Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,223,247 C→T 100% W206* (TGG→TGA)  dusC ← tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,223,2470CT98.0% 817.4 / 4.7 248W206* (TGG→TGA) dusCtRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (117/126);  total (120/128)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 248 total aligned reads displayed.

TTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTT  >  CP009273/2223102‑2223392
                                                                                                                                                 |                                                                                                                                                 
tttGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAg                                                                                                                                                >  2:912662/1‑149 (MQ=255)
tttGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAg                                                                                                                                                <  1:746633/149‑1 (MQ=255)
tttGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAg                                                                                                                                                >  2:2671982/1‑149 (MQ=255)
 ttGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGt                                                                                                                                               <  2:449327/149‑1 (MQ=255)
         aagcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGAtt                                                                                                                                       >  2:3134524/1‑149 (MQ=255)
          agcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGAttt                                                                                                                                      <  1:3988203/149‑1 (MQ=255)
           gcaaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGAGTTc                                                                                                                                     >  1:444504/1‑149 (MQ=255)
             aaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCAc                                                                                                                                   >  1:3713568/1‑149 (MQ=255)
             aaCCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCAGTGTTGCGCGCTCTGTCAGTCCCAGATTTCAc                                                                                                                                   >  2:1315557/1‑149 (MQ=255)
               ccaccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCg                                                                                                                                 >  2:2364614/1‑149 (MQ=255)
                caccTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGt                                                                                                                                >  2:2844601/1‑149 (MQ=255)
                 accTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGtt                                                                                                                               <  2:4014916/149‑1 (MQ=255)
                 accTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGtt                                                                                                                               <  1:2357811/149‑1 (MQ=255)
                 accTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGtt                                                                                                                               <  1:2357790/149‑1 (MQ=255)
                 accTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATGTCACCGtt                                                                                                                               >  2:3900309/1‑149 (MQ=255)
                   cTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTgg                                                                                                                             >  2:2912531/1‑149 (MQ=255)
                   cTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTgg                                                                                                                             <  2:892972/149‑1 (MQ=255)
                    tCCGGCCACGGCATTCGCGGTTCGTTATAGTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATGTCACCGTTGGc                                                                                                                            >  2:498026/1‑149 (MQ=255)
                      ctgCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCaa                                                                                                                          >  1:2652311/3‑149 (MQ=255)
                        gCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATc                                                                                                                        <  1:840091/149‑1 (MQ=255)
                         ccACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCa                                                                                                                       <  1:16123/149‑1 (MQ=255)
                         ccACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCa                                                                                                                       <  1:1187482/149‑1 (MQ=255)
                         ccACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCa                                                                                                                       <  1:3430910/149‑1 (MQ=255)
                           aCGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCAcc                                                                                                                     >  1:1276011/1‑149 (MQ=255)
                             ggCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCgg                                                                                                                   >  1:3176684/1‑149 (MQ=255)
                              gCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGa                                                                                                                  >  1:1103212/1‑149 (MQ=255)
                              gCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGa                                                                                                                  <  1:3612335/149‑1 (MQ=255)
                               cATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGaa                                                                                                                 <  1:2750554/149‑1 (MQ=255)
                                  tCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAatat                                                                                                              <  2:2241560/149‑1 (MQ=255)
                                  tCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAatat                                                                                                              <  1:4076052/149‑1 (MQ=255)
                                   cgcgGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATAtt                                                                                                             <  2:3528023/149‑1 (MQ=255)
                                      ggTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCCCCGTTGGCAATCACCGGACTCTTCAg                                                                                                          >  2:3780352/1‑149 (MQ=255)
                                       gTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGc                                                                                                         >  2:2991657/1‑149 (MQ=255)
                                       gTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGc                                                                                                         >  1:2370395/1‑149 (MQ=255)
                                        ttCGTTATATTTTACCACCCTGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGcc                                                                                                        <  1:1500217/149‑1 (MQ=255)
                                        ttCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCCTCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGcc                                                                                                        <  1:2775876/149‑1 (MQ=255)
                                            ttATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTg                                                                                                    <  1:1061976/149‑1 (MQ=255)
                                            ttATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTg                                                                                                    <  2:2079749/149‑1 (MQ=255)
                                            ttATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTg                                                                                                    >  1:3387406/1‑149 (MQ=255)
                                               tatTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCg                                                                                                 >  1:199531/1‑149 (MQ=255)
                                               tatTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCAAGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCg                                                                                                 >  1:2449306/1‑149 (MQ=255)
                                                 ttttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCCGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGaa                                                                                               >  2:2860499/1‑149 (MQ=255)
                                                 ttttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGaa                                                                                               <  2:1657935/149‑1 (MQ=255)
                                                   ttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAata                                                                                             <  2:2409503/149‑1 (MQ=255)
                                                   ttACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAata                                                                                             <  1:2904841/149‑1 (MQ=255)
                                                       caccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGc                                                                                         >  2:3456332/1‑149 (MQ=255)
                                                           cGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAAt                                                                                     <  1:388254/149‑1 (MQ=255)
                                                           cGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAAt                                                                                     <  1:1355360/149‑1 (MQ=255)
                                                            ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATc                                                                                    <  1:1731890/149‑1 (MQ=255)
                                                            ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATc                                                                                    >  1:3243451/1‑149 (MQ=255)
                                                            ggCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACGGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATc                                                                                    <  1:2145991/149‑1 (MQ=255)
                                                                   gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGcc                                                                             >  2:639688/1‑149 (MQ=255)
                                                                   gTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGcc                                                                             >  1:2678898/1‑149 (MQ=255)
                                                                       ggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTc                                                                         <  2:140884/149‑1 (MQ=255)
                                                                         aaTATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCaa                                                                       <  1:4010741/149‑1 (MQ=255)
                                                                         aaTATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCaa                                                                       >  2:2643360/1‑149 (MQ=255)
                                                                         aaTATTGAGAGCCCCGCGACCAATCATCACAGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCATTCCCACAAAACACCTATGGCAATCACCGGAAAAATCAGCCGCAGGCGAAAATCTCCAAACGCCTGCCAGTCaa                                                                       >  1:693150/1‑149 (MQ=255)
                                                                            atTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAatat                                                                    >  2:1827379/1‑149 (MQ=255)
                                                                              tGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGc                                                                  <  2:802793/149‑1 (MQ=255)
                                                                               gAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCt                                                                 >  1:1723398/1‑149 (MQ=255)
                                                                                 gcgcCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTcc                                                               <  2:1276011/149‑1 (MQ=255)
                                                                                 gcgcCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTcc                                                               <  1:993363/149‑1 (MQ=255)
                                                                                 gcgcCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTcc                                                               <  2:3741178/149‑1 (MQ=255)
                                                                                        gcgACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGt                                                        <  1:912628/149‑1 (MQ=255)
                                                                                        gcgACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGt                                                        <  1:1132310/149‑1 (MQ=255)
                                                                                           aCCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAc                                                     >  2:3183940/1‑149 (MQ=255)
                                                                                           aCCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATAGACAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAc                                                     <  1:289908/149‑1 (MQ=255)
                                                                                            ccAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAcc                                                    <  1:1671258/149‑1 (MQ=255)
                                                                                             cAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAAccc                                                   <  1:1239434/149‑1 (MQ=255)
                                                                                              aaTCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCt                                                  >  2:3500640/1‑149 (MQ=255)
                                                                                               atcatcACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTg                                                 >  2:141422/1‑149 (MQ=255)
                                                                                                tcatcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGc                                                >  2:3560168/1‑149 (MQ=255)
                                                                                                  atcaCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGctc                                              <  1:3134524/149‑1 (MQ=255)
                                                                                                     aCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCttt                                           <  1:571746/149‑1 (MQ=255)
                                                                                                          gTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTcc                                      <  2:1746286/149‑1 (MQ=255)
                                                                                                           tCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCg                                     >  2:2044612/1‑149 (MQ=255)
                                                                                                                   gCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGca                             >  1:486865/1‑149 (MQ=255)
                                                                                                                   gCTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTCGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGca                             >  2:713409/1‑149 (MQ=255)
                                                                                                                    cTGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcac                            >  1:3191350/1‑149 (MQ=255)
                                                                                                                     tGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcacc                           >  1:145777/1‑149 (MQ=255)
                                                                                                                     tGATCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcacc                           >  1:824254/1‑149 (MQ=255)
                                                                                                                       aTCGCCATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGcaccac                         >  1:2053828/1‑149 (MQ=255)
                                                                                                                          gCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAg                      >  2:2732914/1‑149 (MQ=255)
                                                                                                                           ccATGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGc                     >  2:2255799/1‑149 (MQ=255)
                                                                                                                             aTGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTc                   >  1:2815084/1‑149 (MQ=255)
                                                                                                                             aTGCATTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTc                   >  2:1173596/1‑149 (MQ=255)
                                                                                                                                 aTTGTTGCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa               <  1:3745036/149‑1 (MQ=255)
                                                                                                                                  ttgttgCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAg              >  2:386414/1‑149 (MQ=255)
                                                                                                                                      tgCGCGCTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGcc          <  2:2371496/149‑1 (MQ=255)
                                                                                                                                       gcgcgcTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCa         <  1:141422/149‑1 (MQ=255)
                                                                                                                                       gcgcgcTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCa         <  1:140972/149‑1 (MQ=255)
                                                                                                                                          cgcTCTGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGcc      <  1:3561818/149‑1 (MQ=255)
                                                                                                                                           gcTCTGTCAGTCCCAGATTTCACCGTTGGCAATAACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCt     >  1:1823038/1‑149 (MQ=255)
                                                                                                                                            ctctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTg    <  1:634899/149‑1 (MQ=255)
                                                                                                                                             tctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGt   <  2:3962995/149‑1 (MQ=255)
                                                                                                                                             tctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGt   <  2:2155165/149‑1 (MQ=255)
                                                                                                                                              ctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGtt  <  2:1586606/149‑1 (MQ=255)
                                                                                                                                              ctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGtt  <  2:243784/149‑1 (MQ=255)
                                                                                                                                              ctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGtt  <  2:767423/149‑1 (MQ=255)
                                                                                                                                              ctGTCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGtt  <  2:243666/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTTGCAGCAAAGCAACCACCTCCGGCCACGGCATTCGCGGTTCGTTATATTTTACCACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCGGAATATTCAGCCGCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTT  >  CP009273/2223102‑2223392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑