Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,152,444 C→A 100% A193E (GCG→GAG)  mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,152,4440CA98.7% 744.4 / 0.5 227A193E (GCG→GAG) mdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (111/113);  total (113/114)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

GACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATAT  >  CP009273/2152298‑2152589
                                                                                                                                                  |                                                                                                                                                 
gACGTCCGATACTTATTCGCAGGGTGAACTGTCCGATTTCGCCTCGACGCAGCTGGCTCCGACGATGTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGgagc                                                                                                                                                 <  2:221130/149‑1 (MQ=255)
gACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGgagc                                                                                                                                                 >  2:3621821/1‑149 (MQ=255)
gACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGgagc                                                                                                                                                 >  1:802464/1‑149 (MQ=255)
 aCGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCt                                                                                                                                                <  1:2647704/149‑1 (MQ=255)
 aCGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCt                                                                                                                                                <  2:3432604/149‑1 (MQ=255)
 aCGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCt                                                                                                                                                >  2:3184829/1‑149 (MQ=255)
     ccGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAACTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGttt                                                                                                                                            <  1:3451350/149‑1 (MQ=255)
        aTACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAAt                                                                                                                                         >  1:394103/1‑149 (MQ=255)
        aTACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAAt                                                                                                                                         <  1:2315469/149‑1 (MQ=255)
         tACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATc                                                                                                                                        >  2:1458873/1‑149 (MQ=255)
          aCTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCa                                                                                                                                       >  1:1176988/1‑149 (MQ=255)
          aCTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCa                                                                                                                                       >  2:107136/1‑149 (MQ=255)
           cTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAg                                                                                                                                      >  1:3193785/1‑149 (MQ=255)
            ttATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAgg                                                                                                                                     >  1:2576528/1‑149 (MQ=255)
            ttATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAgg                                                                                                                                     >  2:3820610/1‑149 (MQ=255)
             tATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAggg                                                                                                                                    >  2:815756/1‑149 (MQ=255)
              aTTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGc                                                                                                                                   >  2:1766570/1‑149 (MQ=255)
               ttCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCg                                                                                                                                  >  2:1873830/1‑149 (MQ=255)
                 cGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCgtg                                                                                                                                >  1:2966341/1‑149 (MQ=255)
                  gCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCgtgt                                                                                                                               <  2:3609854/149‑1 (MQ=255)
                   cAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTc                                                                                                                              <  1:2674119/149‑1 (MQ=255)
                     gggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGc                                                                                                                            <  1:994178/149‑1 (MQ=255)
                     gggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGc                                                                                                                            <  1:2376953/149‑1 (MQ=255)
                      ggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCt                                                                                                                           <  1:547730/149‑1 (MQ=255)
                      ggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCt                                                                                                                           <  1:94257/149‑1 (MQ=255)
                      ggTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCt                                                                                                                           <  1:3176769/149‑1 (MQ=255)
                         gAACTGTACGATTTCGCCTCGACGCAGCTGGCACCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGga                                                                                                                        <  1:1562745/149‑1 (MQ=255)
                             tGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGgacgac                                                                                                                    <  1:2864754/149‑1 (MQ=255)
                                aCGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTa                                                                                                                 <  1:3576484/149‑1 (MQ=255)
                                  gATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACg                                                                                                               >  1:2512972/1‑149 (MQ=255)
                                  gATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACg                                                                                                               >  1:1835844/1‑149 (MQ=255)
                                   aTTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGc                                                                                                              >  2:3602184/1‑149 (MQ=255)
                                    tttCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCa                                                                                                             >  2:2350628/1‑149 (MQ=255)
                                    tttCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCa                                                                                                             >  1:2877750/1‑149 (MQ=255)
                                    tttCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCa                                                                                                             >  1:3555260/1‑149 (MQ=255)
                                        gCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAccgc                                                                                                         >  1:3474155/1‑149 (MQ=255)
                                            cGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTc                                                                                                     <  2:1427067/149‑1 (MQ=255)
                                             gACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCa                                                                                                    <  1:3210389/149‑1 (MQ=255)
                                              aCGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAg                                                                                                   >  1:419837/1‑149 (MQ=255)
                                                gCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCa                                                                                                 >  2:3415957/1‑149 (MQ=255)
                                                 cAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCaa                                                                                                <  1:480322/149‑1 (MQ=255)
                                                    cTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGc                                                                                             <  1:1331870/149‑1 (MQ=255)
                                                     tGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGcc                                                                                            <  2:3229655/149‑1 (MQ=255)
                                                       gCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCaa                                                                                          <  1:2416492/149‑1 (MQ=255)
                                                         tCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACg                                                                                        >  2:3093173/1‑149 (MQ=255)
                                                            gacgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGc                                                                                     <  2:2546879/149‑1 (MQ=255)
                                                            gacgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGc                                                                                     >  2:803238/1‑149 (MQ=255)
                                                                 tttCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTaaa                                                                                <  2:44534/149‑1 (MQ=255)
                                                                 tttCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTaaa                                                                                <  2:19638/149‑1 (MQ=255)
                                                                 tttCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTaaa                                                                                <  1:3877826/149‑1 (MQ=255)
                                                                 tttCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTaaa                                                                                >  2:661924/1‑149 (MQ=255)
                                                                  ttCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAAc                                                                               >  1:520037/1‑149 (MQ=255)
                                                                      cAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCa                                                                           <  1:1436261/149‑1 (MQ=255)
                                                                        aaTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAgg                                                                         >  2:1639447/1‑149 (MQ=255)
                                                                            gACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTACGTAAACCGCAGGgcgc                                                                     >  2:1738916/1‑149 (MQ=255)
                                                                              cGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGgcgcgc                                                                   >  1:2049351/1‑149 (MQ=255)
                                                                                gtgtTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTg                                                                 >  1:3038333/1‑149 (MQ=255)
                                                                                   ttGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGaa                                                              <  1:3291744/149‑1 (MQ=255)
                                                                                    tGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAg                                                             >  2:1190582/1‑149 (MQ=255)
                                                                                     ggTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGa                                                            >  2:424950/1‑149 (MQ=255)
                                                                                      gTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGAt                                                           <  2:1599379/149‑1 (MQ=255)
                                                                                       tGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATg                                                          <  2:4078143/149‑1 (MQ=255)
                                                                                       tGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATg                                                          >  2:2772708/1‑149 (MQ=255)
                                                                                       tGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATg                                                          <  2:2835534/149‑1 (MQ=255)
                                                                                        gatgtcgatgtcGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATgg                                                         <  2:604505/149‑1 (MQ=255)
                                                                                        gatgtcgatgtcGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATgg                                                         >  2:391767/1‑149 (MQ=255)
                                                                                        gatgtcgatgtcGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATgg                                                         >  2:3537895/1‑149 (MQ=255)
                                                                                          tgtcgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCAGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGAAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCa                                                       >  1:530260/1‑149 (MQ=255)
                                                                                           gtcgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCAc                                                      >  1:2105656/1‑149 (MQ=255)
                                                                                           gtcgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCAc                                                      >  1:694106/1‑149 (MQ=255)
                                                                                              gatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCa                                                   <  1:3416583/149‑1 (MQ=255)
                                                                                               atgtcgGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCAc                                                  >  1:418772/1‑149 (MQ=255)
                                                                                                    ggAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTg                                             <  1:107136/149‑1 (MQ=255)
                                                                                                          aGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGAt                                       <  2:2615547/149‑1 (MQ=255)
                                                                                                            cTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATcc                                     <  2:888429/149‑1 (MQ=255)
                                                                                                             tCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCCGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCa                                    >  1:3125148/1‑149 (MQ=255)
                                                                                                             tCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCa                                    <  2:232561/149‑1 (MQ=255)
                                                                                                             tCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCa                                    <  1:898983/149‑1 (MQ=255)
                                                                                                               aCTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGa                                  <  2:1562158/149‑1 (MQ=255)
                                                                                                                cTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGAc                                 <  2:2036256/149‑1 (MQ=255)
                                                                                                                cTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGAc                                 <  2:2056280/149‑1 (MQ=255)
                                                                                                                    ccgccgTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAat                             <  1:2758030/149‑1 (MQ=255)
                                                                                                                      gccgTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCATCGCTGGCAGATCCAGACCAatga                           <  2:3193756/149‑1 (MQ=255)
                                                                                                                      gccgTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAatga                           <  2:3014844/149‑1 (MQ=255)
                                                                                                                       ccgTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAatgat                          <  2:293581/149‑1 (MQ=255)
                                                                                                                        cgTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAatgatg                         <  1:2584462/149‑1 (MQ=255)
                                                                                                                         gTACGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAAtgatga                        >  2:2639551/1‑149 (MQ=255)
                                                                                                                           aCGCGTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGc                      <  1:8595/149‑1 (MQ=255)
                                                                                                                              cgTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTaa                   <  2:1351155/149‑1 (MQ=255)
                                                                                                                               gTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTaaa                  >  2:3517810/1‑149 (MQ=255)
                                                                                                                               gTCGGGCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTaaa                  >  2:2663290/1‑149 (MQ=255)
                                                                                                                                  gggCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAAc               <  2:492855/149‑1 (MQ=255)
                                                                                                                                    gCTGAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAAccg             >  1:2553166/1‑149 (MQ=255)
                                                                                                                                       gAATCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAAccgccg          >  1:2954033/1‑149 (MQ=255)
                                                                                                                                         aTCCGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCt        <  2:2848325/149‑1 (MQ=255)
                                                                                                                                           ccGCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGa      >  1:1681817/1‑149 (MQ=255)
                                                                                                                                             gCAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAat    >  2:2247134/1‑149 (MQ=255)
                                                                                                                                              cAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAata   <  2:520037/149‑1 (MQ=255)
                                                                                                                                              cAGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAata   <  2:733481/149‑1 (MQ=255)
                                                                                                                                               aGGAGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAACACCGCCGCTGAatat  <  1:2164909/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GACGTCCGATACTTATTCGCAGGGTGAACTGTACGATTTCGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAACGTGCGTAAACCGCAGGGCGCGCTGGAAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATAT  >  CP009273/2152298‑2152589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑