Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 199,229 G→T 100% E61D (GAG→GAT)  lpxA → UDP‑N‑acetylglucosamine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273199,2290GT97.2% 789.5 / 10.9 251E61D (GAG→GAT) lpxAUDP‑N‑acetylglucosamine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (5/2);  new base T (117/127);  total (122/129)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

ATTGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGA  >  CP009273/199083‑199374
                                                                                                                                                  |                                                                                                                                                 
aTTGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGatat                                                                                                                                                 >  1:1134510/1‑149 (MQ=255)
aTTGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGatat                                                                                                                                                 >  2:4078698/1‑149 (MQ=255)
aTTGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGatat                                                                                                                                                 >  1:2440737/1‑149 (MQ=255)
 ttGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATAtt                                                                                                                                                <  1:182913/149‑1 (MQ=255)
 ttGTGGAAGAGGGCGCGTCAATTGGCGAGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATAtt                                                                                                                                                <  2:4045688/149‑1 (MQ=255)
   gtgGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGGAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTa                                                                                                                                              <  1:3281882/149‑1 (MQ=255)
     ggAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATc                                                                                                                                            <  1:1994331/149‑1 (MQ=255)
      gAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCa                                                                                                                                           <  2:488989/149‑1 (MQ=255)
       aaGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAg                                                                                                                                          <  1:316853/149‑1 (MQ=255)
         gagGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGtt                                                                                                                                        <  1:209192/149‑1 (MQ=255)
          agGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTc                                                                                                                                       >  2:2145992/1‑149 (MQ=255)
               gcgTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTc                                                                                                                                  <  2:265632/149‑1 (MQ=255)
                cgTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTcc                                                                                                                                 >  1:563653/1‑149 (MQ=255)
                cgTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTcc                                                                                                                                 >  1:2345256/1‑149 (MQ=255)
                  tCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCAt                                                                                                                               <  2:2591925/149‑1 (MQ=255)
                  tCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCAt                                                                                                                               >  1:3167465/1‑149 (MQ=255)
                   cAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATc                                                                                                                              <  1:61871/149‑1 (MQ=255)
                   cAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATc                                                                                                                              >  1:3753314/1‑149 (MQ=255)
                      ttGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGc                                                                                                                           >  2:1306413/1‑149 (MQ=255)
                      ttGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGc                                                                                                                           >  2:3929771/1‑149 (MQ=255)
                       tGGCGCGAACGCACACATTGGACCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGAGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTTGCAGCGATAATGATATTTATCAGTTCGCCACCATCGGCg                                                                                                                          >  2:2541738/1‑149 (MQ=255)
                        ggCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGa                                                                                                                         <  1:1443427/149‑1 (MQ=255)
                         gcgcGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGaa                                                                                                                        <  2:51570/149‑1 (MQ=255)
                         gcgcGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTGGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGaa                                                                                                                        <  2:3753444/149‑1 (MQ=255)
                          cgcgAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAg                                                                                                                       <  2:3341323/149‑1 (MQ=255)
                           gcgAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGt                                                                                                                      <  1:3429822/149‑1 (MQ=255)
                             gAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGt                                                                                                                      <  2:3151460/147‑1 (MQ=255)
                             gAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTa                                                                                                                    >  1:1575159/1‑149 (MQ=255)
                             gAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTa                                                                                                                    >  2:3092627/1‑149 (MQ=255)
                                cGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAAcc                                                                                                                 >  1:2537695/1‑149 (MQ=255)
                                   acacaTTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGACGTTAACCAgg                                                                                                              >  2:1827443/1‑149 (MQ=255)
                                     acaTTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGAt                                                                                                            >  1:578171/1‑149 (MQ=255)
                                      caTTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATc                                                                                                           <  1:1639954/149‑1 (MQ=255)
                                      caTTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATc                                                                                                           <  1:1320674/149‑1 (MQ=255)
                                           gTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGaaa                                                                                                      <  1:3136495/149‑1 (MQ=255)
                                           gTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGaaa                                                                                                      <  2:3782097/149‑1 (MQ=255)
                                             ccTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAata                                                                                                    <  1:994501/149‑1 (MQ=255)
                                              cTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAatat                                                                                                   <  2:2162593/149‑1 (MQ=255)
                                              cTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAatat                                                                                                   >  1:744771/1‑149 (MQ=255)
                                                 tttGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCt                                                                                                <  2:2615197/149‑1 (MQ=255)
                                                  ttGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTg                                                                                               <  2:347319/149‑1 (MQ=255)
                                                   tGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTgg                                                                                              <  2:1941824/149‑1 (MQ=255)
                                                    gTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGc                                                                                             <  2:2873800/149‑1 (MQ=255)
                                                    gTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGc                                                                                             <  2:3817439/149‑1 (MQ=255)
                                                       tCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGaa                                                                                          >  2:4083113/1‑149 (MQ=255)
                                                       tCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGaa                                                                                          >  1:2322704/1‑149 (MQ=255)
                                                        cggtGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAAc                                                                                         <  2:709023/146‑1 (MQ=255)
                                                        cGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAAc                                                                                         <  1:1864425/149‑1 (MQ=255)
                                                            ggACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGAc                                                                                     <  1:1204756/149‑1 (MQ=255)
                                                             gACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGAcc                                                                                    >  2:1846099/1‑149 (MQ=255)
                                                                cccATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCgt                                                                                 >  1:3915728/1‑149 (MQ=255)
                                                                cccATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCgt                                                                                 >  2:2135347/1‑149 (MQ=255)
                                                                    tGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTgg                                                                             >  2:2204576/1‑149 (MQ=255)
                                                                       cGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGaaa                                                                          <  1:1754225/149‑1 (MQ=255)
                                                                        gAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAAt                                                                         <  2:2639710/149‑1 (MQ=255)
                                                                         aaaTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTATAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATc                                                                        <  1:1717151/149‑1 (MQ=255)
                                                                         aaaTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATc                                                                        <  1:3761247/149‑1 (MQ=255)
                                                                         aaaTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATc                                                                        <  2:552893/149‑1 (MQ=255)
                                                                          aaTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCg                                                                       <  1:1315440/149‑1 (MQ=255)
                                                                              ggTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGa                                                                   <  2:1350321/149‑1 (MQ=255)
                                                                                   gggTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTa                                                              <  2:3326695/149‑1 (MQ=255)
                                                                                   gggTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTa                                                              >  1:2229865/1‑149 (MQ=255)
                                                                                     gTACCGTACTGACATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAAGGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAAc                                                            <  1:2465070/149‑1 (MQ=255)
                                                                                     gTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAAc                                                            <  2:810569/149‑1 (MQ=255)
                                                                                     gTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAAc                                                            <  2:3984159/149‑1 (MQ=255)
                                                                                     gTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAAc                                                            <  1:2561253/149‑1 (MQ=255)
                                                                                        ccGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGc                                                         <  1:2606764/149‑1 (MQ=255)
                                                                                           tACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCAtt                                                      >  2:390628/1‑149 (MQ=255)
                                                                                             cTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTcg                                                    <  1:1783664/149‑1 (MQ=255)
                                                                                             cTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTcg                                                    >  2:1535369/1‑149 (MQ=255)
                                                                                              tGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTcgc                                                   <  1:996269/149‑1 (MQ=255)
                                                                                              tGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTcgc                                                   >  1:1387829/1‑149 (MQ=255)
                                                                                               gAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTcgcg                                                  >  1:2568827/1‑149 (MQ=255)
                                                                                                  aTCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGaaa                                               >  2:1085279/1‑149 (MQ=255)
                                                                                                  aTCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGaaa                                               <  1:430374/149‑1 (MQ=255)
                                                                                                   tctcACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAg                                              >  2:2696928/1‑149 (MQ=255)
                                                                                                   tctcACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAg                                              <  2:660022/149‑1 (MQ=255)
                                                                                                      cACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGt                                           >  1:3989155/1‑149 (MQ=255)
                                                                                                       aCGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTc                                          >  1:47789/1‑149 (MQ=255)
                                                                                                       aCGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTc                                          >  2:1612002/1‑149 (MQ=255)
                                                                                                       aCGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTc                                          >  2:3404754/1‑149 (MQ=255)
                                                                                                        cGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGGTCGTAACCGCATTCGCGAAAGCGTCa                                         <  2:2440742/149‑1 (MQ=255)
                                                                                                        cGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCa                                         <  1:3700405/149‑1 (MQ=255)
                                                                                                          ttGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCAcc                                       <  1:1186310/149‑1 (MQ=255)
                                                                                                          ttGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCAcc                                       >  1:2142227/1‑149 (MQ=255)
                                                                                                             tCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCAtt                                    >  2:1922443/1‑149 (MQ=255)
                                                                                                                  aaTGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCg                               >  1:3860389/1‑149 (MQ=255)
                                                                                                                    tGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTg                             <  1:245145/149‑1 (MQ=255)
                                                                                                                    tGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTg                             >  2:468217/1‑149 (MQ=255)
                                                                                                                      gTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGc                           >  2:3104671/1‑149 (MQ=255)
                                                                                                                             tAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTcc                    >  2:2994568/1‑149 (MQ=255)
                                                                                                                             tAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGGGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTcc                    >  2:3272243/1‑149 (MQ=255)
                                                                                                                               aaaTTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAg                  <  2:2845895/149‑1 (MQ=255)
                                                                                                                                  ttGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGc               <  2:2950967/149‑1 (MQ=255)
                                                                                                                                     gCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGt            <  1:1121979/149‑1 (MQ=255)
                                                                                                                                     gCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGt            >  1:2744396/1‑149 (MQ=255)
                                                                                                                                           aTAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTg      >  2:315548/1‑149 (MQ=255)
                                                                                                                                            tAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGa     >  1:3586355/1‑149 (MQ=255)
                                                                                                                                            tAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGa     >  2:2260944/1‑149 (MQ=255)
                                                                                                                                               tGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGa  >  1:283091/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATTGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGA  >  CP009273/199083‑199374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑