Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,993,530 C→A 100% E113* (GAG→TAG)  fliY ← cystine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,993,5300CA99.1% 731.8 / ‑3.4 221E113* (GAG→TAG) fliYcystine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (118/101);  total (118/103)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

TTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCG  >  CP009273/1993384‑1993675
                                                                                                                                                  |                                                                                                                                                 
ttCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaat                                                                                                                                                 <  2:1522671/149‑1 (MQ=255)
 tCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaatc                                                                                                                                                >  1:2874736/1‑149 (MQ=255)
 tCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaatc                                                                                                                                                <  1:2863828/149‑1 (MQ=255)
 tCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaatc                                                                                                                                                <  1:2540309/149‑1 (MQ=255)
 tCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGAGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaatc                                                                                                                                                >  1:3825455/1‑149 (MQ=255)
   aTAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAg                                                                                                                                              >  2:3931118/1‑149 (MQ=255)
    tAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGa                                                                                                                                             >  2:2684577/1‑149 (MQ=255)
      gTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGaaa                                                                                                                                           >  1:3877401/1‑149 (MQ=255)
      gTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGaaa                                                                                                                                           <  1:2853795/149‑1 (MQ=255)
      gTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGaaa                                                                                                                                           <  1:2757684/149‑1 (MQ=255)
       ttGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAAt                                                                                                                                          <  2:2523461/149‑1 (MQ=255)
         ggTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATgg                                                                                                                                        >  2:1845537/1‑149 (MQ=255)
           tGCCCAGACCGACCCCCACTTTTGTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTg                                                                                                                                      <  2:2675128/149‑1 (MQ=255)
            gCCCAGACCGACCCCCACTTTTTTGCCTTTCAGGTCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGa                                                                                                                                     <  2:219850/149‑1 (MQ=255)
            gCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGa                                                                                                                                     >  1:3230660/1‑149 (MQ=255)
            gCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGa                                                                                                                                     <  2:216664/149‑1 (MQ=255)
                 gaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                                >  2:682327/1‑149 (MQ=255)
                 gaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                                <  1:2706316/149‑1 (MQ=255)
                  accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAt                                                                                                                               <  1:2216726/149‑1 (MQ=255)
                   ccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAtt                                                                                                                              <  1:288610/149‑1 (MQ=255)
                         cccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                        >  2:223067/1‑149 (MQ=255)
                         cccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                        >  2:2969248/1‑149 (MQ=255)
                         cccACTTTTGTGCCTGTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                        <  2:3746034/149‑1 (MQ=255)
                          ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAAACGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcacc                                                                                                                       >  1:2467548/1‑149 (MQ=255)
                             cTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACcaca                                                                                                                    >  1:1030044/1‑149 (MQ=255)
                             cTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACcaca                                                                                                                    >  1:3086133/1‑149 (MQ=255)
                              ttttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACAt                                                                                                                   >  1:1845577/1‑149 (MQ=255)
                               tttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATc                                                                                                                  >  2:512053/1‑149 (MQ=255)
                                 tttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCaa                                                                                                                <  1:939559/149‑1 (MQ=255)
                                       tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                          <  1:1859745/149‑1 (MQ=255)
                                       tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                          >  1:3303682/1‑149 (MQ=255)
                                       tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                          >  2:1834203/1‑149 (MQ=255)
                                       tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                          >  1:3179492/1‑149 (MQ=255)
                                       tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                          <  2:416725/149‑1 (MQ=255)
                                       tttCAGATCATAGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                          <  2:3975749/149‑1 (MQ=255)
                                        ttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGttt                                                                                                         >  1:615869/1‑149 (MQ=255)
                                           aGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                      <  1:572681/149‑1 (MQ=255)
                                           aGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                      <  2:131681/149‑1 (MQ=255)
                                             atcatcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGt                                                                                                    <  1:3412690/149‑1 (MQ=255)
                                               catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc                                                                                                  >  2:328797/1‑149 (MQ=255)
                                                    gCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                             >  2:3656781/1‑149 (MQ=255)
                                                       gTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGc                                                                                          >  2:309216/1‑149 (MQ=255)
                                                       gTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGc                                                                                          >  1:2211455/1‑149 (MQ=255)
                                                       gTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATAACAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATTGTGACCTGATTAATCACCACATCAATAAGTTAAGAGTCCAGCGACGc                                                                                          >  1:2743726/1‑149 (MQ=255)
                                                          ttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAg                                                                                       >  2:3814541/1‑149 (MQ=255)
                                                            aaTGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCa                                                                                     >  1:2665092/1‑149 (MQ=255)
                                                            aaTGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCGAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCa                                                                                     <  2:3024480/149‑1 (MQ=255)
                                                              tGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATa                                                                                   >  1:3205158/1‑149 (MQ=255)
                                                               ggTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATAc                                                                                  >  1:2245074/1‑149 (MQ=255)
                                                                gTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATAcc                                                                                 <  2:641071/149‑1 (MQ=255)
                                                                   ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTc                                                                              >  1:1219771/1‑149 (MQ=255)
                                                                     ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTccc                                                                            >  2:781625/1‑149 (MQ=255)
                                                                        gTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAtt                                                                         <  2:3300597/149‑1 (MQ=255)
                                                                          tACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTg                                                                       <  2:2544233/149‑1 (MQ=255)
                                                                            ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGt                                                                     <  1:1727321/149‑1 (MQ=255)
                                                                             cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                    <  2:1068362/149‑1 (MQ=255)
                                                                             cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                    <  2:2278386/149‑1 (MQ=255)
                                                                             cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                    <  2:3692881/149‑1 (MQ=255)
                                                                             cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                    >  1:3707469/1‑149 (MQ=255)
                                                                             cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                    >  1:3707858/1‑149 (MQ=255)
                                                                              ttttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAATTGGTCg                                                                   >  2:96030/1‑149 (MQ=255)
                                                                                 tttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGtt                                                                <  2:1926286/149‑1 (MQ=255)
                                                                                     aCCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAg                                                            <  1:3161832/149‑1 (MQ=255)
                                                                                       cAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTg                                                          <  2:1618774/149‑1 (MQ=255)
                                                                                       cAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAAACGTATTTTTTCAACCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCTGTTTTAGTg                                                          >  1:1535103/1‑149 (MQ=255)
                                                                                        aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGa                                                         >  1:603128/1‑149 (MQ=255)
                                                                                        aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGa                                                         <  2:582790/149‑1 (MQ=255)
                                                                                         gcgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAc                                                        <  2:1459194/149‑1 (MQ=255)
                                                                                           gcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGc                                                      >  2:796298/1‑149 (MQ=255)
                                                                                                aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCaa                                                 <  1:3463738/149‑1 (MQ=255)
                                                                                                aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCaa                                                 >  1:1962141/1‑149 (MQ=255)
                                                                                                  tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACg                                               >  1:1625285/1‑149 (MQ=255)
                                                                                                    ccAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGcc                                             >  1:2374173/1‑149 (MQ=255)
                                                                                                       gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAg                                          >  1:3937224/1‑149 (MQ=255)
                                                                                                       gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAg                                          >  2:3354084/1‑149 (MQ=255)
                                                                                                          aTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATg                                       >  2:825049/1‑149 (MQ=255)
                                                                                                              tgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGtttt                                   <  2:3228136/149‑1 (MQ=255)
                                                                                                               gtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTg                                  <  2:2776413/149‑1 (MQ=255)
                                                                                                                tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGc                                 >  2:3459619/1‑149 (MQ=255)
                                                                                                                    gggTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGc                             <  2:2781102/149‑1 (MQ=255)
                                                                                                                    gggTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGc                             >  1:1665685/1‑149 (MQ=255)
                                                                                                                       ttGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGt                          <  1:1896319/149‑1 (MQ=255)
                                                                                                                       ttGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATAAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAAAGCCAAGATCTTTTTCCAGATGt                          >  2:24308/1‑149 (MQ=255)
                                                                                                                         gagaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTg                        >  1:1806353/1‑149 (MQ=255)
                                                                                                                          agaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTgg                       >  1:1306439/1‑149 (MQ=255)
                                                                                                                          agaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTgg                       <  1:93683/149‑1 (MQ=255)
                                                                                                                             aaTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCa                    <  2:3123925/149‑1 (MQ=255)
                                                                                                                             aaTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCa                    <  2:2898105/149‑1 (MQ=255)
                                                                                                                               tCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaaa                  >  1:300582/1‑149 (MQ=255)
                                                                                                                               tCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaaa                  >  2:3604720/1‑149 (MQ=255)
                                                                                                                               tCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaaa                  >  1:3810460/1‑149 (MQ=255)
                                                                                                                                  tATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTc               >  1:3538202/1‑149 (MQ=255)
                                                                                                                                       tttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACtt          <  1:2513146/149‑1 (MQ=255)
                                                                                                                                        ttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTc         <  2:206435/149‑1 (MQ=255)
                                                                                                                                          cTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaa       >  2:1176848/1‑149 (MQ=255)
                                                                                                                                          cTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaa       >  2:1177131/1‑149 (MQ=255)
                                                                                                                                           ttGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaa      >  1:3124543/1‑149 (MQ=255)
                                                                                                                                            tGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaaa     <  1:682327/149‑1 (MQ=255)
                                                                                                                                             gcgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAc    <  1:1462016/149‑1 (MQ=255)
                                                                                                                                              cgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAcc   >  1:3959952/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCG  >  CP009273/1993384‑1993675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑