Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,110,346 G→A 100% T88I (ACC→ATC)  wcaL ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,110,3460GA99.5% 645.7 / ‑4.4 193T88I (ACC→ATC) wcaLputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (94/98);  total (95/98)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

GGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCG  >  CP009273/2110200‑2110489
                                                                                                                                                  |                                                                                                                                               
   gTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAttt                                                                                                                                            <  2:3497108/149‑1 (MQ=255)
   gTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAttt                                                                                                                                            >  2:3455871/1‑149 (MQ=255)
      ttGGTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTa                                                                                                                                         <  2:901525/149‑1 (MQ=255)
       tgctgcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTAc                                                                                                                                        <  1:3758270/149‑1 (MQ=255)
         ctgcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGa                                                                                                                                      <  1:737614/149‑1 (MQ=255)
                 aCCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGcc                                                                                                                              <  1:3804319/149‑1 (MQ=255)
                 aCCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGcc                                                                                                                              >  2:1611762/1‑149 (MQ=255)
                         gggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAgg                                                                                                                      >  1:2704444/1‑149 (MQ=255)
                         gggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAgg                                                                                                                      <  2:1740237/149‑1 (MQ=255)
                         gggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAgg                                                                                                                      <  2:848696/149‑1 (MQ=255)
                          ggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGt                                                                                                                     >  2:1553281/1‑149 (MQ=255)
                          ggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGt                                                                                                                     <  1:489732/149‑1 (MQ=255)
                          ggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGt                                                                                                                     >  2:3386507/1‑149 (MQ=255)
                           gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTc                                                                                                                    <  2:264504/149‑1 (MQ=255)
                           gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTc                                                                                                                    >  2:3343340/1‑149 (MQ=255)
                           gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGAAATTTTTACGATGAATGCCGCGCAAGGTc                                                                                                                    >  2:1715791/1‑149 (MQ=255)
                            aCCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTct                                                                                                                   >  1:896649/1‑149 (MQ=255)
                               aaaaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggc                                                                                                                <  1:2377486/149‑1 (MQ=255)
                               aaaaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCGGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggc                                                                                                                <  1:740572/149‑1 (MQ=255)
                                aaaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggct                                                                                                               >  1:3881987/1‑149 (MQ=255)
                                  aTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggctgg                                                                                                             >  1:2991758/1‑149 (MQ=255)
                                   tGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCtggctggc                                                                                                            >  2:3877029/1‑149 (MQ=255)
                                        gATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGt                                                                                                       <  2:1832451/149‑1 (MQ=255)
                                          tGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTgg                                                                                                     >  1:3117912/1‑149 (MQ=255)
                                           gAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTct                                                                                                                   <  2:2261872/134‑1 (MQ=255)
                                           gAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTct                                                                                                                   >  1:2261872/1‑134 (MQ=255)
                                           gAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTct                                                                                                                   >  1:186511/1‑134 (MQ=255)
                                           gAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGgc                                                                                                    >  2:1625085/1‑149 (MQ=255)
                                             acacATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGgcgc                                                                                                  >  1:1592221/1‑149 (MQ=255)
                                                caTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCACGGTCTGGCTGGCTCGGTGGCGCAGt                                                                                               >  1:944714/1‑149 (MQ=255)
                                                caTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGAGCAGt                                                                                               >  2:426921/1‑149 (MQ=255)
                                                  tCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGttt                                                                                             >  2:803197/1‑149 (MQ=255)
                                                  tCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGGTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGttt                                                                                             <  2:2485206/149‑1 (MQ=255)
                                                   cGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTc                                                                                            <  1:1942616/149‑1 (MQ=255)
                                                    ggCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCg                                                                                           >  1:1440198/1‑149 (MQ=255)
                                                        cgAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCAc                                                                                       <  1:3942537/149‑1 (MQ=255)
                                                        cgAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCAc                                                                                       <  2:3714443/149‑1 (MQ=255)
                                                         gAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACt                                                                                      <  2:620363/149‑1 (MQ=255)
                                                         gAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACt                                                                                      >  2:3423980/1‑149 (MQ=255)
                                                          aaaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACtt                                                                                     >  2:3285546/1‑149 (MQ=255)
                                                            aCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTg                                                                                   >  2:2850185/1‑149 (MQ=255)
                                                             cGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGc                                                                                  <  2:2793802/149‑1 (MQ=255)
                                                              ggTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGcc                                                                                 >  2:3121669/1‑149 (MQ=255)
                                                                  ttGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTa                                                                             <  2:2404643/149‑1 (MQ=255)
                                                                  ttGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTa                                                                             <  2:861204/149‑1 (MQ=255)
                                                                      gACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGtt                                                                         <  1:3646485/149‑1 (MQ=255)
                                                                       aCCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTc                                                                        >  1:1135380/1‑149 (MQ=255)
                                                                          tGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTc                                                                     <  1:3455871/149‑1 (MQ=255)
                                                                          tGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTc                                                                     <  1:2315917/149‑1 (MQ=255)
                                                                           ggCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTcc                                                                    <  1:3044314/149‑1 (MQ=255)
                                                                            gCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCt                                                                   >  1:1799320/1‑149 (MQ=255)
                                                                                 aaaTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                                              >  2:2676944/1‑149 (MQ=255)
                                                                                    tGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAg                                                           <  2:2920632/149‑1 (MQ=255)
                                                                                      gCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg                                                         >  2:3648309/1‑149 (MQ=255)
                                                                                      gCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg                                                         <  2:2327490/149‑1 (MQ=255)
                                                                                         gACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGt                                                      <  1:1468467/149‑1 (MQ=255)
                                                                                          aCAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGtt                                                     <  2:3566991/149‑1 (MQ=255)
                                                                                           cAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTc                                                    <  1:708937/149‑1 (MQ=255)
                                                                                            aaaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCt                                                   >  1:3246114/1‑149 (MQ=255)
                                                                                            aaaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCt                                                   >  1:2038770/1‑149 (MQ=255)
                                                                                            aaaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCt                                                   >  1:2954804/1‑149 (MQ=255)
                                                                                               aTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGgc                                                >  1:1236027/1‑149 (MQ=255)
                                                                                                tCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGgca                                               >  1:253894/1‑149 (MQ=255)
                                                                                                    gTTCCGCGACTCGGCACCATAGCGTGTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCaa                                           <  2:2331582/149‑1 (MQ=255)
                                                                                                       ccGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGt                                        >  1:4002605/1‑149 (MQ=255)
                                                                                                         gcgACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg                                      >  1:827637/1‑149 (MQ=255)
                                                                                                            aCTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAt                                   >  1:3082570/1‑149 (MQ=255)
                                                                                                             cTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAtt                                  <  1:4051242/149‑1 (MQ=255)
                                                                                                              tCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAttt                                 <  2:1033839/149‑1 (MQ=255)
                                                                                                              tCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAttt                                 <  2:3090651/149‑1 (MQ=255)
                                                                                                              tCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAttt                                 >  2:3582847/1‑149 (MQ=255)
                                                                                                              tCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAttt                                 >  2:1316791/1‑149 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCa                           <  1:3410042/149‑1 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCa                           >  1:2282527/1‑149 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCa                           <  1:3121683/149‑1 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCa                           <  1:1363869/149‑1 (MQ=255)
                                                                                                                      aTAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATg                         >  2:2900904/1‑149 (MQ=255)
                                                                                                                       tAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGc                        <  1:1025371/149‑1 (MQ=255)
                                                                                                                       tAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGc                        >  1:2182410/1‑149 (MQ=255)
                                                                                                                       tAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGc                        <  2:3043464/149‑1 (MQ=255)
                                                                                                                       tAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGc                        <  2:775464/149‑1 (MQ=255)
                                                                                                                       tAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGc                        <  1:885554/149‑1 (MQ=255)
                                                                                                                       tAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTGTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGc                        <  2:2833779/149‑1 (MQ=255)
                                                                                                                        aGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGTGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGcc                       >  1:3365330/1‑149 (MQ=255)
                                                                                                                         gCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGAGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCcg                      >  2:1915221/1‑149 (MQ=255)
                                                                                                                            tttGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGt                   >  2:255667/1‑149 (MQ=255)
                                                                                                                             ttGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCACGGTTGTAGTTCGTCCATGCcgcggg                  >  2:2969482/1‑147 (MQ=255)
                                                                                                                              tGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTgg                 <  2:844998/149‑1 (MQ=255)
                                                                                                                               gAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTggg                <  1:370707/149‑1 (MQ=255)
                                                                                                                               gAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTggg                <  1:231448/149‑1 (MQ=255)
                                                                                                                                aGGTTGAGCGCCTGCCAGATATTTTTACGATGCATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGGAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGGATTTCGTCCATGCCGCGTgggg               >  2:3667337/1‑148 (MQ=255)
                                                                                                                                   ttGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtgtg            >  1:2605564/1‑148 (MQ=255)
                                                                                                                                   ttGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGtt            <  2:730209/149‑1 (MQ=255)
                                                                                                                                    tGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGttt           <  1:1079005/149‑1 (MQ=255)
                                                                                                                                     gAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGtttt          >  1:1682677/1‑149 (MQ=255)
                                                                                                                                     gAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGtttt          <  1:3485323/149‑1 (MQ=255)
                                                                                                                                     gAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGtttt          >  1:2628245/1‑149 (MQ=255)
                                                                                                                                       gcgcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGAGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgt        <  1:344554/149‑1 (MQ=255)
                                                                                                                                         gcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtgt      <  2:2168204/149‑1 (MQ=255)
                                                                                                                                            tGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTc   <  1:1715791/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCG  >  CP009273/2110200‑2110489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑