Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,943,687 T→A 7.6% N365Y (AAC→TAC)  aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,943,6870TA 7.6% 738.2 / 38.7 236N365Y (AAC→TAC) aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (124/94);  new base A (7/11);  total (131/105)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 238 total aligned reads displayed.

CCACTTTCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCT  >  CP009273/1943540‑1943835
                                                                                                                                                   |                                                                                                                                                    
   cTTTCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATa                                                                                                                                                  <  1:516515/149‑1 (MQ=255)
   cTTTCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATa                                                                                                                                                  >  2:2578900/1‑149 (MQ=255)
    tttCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATAc                                                                                                                                                 >  1:1067151/1‑149 (MQ=255)
    tttCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATAc                                                                                                                                                 >  2:1086328/1‑149 (MQ=255)
    tttCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATAc                                                                                                                                                 >  1:3122177/1‑149 (MQ=255)
      tCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCt                                                                                                                                               <  1:3491953/149‑1 (MQ=255)
        aGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTc                                                                                                                                             >  1:3982074/1‑149 (MQ=255)
        aGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTc                                                                                                                                             <  2:1206412/149‑1 (MQ=255)
          gcgcAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCa                                                                                                                                           <  2:3649208/149‑1 (MQ=255)
               gTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCt                                                                                                                                      <  1:1582977/149‑1 (MQ=255)
               gTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCt                                                                                                                                      >  1:2769118/1‑149 (MQ=255)
                tGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCtt                                                                                                                                     <  1:4004644/149‑1 (MQ=255)
                 gCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCttt                                                                                                                                    <  2:3228868/149‑1 (MQ=255)
                 gCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCttt                                                                                                                                    >  1:739890/1‑149 (MQ=255)
                   aCCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGAATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTcg                                                                                                                                  >  2:1631159/1‑149 (MQ=255)
                    cccATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTcgc                                                                                                                                 <  1:3166689/149‑1 (MQ=255)
                    cccATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTcgc                                                                                                                                 >  1:2431941/1‑149 (MQ=255)
                    cccATCGCGTCGGAAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTcgc                                                                                                                                 <  1:3214202/149‑1 (MQ=255)
                      cATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTcgcgc                                                                                                                               >  2:4058188/1‑149 (MQ=255)
                      cATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCGTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTcgcgc                                                                                                                               >  1:340144/1‑149 (MQ=255)
                          gcgTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcgttc                                                                                                                           <  2:254126/149‑1 (MQ=255)
                          gcgTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcgttc                                                                                                                           >  2:3260246/1‑149 (MQ=255)
                           cgTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcgttcg                                                                                                                          <  1:644260/149‑1 (MQ=255)
                            ttCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcgttcgt                                                                                                                         <  2:2848131/148‑1 (MQ=255)
                             tCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcgttcgtt                                                                                                                        >  1:1517819/1‑149 (MQ=255)
                                gCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAAc                                                                                                                     >  2:2492167/1‑149 (MQ=255)
                                  aacaaTTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACtt                                                                                                                   <  2:3431376/149‑1 (MQ=255)
                                  aacaaTTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCAACTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTAGATACCTTCCAGACCTTTCGCGCGTTCGTTAACtt                                                                                                                   <  2:608944/149‑1 (MQ=255)
                                   acaaTTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACttt                                                                                                                  >  1:1145906/1‑149 (MQ=255)
                                       ttttCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATg                                                                                                              >  1:2286328/1‑149 (MQ=255)
                                         ttCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTa                                                                                                            <  1:1934112/149‑1 (MQ=255)
                                         ttCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTa                                                                                                            <  1:37236/149‑1 (MQ=255)
                                         ttCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTa                                                                                                            <  1:2116876/149‑1 (MQ=255)
                                         ttCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTa                                                                                                            >  2:2864178/1‑149 (MQ=255)
                                              gttgtCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCa                                                                                                       >  1:364399/1‑149 (MQ=255)
                                              gttgtCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCa                                                                                                       <  2:2247259/149‑1 (MQ=255)
                                              gttgtCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCa                                                                                                       >  1:1713847/1‑149 (MQ=255)
                                              gttgtCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCa                                                                                                       >  2:3027658/1‑149 (MQ=255)
                                                  tCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGAcc                                                                                                   >  1:2628508/1‑149 (MQ=255)
                                                    ggCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTAGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCtt                                                                                                 >  1:49890/1‑149 (MQ=255)
                                                     gCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCttt                                                                                                >  2:1263424/1‑149 (MQ=255)
                                                     gCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCttt                                                                                                <  1:2190696/149‑1 (MQ=255)
                                                     gCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCttt                                                                                                <  1:4089606/149‑1 (MQ=255)
                                                       aCCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTcg                                                                                              >  2:3536219/1‑149 (MQ=255)
                                                          gaagaaAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCgcgc                                                                                           >  2:3207411/1‑149 (MQ=255)
                                                            agaaAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCg                                                                                         >  1:1276393/1‑149 (MQ=255)
                                                                 aTCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGAt                                                                                    <  2:3766253/149‑1 (MQ=255)
                                                                 aTCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGAt                                                                                    >  2:3742653/1‑149 (MQ=255)
                                                                  tCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGAtt                                                                                   >  1:60184/1‑149 (MQ=255)
                                                                  tCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGAtt                                                                                   >  1:3815882/1‑149 (MQ=255)
                                                                    atatCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGAtttt                                                                                 <  1:1995982/149‑1 (MQ=255)
                                                                     tatCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTa                                                                                >  2:1371419/1‑149 (MQ=255)
                                                                       tCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAAc                                                                              <  1:184398/149‑1 (MQ=255)
                                                                            aTCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTAGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGt                                                                         >  2:61930/1‑149 (MQ=255)
                                                                             tCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGtt                                                                        >  1:6789/1‑149 (MQ=255)
                                                                             tCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGtt                                                                        <  2:378084/149‑1 (MQ=255)
                                                                                  cgcgGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGt                                                                   <  1:3654003/149‑1 (MQ=255)
                                                                                   gcgGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTa                                                                  >  2:465441/1‑149 (MQ=255)
                                                                                   gcgGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTa                                                                  >  2:4104279/1‑149 (MQ=255)
                                                                                       cAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTTTtcg                                                              <  1:876747/149‑1 (MQ=255)
                                                                                        aGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATtcgt                                                             <  1:3258162/149‑1 (MQ=255)
                                                                                         gTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATtcgtc                                                            <  2:1809163/149‑1 (MQ=255)
                                                                                               tCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTg                                                      >  2:1413412/1‑149 (MQ=255)
                                                                                                 cAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCt                                                    >  2:717729/1‑149 (MQ=255)
                                                                                                 cAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCt                                                    >  2:717826/1‑149 (MQ=255)
                                                                                                 cAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTAGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCt                                                    >  1:2062949/1‑149 (MQ=255)
                                                                                                  aGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCtt                                                   >  2:3921263/1‑149 (MQ=255)
                                                                                                   ggATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTa                                                  >  2:3753497/1‑149 (MQ=255)
                                                                                                    gATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTAc                                                 <  1:4042547/149‑1 (MQ=255)
                                                                                                     aTGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACg                                                >  2:1842123/1‑149 (MQ=255)
                                                                                                     aTGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACg                                                >  2:3951992/1‑149 (MQ=255)
                                                                                                        tCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTAGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGt                                             <  2:3429730/149‑1 (MQ=255)
                                                                                                        tCTTCGATGATTTCTGCATTAAGGAACGTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGt                                             <  1:1408698/149‑1 (MQ=255)
                                                                                                         cTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTc                                            <  1:2219599/149‑1 (MQ=255)
                                                                                                            cGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGc                                         <  2:3122177/149‑1 (MQ=255)
                                                                                                            cGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGc                                         <  2:1067151/149‑1 (MQ=255)
                                                                                                              atgatTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGa                                       >  1:3283290/1‑149 (MQ=255)
                                                                                                               tgatTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGAt                                      >  2:4018013/1‑149 (MQ=255)
                                                                                                                gatTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATg                                     <  2:2637904/149‑1 (MQ=255)
                                                                                                                 atTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATgc                                    <  2:2181056/149‑1 (MQ=255)
                                                                                                                  tttCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATgcg                                   >  2:1985570/1‑149 (MQ=255)
                                                                                                                  tttCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGAACTTTCGCGCGTTCGTTAACAATGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACTAAGATACCGTATTCGTCGATCTGCTTACGGGTCAGCGATgct                                   >  1:1684001/1‑148 (MQ=255)
                                                                                                                   ttCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATgcgc                                  <  2:1017400/149‑1 (MQ=255)
                                                                                                                       gCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCgccgcc                              >  2:1726784/1‑149 (MQ=255)
                                                                                                                         aTTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCGTACGGGTCAGCGATGCGCCGCCCg                            >  2:3363276/1‑149 (MQ=255)
                                                                                                                          ttAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCgg                           >  1:3578181/1‑149 (MQ=255)
                                                                                                                          ttAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCgg                           >  2:3183768/1‑149 (MQ=255)
                                                                                                                          ttAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCCTAGATTTTAACGAAGTTACCGTATTCCTCGATCAGCTTACGGGTCAGCGATGCGCCGCCCgg                           >  1:1577057/1‑149 (MQ=255)
                                                                                                                               gAACTTCGCTACCGGGCTGTAGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAAcgc                      <  2:613730/149‑1 (MQ=255)
                                                                                                                                aaCTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAAcgcg                     >  1:2154116/1‑149 (MQ=255)
                                                                                                                                 aCTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACgcgc                    >  1:2390504/1‑149 (MQ=255)
                                                                                                                                 aCTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACgcgc                    <  2:3404920/149‑1 (MQ=255)
                                                                                                                                   ttCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCag                  <  1:391679/149‑1 (MQ=255)
                                                                                                                                    tCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCaga                 >  2:2219648/1‑149 (MQ=255)
                                                                                                                                       cTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGcc              >  1:1915901/1‑149 (MQ=255)
                                                                                                                                       cTACCGGGCTGTAGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGcc              <  2:2840517/149‑1 (MQ=255)
                                                                                                                                            gggCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAc         >  1:1228701/1‑149 (MQ=255)
                                                                                                                                            gggCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAc         <  1:2685144/149‑1 (MQ=255)
                                                                                                                                                tGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGa     >  2:3621757/1‑149 (MQ=255)
                                                                                                                                                   tGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCt  >  2:2571631/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCACTTTCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCT  >  CP009273/1943540‑1943835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑