Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,218,022 C→A 100% E110* (GAA→TAA)  pbpG ← D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,218,0220CA98.7% 771.4 / 0.5 234E110* (GAA→TAA) pbpGD‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (121/110);  total (123/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

GTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCACC  >  CP009273/2217876‑2218167
                                                                                                                                                  |                                                                                                                                                 
gttgttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGc                                                                                                                                                 >  1:4057216/1‑149 (MQ=255)
   gttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTAt                                                                                                                                              <  1:134821/149‑1 (MQ=255)
   gttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTAt                                                                                                                                              <  1:1748702/149‑1 (MQ=255)
       aTTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAg                                                                                                                                          <  2:2736007/149‑1 (MQ=255)
        ttCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGt                                                                                                                                         >  2:77394/1‑149 (MQ=255)
         tCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTc                                                                                                                                        <  1:829163/149‑1 (MQ=255)
            gAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTa                                                                                                                                     >  2:2060761/1‑149 (MQ=255)
                  tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgcg                                                                                                                               >  1:371315/1‑149 (MQ=255)
                        tttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGa                                                                                                                         >  2:2167238/1‑149 (MQ=255)
                        tttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGa                                                                                                                         <  1:2058473/149‑1 (MQ=255)
                            gcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGAcccc                                                                                                                     <  1:990487/149‑1 (MQ=255)
                            gcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGAcccc                                                                                                                     >  2:918214/1‑149 (MQ=255)
                            gcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGAcccc                                                                                                                     <  1:3048273/149‑1 (MQ=255)
                             cattcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCt                                                                                                                    <  1:3636115/149‑1 (MQ=255)
                             cattcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCt                                                                                                                    <  1:3020093/149‑1 (MQ=255)
                              attcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGACGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGAGTTAGCTAGGCAGTCGTACGCGCGAATAGACCCCtt                                                                                                                   >  2:468532/1‑149 (MQ=255)
                              attcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCtt                                                                                                                   >  1:675764/1‑149 (MQ=255)
                               ttcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCttt                                                                                                                  >  2:2884578/1‑149 (MQ=255)
                                 cattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCa                                                                                                                >  2:75885/1‑149 (MQ=255)
                                   ttGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAtc                                                                                                              <  1:1342087/149‑1 (MQ=255)
                                       cTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCg                                                                                                          >  2:1533217/1‑149 (MQ=255)
                                        ttAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCgg                                                                                                         <  2:2529409/149‑1 (MQ=255)
                                        ttAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCgg                                                                                                         >  1:846880/1‑149 (MQ=255)
                                          aataaAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCg                                                                                                       >  2:3252250/1‑149 (MQ=255)
                                           ataaAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGt                                                                                                      >  1:2273075/1‑149 (MQ=255)
                                           ataaAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGt                                                                                                      >  1:2967149/1‑149 (MQ=255)
                                            taaAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTc                                                                                                     >  1:1512185/1‑149 (MQ=255)
                                             aaaGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCt                                                                                                    >  2:1025562/1‑149 (MQ=255)
                                              aaGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATGCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTg                                                                                                   >  2:33883/1‑149 (MQ=255)
                                               aGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGCATAGACCCCTTTCATCTCCGGCGTCTgg                                                                                                  <  2:1631108/149‑1 (MQ=255)
                                               aGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTgg                                                                                                  >  2:2278230/1‑149 (MQ=255)
                                                ggCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGc                                                                                                 >  2:3787851/1‑149 (MQ=255)
                                                ggCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGc                                                                                                 >  1:1898239/1‑149 (MQ=255)
                                                   tttGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGa                                                                                              <  2:2369032/149‑1 (MQ=255)
                                                   tttGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGa                                                                                              >  1:3605043/1‑149 (MQ=255)
                                                    ttGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGat                                                                                             <  1:2470845/149‑1 (MQ=255)
                                                      gTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGatat                                                                                           >  1:1082042/1‑149 (MQ=255)
                                                       tAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATc                                                                                          <  1:273221/149‑1 (MQ=255)
                                                         accaccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCa                                                                                        >  1:3346419/1‑149 (MQ=255)
                                                         accaccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCa                                                                                        >  2:2774604/1‑149 (MQ=255)
                                                          ccaccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCAc                                                                                       >  1:2112855/1‑149 (MQ=255)
                                                          ccaccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCAc                                                                                       <  2:541040/149‑1 (MQ=255)
                                                           caccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACt                                                                                      <  1:1253240/149‑1 (MQ=255)
                                                              cGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACtttt                                                                                   >  1:2825797/1‑149 (MQ=255)
                                                              cGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACtttt                                                                                   <  1:2432908/149‑1 (MQ=255)
                                                               gggATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTa                                                                                  <  2:370240/149‑1 (MQ=255)
                                                               gggATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTa                                                                                  <  2:370143/149‑1 (MQ=255)
                                                                ggATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAg                                                                                 >  2:2437613/1‑149 (MQ=255)
                                                                 gATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGt                                                                                <  2:1344976/149‑1 (MQ=255)
                                                                   tAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGttt                                                                              <  1:2337736/149‑1 (MQ=255)
                                                                       ggTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCa                                                                          <  1:3697081/149‑1 (MQ=255)
                                                                         tGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATc                                                                        >  2:4111352/1‑149 (MQ=255)
                                                                         tGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATc                                                                        <  1:1545108/149‑1 (MQ=255)
                                                                               atgCTAGCCGCCGCGCGGTTTTCTGAAGACAACAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGACTAGACACCTTTCATCTCCTGCGACTGGCTTATATCCACTTTTAGTTTTTAATACAGCgg                                                                  >  2:3161787/3‑149 (MQ=255)
                                                                               aGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCgg                                                                  >  1:2661085/1‑149 (MQ=255)
                                                                               aGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCgg                                                                  >  2:2488792/1‑149 (MQ=255)
                                                                               aGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACGTTTAGTTTTTCATCCAGCgg                                                                  >  2:3044636/1‑149 (MQ=255)
                                                                                ggCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGc                                                                 <  1:1593051/149‑1 (MQ=255)
                                                                                   ttGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGt                                                              >  1:3698474/1‑149 (MQ=255)
                                                                                   ttGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGt                                                              >  1:1652638/1‑149 (MQ=255)
                                                                                    tGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTc                                                             <  1:1800355/149‑1 (MQ=255)
                                                                                      ccgccgCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGt                                                           <  1:4020959/149‑1 (MQ=255)
                                                                                      ccgccgCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGt                                                           >  2:3326594/1‑149 (MQ=255)
                                                                                        gccgcGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGc                                                         >  1:3555888/1‑149 (MQ=255)
                                                                                        gccgcGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGc                                                         >  1:2593048/1‑149 (MQ=255)
                                                                                         ccgcGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCa                                                        <  1:2809292/149‑1 (MQ=255)
                                                                                           gcgcgGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATc                                                      >  2:2555725/1‑149 (MQ=255)
                                                                                             gcgGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCa                                                    >  1:1861131/1‑149 (MQ=255)
                                                                                                gTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGca                                                 >  1:343612/1‑149 (MQ=255)
                                                                                                    tCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAAc                                             <  1:589110/149‑1 (MQ=255)
                                                                                                     cTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAAcc                                            <  1:1591870/149‑1 (MQ=255)
                                                                                                      tGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCa                                           >  2:1587571/1‑149 (MQ=255)
                                                                                                       gAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCAt                                          <  1:3848885/149‑1 (MQ=255)
                                                                                                       gAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCAt                                          >  1:308984/1‑149 (MQ=255)
                                                                                                        aaGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATc                                         <  2:823659/149‑1 (MQ=255)
                                                                                                              tCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTc                                   >  2:1490493/1‑149 (MQ=255)
                                                                                                                 gcgcCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCAtt                                <  2:363659/149‑1 (MQ=255)
                                                                                                                     cAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAAtt                            >  2:1853801/1‑149 (MQ=255)
                                                                                                                     cAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAAtt                            <  2:3499175/149‑1 (MQ=255)
                                                                                                                        cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGc                         <  2:1859485/149‑1 (MQ=255)
                                                                                                                        cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGc                         <  2:1723298/149‑1 (MQ=255)
                                                                                                                        cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGc                         <  1:1334610/149‑1 (MQ=255)
                                                                                                                           cAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGa                      <  2:1082042/149‑1 (MQ=255)
                                                                                                                            aGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGAt                     <  2:3876235/149‑1 (MQ=255)
                                                                                                                               atatCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGa                  <  1:1182202/149‑1 (MQ=255)
                                                                                                                                tatCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGAc                 <  1:2167238/149‑1 (MQ=255)
                                                                                                                                tatCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGAc                 >  2:4101884/1‑149 (MQ=255)
                                                                                                                                 atCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACg                >  2:1234054/1‑149 (MQ=255)
                                                                                                                                  tCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGc               >  2:3856645/1‑149 (MQ=255)
                                                                                                                                    tttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCaa             <  2:2661085/149‑1 (MQ=255)
                                                                                                                                    tttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCaa             >  2:1998436/1‑149 (MQ=255)
                                                                                                                                    tttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCaa             <  2:595869/149‑1 (MQ=255)
                                                                                                                                    tttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCaa             >  1:2917765/1‑149 (MQ=255)
                                                                                                                                      tACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATc           <  1:2910085/149‑1 (MQ=255)
                                                                                                                                      tACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATc           >  1:752241/1‑149 (MQ=255)
                                                                                                                                      tACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCGTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATc           <  2:2303588/149‑1 (MQ=255)
                                                                                                                                          gCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGAc       >  2:501710/1‑149 (MQ=255)
                                                                                                                                             gATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCa    <  2:4013340/149‑1 (MQ=255)
                                                                                                                                             gATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCa    <  2:675764/149‑1 (MQ=255)
                                                                                                                                               tttAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCAcc  >  1:3438735/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCACC  >  CP009273/2217876‑2218167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑