BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,922,164	C→A	30.2%	P311P (CCG→CCT)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,922,164	0	C	A	30.2%	432.5 / 169.0	225	P311P (CCG→CCT)	ptrB	protease II
Reads supporting (aligned to +/- strand): ref base C (88/69); new base A (29/39); total (117/108)
Fisher's exact test for biased strand distribution p-value = 8.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 229 total aligned reads displayed.

CATAGGCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAA  >  CP009273/1922016‑1922312

caTAGGCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGc                                                                                                                                                      <  2:867679/149‑1 (MQ=255)

   aGGCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGa                                                                                                                                                   >  1:1471133/1‑149 (MQ=255)

    ggCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGaa                                                                                                                                                  <  1:1405877/149‑1 (MQ=255)

      ccGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAAtt                                                                                                                                                <  1:2834957/149‑1 (MQ=255)

      ccGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAAtt                                                                                                                                                <  2:2755285/149‑1 (MQ=255)

       cGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTa                                                                                                                                               >  2:1410879/1‑149 (MQ=255)

        ggATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTaa                                                                                                                                              >  1:1048440/1‑149 (MQ=255)

        ggATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTaa                                                                                                                                              >  2:1700085/1‑149 (MQ=255)

         gATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAAc                                                                                                                                             >  1:3583376/1‑149 (MQ=255)

          atcatcAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAAct                                                                                                                                            >  2:3440112/1‑149 (MQ=255)

              tcaAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTc                                                                                                                                        >  1:1213268/1‑149 (MQ=255)

              tcaAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTc                                                                                                                                        >  1:1854609/1‑149 (MQ=255)

               caAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTcc                                                                                                                                       >  2:3642769/1‑149 (MQ=255)

                 aaGGCAATACCAATTACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCa                                                                                                                                     >  1:2413101/1‑149 (MQ=255)

                 aaGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCa                                                                                                                                     >  1:3312047/1‑149 (MQ=255)

                  aGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCAc                                                                                                                                    <  2:2650921/149‑1 (MQ=255)

                   ggCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACt                                                                                                                                   >  1:2160991/1‑149 (MQ=255)

                      aaTACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGtt                                                                                                                                <  2:4018451/149‑1 (MQ=255)

                      aaTACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGtt                                                                                                                                >  1:172577/1‑149 (MQ=255)

                      aaTACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGtt                                                                                                                                >  1:174148/1‑149 (MQ=255)

                       aTACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTg                                                                                                                               >  2:1234737/1‑149 (MQ=255)

                        tACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGc                                                                                                                              >  2:1318878/1‑149 (MQ=255)

                         aCCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCt                                                                                                                             >  2:1312832/1‑149 (MQ=255)

                           cAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCtca                                                                                                                           >  2:2007108/1‑149 (MQ=255)

                           cAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCtca                                                                                                                           >  2:3171845/1‑149 (MQ=255)

                             aTGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCtcatc                                                                                                                         <  1:170554/149‑1 (MQ=255)

                                aCTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACg                                                                                                                      <  1:1366065/149‑1 (MQ=255)

                                  ttCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCa                                                                                                                    >  1:1922684/1‑149 (MQ=255)

                                   tCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCAt                                                                                                                   <  1:3440112/149‑1 (MQ=255)

                                    cccGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATa                                                                                                                  <  2:297062/149‑1 (MQ=255)

                                    cccGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATa                                                                                                                  <  2:297164/149‑1 (MQ=255)

                                        ggTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACggg                                                                                                              <  1:2885801/149‑1 (MQ=255)

                                            ttGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACg                                                                                                          >  1:3501659/1‑149 (MQ=255)

                                               cGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGATCATCACGCATACGGGTACGGta                                                                                                       >  2:1529886/1‑149 (MQ=255)

                                                 gTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGtata                                                                                                     >  2:1025087/1‑149 (MQ=255)

                                                 gTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGtata                                                                                                     >  2:2292323/1‑149 (MQ=255)

                                                  ttAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATaa                                                                                                    <  1:3647641/149‑1 (MQ=255)

                                                   tAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAg                                                                                                   <  2:2708355/149‑1 (MQ=255)

                                                        tGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCaaa                                                                                              <  1:529353/149‑1 (MQ=255)

                                                             cAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCTACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGtttt                                                                                         <  1:2764875/149‑1 (MQ=255)

                                                              aaaCTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGttttt                                                                                        <  1:629935/149‑1 (MQ=255)

                                                                aCTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGc                                                                                      <  1:3028381/149‑1 (MQ=255)

                                                                aCTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGc                                                                                      >  1:209939/1‑149 (MQ=255)

                                                                 cTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGcc                                                                                     <  2:2445618/149‑1 (MQ=255)

                                                                   ggTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGt                                                                                   <  1:3023871/149‑1 (MQ=255)

                                                                    gTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTg                                                                                  >  2:1612103/1‑149 (MQ=255)

                                                                    gTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTg                                                                                  >  1:148855/1‑149 (MQ=255)

                                                                    gTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTg                                                                                  >  2:2962473/1‑149 (MQ=255)

                                                                     ttAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTgg                                                                                 >  2:4108346/1‑149 (MQ=255)

                                                                      tAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGc                                                                                <  2:1854609/149‑1 (MQ=255)

                                                                       aaCCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCg                                                                               >  1:1992000/1‑149 (MQ=255)

                                                                        aCCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCgg                                                                              >  2:2541167/1‑149 (MQ=255)

                                                                            gcgcTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTgg                                                                          >  2:1730868/1‑149 (MQ=255)

                                                                            gcgcTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTgg                                                                          <  2:2058259/149‑1 (MQ=255)

                                                                            gcgcTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGAGGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTgg                                                                          >  2:3090272/1‑149 (MQ=255)

                                                                               cTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAAc                                                                       >  1:2655877/1‑149 (MQ=255)

                                                                               cTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAAc                                                                       >  1:3128796/1‑149 (MQ=255)

                                                                                tGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACg                                                                      >  2:648864/1‑149 (MQ=255)

                                                                                 gACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGc                                                                     >  1:3047246/1‑149 (MQ=255)

                                                                                 gACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGc                                                                     >  1:713579/1‑149 (MQ=255)

                                                                                  aCGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCa                                                                    <  2:2160991/149‑1 (MQ=255)

                                                                                   cGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAg                                                                   <  1:3087083/149‑1 (MQ=255)

                                                                                   cGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAg                                                                   <  2:3723712/149‑1 (MQ=255)

                                                                                    gCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGa                                                                  <  2:1214613/149‑1 (MQ=255)

                                                                                                ccagccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGTAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAATTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGc                                                      <  1:2549590/149‑1 (MQ=255)

                                                                                                  agccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTg                                                    >  2:890467/1‑149 (MQ=255)

                                                                                                   gccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTGCCCACTGTTGCTCATCACGCAGACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTgg                                                   <  2:1472953/149‑1 (MQ=255)

                                                                                                      agTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAg                                                <  2:2393048/149‑1 (MQ=255)

                                                                                                      agTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAg                                                <  1:1617739/149‑1 (MQ=255)

                                                                                                       gTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGt                                               >  2:2362449/1‑149 (MQ=255)

                                                                                                        tCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCCCGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTg                                              <  2:2223656/149‑1 (MQ=255)

                                                                                                           gTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATc                                           >  1:1192610/1‑149 (MQ=255)

                                                                                                             aaaCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCaa                                         <  2:3287444/149‑1 (MQ=255)

                                                                                                              aaCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAg                                        >  2:210610/1‑149 (MQ=255)

                                                                                                               aCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAgg                                       >  2:508392/1‑149 (MQ=255)

                                                                                                                  gCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTg                                    >  1:3905954/1‑149 (MQ=255)

                                                                                                                  gCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTg                                    >  1:4044366/1‑149 (MQ=255)

                                                                                                                     tAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTAt                                 <  1:4045899/149‑1 (MQ=255)

                                                                                                                     tAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTAt                                 <  1:772305/149‑1 (MQ=255)

                                                                                                                        aCCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCg                              >  1:342065/1‑149 (MQ=255)

                                                                                                                         cccTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGt                             <  1:3642769/149‑1 (MQ=255)

                                                                                                                           cTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGa                           <  2:1474920/149‑1 (MQ=255)

                                                                                                                            ttCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGAt                          <  1:1318878/149‑1 (MQ=255)

                                                                                                                             tCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATc                         >  1:905269/1‑149 (MQ=255)

                                                                                                                             tCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATc                         <  2:2232503/149‑1 (MQ=255)

                                                                                                                             tCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATc                         <  2:156347/149‑1 (MQ=255)

                                                                                                                              ccAGCATGATGTGTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCt                        <  1:2277105/149‑1 (MQ=255)

                                                                                                                               cAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCtt                       <  1:1000549/149‑1 (MQ=255)

                                                                                                                                    tgatgTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcgg                  >  2:3919248/1‑149 (MQ=255)

                                                                                                                                    tgatgTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcgg                  <  2:1146894/149‑1 (MQ=255)

                                                                                                                                    tgatgTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcgg                  >  2:5669/1‑149 (MQ=255)

                                                                                                                                      atgTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcg                >  1:1245156/1‑149 (MQ=255)

                                                                                                                                           ttCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCa           >  2:3218816/1‑149 (MQ=255)

                                                                                                                                                gTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGaaaa      >  1:2938714/1‑149 (MQ=255)

                                                                                                                                                 tGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGaaaaa     >  1:3569636/1‑149 (MQ=255)

                                                                                                                                                  ggCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAaaac    >  1:2501040/1‑149 (MQ=255)

                                                                                                                                                   gCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATTCGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGGGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAaaaca   <  1:1410892/149‑1 (MQ=255)

                                                                                                                                                    cGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAaaacaa  <  2:710442/149‑1 (MQ=255)

                                                                                                                                                    cGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAaaacaa  <  2:2091201/149‑1 (MQ=255)

                                                                                                                                                    cGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAaaacaa  <  2:9213/149‑1 (MQ=255)

CATAGGCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAA  >  CP009273/1922016‑1922312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`