Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,101,912 T→G 100% E385A (GAG→GCG)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,101,9120TG98.0% 943.2 / 0.7 243E385A (GAG→GCG) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base G (114/124);  total (116/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 244 total aligned reads displayed.

AACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAG  >  CP009273/2101766‑2102057
                                                                                                                                                  |                                                                                                                                                 
     cACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaa                                                                                                                                            >  2:2315172/1‑149 (MQ=255)
       ccAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaaaa                                                                                                                                          <  1:2558727/149‑1 (MQ=255)
       ccAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaaaa                                                                                                                                          <  2:3009346/149‑1 (MQ=255)
          aacaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAagag                                                                                                                                       <  1:1701009/149‑1 (MQ=255)
                  aGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGc                                                                                                                               >  2:2190057/1‑149 (MQ=255)
                   gAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCg                                                                                                                              >  2:1189989/1‑149 (MQ=255)
                     aCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGa                                                                                                                            >  2:1077154/1‑149 (MQ=255)
                     aCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGa                                                                                                                            <  2:2949641/149‑1 (MQ=255)
                        aacaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaa                                                                                                                         >  2:233555/1‑149 (MQ=255)
                        aacaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaa                                                                                                                         >  1:1995911/1‑149 (MQ=255)
                         acaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaaa                                                                                                                        <  2:1034399/149‑1 (MQ=255)
                         acaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaaa                                                                                                                        <  2:3531926/149‑1 (MQ=255)
                         acaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaaa                                                                                                                        >  2:3923047/1‑149 (MQ=255)
                         acaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCATACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaaaa                                                                                                                        >  1:2060990/1‑149 (MQ=255)
                           aaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATc                                                                                                                      >  2:3857495/1‑149 (MQ=255)
                            aTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATca                                                                                                                     >  2:1525532/1‑149 (MQ=255)
                               atatAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaaca                                                                                                                  >  2:1723030/1‑149 (MQ=255)
                                tataATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaa                                                                                                                 <  2:1860139/149‑1 (MQ=255)
                                  taATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaaca                                                                                                               >  1:3255993/1‑149 (MQ=255)
                                   aaTCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaacaa                                                                                                              >  1:108887/1‑149 (MQ=255)
                                   aaTCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaacaa                                                                                                              >  1:2694043/1‑149 (MQ=255)
                                   aaTCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaacaa                                                                                                              <  1:457445/149‑1 (MQ=255)
                                     tCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAAc                                                                                                            <  1:3575332/149‑1 (MQ=255)
                                        tACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTca                                                                                                         <  1:657223/149‑1 (MQ=255)
                                          cATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaac                                                                                                       <  1:678516/149‑1 (MQ=255)
                                            tAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaacaa                                                                                                     >  2:2304480/1‑149 (MQ=255)
                                              aaaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                   >  1:1004842/1‑149 (MQ=255)
                                                 tCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCg                                                                                                >  1:3810655/1‑149 (MQ=255)
                                                     cAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGc                                                                                            <  1:3494147/149‑1 (MQ=255)
                                                       gCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGa                                                                                          <  1:2804187/149‑1 (MQ=255)
                                                        cAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAt                                                                                         <  2:181659/149‑1 (MQ=255)
                                                         aaaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAtt                                                                                        >  2:1290211/1‑149 (MQ=255)
                                                          aaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTa                                                                                       >  1:3144158/1‑149 (MQ=255)
                                                            ccAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaa                                                                                     >  1:295690/1‑149 (MQ=255)
                                                             cAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaaa                                                                                    <  2:134925/149‑1 (MQ=255)
                                                             cAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaaa                                                                                    <  1:1492440/149‑1 (MQ=255)
                                                              aGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAt                                                                                   >  2:164072/1‑149 (MQ=255)
                                                              aGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAt                                                                                   <  1:872030/149‑1 (MQ=255)
                                                               gTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATc                                                                                  <  1:520044/149‑1 (MQ=255)
                                                                tAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCt                                                                                 <  1:3717985/149‑1 (MQ=255)
                                                                 aaTTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCtt                                                                                <  2:622576/149‑1 (MQ=255)
                                                                  aTTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTa                                                                               >  2:2717850/1‑149 (MQ=255)
                                                                   tttATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTAc                                                                              <  1:520642/149‑1 (MQ=255)
                                                                       ttattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGaa                                                                          >  2:4026783/1‑149 (MQ=255)
                                                                        tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAt                                                                         <  2:2282987/149‑1 (MQ=255)
                                                                        tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAt                                                                         >  1:3655753/1‑149 (MQ=255)
                                                                        tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAt                                                                         >  1:481567/1‑149 (MQ=255)
                                                                          tttCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttc                                                                       >  1:2885625/1‑149 (MQ=255)
                                                                           ttCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttct                                                                      <  1:760887/149‑1 (MQ=255)
                                                                           ttCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttct                                                                      >  1:3527064/1‑149 (MQ=255)
                                                                           ttCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttct                                                                      >  1:2906183/1‑149 (MQ=255)
                                                                               tACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctttct                                                                  <  2:1968047/149‑1 (MQ=255)
                                                                               tACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTACACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctttct                                                                  >  1:1825432/1‑149 (MQ=255)
                                                                                 cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTta                                                                <  2:2178314/149‑1 (MQ=255)
                                                                                 cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTta                                                                <  2:1093322/149‑1 (MQ=255)
                                                                                   aaCATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTtata                                                              <  1:1077154/149‑1 (MQ=255)
                                                                                    aCATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATaa                                                             >  2:3454345/1‑149 (MQ=255)
                                                                                    aCATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATaa                                                             >  1:3182407/1‑149 (MQ=255)
                                                                                      aTCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                           <  2:3641737/149‑1 (MQ=255)
                                                                                      aTCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                           >  1:2128441/1‑149 (MQ=255)
                                                                                        cAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                         >  2:912824/1‑149 (MQ=255)
                                                                                         aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATg                                                        <  1:1459673/149‑1 (MQ=255)
                                                                                         aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATg                                                        <  2:2383157/149‑1 (MQ=255)
                                                                                          gCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATgg                                                       >  1:2168393/1‑149 (MQ=255)
                                                                                           cATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATggg                                                      <  1:4052353/149‑1 (MQ=255)
                                                                                           cATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATggg                                                      <  2:3278728/149‑1 (MQ=255)
                                                                                             tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTc                                                    <  1:945306/149‑1 (MQ=255)
                                                                                                gTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGc                                                 >  1:2421719/1‑149 (MQ=255)
                                                                                                 tGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCa                                                <  2:2594260/149‑1 (MQ=255)
                                                                                                     gTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAAc                                            >  2:3388492/1‑149 (MQ=255)
                                                                                                     gTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAAc                                            <  2:2416459/149‑1 (MQ=255)
                                                                                                      tAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACt                                           >  2:3415544/1‑149 (MQ=255)
                                                                                                       aaCTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACtt                                          <  1:1332612/149‑1 (MQ=255)
                                                                                                          tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                       <  1:233555/149‑1 (MQ=255)
                                                                                                          tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                       >  2:1663370/1‑149 (MQ=255)
                                                                                                          tAAGCACTCTGTAGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGATAGCGCAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGaa                                       <  2:3565066/149‑1 (MQ=255)
                                                                                                           aaGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAt                                      <  2:3812757/149‑1 (MQ=255)
                                                                                                                ctctGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCAt                                 >  2:1907330/1‑149 (MQ=255)
                                                                                                                  ctGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAg                               <  1:2667151/149‑1 (MQ=255)
                                                                                                                    gTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAc                             <  2:3884094/149‑1 (MQ=255)
                                                                                                                      tGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAt                           <  1:3804478/149‑1 (MQ=255)
                                                                                                                      tGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACAt                           <  1:1329376/149‑1 (MQ=255)
                                                                                                                        caagcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTa                         <  2:905059/149‑1 (MQ=255)
                                                                                                                           gcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACtt                      >  2:542929/1‑149 (MQ=255)
                                                                                                                           gcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACtt                      <  2:516539/149‑1 (MQ=255)
                                                                                                                           gcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACtt                      <  2:516441/149‑1 (MQ=255)
                                                                                                                            caaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTa                     <  2:3334447/149‑1 (MQ=255)
                                                                                                                              aTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAAt                   >  1:424496/1‑149 (MQ=255)
                                                                                                                              aTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAAt                   >  2:1543148/1‑149 (MQ=255)
                                                                                                                               tGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATg                  <  1:535004/149‑1 (MQ=255)
                                                                                                                                gTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGa                 >  2:2298938/1‑149 (MQ=255)
                                                                                                                                gTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGa                 >  1:305656/1‑149 (MQ=255)
                                                                                                                                 ttATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGaa                <  2:660585/149‑1 (MQ=255)
                                                                                                                                   aTTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAAt              <  1:3158444/149‑1 (MQ=255)
                                                                                                                                   aTTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAAt              <  1:2304480/149‑1 (MQ=255)
                                                                                                                                          cACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaaa       <  2:3144158/149‑1 (MQ=255)
                                                                                                                                            ccAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAgg     >  2:2550052/1‑149 (MQ=255)
                                                                                                                                             cAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGc    >  1:1719253/1‑149 (MQ=255)
                                                                                                                                             cAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGc    <  2:2906183/149‑1 (MQ=255)
                                                                                                                                               aTCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCag  >  1:261023/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAG  >  CP009273/2101766‑2102057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑