Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,885,611 G→T 100% R628S (CGT→AGT)  yoaA ← putative ATP‑dependent helicase, DinG family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,885,6110GT99.5% 762.6 / ‑4.1 236R628S (CGT→AGT) yoaAputative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (121/113);  total (121/115)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

GTCGTTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACGAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCCC  >  CP009273/1885465‑1885756
                                                                                                                                                  |                                                                                                                                                 
gtcgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTaa                                                                                                                                                 <  2:120106/149‑1 (MQ=255)
 tcgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAAc                                                                                                                                                <  1:1053875/149‑1 (MQ=255)
 tcgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAAc                                                                                                                                                >  2:3666199/1‑149 (MQ=255)
 tcgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAAc                                                                                                                                                >  2:3212434/1‑149 (MQ=255)
 tcgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAAc                                                                                                                                                <  2:2257617/149‑1 (MQ=255)
 tcgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAAc                                                                                                                                                <  2:155511/149‑1 (MQ=255)
    ttCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGc                                                                                                                                             <  1:2414588/149‑1 (MQ=255)
        acaGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACgg                                                                                                                                         <  2:2433711/149‑1 (MQ=255)
        acaGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACgg                                                                                                                                         >  1:900169/1‑149 (MQ=255)
           gggCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAACCTAACCGCCCGGGCa                                                                                                                                      >  2:2884335/1‑149 (MQ=255)
             gCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAAt                                                                                                                                    <  2:2534434/149‑1 (MQ=255)
               gACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGt                                                                                                                                  <  1:2676363/149‑1 (MQ=255)
                aCAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTc                                                                                                                                 <  1:3218418/149‑1 (MQ=255)
                 cAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCa                                                                                                                                >  2:3404849/1‑149 (MQ=255)
                 cAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCa                                                                                                                                >  2:2957498/1‑149 (MQ=255)
                  agagCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCAc                                                                                                                               >  2:1767642/1‑149 (MQ=255)
                   gagCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACg                                                                                                                              >  2:684571/1‑149 (MQ=255)
                    agCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACgg                                                                                                                             >  1:2130022/1‑149 (MQ=255)
                      cAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGt                                                                                                                           <  2:306064/149‑1 (MQ=255)
                      cAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGt                                                                                                                           <  2:1691029/149‑1 (MQ=255)
                           ctctGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTg                                                                                                                      >  1:781439/1‑149 (MQ=255)
                               gTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGgcgc                                                                                                                  >  2:1509728/1‑149 (MQ=255)
                                 cgcgGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCgcgg                                                                                                                <  1:2751539/149‑1 (MQ=255)
                                  gcgGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCgcggg                                                                                                               >  1:2470881/1‑149 (MQ=255)
                                  gcgGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCgcggg                                                                                                               <  1:3120813/149‑1 (MQ=255)
                                    ggTATCGATAGCCAGAAGTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCgcgggcg                                                                                                             <  2:260854/149‑1 (MQ=255)
                                       aTCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCa                                                                                                          <  2:11415/149‑1 (MQ=255)
                                        tCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAg                                                                                                         >  2:2095857/1‑149 (MQ=255)
                                        tCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAg                                                                                                         <  1:2435113/149‑1 (MQ=255)
                                          gATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGAc                                                                                                       >  1:1007324/1‑149 (MQ=255)
                                          gATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGAc                                                                                                       <  1:1360356/149‑1 (MQ=255)
                                             aGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTgg                                                                                                    >  1:2098215/1‑149 (MQ=255)
                                              gCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGc                                                                                                   <  2:2056159/149‑1 (MQ=255)
                                                cAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCga                                                                                                 <  2:1564565/149‑1 (MQ=255)
                                                  gAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCgaga                                                                                               >  2:3895519/1‑149 (MQ=255)
                                                   aaTTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGaa                                                                                              >  2:1932984/1‑149 (MQ=255)
                                                     ttCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAAc                                                                                            >  2:3957470/1‑149 (MQ=255)
                                                     ttCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAAc                                                                                            >  1:699960/1‑149 (MQ=255)
                                                      tCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACg                                                                                           <  1:3652791/149‑1 (MQ=255)
                                                          aTGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTcgc                                                                                       >  1:2608408/1‑149 (MQ=255)
                                                           tGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTcgcg                                                                                      >  1:995642/1‑149 (MQ=255)
                                                           tGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTcgcg                                                                                      >  1:2839998/1‑149 (MQ=255)
                                                                 ttCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTaa                                                                                <  2:3546639/149‑1 (MQ=255)
                                                                 ttCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTaa                                                                                >  1:3520158/1‑149 (MQ=255)
                                                                 ttCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTaa                                                                                <  1:2535922/149‑1 (MQ=255)
                                                                  tCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAg                                                                               >  1:2202990/1‑149 (MQ=255)
                                                                    gTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGa                                                                             <  1:4024181/149‑1 (MQ=255)
                                                                       cTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGc                                                                          >  1:1589952/1‑149 (MQ=255)
                                                                       cTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGc                                                                          >  1:3816076/1‑149 (MQ=255)
                                                                       cTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGc                                                                          <  2:624109/149‑1 (MQ=255)
                                                                        ttAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAAGTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCa                                                                         <  2:3134315/149‑1 (MQ=255)
                                                                           gATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCa                                                                      >  2:782254/1‑149 (MQ=255)
                                                                                aTAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGc                                                                 >  1:1279152/1‑149 (MQ=255)
                                                                                aTAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGc                                                                 >  2:2701895/1‑149 (MQ=255)
                                                                                 tAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGcc                                                                >  2:2281240/1‑149 (MQ=255)
                                                                                   aaaaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGa                                                              >  1:846142/1‑149 (MQ=255)
                                                                                     aaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGAtt                                                            >  2:3259475/1‑149 (MQ=255)
                                                                                     aaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGAtt                                                            <  1:2037556/149‑1 (MQ=255)
                                                                                      aGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTg                                                           >  1:3651274/1‑149 (MQ=255)
                                                                                      aGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTg                                                           <  1:3312007/149‑1 (MQ=255)
                                                                                       ggCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGt                                                          <  1:582950/149‑1 (MQ=255)
                                                                                        gcgcgcATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTc                                                         <  2:1902659/149‑1 (MQ=255)
                                                                                        gcgcgcATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTc                                                         <  1:3306111/149‑1 (MQ=255)
                                                                                          gcgcATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTcac                                                       <  2:822372/149‑1 (MQ=255)
                                                                                             catcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACaaa                                                    <  2:2857897/149‑1 (MQ=255)
                                                                                              atcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAAt                                                   <  1:321323/149‑1 (MQ=255)
                                                                                               tcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATc                                                  >  1:1638221/1‑149 (MQ=255)
                                                                                               tcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATc                                                  >  2:1462188/1‑149 (MQ=255)
                                                                                                   accatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCa                                              <  2:3691738/149‑1 (MQ=255)
                                                                                                   accatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCa                                              >  1:3592084/1‑149 (MQ=255)
                                                                                                   accatacTCCGTAACAAAGTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCa                                              <  1:1897592/149‑1 (MQ=255)
                                                                                                     catacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGc                                            >  1:224200/1‑149 (MQ=255)
                                                                                                       tacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCAc                                          <  2:490849/149‑1 (MQ=255)
                                                                                                        acTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACg                                         >  2:219590/1‑149 (MQ=255)
                                                                                                        acTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACg                                         >  1:3567718/1‑149 (MQ=255)
                                                                                                        acTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACg                                         >  1:921430/1‑149 (MQ=255)
                                                                                                         cTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGc                                        >  1:2149298/1‑149 (MQ=255)
                                                                                                          tCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGcc                                       >  2:950776/1‑149 (MQ=255)
                                                                                                           ccGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCa                                      >  2:3114619/1‑149 (MQ=255)
                                                                                                           ccGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCa                                      <  1:384663/149‑1 (MQ=255)
                                                                                                           ccGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCAAGCCa                                      <  1:1103407/149‑1 (MQ=255)
                                                                                                             gTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACg                                    >  2:2812937/1‑149 (MQ=255)
                                                                                                              tAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGa                                   >  1:3566900/1‑149 (MQ=255)
                                                                                                               aaCAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAt                                  >  1:3372327/1‑149 (MQ=255)
                                                                                                                  aaaTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgt                               <  1:3942768/149‑1 (MQ=255)
                                                                                                                  aaaGTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgt                               <  2:1804369/149‑1 (MQ=255)
                                                                                                                    aTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgtcg                             <  2:2713333/149‑1 (MQ=255)
                                                                                                                    aTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgtcg                             >  2:809829/1‑149 (MQ=255)
                                                                                                                     ttACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCgg                            <  2:4042861/149‑1 (MQ=255)
                                                                                                                     ttACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCgg                            <  1:1767642/149‑1 (MQ=255)
                                                                                                                        ccTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGt                         >  1:284817/1‑149 (MQ=255)
                                                                                                                          tGGAGGATGGTATCGCAAGGAAACTAACCGAACGCGCAATGTCACGGTTGCTTGTAGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGAAAGTACGAATCACCAGCCCAGTGACACAAAAAACCAGCACGCAACGAacctcgcagaca                       >  2:3630089/1‑143 (MQ=255)
                                                                                                                              ggaTGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGaa                   <  1:1213207/149‑1 (MQ=255)
                                                                                                                                 tGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCa                >  2:194129/1‑149 (MQ=255)
                                                                                                                                  ggTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAg               >  2:1446562/1‑149 (MQ=255)
                                                                                                                                   gTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGt              <  2:780953/149‑1 (MQ=255)
                                                                                                                                        gaaaGGAAACTAACCGCACGGGATATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGccc         <  2:3816076/147‑1 (MQ=255)
                                                                                                                                        gCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGccc         >  2:4015282/1‑149 (MQ=255)
                                                                                                                                         cAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGAGTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCt        >  2:2948334/1‑149 (MQ=255)
                                                                                                                                               aaCTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTAccccc  <  2:1136532/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTCGTTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACGAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCCC  >  CP009273/1885465‑1885756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑