BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,040,800	C→A	7.4%	T794T (ACC→ACA)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,040,800	0	C	A	7.4%	830.5 / 27.1	228	T794T (ACC→ACA)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base C (106/105); new base A (6/11); total (112/116)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 229 total aligned reads displayed.

AGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATC  >  CP009273/2040654‑2040948

aGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGc                                                                                                                                                     <  2:4107345/149‑1 (MQ=255)

 gCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCTACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑a                                                                                                                                                   >  2:3033972/1‑149 (MQ=255)

  cAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAAGACCGC‑aa                                                                                                                                                  <  1:185076/149‑1 (MQ=255)

    ataatGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑aaaa                                                                                                                                                >  1:2313318/1‑149 (MQ=255)

     taatGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑aaaaa                                                                                                                                               >  1:2744578/1‑149 (MQ=255)

           tgtgCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATg                                                                                                                                         >  1:2430834/1‑149 (MQ=255)

           tgtgCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATg                                                                                                                                         >  1:742257/1‑149 (MQ=255)

              gCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTa                                                                                                                                      >  2:2478659/1‑149 (MQ=255)

              gCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACAGC‑AAAAACGGATGTTa                                                                                                                                      >  1:791705/1‑149 (MQ=255)

                tCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAAt                                                                                                                                    <  1:3998579/149‑1 (MQ=255)

                  gCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATgg                                                                                                                                  >  1:2736187/1‑149 (MQ=255)

                  gCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATgg                                                                                                                                  >  1:2034421/1‑149 (MQ=255)

                     aaTGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCt                                                                                                                               <  1:3381779/149‑1 (MQ=255)

                      aTGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTg                                                                                                                              >  1:3675233/1‑149 (MQ=255)

                      aTGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTg                                                                                                                              >  1:80789/1‑149 (MQ=255)

                       tGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGACAACTTCCTTCAACAATCAAAACACAGC‑AAAAACGGATGTTAATGGTCTgg                                                                                                                             >  1:336489/1‑149 (MQ=255)

                            aTGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTGTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACt                                                                                                                        <  2:2606096/149‑1 (MQ=255)

                                 gcaAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAAACGGATGTTAATGGTCTGGCGACTTTTg                                                                                                                    >  2:3229360/1‑149 (MQ=255)

                                 gcaAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGa                                                                                                                   >  2:3230128/1‑149 (MQ=255)

                                 gcaAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGa                                                                                                                   >  2:2202877/1‑149 (MQ=255)

                                  caAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGAt                                                                                                                  <  1:3752998/149‑1 (MQ=255)

                                     acacCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTg                                                                                                               >  1:3876972/1‑149 (MQ=255)

                                       acCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGaa                                                                                                             >  2:1309652/1‑149 (MQ=255)

                                       acCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGaa                                                                                                             >  1:1673304/1‑149 (MQ=255)

                                         cGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGCTGTTAATGGTCTGGCGACTTTTGATCTGaaaa                                                                                                           >  2:3975960/1‑149 (MQ=255)

                                         cGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGaaaa                                                                                                           >  2:1710766/1‑149 (MQ=255)

                                               ggTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAgtagta                                                                                                     <  2:622386/149‑1 (MQ=255)

                                                gTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGAGACTTTTGATCTGATAAGTAGTaa                                                                                                    <  1:930558/149‑1 (MQ=255)

                                                 tCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAg                                                                                                   >  1:2759978/1‑149 (MQ=255)

                                                 tCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAg                                                                                                   >  1:2761288/1‑149 (MQ=255)

                                                 tCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAg                                                                                                   <  2:3759591/149‑1 (MQ=255)

                                                    aTGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAg                                                                                                >  1:1076772/1‑149 (MQ=255)

                                                     tGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAgg                                                                                               >  1:1196471/1‑149 (MQ=255)

                                                     tGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACAGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAgg                                                                                               <  1:3052910/149‑1 (MQ=255)

                                                      gTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGa                                                                                              <  1:1279404/149‑1 (MQ=255)

                                                       tCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGTGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGaa                                                                                             >  2:3827106/1‑149 (MQ=255)

                                                        cGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAg                                                                                            <  1:1396946/149‑1 (MQ=255)

                                                         gCTGATGAAGGAAGCAACCCAATCACTGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGa                                                                                           <  2:785321/149‑1 (MQ=255)

                                                         gCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGa                                                                                           >  2:881573/1‑149 (MQ=255)

                                                            gatgaAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGacaa                                                                                        <  2:2734137/149‑1 (MQ=255)

                                                             atgaAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGacaac                                                                                       <  1:270594/149‑1 (MQ=255)

                                                              tgaAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGacaaca                                                                                      <  2:3650992/149‑1 (MQ=255)

                                                               gaaggaagCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAacac                                                                                     >  1:2948504/1‑149 (MQ=255)

                                                                    aagCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTg                                                                                <  2:3285466/149‑1 (MQ=255)

                                                                        aaCCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACAGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGt                                                                            <  2:1494886/149‑1 (MQ=255)

                                                                        aaCCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACAGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGt                                                                            <  2:1494764/149‑1 (MQ=255)

                                                                            caatcaatGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCAcc                                                                        <  1:1497708/149‑1 (MQ=255)

                                                                            caatcaatGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCAcc                                                                        >  2:3107474/1‑149 (MQ=255)

                                                                             aatcaatGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCAccc                                                                       <  2:2365597/149‑1 (MQ=255)

                                                                               tcaatGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCtt                                                                     <  1:3931010/149‑1 (MQ=255)

                                                                               tcaatGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCtt                                                                     <  2:3169398/149‑1 (MQ=255)

                                                                                caatGATCAGACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACAGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTg                                                                    <  1:2331472/149‑1 (MQ=255)

                                                                                  atGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGaa                                                                  >  1:563519/1‑149 (MQ=255)

                                                                                      tCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATg                                                              <  1:1320072/149‑1 (MQ=255)

                                                                                        aTACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGc                                                            <  2:4012629/149‑1 (MQ=255)

                                                                                        aTACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGc                                                            <  1:2102030/149‑1 (MQ=255)

                                                                                        aTACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGc                                                            >  2:115071/1‑149 (MQ=255)

                                                                                          aCCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGt                                                          <  1:809299/149‑1 (MQ=255)

                                                                                             gTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGaa                                                       >  2:1057012/1‑149 (MQ=255)

                                                                                             gTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGaa                                                       >  1:987235/1‑149 (MQ=255)

                                                                                               cACGTTTGCGTTATTATGCGGATCGGCAACGTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAATATGGCGTGaaac                                                     <  2:158164/149‑1 (MQ=255)

                                                                                                aCGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGaaaca                                                    <  2:2567733/149‑1 (MQ=255)

                                                                                                   tttGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGaaacaaac                                                 >  1:79546/1‑149 (MQ=255)

                                                                                                    ttGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACg                                                <  2:4082424/149‑1 (MQ=255)

                                                                                                     tGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGt                                               <  1:1190086/149‑1 (MQ=255)

                                                                                                        ggTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTaa                                            >  1:1912523/1‑149 (MQ=255)

                                                                                                            ttcaGCGGATCGGCAACGTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGt                                        <  2:2112925/146‑1 (MQ=255)

                                                                                                            ttAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGt                                        >  1:2253677/1‑149 (MQ=255)

                                                                                                              aaGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCa                                      <  2:1196288/149‑1 (MQ=255)

                                                                                                                  ggATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGttt                                  >  1:654844/1‑149 (MQ=255)

                                                                                                                   gATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGtttt                                 >  1:1149854/1‑149 (MQ=255)

                                                                                                                    aTCGTCAACTTCCTTCAACAATCAAAACACCCC‑AAAACCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTg                                <  1:4058678/149‑1 (MQ=255)

                                                                                                                     tCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGt                               <  2:2337205/149‑1 (MQ=255)

                                                                                                                     tCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGt                               <  2:2337106/149‑1 (MQ=255)

                                                                                                                     tCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGt                               <  2:2868242/149‑1 (MQ=255)

                                                                                                                      cGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTc                              <  2:1214841/149‑1 (MQ=255)

                                                                                                                      cGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTc                              <  2:3989085/149‑1 (MQ=255)

                                                                                                                       ggCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCg                             >  2:1928955/1‑149 (MQ=255)

                                                                                                                       ggCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTGTTGATCTGACAAGTAGAAAGAAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTGTTGTCg                             >  2:1929063/1‑149 (MQ=255)

                                                                                                                        gCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGACAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCgg                            >  1:3920428/1‑149 (MQ=255)

                                                                                                                        gCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCgg                            >  1:3455109/1‑149 (MQ=255)

                                                                                                                           aCTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGa                         <  1:3022789/149‑1 (MQ=255)

                                                                                                                           aCTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGa                         >  1:1578208/1‑149 (MQ=255)

                                                                                                                           aCTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGa                         >  1:1979319/1‑149 (MQ=255)

                                                                                                                              tccttcAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTc                      <  2:2736274/149‑1 (MQ=255)

                                                                                                                               ccttcAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCg                     >  1:3035883/1‑149 (MQ=255)

                                                                                                                                cttcAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGa                    <  1:3751943/149‑1 (MQ=255)

                                                                                                                                cttcAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGa                    <  1:3751743/149‑1 (MQ=255)

                                                                                                                                cttcAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGa                    >  1:959431/1‑149 (MQ=255)

                                                                                                                                   caacaaTCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTa                 <  1:2215172/149‑1 (MQ=255)

                                                                                                                                    aacaaTCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTAc                <  1:317700/149‑1 (MQ=255)

                                                                                                                                    aacaaTCAAAACACAGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTAc                >  2:30830/1‑149 (MQ=255)

                                                                                                                                         tCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgcgc           <  2:605679/149‑1 (MQ=255)

                                                                                                                                         tCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgcgc           <  2:605723/149‑1 (MQ=255)

                                                                                                                                          cAAAACACAGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCa          <  2:791705/149‑1 (MQ=255)

                                                                                                                                            aaaCACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAgg        >  1:1705238/1‑149 (MQ=255)

                                                                                                                                            aaaCACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAgg        >  2:445041/1‑149 (MQ=255)

                                                                                                                                             aaCACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGt       >  1:2370526/1‑149 (MQ=255)

                                                                                                                                             aaCACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGt       >  2:244119/1‑149 (MQ=255)

                                                                                                                                             aaCACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGGTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGt       >  2:3534953/1‑149 (MQ=255)

AGCAATAATGGTGTGCTCGCCAATGAGAATGCAGCAAACACCGTCTCGGTCAATGTCGCTGATGAAGGAAGCAACCCAATCAATGATCATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGC‑AAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATC  >  CP009273/2040654‑2040948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`