Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 208,013 G→T 100% M616I (ATG→ATT)  ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273208,0130GT97.8% 732.0 / 5.9 229M616I (ATG→ATT) ldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (115/109);  total (116/113)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

AATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATG  >  CP009273/207867‑208157
                                                                                                                                                  |                                                                                                                                                
    tGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATg                                                                                                                                            >  1:3645555/1‑149 (MQ=255)
      cTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGAc                                                                                                                                          >  1:2452413/1‑149 (MQ=255)
      cTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGAc                                                                                                                                          <  1:578968/149‑1 (MQ=255)
       tACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACg                                                                                                                                         >  2:3196769/1‑149 (MQ=255)
        aCCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGc                                                                                                                                        <  2:883824/149‑1 (MQ=255)
        aCCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGc                                                                                                                                        <  2:848952/149‑1 (MQ=255)
          ccGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCca                                                                                                                                      >  1:1936577/1‑149 (MQ=255)
           cGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCcac                                                                                                                                     >  2:2474611/1‑149 (MQ=255)
              tgtctATGCAGAAGATCCCGATTTGTTGCGCAATATGCGTATTCAGGATGTGGCACATGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGTTACTTTTCCGGAGATTATCATGACGCCACATc                                                                                                                                  <  1:1312865/147‑1 (MQ=255)
              tctcTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATc                                                                                                                                  >  1:1064621/1‑149 (MQ=255)
              tctcTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATc                                                                                                                                  >  2:1020857/1‑149 (MQ=255)
                tctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACTCCACATCAg                                                                                                                                <  2:3567008/149‑1 (MQ=255)
                 ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAgg                                                                                                                               >  1:2771011/1‑149 (MQ=255)
                  tATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGc                                                                                                                              <  2:4040937/149‑1 (MQ=255)
                   aTGCAGAAGATCCCGATTTCTACCGCACTATGAGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCa                                                                                                                             <  1:2580032/149‑1 (MQ=255)
                       agaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGc                                                                                                                         >  2:2564657/1‑149 (MQ=255)
                       agaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGc                                                                                                                         <  2:2941424/149‑1 (MQ=255)
                        gaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCa                                                                                                                        >  2:3872817/1‑149 (MQ=255)
                        gaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCa                                                                                                                        >  2:1399620/1‑149 (MQ=255)
                        gaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCa                                                                                                                        >  2:3872866/1‑149 (MQ=255)
                         aagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCaa                                                                                                                       >  1:718438/1‑149 (MQ=255)
                         aagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCaa                                                                                                                       <  1:1794875/149‑1 (MQ=255)
                          agaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAAc                                                                                                                      <  2:2726184/149‑1 (MQ=255)
                            aTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGa                                                                                                                    <  2:876248/149‑1 (MQ=255)
                            aTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGa                                                                                                                    >  1:1457309/1‑149 (MQ=255)
                               ccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACaa                                                                                                                 <  1:2137167/149‑1 (MQ=255)
                                cGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACaaa                                                                                                                <  2:1665167/149‑1 (MQ=255)
                                 gATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAAt                                                                                                               >  1:1592437/1‑149 (MQ=255)
                                  aTTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAAtt                                                                                                              >  2:819286/1‑149 (MQ=255)
                                     tCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTaaa                                                                                                           <  1:268984/149‑1 (MQ=255)
                                      cTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAg                                                                                                          <  1:1431515/149‑1 (MQ=255)
                                       tACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAgg                                                                                                         >  2:719640/1‑149 (MQ=255)
                                        aCCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGc                                                                                                        >  1:3868108/1‑149 (MQ=255)
                                         ccGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCg                                                                                                       >  1:1495471/1‑149 (MQ=255)
                                          cGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGa                                                                                                      <  1:2600987/149‑1 (MQ=255)
                                          cGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGa                                                                                                      >  2:1574550/1‑149 (MQ=255)
                                           gCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGaa                                                                                                     <  1:3196769/149‑1 (MQ=255)
                                            cAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAg                                                                                                    <  2:2089415/149‑1 (MQ=255)
                                             aaTATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGt                                                                                                   >  1:520673/1‑149 (MQ=255)
                                              atatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTa                                                                                                  >  2:299867/1‑149 (MQ=255)
                                                 tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGaa                                                                                               >  2:1771547/1‑149 (MQ=255)
                                                   cGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAAc                                                                                             <  1:3795181/149‑1 (MQ=255)
                                                    gTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAAcc                                                                                            >  1:2831440/1‑149 (MQ=255)
                                                    gTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAAcc                                                                                            >  1:1892973/1‑149 (MQ=255)
                                                      aTTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAt                                                                                          <  2:3572129/149‑1 (MQ=255)
                                                      aTTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAt                                                                                          >  1:3169566/1‑149 (MQ=255)
                                                           ggATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTgcgc                                                                                     >  2:2277212/1‑149 (MQ=255)
                                                           ggATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTgcgc                                                                                     >  2:185941/1‑149 (MQ=255)
                                                            gATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAGTGCGCt                                                                                    <  2:1676418/149‑1 (MQ=255)
                                                                    acaAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGaacaac                                                                            <  1:1163515/149‑1 (MQ=255)
                                                                     caAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACt                                                                           <  2:1865501/149‑1 (MQ=255)
                                                                       aGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTgg                                                                         <  2:3222387/149‑1 (MQ=255)
                                                                        gggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGt                                                                        >  2:3165538/1‑149 (MQ=255)
                                                                        gggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGt                                                                        >  1:2262245/1‑149 (MQ=255)
                                                                         ggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTc                                                                       <  1:1703139/149‑1 (MQ=255)
                                                                            tCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGt                                                                    <  2:1385006/149‑1 (MQ=255)
                                                                                tAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTagag                                                                <  1:2648749/149‑1 (MQ=255)
                                                                                 aaGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGt                                                               >  1:440099/1‑149 (MQ=255)
                                                                                  aGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTa                                                              <  2:906899/149‑1 (MQ=255)
                                                                                   gCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTAt                                                             >  2:1629764/1‑149 (MQ=255)
                                                                                   gCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTAt                                                             <  2:440099/149‑1 (MQ=255)
                                                                                    cTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATc                                                            >  2:2338233/1‑149 (MQ=255)
                                                                                      gATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCgg                                                          >  1:853186/1‑149 (MQ=255)
                                                                                      gATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCgg                                                          >  2:2287858/1‑149 (MQ=255)
                                                                                        ttCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCa                                                        >  2:2007779/1‑149 (MQ=255)
                                                                                           gTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAat                                                     <  2:1226955/149‑1 (MQ=255)
                                                                                            tAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAata                                                    >  1:2052287/1‑149 (MQ=255)
                                                                                            tAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACTCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAata                                                    <  1:2991556/149‑1 (MQ=255)
                                                                                              aaCACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATg                                                  >  1:156652/1‑149 (MQ=255)
                                                                                                cacGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGAt                                                <  2:1069027/149‑1 (MQ=255)
                                                                                                 acGATCTTCCCGTTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATc                                               <  1:2801419/149‑1 (MQ=255)
                                                                                                 acGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATc                                               <  2:2707910/149‑1 (MQ=255)
                                                                                                    aTCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTg                                            >  1:3330697/1‑149 (MQ=255)
                                                                                                    aTCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTg                                            >  1:1280937/1‑149 (MQ=255)
                                                                                                    aTCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTg                                            >  1:194995/1‑149 (MQ=255)
                                                                                                     tCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGc                                           <  2:142718/149‑1 (MQ=255)
                                                                                                       ttCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCt                                         <  2:1143777/149‑1 (MQ=255)
                                                                                                           cGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATc                                     <  1:3375719/149‑1 (MQ=255)
                                                                                                             gTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCa                                   >  2:94202/1‑149 (MQ=255)
                                                                                                              tttGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCAc                                  >  1:3272999/1‑149 (MQ=255)
                                                                                                                 gATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCgg                               >  1:3972662/1‑149 (MQ=255)
                                                                                                                   tGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGc                             <  1:1884983/149‑1 (MQ=255)
                                                                                                                      tGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTa                          >  2:2866009/1‑149 (MQ=255)
                                                                                                                       gCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTAc                         <  1:174865/149‑1 (MQ=255)
                                                                                                                        cGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTAcc                        <  2:3645555/149‑1 (MQ=255)
                                                                                                                          ggCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGc                      <  1:2564657/149‑1 (MQ=255)
                                                                                                                          ggCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGc                      <  2:3303049/149‑1 (MQ=255)
                                                                                                                          ggCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGc                      >  2:2832891/1‑149 (MQ=255)
                                                                                                                           gCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCt                     >  2:1728351/1‑149 (MQ=255)
                                                                                                                           gCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCt                     >  1:4103159/1‑149 (MQ=255)
                                                                                                                              ttCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGtt                  <  1:3604370/149‑1 (MQ=255)
                                                                                                                              ttCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGtt                  >  2:3128217/1‑149 (MQ=255)
                                                                                                                              ttCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGtt                  <  2:2357923/149‑1 (MQ=255)
                                                                                                                                cGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGa                <  2:372817/149‑1 (MQ=255)
                                                                                                                                cGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGa                <  1:3713241/149‑1 (MQ=255)
                                                                                                                                 gATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGAt               <  1:466971/149‑1 (MQ=255)
                                                                                                                                  aTACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATg              >  2:410327/1‑149 (MQ=255)
                                                                                                                                   tACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGc             >  2:302030/1‑149 (MQ=255)
                                                                                                                                      tttGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTg          >  1:3884773/1‑149 (MQ=255)
                                                                                                                                              agaTTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATg  >  1:3390681/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATG  >  CP009273/207867‑208157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑