Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,986,289 T→A 100% N4Y (AAT→TAT)  pgsA ← phosphatidylglycerophosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,986,2890TA100.0% 862.4 / ‑6.7 259N4Y (AAT→TAT) pgsAphosphatidylglycerophosphate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (117/141);  minor base C (1/0);  total (118/141)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 267 total aligned reads displayed.

CAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCTGG  >  CP009273/1986143‑1986434
                                                                                                                                                  |                                                                                                                                                 
 aGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATaaaa                                                                                                                                                >  2:74332/1‑149 (MQ=255)
 aGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATaaaa                                                                                                                                                <  2:2559679/149‑1 (MQ=255)
  gAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAAt                                                                                                                                               <  1:320054/149‑1 (MQ=255)
    aaaCCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTg                                                                                                                                             >  1:1716070/1‑149 (MQ=255)
     aaCCATCGAACCACTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGAGTAAGCAACGAAGGGATATAAAATTGc                                                                                                                                            >  2:792520/1‑149 (MQ=255)
      aCCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCa                                                                                                                                           <  1:2227331/149‑1 (MQ=255)
       ccATCGATCAAGTCAGTCACCGCCGCGAAGCAGAAAATGAGCGCGGCGGAAATCGGCGACCAGGTGACAGGCAGATATAAGAACAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAt                                                                                                                                          <  2:4098427/149‑1 (MQ=255)
        cATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATa                                                                                                                                         >  2:1784689/1‑149 (MQ=255)
         aTCGAACCAGTCAGTCACCGCCGCGACGCAGAACATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGACTGGGATAAGGATGACACGGACCAGTGTAAGCAACGTAGGGATATAAAATTGCATaa                                                                                                                                        >  1:1822992/1‑149 (MQ=255)
         aTCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATaa                                                                                                                                        >  1:1823025/1‑149 (MQ=255)
          tCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAAt                                                                                                                                       <  1:560295/149‑1 (MQ=255)
               ccAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACgg                                                                                                                                  <  2:3344156/149‑1 (MQ=255)
                 agtcagtcACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGt                                                                                                                                >  1:3543562/1‑149 (MQ=255)
                  gtcagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTa                                                                                                                               <  1:955420/149‑1 (MQ=255)
                  gtcagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTa                                                                                                                               >  1:2177693/1‑149 (MQ=255)
                  gtcagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTa                                                                                                                               <  1:2863521/149‑1 (MQ=255)
                      gtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACTGAACAGTGTAAGCAACTTAGGGATATAAAATTGCATAATGACGGGTAACTa                                                                                                                           <  2:196437/149‑1 (MQ=255)
                      gtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTa                                                                                                                           <  2:2428334/149‑1 (MQ=255)
                      gtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTa                                                                                                                           <  2:3926938/149‑1 (MQ=255)
                       tcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTAt                                                                                                                          <  1:1413740/149‑1 (MQ=255)
                        caCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTTAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATc                                                                                                                         <  2:3748555/149‑1 (MQ=255)
                        caCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATc                                                                                                                         <  2:862407/149‑1 (MQ=255)
                         aCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCt                                                                                                                        <  1:2785076/149‑1 (MQ=255)
                          ccgccgCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCtg                                                                                                                       >  1:713310/1‑149 (MQ=255)
                            gccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCtgtt                                                                                                                     <  1:124085/149‑1 (MQ=255)
                              cgcgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCtgttgt                                                                                                                   <  1:1001692/149‑1 (MQ=255)
                               gcgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTc                                                                                                                  >  2:3358981/1‑149 (MQ=255)
                               gcgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTc                                                                                                                  >  2:1309259/1‑149 (MQ=255)
                                    gCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTaa                                                                                                             <  2:198430/149‑1 (MQ=255)
                                          aaTGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAc                                                                                                       <  2:6010/149‑1 (MQ=255)
                                                   ggcggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGtt                                                                                              <  1:1780322/149‑1 (MQ=255)
                                                      ggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGACTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCt                                                                                           <  1:631060/149‑1 (MQ=255)
                                                      ggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCt                                                                                           <  1:3035417/149‑1 (MQ=255)
                                                      ggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCt                                                                                           >  2:1777625/1‑149 (MQ=255)
                                                      ggcAAACGGCGACCAGGTGAAAGGCAGAAAAAAGACCAATACCAAGAAAGGGATAAGGAATACACCGAACAGTTTAAGCAACGGAGGGATATAAAATTGCATAATGACGCGTAACTAACTGTTCTCAGTAAGATTACCACTATGTTGCt                                                                                           >  2:3084064/1‑149 (MQ=255)
                                                        cAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTTACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTAc                                                                                         <  1:2002376/149‑1 (MQ=255)
                                                         aaaCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACa                                                                                        >  2:327403/1‑149 (MQ=255)
                                                          aaCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                       <  2:2987013/149‑1 (MQ=255)
                                                              gCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGAc                                                                                   <  2:3397652/149‑1 (MQ=255)
                                                              gCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGAc                                                                                   <  2:3397914/149‑1 (MQ=255)
                                                                   cAGGTGACAGGCAGATAAAAGACCAATACACAGCATCGGATCAGGACGACACGGAACACTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTTTCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCaa                                                                              <  2:4078105/149‑1 (MQ=255)
                                                                     ggTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATg                                                                            <  2:2848138/149‑1 (MQ=255)
                                                                     ggTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATg                                                                            >  1:3511586/1‑149 (MQ=255)
                                                                       tGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGtt                                                                          <  1:1447693/149‑1 (MQ=255)
                                                                       tGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGtt                                                                          <  1:3494766/149‑1 (MQ=255)
                                                                        gACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                         >  1:264302/1‑149 (MQ=255)
                                                                         aCAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTc                                                                        <  2:2726037/149‑1 (MQ=255)
                                                                           aGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCaa                                                                      <  2:3388596/149‑1 (MQ=255)
                                                                            ggCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAAc                                                                     >  2:580509/1‑149 (MQ=255)
                                                                              cAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGa                                                                   <  2:2064045/149‑1 (MQ=255)
                                                                               aGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAc                                                                  <  2:2331190/149‑1 (MQ=255)
                                                                                gATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTAAACGAcc                                                                 >  2:2873700/1‑149 (MQ=255)
                                                                                  tAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCag                                                               >  1:648850/1‑149 (MQ=255)
                                                                                  tAAAAGACCAATACAAAGAAGGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAAGGACGGGTAACTATCTGGGGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCag                                                               <  1:1678245/149‑1 (MQ=255)
                                                                                      aGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                           >  2:3592493/1‑149 (MQ=255)
                                                                                      aGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                           >  2:3163972/1‑149 (MQ=255)
                                                                                       gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGAt                                                          >  2:3136813/1‑149 (MQ=255)
                                                                                       gACAAATACAAAGAATGGGATAAGGATTACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAAATATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGAt                                                          >  2:2508928/1‑149 (MQ=255)
                                                                                         ccAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCt                                                        >  2:2958121/1‑149 (MQ=255)
                                                                                          cAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCtt                                                       <  2:769866/149‑1 (MQ=255)
                                                                                           aaTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCttt                                                      >  1:2552486/1‑149 (MQ=255)
                                                                                             tACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTc                                                    <  2:3851139/149‑1 (MQ=255)
                                                                                                 aaGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGcc                                                >  2:1341132/1‑149 (MQ=255)
                                                                                                 aaGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGca                                                >  2:3239320/1‑148 (MQ=255)
                                                                                                    aaTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGa                                             >  2:463333/1‑149 (MQ=255)
                                                                                                    aaTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGa                                             >  2:3024235/1‑149 (MQ=255)
                                                                                                     aTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGAc                                            >  1:1101087/1‑149 (MQ=255)
                                                                                                          aTAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATGGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGc                                       <  2:1330439/149‑1 (MQ=255)
                                                                                                           tAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCg                                      >  2:259658/1‑149 (MQ=255)
                                                                                                           tAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCg                                      >  1:82911/1‑149 (MQ=255)
                                                                                                            aaGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGa                                     >  1:3751934/1‑149 (MQ=255)
                                                                                                             aGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaa                                    >  2:3215818/1‑149 (MQ=255)
                                                                                                                 tGACACTGATCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAAATATCTTTTGTCAGTAAGATTACCCCTATTTTGCTACAGATACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAc                                <  1:1973486/149‑1 (MQ=255)
                                                                                                                 tGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAc                                >  1:3955625/1‑149 (MQ=255)
                                                                                                                 tGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAc                                <  1:3136813/149‑1 (MQ=255)
                                                                                                                   acacGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAccc                              <  2:2101440/149‑1 (MQ=255)
                                                                                                                        gAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAc                         >  1:1505718/1‑149 (MQ=255)
                                                                                                                         aaCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACt                        <  2:1412684/149‑1 (MQ=255)
                                                                                                                          aCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACtt                       >  2:507144/1‑149 (MQ=255)
                                                                                                                           cAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACttt                      <  2:1129623/149‑1 (MQ=255)
                                                                                                                           cAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACttt                      <  1:1309268/149‑1 (MQ=255)
                                                                                                                           cAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACttt                      <  1:2128010/149‑1 (MQ=255)
                                                                                                                           cAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACttt                      <  1:437812/149‑1 (MQ=255)
                                                                                                                           cAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACttt                      <  1:3358981/149‑1 (MQ=255)
                                                                                                                             gtgtAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTg                    >  2:1043959/1‑149 (MQ=255)
                                                                                                                             gtgtAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTg                    >  1:920364/1‑149 (MQ=255)
                                                                                                                              tgtAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGc                   >  2:1163666/1‑149 (MQ=255)
                                                                                                                                tAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCaa                 >  1:2253175/1‑149 (MQ=255)
                                                                                                                                 aaGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAt                <  2:2203422/149‑1 (MQ=255)
                                                                                                                                 aaGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAt                >  2:352537/1‑149 (MQ=255)
                                                                                                                                   gCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAttt              >  1:1063850/1‑149 (MQ=255)
                                                                                                                                   gCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAttt              <  2:3254403/149‑1 (MQ=255)
                                                                                                                                     aaCGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTcc            >  1:3855679/1‑149 (MQ=255)
                                                                                                                                         tAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGa        >  1:1236356/1‑149 (MQ=255)
                                                                                                                                          aGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGAc       >  2:1862703/1‑149 (MQ=255)
                                                                                                                                          aGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGAc       >  2:1689618/1‑149 (MQ=255)
                                                                                                                                          aGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGAc       >  2:951598/1‑149 (MQ=255)
                                                                                                                                            ggATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGc     >  2:395820/1‑149 (MQ=255)
                                                                                                                                            ggATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGc     >  1:2509942/1‑149 (MQ=255)
                                                                                                                                               tataAAATTGCATAATGACGGGTAACTATCTGTTGTCGGTAAGATTACCCCTATGTTGCTACAGAGACATCAATNTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCTgg  >  1:2082575/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCTGG  >  CP009273/1986143‑1986434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑