Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,962,923 C→A 100% M233I (ATG→ATT)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,962,9230CA99.0% 658.0 / ‑2.0 202M233I (ATG→ATT) cheRchemotaxis regulator, protein‑glutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (105/95);  total (105/97)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

GCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATG  >  CP009273/1962777‑1963067
                                                                                                                                                  |                                                                                                                                                
gcATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAAt                                                                                                                                                <  2:3678903/149‑1 (MQ=255)
  aTACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGa                                                                                                                                              <  2:1716195/149‑1 (MQ=255)
   tACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGAc                                                                                                                                             >  1:3418225/1‑149 (MQ=255)
     cacCGTCTGACCACGCAGCGTGAAGCGGCGCCCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGt                                                                                                                                           >  2:2320382/1‑149 (MQ=255)
        cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTAc                                                                                                                                        <  2:2624952/149‑1 (MQ=255)
         gTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACg                                                                                                                                       <  1:783816/149‑1 (MQ=255)
          tCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGa                                                                                                                                      >  2:1396687/1‑149 (MQ=255)
          tCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGa                                                                                                                                      <  1:174838/149‑1 (MQ=255)
           cTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGAc                                                                                                                                     <  2:2910187/149‑1 (MQ=255)
            tGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACa                                                                                                                                    <  2:713472/149‑1 (MQ=255)
              aCCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACaga                                                                                                                                  >  1:2369531/1‑149 (MQ=255)
                 aCGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                               >  2:824829/1‑149 (MQ=255)
                  cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGAt                                                                                                                              <  2:358796/149‑1 (MQ=255)
                  cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGAt                                                                                                                              <  2:359212/149‑1 (MQ=255)
                    cAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCg                                                                                                                            <  2:709536/149‑1 (MQ=255)
                      gCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCa                                                                                                                          >  1:1661885/1‑149 (MQ=255)
                      gCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCa                                                                                                                          <  1:1981082/149‑1 (MQ=255)
                      gCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCa                                                                                                                          >  1:245085/1‑149 (MQ=255)
                         tGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCa                                                                                                                       >  1:339774/1‑149 (MQ=255)
                          gAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCaa                                                                                                                      >  1:212493/1‑149 (MQ=255)
                           aaGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCaaa                                                                                                                     <  1:15089/149‑1 (MQ=255)
                               ggcgCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGc                                                                                                                 >  1:3231686/1‑149 (MQ=255)
                                 cgcTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGccc                                                                                                               <  2:3776428/149‑1 (MQ=255)
                                 cgcTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGccc                                                                                                               <  2:3492713/149‑1 (MQ=255)
                                 cgcTCAAGGTGGATAATGTTTTCAGTGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCTAAGTAGATAATGACGTTACGACAGATGATCGCATCAAACGGccc                                                                                                               <  2:13594/149‑1 (MQ=255)
                                   cTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCg                                                                                                             >  2:2882937/1‑149 (MQ=255)
                                    tCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCgg                                                                                                            >  1:2517289/1‑149 (MQ=255)
                                       aGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCAc                                                                                                         >  1:1423091/1‑149 (MQ=255)
                                        ggTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACg                                                                                                        >  1:3889249/1‑149 (MQ=255)
                                           ggCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGGCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgtg                                                                                                     <  1:114422/149‑1 (MQ=255)
                                            gCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgtgt                                                                                                    >  1:3702442/1‑149 (MQ=255)
                                            gCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgtgt                                                                                                    >  1:311774/1‑149 (MQ=255)
                                             cTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTa                                                                                                   >  2:1901011/1‑149 (MQ=255)
                                              tAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                  <  2:250722/149‑1 (MQ=255)
                                              tAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                  <  1:2871460/149‑1 (MQ=255)
                                              tAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                  >  1:3868490/1‑149 (MQ=255)
                                                aatttttCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGAAAAATCTCCTGCTGGTTAGTTTGATCGATGTATATAATCACGTTACGACAGAATATCGCAACAAACGGCCCCGGCACGGTGTACTg                                                                                                <  1:706121/146‑1 (MQ=255)
                                                aaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTg                                                                                                >  1:1532767/1‑149 (MQ=255)
                                                 aGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGt                                                                                               <  2:3872172/149‑1 (MQ=255)
                                                        agagTGACCCGCAAACAGCAATCCGTGGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACTTTACGACAGAAGATCGCAGCAAACGGCCCCGGCACGGTGTACTGTTTCGCCa                                                                                        <  1:3839073/149‑1 (MQ=255)
                                                         gagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAg                                                                                       <  2:3552129/149‑1 (MQ=255)
                                                         gagGGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAg                                                                                       <  2:701910/149‑1 (MQ=255)
                                                          agTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGt                                                                                      >  2:3404075/1‑149 (MQ=255)
                                                           gTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAACGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTa                                                                                     <  2:1540550/149‑1 (MQ=255)
                                                           gTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTa                                                                                     <  2:3890671/149‑1 (MQ=255)
                                                           gTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTGTGATCGAAGTCGATAATGACGTTACGACAGTAGATCGCATCTAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTa                                                                                     >  2:3333232/1‑149 (MQ=255)
                                                              aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAACGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAt                                                                                  <  1:62349/149‑1 (MQ=255)
                                                              aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAATTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAt                                                                                  <  1:2702185/149‑1 (MQ=255)
                                                              aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAt                                                                                  <  1:4045826/149‑1 (MQ=255)
                                                              aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAt                                                                                  <  2:1229113/149‑1 (MQ=255)
                                                                ccGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTc                                                                                >  2:1811481/1‑149 (MQ=255)
                                                                   cAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGc                                                                             >  2:614241/1‑149 (MQ=255)
                                                                   cAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGc                                                                             >  2:805748/1‑149 (MQ=255)
                                                                     aaCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                           >  1:2632109/1‑149 (MQ=255)
                                                                      aCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCggg                                                                          <  1:2073334/149‑1 (MQ=255)
                                                                            aTCCGTCGGGTTTAAGGAGCGGAACAACGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaaa                                                                    <  1:189888/149‑1 (MQ=255)
                                                                            aTCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaaa                                                                    <  2:3412351/149‑1 (MQ=255)
                                                                                 tCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAAc                                                               >  2:1918432/1‑149 (MQ=255)
                                                                                    ggTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATa                                                            >  2:2252210/1‑149 (MQ=255)
                                                                                     gTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAg                                                           >  1:969951/1‑149 (MQ=255)
                                                                                      tttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGt                                                          <  2:28462/149‑1 (MQ=255)
                                                                                       ttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGtt                                                         <  2:1115151/149‑1 (MQ=255)
                                                                                        tAAGGAGCGGACCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGACAAAATCAACATAGTTc                                                        >  2:2004858/1‑149 (MQ=255)
                                                                                         aaGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCg                                                       >  1:1334557/1‑149 (MQ=255)
                                                                                           ggAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc                                                     >  1:1126762/1‑149 (MQ=255)
                                                                                           ggAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc                                                     >  2:2816445/1‑149 (MQ=255)
                                                                                           ggAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc                                                     >  1:938294/1‑149 (MQ=255)
                                                                                                 gAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTcc                                               >  1:116072/1‑149 (MQ=255)
                                                                                                 gAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTcc                                               >  1:2977064/1‑149 (MQ=255)
                                                                                                        gcggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgca                                        >  2:3043671/1‑149 (MQ=255)
                                                                                                           gcgcAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgcacgc                                     <  2:612105/149‑1 (MQ=255)
                                                                                                               aaaaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAc                                 <  1:3048791/149‑1 (MQ=255)
                                                                                                                aaaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAcc                                <  1:1450857/149‑1 (MQ=255)
                                                                                                                aaaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAcc                                >  1:2045607/1‑149 (MQ=255)
                                                                                                                 aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCa                               <  1:804436/149‑1 (MQ=255)
                                                                                                                   tctcCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGc                             >  1:2292253/1‑149 (MQ=255)
                                                                                                                   tctcCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGc                             >  1:2294781/1‑149 (MQ=255)
                                                                                                                        tgctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTc                        <  2:2573085/149‑1 (MQ=255)
                                                                                                                            gggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGc                    >  2:859321/1‑149 (MQ=255)
                                                                                                                            gggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGc                    >  2:531890/1‑149 (MQ=255)
                                                                                                                             ggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCg                   <  2:2753772/149‑1 (MQ=255)
                                                                                                                                aGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGcc                >  1:364964/1‑149 (MQ=255)
                                                                                                                                 gTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGccc               <  1:2960674/149‑1 (MQ=255)
                                                                                                                                 gTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGccc               <  1:480368/149‑1 (MQ=255)
                                                                                                                                  tttGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGcccc              >  1:1021299/1‑149 (MQ=255)
                                                                                                                                  tttGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGcccc              >  1:2798765/1‑149 (MQ=255)
                                                                                                                                  tttGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGcccc              >  1:2348770/1‑149 (MQ=255)
                                                                                                                                     gATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTc           <  2:3070997/149‑1 (MQ=255)
                                                                                                                                     gATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTc           >  2:1286300/1‑149 (MQ=255)
                                                                                                                                      aTCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTcc          >  1:3645686/1‑149 (MQ=255)
                                                                                                                                      aTCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTcc          >  1:1597793/1‑149 (MQ=255)
                                                                                                                                       tCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTccc         <  2:3198629/149‑1 (MQ=255)
                                                                                                                                       tCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTccc         >  2:1683388/1‑149 (MQ=255)
                                                                                                                                        cGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCt        >  2:501252/1‑149 (MQ=255)
                                                                                                                                         gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTc       <  2:123039/149‑1 (MQ=255)
                                                                                                                                         gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTc       >  1:940570/1‑149 (MQ=255)
                                                                                                                                         gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTc       >  2:1427127/1‑149 (MQ=255)
                                                                                                                                         gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTc       >  2:2117745/1‑149 (MQ=255)
                                                                                                                                            gTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCa    >  1:2376121/1‑149 (MQ=255)
                                                                                                                                             tAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCAt   <  2:3231686/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATG  >  CP009273/1962777‑1963067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑