Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,041,346 C→A 100% I976I (ATC→ATA)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,041,3460CA95.3% 742.6 / 26.1 254I976I (ATC→ATA) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (6/6);  new base A (143/99);  total (149/105)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 254 total aligned reads displayed.

CAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAACA  >  CP009273/2041200‑2041491
                                                                                                                                                  |                                                                                                                                                 
cAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAc                                                                                                                                                 <  1:706799/149‑1 (MQ=255)
cAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAc                                                                                                                                                 <  1:3091731/149‑1 (MQ=255)
 aGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAcc                                                                                                                                                >  2:419501/1‑149 (MQ=255)
 aGGTGAATTTGATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAAcc                                                                                                                                                <  2:2220379/149‑1 (MQ=255)
  ggTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCt                                                                                                                                               >  2:3497936/1‑149 (MQ=255)
  ggTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCt                                                                                                                                               <  2:2871931/149‑1 (MQ=255)
    tGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtc                                                                                                                                             <  2:1057808/149‑1 (MQ=255)
     gAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtct                                                                                                                                            >  1:3166458/1‑149 (MQ=255)
      aaTTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCCGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtctc                                                                                                                                           <  2:238615/149‑1 (MQ=255)
      aaTTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtctc                                                                                                                                           <  2:3732885/149‑1 (MQ=255)
       aTTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATTACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATACCCTTctct                                                                                                                                          <  1:1966902/149‑1 (MQ=255)
        aattAACGGTGATCAAAGTACAGCTGCACAGACACTCAGTGTGCCTACACGTCAAATCAACGACACCAACACAGCTCCGCAATATATCACACAAACCTTGCAGTAAAAAAAAGTCAACCCACAAAAAGATAAACAAATAACCTTCACtg                                                                                                                                         >  2:570701/3‑149 (MQ=255)
         tttATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCtgt                                                                                                                                        >  1:1234635/1‑149 (MQ=255)
         tttATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCtgt                                                                                                                                        >  1:1323344/1‑149 (MQ=255)
             tCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCa                                                                                                                                    >  2:919783/1‑149 (MQ=255)
                gTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAac                                                                                                                                 >  1:1052439/1‑149 (MQ=255)
                  gATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacga                                                                                                                               <  1:207411/149‑1 (MQ=255)
                    tCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacgacg                                                                                                                             >  1:778145/1‑149 (MQ=255)
                    tCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacgacg                                                                                                                             <  1:3041773/149‑1 (MQ=255)
                       aaGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAACAGATACAGAAATAACCTTCTCTGTGCCAAACGACGTCg                                                                                                                          >  2:3573551/1‑149 (MQ=255)
                                tgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagtaagt                                                                                                                 <  2:1734128/149‑1 (MQ=255)
                                 gcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGtt                                                                                                                >  2:1879305/1‑149 (MQ=255)
                                  cccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtc                                                                                                               <  1:2705645/149‑1 (MQ=255)
                                   ccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTtct                                                                                                              <  1:2838578/149‑1 (MQ=255)
                                      gACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGa                                                                                                           <  2:3366678/149‑1 (MQ=255)
                                      gACCCTCAGTGTGCATTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGTATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGa                                                                                                           <  1:387161/149‑1 (MQ=255)
                                        cccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAtt                                                                                                         <  2:2628302/149‑1 (MQ=255)
                                        cccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATAGGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAtt                                                                                                         <  2:2672168/149‑1 (MQ=255)
                                         ccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTa                                                                                                        >  2:1029120/1‑149 (MQ=255)
                                         ccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTa                                                                                                        >  2:605193/1‑149 (MQ=255)
                                           tCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGc                                                                                                      >  1:968064/1‑149 (MQ=255)
                                           tCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGc                                                                                                      >  2:3247965/1‑149 (MQ=255)
                                           tCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGc                                                                                                      >  2:4059531/1‑149 (MQ=255)
                                             aGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCaa                                                                                                    <  1:179636/149‑1 (MQ=255)
                                             aGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCaa                                                                                                    <  1:3874136/149‑1 (MQ=255)
                                                gtgCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgg                                                                                                 >  1:3200295/1‑149 (MQ=255)
                                                gtgCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgg                                                                                                 >  1:3204425/1‑149 (MQ=255)
                                                 tgCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACgga                                                                                                >  2:2933835/1‑149 (MQ=255)
                                                  gCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACggag                                                                                               <  2:660228/149‑1 (MQ=255)
                                                   ccTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACggagg                                                                                              <  1:681353/149‑1 (MQ=255)
                                                        aGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAg                                                                                         >  1:1306405/1‑149 (MQ=255)
                                                        aGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAg                                                                                         >  1:2438141/1‑149 (MQ=255)
                                                        aGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAg                                                                                         >  1:2218585/1‑149 (MQ=255)
                                                          gTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGc                                                                                       >  1:335112/1‑149 (MQ=255)
                                                               atCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGAc                                                                                  <  2:2329710/149‑1 (MQ=255)
                                                                  aCCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGa                                                                               >  1:529170/1‑149 (MQ=255)
                                                                   ccGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGAt                                                                              <  1:271941/149‑1 (MQ=255)
                                                                   ccGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGAt                                                                              <  2:729668/149‑1 (MQ=255)
                                                                      tCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGt                                                                           >  1:3479493/1‑149 (MQ=255)
                                                                       cACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                          <  2:2480380/149‑1 (MQ=255)
                                                                        aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTaa                                                                         >  2:4083912/1‑149 (MQ=255)
                                                                        aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTaa                                                                         >  1:1018396/1‑149 (MQ=255)
                                                                         ccAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAAc                                                                        <  2:4041154/149‑1 (MQ=255)
                                                                            ncacaGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACggg                                                                     >  1:3523754/2‑149 (MQ=255)
                                                                            acacAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACggg                                                                     >  1:1477288/1‑149 (MQ=255)
                                                                               caGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGtt                                                                  >  2:3763133/1‑149 (MQ=255)
                                                                                aGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTg                                                                 >  2:2050306/1‑149 (MQ=255)
                                                                                   tCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCaa                                                              >  2:3450466/1‑149 (MQ=255)
                                                                                        aaTATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGcc                                                         >  1:2920583/1‑149 (MQ=255)
                                                                                        aaTATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGcc                                                         >  2:1763377/1‑149 (MQ=255)
                                                                                            tatGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTccc                                                     <  2:3035081/149‑1 (MQ=255)
                                                                                                aCTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGAc                                                 >  2:3449007/1‑149 (MQ=255)
                                                                                                    cAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGc                                             >  1:1022904/1‑149 (MQ=255)
                                                                                                      aCCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCAc                                           >  2:3918578/1‑149 (MQ=255)
                                                                                                      aCCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCAc                                           <  1:39496/149‑1 (MQ=255)
                                                                                                       ccTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACg                                          >  1:1206914/1‑149 (MQ=255)
                                                                                                        cTTGCAGGATAAAAATGGCAACCCACTAAACGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGt                                         <  1:2399470/149‑1 (MQ=255)
                                                                                                           gCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAACTAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAg                                      <  1:3913482/149‑1 (MQ=255)
                                                                                                            cAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGc                                     <  2:3200295/149‑1 (MQ=255)
                                                                                                            cAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGc                                     >  1:138166/1‑149 (MQ=255)
                                                                                                             aGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATACCCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCg                                    <  1:1311622/149‑1 (MQ=255)
                                                                                                              gtaTAAAAATGGAAACCCACTAAAAGATAAAGAAATAACCTACTCTGTGACAAAAGACGTCGCAAGTAAGTTCTCGAGTAGCAACGGAGGAAAAGGCATGACTGATATTAACGCGTTTGCAATCGCCTCCCTGACCGTCACGTTAGAgg                                   >  1:1641301/3‑149 (MQ=255)
                                                                                                              ggATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCgg                                   >  1:3685637/1‑149 (MQ=255)
                                                                                                                aTAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGc                                 >  2:2866181/1‑149 (MQ=255)
                                                                                                                aTAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGc                                 <  2:1750935/149‑1 (MQ=255)
                                                                                                                 tAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCa                                >  2:1081393/1‑149 (MQ=255)
                                                                                                                 tAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCa                                <  1:2275907/149‑1 (MQ=255)
                                                                                                                 tAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCa                                <  1:3743500/149‑1 (MQ=255)
                                                                                                                   aaaaTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACg                              >  2:3200988/1‑149 (MQ=255)
                                                                                                                        ggCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCatat                         <  2:1196581/149‑1 (MQ=255)
                                                                                                                           aaCCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGAt                      >  1:1875582/1‑149 (MQ=255)
                                                                                                                           aaCCCACTAAAAGATAAAGAAATAACCTTCTCTGTACCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGAt                      >  2:69069/1‑149 (MQ=255)
                                                                                                                            aCCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATc                     <  2:136540/149‑1 (MQ=255)
                                                                                                                            aCCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATc                     >  1:299452/1‑149 (MQ=255)
                                                                                                                                aCTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATgg                 <  2:3880024/149‑1 (MQ=255)
                                                                                                                                 cTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGc                >  2:1582798/1‑149 (MQ=255)
                                                                                                                                 cTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGc                >  1:2363192/1‑149 (MQ=255)
                                                                                                                                   aaaaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTc              >  2:1502044/1‑149 (MQ=255)
                                                                                                                                   aaaaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTc              <  1:620063/149‑1 (MQ=255)
                                                                                                                                     aaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt            >  1:1766816/1‑149 (MQ=255)
                                                                                                                                      aGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCATCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTc           >  1:1898134/1‑149 (MQ=255)
                                                                                                                                       gATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCt          >  1:2633924/1‑149 (MQ=255)
                                                                                                                                         tAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg        >  2:3126601/1‑149 (MQ=255)
                                                                                                                                         tAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg        <  2:13202/149‑1 (MQ=255)
                                                                                                                                         tAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg        >  2:1212020/1‑149 (MQ=255)
                                                                                                                                         tAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg        >  1:2288104/1‑149 (MQ=255)
                                                                                                                                         tAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg        >  1:215033/1‑149 (MQ=255)
                                                                                                                                          aaaGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGc       >  1:3153680/1‑149 (MQ=255)
                                                                                                                                           aaGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCt      >  1:1037721/1‑149 (MQ=255)
                                                                                                                                             gAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTaa    >  1:1363961/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAACA  >  CP009273/2041200‑2041491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑