BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,922,899	G→T	6.1%	I66I (ATC→ATA)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,922,899	0	G	T	6.1%	843.3 / 18.1	227	I66I (ATC→ATA)	ptrB	protease II
Reads supporting (aligned to +/- strand): ref base G (99/114); new base T (6/8); total (105/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 242 total aligned reads displayed.

CGAGCAATGTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTAT  >  CP009273/1922751‑1923047

cGAGCAATGTTTCCCACTCATCCCACTCTTCCCTGAATGCCGATTGACGCTGGGAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCg                                                                                                                                                      <  2:1050543/149‑1 (MQ=255)

 gAGCAATGTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCga                                                                                                                                                     >  1:3558290/1‑149 (MQ=255)

  aGCAATGTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCgag                                                                                                                                                    >  1:3962044/1‑148 (MQ=255)

       tGTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGAttt                                                                                                                                               >  2:3578983/1‑149 (MQ=255)

        gTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTc                                                                                                                                              >  1:4103596/1‑149 (MQ=255)

           tCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCtt                                                                                                                                           >  1:1899305/1‑149 (MQ=255)

             ccACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGAGGATTTCCTTTa                                                                                                                                         >  2:3208323/1‑149 (MQ=255)

                cTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGa                                                                                                                                      >  1:3661853/1‑149 (MQ=255)

                cTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGa                                                                                                                                      >  1:2696953/1‑149 (MQ=255)

                cTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGa                                                                                                                                      <  2:1249057/149‑1 (MQ=255)

                 tCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGAt                                                                                                                                     >  2:771858/1‑149 (MQ=255)

                    tCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCg                                                                                                                                  >  2:661129/1‑149 (MQ=255)

                    tCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCg                                                                                                                                  >  2:2005534/1‑149 (MQ=255)

                      ccACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGAt                                                                                                                                <  2:2537321/149‑1 (MQ=255)

                         ctctTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCt                                                                                                                             <  1:3725163/149‑1 (MQ=255)

                         ctctTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCt                                                                                                                             <  1:1585850/149‑1 (MQ=255)

                         ctctTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCt                                                                                                                             <  2:2467051/149‑1 (MQ=255)

                         atctTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGAGCAGAAAATTCTAGTTGCGGAAAGCGGACGATGATTTCCTTTAAGTTTCGAACCt                                                                                                                             >  2:137424/2‑149 (MQ=255)

                           ctTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCTATGATTTCCTTTAAGATGCGATCCTGc                                                                                                                           <  2:3825250/149‑1 (MQ=255)

                           ctTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCTATGATTTCCTTTAAGATGCGATCCTGc                                                                                                                           <  2:3011465/149‑1 (MQ=255)

                            ttCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGTTTTCCTTTAAGATGCGATCCTGCa                                                                                                                          >  1:1624465/1‑149 (MQ=255)

                            ttCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGTTTTCCATTAAGATTCGAtacagcc                                                                                                                          >  1:1411193/1‑143 (MQ=255)

                            ttCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCa                                                                                                                          >  2:3466015/1‑149 (MQ=255)

                            ttCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCa                                                                                                                          <  1:1809612/149‑1 (MQ=255)

                                    aTGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCTATGATTTCCTTTAAGATGCGATCCTGCAAGGCttgt                                                                                                                  <  2:730195/149‑1 (MQ=255)

                                    aTGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCttgt                                                                                                                  <  2:3035066/149‑1 (MQ=255)

                                    aTGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCttgt                                                                                                                  <  1:334310/149‑1 (MQ=255)

                                             gACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGcc                                                                                                         >  1:1862611/1‑149 (MQ=255)

                                              aCGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCa                                                                                                        >  1:2075467/1‑149 (MQ=255)

                                              aCGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCa                                                                                                        <  1:3353121/149‑1 (MQ=255)

                                              aCGCTGGTAGATAGCATAGTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCa                                                                                                        <  2:3868131/149‑1 (MQ=255)

                                               cGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCAt                                                                                                       <  2:520969/149‑1 (MQ=255)

                                                    gTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCCTCAccc                                                                                                  >  2:3008357/1‑149 (MQ=255)

                                                    gTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCAccc                                                                                                  <  2:1543013/149‑1 (MQ=255)

                                                    gTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCAccc                                                                                                  <  2:16897/149‑1 (MQ=255)

                                                     tAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGTTTTGCGTTAAGATGCGCTCCAGCCAGGCTTGTTGTGAGGCCATCAcccc                                                                                                 >  1:3880108/1‑148 (MQ=255)

                                                       gATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGAt                                                                                               >  2:8959/1‑149 (MQ=255)

                                                         tAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGa                                                                                             <  1:2014719/149‑1 (MQ=255)

                                                          aGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGAc                                                                                            <  1:1024860/149‑1 (MQ=255)

                                                          aGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGAc                                                                                            <  1:1221600/149‑1 (MQ=255)

                                                            cATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCg                                                                                          <  2:1863183/149‑1 (MQ=255)

                                                              tatTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTa                                                                                        >  2:2018860/1‑149 (MQ=255)

                                                               atTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTaa                                                                                       <  2:1583720/149‑1 (MQ=255)

                                                                 tCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGAGGATTTCCTTTAAGATGCGATACTGCCAGGCTTGTTGTGAGGCCATCACCCCATGACCGTAACt                                                                                     >  1:2858885/1‑149 (MQ=255)

                                                                  cacaGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGGTTTCCTTTAAGATTCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTa                                                                                    >  1:2967771/1‑149 (MQ=255)

                                                                   acaGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTAt                                                                                   <  1:247727/149‑1 (MQ=255)

                                                                    caGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGCTGACCGTAACTAtt                                                                                  >  2:2179200/1‑149 (MQ=255)

                                                                     aGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTAttt                                                                                 <  2:3346010/149‑1 (MQ=255)

                                                                     aGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTAttt                                                                                 >  1:2673527/1‑149 (MQ=255)

                                                                      gCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTAtttt                                                                                <  1:1001852/149‑1 (MQ=255)

                                                                        cTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCt                                                                              <  2:542298/149‑1 (MQ=255)

                                                                         tGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCtt                                                                             >  2:2016859/1‑149 (MQ=255)

                                                                         tGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCtt                                                                             >  2:2095087/1‑149 (MQ=255)

                                                                         tGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCtt                                                                             <  2:1275600/149‑1 (MQ=255)

                                                                          ggTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCCAGGCTTGTTGTGAGGCCATCACCCCATGACCGTAACTATTttgttg                                                                            >  1:1584053/1‑149 (MQ=255)

                                                                          ggTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCttg                                                                            >  2:1952875/1‑149 (MQ=255)

                                                                           gTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCttgt                                                                           <  1:845468/149‑1 (MQ=255)

                                                                            ttCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCttgtt                                                                          <  1:2843866/149‑1 (MQ=255)

                                                                             tCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCttgttg                                                                         <  1:2428672/149‑1 (MQ=255)

                                                                                tAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAg                                                                      >  2:4042243/1‑149 (MQ=255)

                                                                                tAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAg                                                                      <  1:932492/149‑1 (MQ=255)

                                                                                  aaTATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGGTTTACTTTAAGATGCGATCCTGCAAGGCTTGTTGTGATGCCATCACCCCATGACCGTAACTATTTTCTTGTTGGAGGt                                                                    >  1:165177/1‑149 (MQ=255)

                                                                                  aaTATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGt                                                                    <  2:2920431/149‑1 (MQ=255)

                                                                                  aaTATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCGTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGt                                                                    >  2:1467042/1‑149 (MQ=255)

                                                                                     atGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGt                                                                 <  1:599009/149‑1 (MQ=255)

                                                                                       gCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTACTTTAAGATGCGATCCAGCAAGGCTTGTTGTGATGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTcc                                                               >  1:1944423/1‑149 (MQ=255)

                                                                                        ccGATAGCGGTAGCCATTTTTGATGTAGGGCACAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCa                                                              <  2:1935219/149‑1 (MQ=255)

                                                                                          gATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGTTTTACTTTAAGATGCGATCCTGCACGGCTTGTTGTGAGGGCCTCACCCCATGTCCGGAACTATTTTTTTGTTTGAGGGAGTGCAgg                                                            >  1:3453657/1‑149 (MQ=255)

                                                                                           aTAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGa                                                           <  2:1766385/149‑1 (MQ=255)

                                                                                                ggTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCt                                                      <  1:1396334/149‑1 (MQ=255)

                                                                                                  tAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTgg                                                    <  1:2439354/149‑1 (MQ=255)

                                                                                                       aTTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTATTGTTGCAGGTAGTCCAGGACTTCTGGCTgag                                               >  2:1155770/1‑149 (MQ=255)

                                                                                                        tttttGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGAGGATTTCCTTTAAGATTCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCCATGACCGTAACAATTTTCTTTTTGCAGGTAGTCCAGGACTTATGGCTgaga                                              >  1:4005028/1‑149 (MQ=255)

                                                                                                         ttttGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGaa                                             <  2:1397673/149‑1 (MQ=255)

                                                                                                          tttGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAAc                                            <  1:1023555/149‑1 (MQ=255)

                                                                                                              aTGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGt                                        >  2:2008736/1‑149 (MQ=255)

                                                                                                               tGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTa                                       <  2:871350/149‑1 (MQ=255)

                                                                                                               tGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTa                                       <  2:4103596/149‑1 (MQ=255)

                                                                                                               tGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTACTTTAAGATGCGATCCAGCAAGGCTTGTTGTGAGGCCATCACCCCATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTa                                       >  2:1847099/1‑149 (MQ=255)

                                                                                                                  aGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCGTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTCGTCCAGGACTTCTGGCTGAGAACGCGTATCg                                    >  2:272332/1‑149 (MQ=255)

                                                                                                                  aGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCAGCAAGGCTTGTTGTGAGGCCATCACCCCATGACCGTAACTATTTTCTTGTTGCAGGGAGTCCAGGACTTCTGGCTGAGAACGCGTATCg                                    >  2:1309567/1‑149 (MQ=255)

                                                                                                                    ggCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTc                                  <  1:202505/149‑1 (MQ=255)

                                                                                                                     gcgcAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTcc                                 <  1:1477524/149‑1 (MQ=255)

                                                                                                                       gcAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCGTTAAGATGCGATCCTGCCAGGCTTGTTTTGTGGCCAAAACCCGATGACCGTAACAATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCg                               >  1:2304575/1‑149 (MQ=255)

                                                                                                                       gcAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTACTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATAACCCGATGACCGTAACTAATTTCGTGTTGCAGGTAGTCCAGGACTTCTGGCGGAGAACGCGTATCGTcccc                               >  2:3152416/1‑148 (MQ=255)

                                                                                                                          gAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATACTGCAAGGCTTGTTGTGAGGCCATCACCCGATGAACGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTACCGCAg                            >  2:120823/1‑149 (MQ=255)

                                                                                                                          gAAACTTCTCGTTGCGGAATGCGGTCGAGGGTTTGCTTTAAGATGCGATCCGGCAAGGCGTGTTGTGTGGCCCACACCCCATGACCGTAACTATTTTCTTGTTGGAGGTAGGCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGccg                            >  1:3233330/1‑147 (MQ=255)

                                                                                                                           aaaCTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGc                           <  1:1190904/149‑1 (MQ=255)

                                                                                                                                tctcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCagta                      <  2:2314766/149‑1 (MQ=255)

                                                                                                                                 ctcGTTGCGGAATGCGGTCGATGTTTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCAACACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCagtag                     >  1:1351394/1‑149 (MQ=255)

                                                                                                                                 ctcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCagtag                     <  2:3661853/149‑1 (MQ=255)

                                                                                                                                  tcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCagtagt                    >  2:1666196/1‑149 (MQ=255)

                                                                                                                                    gTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTaa                  <  2:1213517/149‑1 (MQ=255)

                                                                                                                                           aaTGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGa           <  2:2640377/149‑1 (MQ=255)

                                                                                                                                            aTGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGAt          <  2:3558290/149‑1 (MQ=255)

                                                                                                                                            aTGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGAt          <  1:1999972/149‑1 (MQ=255)

                                                                                                                                              gCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATgc        <  1:1494063/149‑1 (MQ=255)

                                                                                                                                               cGGTCGAGGATTTCCTTTCAGCTGCGCTCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATgcg       <  1:2252539/149‑1 (MQ=255)

                                                                                                                                                ggTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATgcgc      >  2:1894991/1‑149 (MQ=255)

                                                                                                                                                    gatgatTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTAt  >  2:239898/1‑149 (MQ=255)

CGAGCAATGTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATATGCCGATAGCGGTAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTAT  >  CP009273/1922751‑1923047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`