Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,049,111 C→A 100% T190T (ACC→ACA)  amn → AMP nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,049,1110CA99.6% 845.8 / ‑4.2 260T190T (ACC→ACA) amnAMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (118/141);  total (119/141)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 260 total aligned reads displayed.

TAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGG  >  CP009273/2048966‑2049255
                                                                                                                                                 |                                                                                                                                                
tAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGt                                                                                                                                               <  1:796662/149‑1 (MQ=255)
 aaCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                              <  2:414958/149‑1 (MQ=255)
 aaCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                              <  1:1435263/149‑1 (MQ=255)
 aaCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                              <  2:2774052/149‑1 (MQ=255)
 aaCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGCTTGCGCCATTATACAGGTa                                                                                                                                              <  1:2573722/149‑1 (MQ=255)
  aCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTAc                                                                                                                                             >  1:513587/1‑149 (MQ=255)
        tACGTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGt                                                                                                                                       <  1:1469202/149‑1 (MQ=255)
          cTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTg                                                                                                                                     >  1:1810247/1‑149 (MQ=255)
           ttCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGa                                                                                                                                    <  2:3727396/149‑1 (MQ=255)
            tCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGaa                                                                                                                                   <  1:1945335/149‑1 (MQ=255)
             cccGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAAc                                                                                                                                  <  2:2646528/149‑1 (MQ=255)
              ccGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACa                                                                                                                                 <  1:3489338/149‑1 (MQ=255)
               cGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAt                                                                                                                                <  1:741205/149‑1 (MQ=255)
                gACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtt                                                                                                                               <  2:1865064/149‑1 (MQ=255)
                  caacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtttt                                                                                                                             <  1:1248969/149‑1 (MQ=255)
                    acaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCa                                                                                                                           >  2:1493613/1‑149 (MQ=255)
                      aGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGc                                                                                                                         <  2:3553751/149‑1 (MQ=255)
                      aGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGc                                                                                                                         >  1:3397212/1‑149 (MQ=255)
                      aGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTATCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGc                                                                                                                         >  1:3714997/1‑149 (MQ=255)
                           tGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTc                                                                                                                    >  1:1720678/1‑149 (MQ=255)
                               gcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCt                                                                                                                >  2:319121/1‑149 (MQ=255)
                               gcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCt                                                                                                                <  1:3620251/149‑1 (MQ=255)
                               gcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGAGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCt                                                                                                                >  2:1815002/1‑149 (MQ=255)
                                 aaaTTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTg                                                                                                              <  1:1160606/149‑1 (MQ=255)
                                    ttGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGttt                                                                                                           <  2:2093668/149‑1 (MQ=255)
                                    ttGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGttt                                                                                                           <  1:132672/149‑1 (MQ=255)
                                     tGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTa                                                                                                          >  2:1988799/1‑149 (MQ=255)
                                      ggCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTAc                                                                                                         <  2:2192942/149‑1 (MQ=255)
                                         gATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCaa                                                                                                      >  2:3819075/1‑149 (MQ=255)
                                           tGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACt                                                                                                    >  1:3747838/1‑149 (MQ=255)
                                            gAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTa                                                                                                   >  1:2701229/1‑149 (MQ=255)
                                               aCTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTGCGTCTTGTTTACCAACTacac                                                                                                >  2:2673187/1‑149 (MQ=255)
                                                cTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacaca                                                                                               <  2:3014452/149‑1 (MQ=255)
                                                     gACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          <  1:3031321/149‑1 (MQ=255)
                                                         gCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtgtg                                                                                      <  2:1560928/149‑1 (MQ=255)
                                                         gCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtgtg                                                                                      >  2:1491030/1‑149 (MQ=255)
                                                          cATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTgg                                                                                     <  1:4036765/149‑1 (MQ=255)
                                                           aTTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGa                                                                                    <  2:3789177/149‑1 (MQ=255)
                                                            tttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  1:3258212/1‑149 (MQ=255)
                                                            tttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  2:1238682/1‑149 (MQ=255)
                                                             ttATCATCCTACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATg                                                                                  <  2:2468377/149‑1 (MQ=255)
                                                             ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATg                                                                                  <  2:2474906/149‑1 (MQ=255)
                                                              tATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGa                                                                                 >  1:1561378/1‑149 (MQ=255)
                                                               atcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATgta                                                                                >  2:2368045/1‑147 (MQ=255)
                                                               atcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGaa                                                                                >  2:2366972/1‑149 (MQ=255)
                                                                tcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAt                                                                               <  1:478514/149‑1 (MQ=255)
                                                                 catcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAtt                                                                              <  2:207731/149‑1 (MQ=255)
                                                                 catcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAtt                                                                              >  2:3939899/1‑149 (MQ=255)
                                                                  atcCAACTGAAGTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttc                                                                             <  2:4075421/149‑1 (MQ=255)
                                                                   tcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTAACAACTACACACGTTATGTGGATGAAttcg                                                                            >  1:982793/1‑149 (MQ=255)
                                                                     cAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgtt                                                                          <  1:3545246/149‑1 (MQ=255)
                                                                     cAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgtt                                                                          <  1:2851784/149‑1 (MQ=255)
                                                                     cAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgtt                                                                          <  2:1565807/149‑1 (MQ=255)
                                                                      aaCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgttc                                                                         <  2:3779884/149‑1 (MQ=255)
                                                                       aCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgttcg                                                                        >  2:2500185/1‑149 (MQ=255)
                                                                           aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgg                                                                    <  1:1536953/149‑1 (MQ=255)
                                                                            aTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTggg                                                                   >  1:4061657/1‑149 (MQ=255)
                                                                               ctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGAt                                                                >  2:2956801/1‑149 (MQ=255)
                                                                               ctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGAt                                                                <  1:3582579/149‑1 (MQ=255)
                                                                                 ccccGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGc                                                              <  1:3518128/149‑1 (MQ=255)
                                                                                 ccccGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTAGTTGGGGATGc                                                              >  1:2770278/1‑149 (MQ=255)
                                                                                   ccGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAg                                                            >  2:3753934/1‑149 (MQ=255)
                                                                                   ccGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAg                                                            >  2:3413078/1‑149 (MQ=255)
                                                                                     gCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGcc                                                          >  1:1873118/1‑149 (MQ=255)
                                                                                     gCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGcc                                                          <  2:814842/149‑1 (MQ=255)
                                                                                        aTCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAGGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGa                                                       >  2:3662280/1‑149 (MQ=255)
                                                                                         tCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGAt                                                      <  2:1288831/149‑1 (MQ=255)
                                                                                         tCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGAt                                                      <  1:893578/149‑1 (MQ=255)
                                                                                         tCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGAt                                                      <  2:86451/149‑1 (MQ=255)
                                                                                         tCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGGTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGAt                                                      >  1:1288212/1‑149 (MQ=255)
                                                                                          cGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATc                                                     <  1:2170799/149‑1 (MQ=255)
                                                                                           gCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATcc                                                    <  1:1043246/149‑1 (MQ=255)
                                                                                            cATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCt                                                   <  2:731624/149‑1 (MQ=255)
                                                                                             aTTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTc                                                  >  2:2234110/1‑149 (MQ=255)
                                                                                             aTTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGGTGGGGATGCAGCCAGATCCTc                                                  >  2:3269718/1‑149 (MQ=255)
                                                                                                 tGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATc                                              <  2:2902641/149‑1 (MQ=255)
                                                                                                     gcgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGa                                          <  1:2435129/149‑1 (MQ=255)
                                                                                                     gcgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGa                                          <  2:3537370/149‑1 (MQ=255)
                                                                                                     gcgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGa                                          <  1:1621991/149‑1 (MQ=255)
                                                                                                      cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACCCGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGAt                                         <  2:1124389/149‑1 (MQ=255)
                                                                                                       gcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATa                                        >  1:2269250/1‑149 (MQ=255)
                                                                                                         gccgcGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGt                                      <  2:1873118/149‑1 (MQ=255)
                                                                                                               tCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTAc                                >  2:1613433/1‑149 (MQ=255)
                                                                                                                 gATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACAt                              <  2:4014331/149‑1 (MQ=255)
                                                                                                                  aTTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACAtt                             >  2:350190/1‑149 (MQ=255)
                                                                                                                  aTTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACAtt                             <  2:3933157/149‑1 (MQ=255)
                                                                                                                   tttttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTg                            >  2:827750/1‑149 (MQ=255)
                                                                                                                     tttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGcc                          >  2:3618615/1‑149 (MQ=255)
                                                                                                                       tCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGTACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTAGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCt                        <  1:410178/149‑1 (MQ=255)
                                                                                                                        cccTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCtt                       >  2:2282436/1‑149 (MQ=255)
                                                                                                                        cccTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCtt                       >  2:62526/1‑149 (MQ=255)
                                                                                                                          cTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTc                     >  1:2104662/1‑149 (MQ=255)
                                                                                                                             gCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtg                  <  2:1461868/149‑1 (MQ=255)
                                                                                                                             gCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtg                  >  1:3344069/1‑149 (MQ=255)
                                                                                                                               aCGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGGTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtgtg                >  2:2834184/1‑149 (MQ=255)
                                                                                                                                       gcCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCggg        <  1:2193495/149‑1 (MQ=255)
                                                                                                                                         cATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGaa      >  2:1957565/1‑149 (MQ=255)
                                                                                                                                          aTTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAAc     >  1:2766058/1‑149 (MQ=255)
                                                                                                                                           ttATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACt    >  1:2589943/1‑149 (MQ=255)
                                                                                                                                            tataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTg   >  1:17734/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGG  >  CP009273/2048966‑2049255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑