                                                                                                     cTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGa        >  2:1564913/1‑149 (MQ=255)
                                                                                                                                      tATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGaa       >  1:463316/1‑149 (MQ=255)
                                                                                                                                       aTTTTTGAGAACACACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAat      <  1:2266943/149‑1 (MQ=255)
                                                                                                                                         ttttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAatat    <  1:555565/149‑1 (MQ=255)
                                                                                                                                           ttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGc  >  2:3153327/1‑149 (MQ=255)
                                                                                                                                           ttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGc  <  2:2473785/149‑1 (MQ=255)
                                                                                                                                           ttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGc  >  2:3770019/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TACAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGC  >  CP009273/1874780‑1875067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑