Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,122,013 G→T 100% T119T (ACC→ACA)  wcaF ← putative acyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,122,0130GT98.8% 781.4 / 0.4 240T119T (ACC→ACA) wcaFputative acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (98/139);  total (98/142)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

CCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAT  >  CP009273/2121865‑2122157
                                                                                                                                                    |                                                                                                                                                
    gCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGa                                                                                                                                              >  1:1559619/1‑149 (MQ=255)
      aaaCCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGaaa                                                                                                                                            >  2:548234/1‑149 (MQ=255)
       aaCCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAAt                                                                                                                                           <  2:1188649/149‑1 (MQ=255)
        aCCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATg                                                                                                                                          >  1:811693/1‑149 (MQ=255)
         ccACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGt                                                                                                                                         <  2:1296323/149‑1 (MQ=255)
         ccACATGTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGt                                                                                                                                         <  2:3348395/149‑1 (MQ=255)
           acaTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTg                                                                                                                                       >  1:3781063/1‑149 (MQ=255)
            caTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGa                                                                                                                                      >  2:2514604/1‑149 (MQ=255)
             aTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGAc                                                                                                                                     >  1:1760316/1‑149 (MQ=255)
               ttGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACtt                                                                                                                                   <  1:2979282/149‑1 (MQ=255)
               ttGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACtt                                                                                                                                   <  2:2339207/149‑1 (MQ=255)
                  ccGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCa                                                                                                                                <  1:3167376/149‑1 (MQ=255)
                    ggAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGTTTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATg                                                                                                                              >  1:672385/1‑149 (MQ=255)
                    ggAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATg                                                                                                                              >  1:672992/1‑149 (MQ=255)
                    ggAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATg                                                                                                                              >  1:3293155/1‑149 (MQ=255)
                        gCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCg                                                                                                                          <  2:4027317/149‑1 (MQ=255)
                         cGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGt                                                                                                                         <  1:3648179/149‑1 (MQ=255)
                           aTTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTgg                                                                                                                       >  1:2143791/1‑149 (MQ=255)
                            tttAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGc                                                                                                                      >  1:3304958/1‑149 (MQ=255)
                              tAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTa                                                                                                                    >  1:3881306/1‑149 (MQ=255)
                                aaaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTAcc                                                                                                                  <  1:2469041/149‑1 (MQ=255)
                                   acacTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGt                                                                                                               >  2:1306674/1‑149 (MQ=255)
                                   acacTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGt                                                                                                               <  2:3515961/149‑1 (MQ=255)
                                      cTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCa                                                                                                            <  2:1329293/149‑1 (MQ=255)
                                        gCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCata                                                                                                          <  1:1175773/149‑1 (MQ=255)
                                          ttCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaa                                                                                                        >  1:3507779/1‑149 (MQ=255)
                                           tCGTGCACCCCCGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGGTGCCAGCCAGCATGTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaat                                                                                                       <  2:2444049/149‑1 (MQ=255)
                                           tCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaat                                                                                                       <  2:374460/149‑1 (MQ=255)
                                            cGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaata                                                                                                      <  1:106027/149‑1 (MQ=255)
                                             gTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaataa                                                                                                     <  2:272791/149‑1 (MQ=255)
                                             gTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaataa                                                                                                     >  1:3998587/1‑149 (MQ=255)
                                              tGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAAc                                                                                                    <  1:886484/149‑1 (MQ=255)
                                                cACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACtt                                                                                                  <  1:1399862/149‑1 (MQ=255)
                                                   ccACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACttttt                                                                                               >  2:3064979/1‑149 (MQ=255)
                                                    cACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACtttttt                                                                                              <  2:496066/149‑1 (MQ=255)
                                                    cACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACtttttt                                                                                              <  1:285150/149‑1 (MQ=255)
                                                     acgacgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTg                                                                                             <  2:1495158/149‑1 (MQ=255)
                                                      cgacgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGc                                                                                            <  2:1559619/149‑1 (MQ=255)
                                                       gacgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCg                                                                                           >  2:198004/1‑149 (MQ=255)
                                                        acgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAGAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGa                                                                                          <  2:1048073/149‑1 (MQ=255)
                                                        acgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGa                                                                                          <  1:573597/149‑1 (MQ=255)
                                                         cgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGat                                                                                         <  1:1861154/149‑1 (MQ=255)
                                                           gTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGatat                                                                                       >  1:560213/1‑149 (MQ=255)
                                                               cGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCAcc                                                                                   <  1:2380459/149‑1 (MQ=255)
                                                               cGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCAcc                                                                                   <  1:447038/149‑1 (MQ=255)
                                                               cGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCAcc                                                                                   <  1:2380310/149‑1 (MQ=255)
                                                                 tCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGa                                                                                 >  2:1420674/1‑149 (MQ=255)
                                                                 tCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGa                                                                                 <  2:1178018/149‑1 (MQ=255)
                                                                  cGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGaa                                                                                <  1:145150/149‑1 (MQ=255)
                                                                            gACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCaa                                                                      >  2:889750/1‑149 (MQ=255)
                                                                                   ggggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAt                                                               >  1:2666039/1‑149 (MQ=255)
                                                                                   ggggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAt                                                               <  2:1621621/149‑1 (MQ=255)
                                                                                     ggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAttt                                                             <  1:3365803/149‑1 (MQ=255)
                                                                                      gCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTc                                                            <  2:1432364/149‑1 (MQ=255)
                                                                                        aacaaAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCAc                                                          >  2:2298275/1‑149 (MQ=255)
                                                                                        aacaaAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCAc                                                          >  1:677438/1‑149 (MQ=255)
                                                                                         acaaAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTNGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCAcc                                                         >  1:2080652/1‑149 (MQ=255)
                                                                                          caaAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCg                                                        <  2:138865/149‑1 (MQ=255)
                                                                                           aaaGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGTTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGa                                                       >  2:178859/1‑149 (MQ=255)
                                                                                             aGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAgg                                                     >  2:3031665/1‑149 (MQ=255)
                                                                                                   cGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtatat                                               <  1:1092894/149‑1 (MQ=255)
                                                                                                    ggTTGACAGCCAGCATTTCTCGCCAATCACAAAAGTCGTGGCGTTAATTTTGAAATGATGACTTGCATGGTCGTGGCTAACCGTGCATAACTAACTTTTTTGCGATATCACCTAATGTGCGCCAATGGTAATATCACCGAGGGtatata                                              >  1:2133438/1‑149 (MQ=255)
                                                                                                     gTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATaa                                             >  2:917897/1‑149 (MQ=255)
                                                                                                     gTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATaa                                             >  2:2827069/1‑149 (MQ=255)
                                                                                                      ttGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATaaa                                            <  1:3963591/149‑1 (MQ=255)
                                                                                                        gccagccaGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAtt                                          >  2:1111961/1‑149 (MQ=255)
                                                                                                         ccagccagCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTg                                         <  1:3296926/149‑1 (MQ=255)
                                                                                                          cagccagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGa                                        <  2:3720107/149‑1 (MQ=255)
                                                                                                           agccagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGAc                                       >  1:277842/1‑149 (MQ=255)
                                                                                                           agccagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGAc                                       <  1:3017224/149‑1 (MQ=255)
                                                                                                           agccagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGAc                                       <  2:168987/149‑1 (MQ=255)
                                                                                                            gccagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACg                                      >  1:1803094/1‑149 (MQ=255)
                                                                                                               agcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCa                                   <  1:110254/149‑1 (MQ=255)
                                                                                                                gcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  <  1:361481/149‑1 (MQ=255)
                                                                                                                gcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  <  1:918172/149‑1 (MQ=255)
                                                                                                                gcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  <  1:478269/149‑1 (MQ=255)
                                                                                                                gcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  >  2:3293484/1‑149 (MQ=255)
                                                                                                                gcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  <  2:2723991/149‑1 (MQ=255)
                                                                                                                gcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  >  2:1939030/1‑149 (MQ=255)
                                                                                                                 cATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATc                                 <  1:1790206/149‑1 (MQ=255)
                                                                                                                       tcGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGAc                           <  1:3681612/149‑1 (MQ=255)
                                                                                                                       tcGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGAc                           >  1:2733598/1‑149 (MQ=255)
                                                                                                                         gCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACGGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGAccc                         <  2:1548319/149‑1 (MQ=255)
                                                                                                                          ccAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCa                        >  1:2809956/1‑149 (MQ=255)
                                                                                                                             aTCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAcgc                     >  2:3436767/1‑149 (MQ=255)
                                                                                                                              tCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAcgcg                    >  2:2135491/1‑149 (MQ=255)
                                                                                                                                acaATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTa                  <  1:447796/149‑1 (MQ=255)
                                                                                                                                    tAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCa              >  1:2589611/1‑149 (MQ=255)
                                                                                                                                     aGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAc             <  2:16809/149‑1 (MQ=255)
                                                                                                                                      ggcgtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAcc            <  1:1690166/149‑1 (MQ=255)
                                                                                                                                      ggcgtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAcc            >  2:49364/1‑149 (MQ=255)
                                                                                                                                      ggcgtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAcc            <  1:1690211/149‑1 (MQ=255)
                                                                                                                                       gcgtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCt           <  2:699656/149‑1 (MQ=255)
                                                                                                                                          tggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAg        <  2:2007850/149‑1 (MQ=255)
                                                                                                                                            gcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGt      <  1:1454211/149‑1 (MQ=255)
                                                                                                                                            gcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGt      >  2:1823537/1‑149 (MQ=255)
                                                                                                                                            gcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGt      <  1:1979784/149‑1 (MQ=255)
                                                                                                                                              gtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTa    <  1:2923302/149‑1 (MQ=255)
                                                                                                                                               ttAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTaa   <  1:3862929/149‑1 (MQ=255)
                                                                                                                                                tAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAt  <  2:350835/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAT  >  CP009273/2121865‑2122157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑