Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,899,496 C→T 5.3% L147F (CTT→TTT)  yobD → UPF0266 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,899,4960CT 5.3% 894.6 / 23.1 245L147F (CTT→TTT) yobDUPF0266 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (110/122);  new base T (4/9);  total (114/131)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 247 total aligned reads displayed.

AGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAA  >  CP009273/1899348‑1899642
                                                                                                                                                    |                                                                                                                                                  
aGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGc                                                                                                                                                    <  1:215600/149‑1 (MQ=255)
  gTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCtt                                                                                                                                                  >  1:3879771/1‑149 (MQ=255)
  gTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCtt                                                                                                                                                  <  1:3989455/149‑1 (MQ=255)
   ttttttCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTtc                                                                                                                                                 >  1:4085207/1‑149 (MQ=255)
   ttttttCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTtc                                                                                                                                                 <  1:600842/149‑1 (MQ=255)
    tttttCTACGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGTAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTtct                                                                                                                                                <  1:2455580/149‑1 (MQ=255)
      tttCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCg                                                                                                                                              >  2:3664954/1‑149 (MQ=255)
        tcttcGCCAATGTCTGGATTTAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGtt                                                                                                                                            <  1:2102659/149‑1 (MQ=255)
         cttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGttt                                                                                                                                           >  2:3494619/1‑149 (MQ=255)
            cGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCaa                                                                                                                                        >  1:154705/1‑149 (MQ=255)
              ccAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACt                                                                                                                                      >  1:1144234/1‑149 (MQ=255)
                aaTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCa                                                                                                                                    >  2:2102406/1‑149 (MQ=255)
                aaTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCa                                                                                                                                    >  2:2518507/1‑149 (MQ=255)
                 aTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGTTGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCaa                                                                                                                                   <  1:981562/149‑1 (MQ=255)
                 aTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCaa                                                                                                                                   >  1:2118676/1‑149 (MQ=255)
                   gTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATa                                                                                                                                 >  2:617382/1‑149 (MQ=255)
                   gTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATa                                                                                                                                 >  2:3947878/1‑149 (MQ=255)
                   gTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATa                                                                                                                                 >  2:1162122/1‑149 (MQ=255)
                      tGGATTGAATATAGCCGAATCTAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCTTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGt                                                                                                                              <  1:120339/149‑1 (MQ=255)
                      tGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGt                                                                                                                              >  2:2350377/1‑149 (MQ=255)
                      tGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGt                                                                                                                              >  1:911399/1‑149 (MQ=255)
                       ggATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGtt                                                                                                                             <  1:461752/149‑1 (MQ=255)
                       ggATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGtt                                                                                                                             >  2:2343484/1‑149 (MQ=255)
                             aaTATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAt                                                                                                                       <  2:1472636/149‑1 (MQ=255)
                              atatAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAtt                                                                                                                      >  2:546685/1‑149 (MQ=255)
                                   gCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGcc                                                                                                                 >  1:2004150/1‑149 (MQ=255)
                                       aaTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAg                                                                                                             >  2:3791873/1‑149 (MQ=255)
                                       aaTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAg                                                                                                             >  2:3630620/1‑149 (MQ=255)
                                       aaTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAg                                                                                                             >  1:3458927/1‑149 (MQ=255)
                                        aTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGc                                                                                                            >  1:3036927/1‑149 (MQ=255)
                                           aaaGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTAt                                                                                                         <  1:3153890/149‑1 (MQ=255)
                                           aaaGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTAt                                                                                                         >  1:3168161/1‑149 (MQ=255)
                                            aaGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTCATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATg                                                                                                        <  2:3339126/149‑1 (MQ=255)
                                             aGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGt                                                                                                       >  1:334555/1‑149 (MQ=255)
                                                gATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTa                                                                                                    <  1:3742896/149‑1 (MQ=255)
                                                     aCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTa                                                                                               >  1:2461624/1‑149 (MQ=255)
                                                        tGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTAttt                                                                                            >  1:1011314/1‑149 (MQ=255)
                                                              aaGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaata                                                                                      <  2:1443574/149‑1 (MQ=255)
                                                               aGATGGCGTTTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaataa                                                                                     <  1:165161/149‑1 (MQ=255)
                                                               aGATGGCGTGTTGGTGATGCAATTAGCACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaataa                                                                                     <  1:164096/149‑1 (MQ=255)
                                                               aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaataa                                                                                     <  2:2273359/149‑1 (MQ=255)
                                                               aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaataa                                                                                     <  2:731819/149‑1 (MQ=255)
                                                               aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaataa                                                                                     <  1:2682285/149‑1 (MQ=255)
                                                                 aTGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATc                                                                                   <  2:2610219/149‑1 (MQ=255)
                                                                  tGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCa                                                                                  >  2:653713/1‑149 (MQ=255)
                                                                  tGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCa                                                                                  >  2:449664/1‑149 (MQ=255)
                                                                      gtgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACa                                                                              <  2:208712/149‑1 (MQ=255)
                                                                      gtgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACa                                                                              >  2:2833128/1‑149 (MQ=255)
                                                                      gtgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACa                                                                              <  1:38982/149‑1 (MQ=255)
                                                                       tgtTGGTGATGAAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACAt                                                                             <  1:6522/149‑1 (MQ=255)
                                                                        gtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATa                                                                            <  1:3664954/149‑1 (MQ=255)
                                                                         ttGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                           <  1:622577/149‑1 (MQ=255)
                                                                         ttGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                           <  2:2501719/149‑1 (MQ=255)
                                                                         ttGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAATTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                           <  2:305719/149‑1 (MQ=255)
                                                                            gTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCtt                                                                        <  1:2735837/149‑1 (MQ=255)
                                                                             tGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTa                                                                       >  2:3992758/1‑149 (MQ=255)
                                                                             tGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTa                                                                       <  2:3867512/149‑1 (MQ=255)
                                                                             tGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTa                                                                       >  2:3166805/1‑149 (MQ=255)
                                                                               aTGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAgg                                                                     >  2:2384197/1‑149 (MQ=255)
                                                                                tGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGc                                                                    <  1:887253/149‑1 (MQ=255)
                                                                                     ttAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTatat                                                               >  2:2558015/1‑149 (MQ=255)
                                                                                       aGAACAGCGTCGGCTGTTAATCCGCGTTCTAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTa                                                             <  1:2537826/149‑1 (MQ=255)
                                                                                        gAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTAc                                                            >  2:3681016/1‑149 (MQ=255)
                                                                                        gAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTAc                                                            <  2:79278/149‑1 (MQ=255)
                                                                                        gAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTAc                                                            <  2:79711/149‑1 (MQ=255)
                                                                                                  ggCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCtt                                                  <  2:3879771/149‑1 (MQ=255)
                                                                                                   gCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTa                                                 >  2:1539648/1‑149 (MQ=255)
                                                                                                     tGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAtt                                               >  1:307211/1‑149 (MQ=255)
                                                                                                      gTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAttt                                              <  2:3411242/149‑1 (MQ=255)
                                                                                                          aTCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGtta                                          >  1:2555377/1‑149 (MQ=255)
                                                                                                           tCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttat                                         <  2:2012781/149‑1 (MQ=255)
                                                                                                            ccGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttatt                                        <  2:154705/149‑1 (MQ=255)
                                                                                                                 ttCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTatttattt                                   >  1:1697525/1‑149 (MQ=255)
                                                                                                                 ttCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTatttattt                                   >  1:321216/1‑149 (MQ=255)
                                                                                                                     aaaTATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAAc                               <  2:1856939/149‑1 (MQ=255)
                                                                                                                     aaaTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAAc                               >  1:173493/1‑149 (MQ=255)
                                                                                                                      aaTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACg                              <  1:2903135/149‑1 (MQ=255)
                                                                                                                      aaTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACg                              >  1:700481/1‑149 (MQ=255)
                                                                                                                        tatCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGtt                            <  1:1083927/149‑1 (MQ=255)
                                                                                                                        tatCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGtt                            <  2:1852822/149‑1 (MQ=255)
                                                                                                                          tCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTc                          <  2:2237774/149‑1 (MQ=255)
                                                                                                                           cgacgaTCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCa                         >  1:3601388/1‑149 (MQ=255)
                                                                                                                             acgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAtt                       >  1:371933/1‑149 (MQ=255)
                                                                                                                               gaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTTTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGa                     <  2:30168/149‑1 (MQ=255)
                                                                                                                               gaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGa                     <  2:20846/149‑1 (MQ=255)
                                                                                                                               gaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGa                     >  1:2394626/1‑149 (MQ=255)
                                                                                                                               gaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGa                     <  2:1314087/149‑1 (MQ=255)
                                                                                                                                  cTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatat                  <  2:1815442/149‑1 (MQ=255)
                                                                                                                                  cTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatat                  <  2:1816406/149‑1 (MQ=255)
                                                                                                                                  cTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatat                  >  1:1444901/1‑149 (MQ=255)
                                                                                                                                   tGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatata                 <  1:1338811/149‑1 (MQ=255)
                                                                                                                                   tGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatata                 >  1:556766/1‑149 (MQ=255)
                                                                                                                                      aaaaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATaa              >  1:227137/1‑149 (MQ=255)
                                                                                                                                       aaaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATaaa             >  1:2035719/1‑149 (MQ=255)
                                                                                                                                       aaaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATaaa             >  1:1782850/1‑149 (MQ=255)
                                                                                                                                        aaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAAt            >  2:279566/1‑149 (MQ=255)
                                                                                                                                        aaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAAt            >  1:1836565/1‑149 (MQ=255)
                                                                                                                                           aTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCa         >  1:3664305/1‑149 (MQ=255)
                                                                                                                                             ttATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCaaa       <  2:753740/149‑1 (MQ=255)
                                                                                                                                              tataAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTAACTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAAt      >  2:2939792/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAA  >  CP009273/1899348‑1899642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑