Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,234,532 C→A 100% D206Y (GAT→TAT)  galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,234,5320CA99.6% 865.9 / ‑4.4 266D206Y (GAT→TAT) galSgalactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (125/139);  total (126/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 269 total aligned reads displayed.

GTCAGTGCGCCAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATCCTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCGGGCACCGCTGAGATTATCCAGG  >  CP009273/2234387‑2234677
                                                                                                                                                 |                                                                                                                                                 
   aGTGCGCCAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTGAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGctc                                                                                                                                             >  1:2995216/1‑149 (MQ=255)
   aGTGCGCCAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGctc                                                                                                                                             <  1:1608007/149‑1 (MQ=255)
   aGTGCGCCAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGctc                                                                                                                                             >  2:149841/1‑149 (MQ=255)
    gTGCGCCAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGctct                                                                                                                                            >  2:2301157/1‑149 (MQ=255)
    gTGCGCCAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGctct                                                                                                                                            >  1:581440/1‑149 (MQ=255)
     tGCGCCAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCtt                                                                                                                                           <  1:635704/149‑1 (MQ=255)
         ccAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCaa                                                                                                                                       <  2:2810243/149‑1 (MQ=255)
         ccAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCaa                                                                                                                                       <  2:2041534/149‑1 (MQ=255)
           aGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACg                                                                                                                                     >  2:1277486/1‑149 (MQ=255)
            gCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGc                                                                                                                                    >  2:3788149/1‑149 (MQ=255)
               gCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACt                                                                                                                                 >  2:254493/1‑149 (MQ=255)
               gCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACt                                                                                                                                 >  2:4100006/1‑149 (MQ=255)
                ccATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTc                                                                                                                                >  2:2402494/1‑149 (MQ=255)
                ccATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTc                                                                                                                                >  1:3683082/1‑149 (MQ=255)
                 cATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCa                                                                                                                               >  2:3174654/1‑149 (MQ=255)
                  atatTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGACTAATATACTGCTCTTTCAACGCACTCAt                                                                                                                              >  2:2960981/1‑149 (MQ=255)
                  atatTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCAt                                                                                                                              >  1:3004594/1‑149 (MQ=255)
                     ttGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCa                                                                                                                           <  2:3656770/149‑1 (MQ=255)
                       gTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGc                                                                                                                         >  1:3763915/1‑149 (MQ=255)
                        tCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGcc                                                                                                                        >  1:3410272/1‑149 (MQ=255)
                           ttATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGc                                                                                                                     <  1:3239441/149‑1 (MQ=255)
                           ttATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGc                                                                                                                     <  1:3293873/149‑1 (MQ=255)
                             ataAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCtt                                                                                                                   <  1:2141191/149‑1 (MQ=255)
                              taAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCttt                                                                                                                  <  1:783084/149‑1 (MQ=255)
                              taAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGATCTTTCAACGCACTCATCCAGCCTGCttt                                                                                                                  >  2:3458595/1‑149 (MQ=255)
                                 gCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACg                                                                                                               >  1:3959425/1‑149 (MQ=255)
                                 gCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACg                                                                                                               >  1:2494156/1‑149 (MQ=255)
                                      tACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATgg                                                                                                          >  1:3728820/1‑149 (MQ=255)
                                       aCAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGc                                                                                                         >  1:2825124/1‑149 (MQ=255)
                                       aCAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGc                                                                                                         <  2:223568/149‑1 (MQ=255)
                                         aGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGt                                                                                                       >  2:2275342/1‑149 (MQ=255)
                                              tAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCt                                                                                                  >  2:2715249/1‑149 (MQ=255)
                                              tAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCt                                                                                                  <  1:149841/149‑1 (MQ=255)
                                              tAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCt                                                                                                  >  2:2511648/1‑149 (MQ=255)
                                              tAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCt                                                                                                  <  2:3243723/149‑1 (MQ=255)
                                               aaGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCtt                                                                                                 <  1:1292815/149‑1 (MQ=255)
                                                aGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTc                                                                                                >  2:645420/1‑149 (MQ=255)
                                                  ttGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATTTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCaa                                                                                              >  1:448276/1‑149 (MQ=255)
                                                  ttGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCaa                                                                                              >  2:1331206/1‑149 (MQ=255)
                                                   tGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAAt                                                                                             <  1:3276498/149‑1 (MQ=255)
                                                        aTTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGt                                                                                        >  2:2614836/1‑149 (MQ=255)
                                                        aTTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGt                                                                                        >  1:2093162/1‑149 (MQ=255)
                                                         ttGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTg                                                                                       <  1:3643671/149‑1 (MQ=255)
                                                           gcgcCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGc                                                                                     <  1:3923137/149‑1 (MQ=255)
                                                             gcCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTg                                                                                   <  2:584348/149‑1 (MQ=255)
                                                              ccccAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTgg                                                                                  >  1:2547094/1‑149 (MQ=255)
                                                              ccccAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTgg                                                                                  <  1:2348212/149‑1 (MQ=255)
                                                              ccccAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTgg                                                                                  >  1:1215039/1‑149 (MQ=255)
                                                               cccAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGga                                                                                 <  1:2645523/149‑1 (MQ=255)
                                                               cccAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGga                                                                                 <  1:2201186/149‑1 (MQ=255)
                                                                ccAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGgaa                                                                                <  1:2620868/149‑1 (MQ=255)
                                                                ccAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGgaa                                                                                <  2:820202/149‑1 (MQ=255)
                                                                 cagcagTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGgaag                                                                               >  2:3704295/1‑149 (MQ=255)
                                                                      gTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGaagaaaga                                                                          >  2:3927988/1‑149 (MQ=255)
                                                                       gtCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGAt                                                                         <  1:1111263/148‑1 (MQ=255)
                                                                         cAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGTAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATaa                                                                       <  1:1425768/149‑1 (MQ=255)
                                                                         cAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATaa                                                                       <  1:26871/149‑1 (MQ=255)
                                                                          aaCCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAAc                                                                      >  1:1461935/1‑149 (MQ=255)
                                                                            ccATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCa                                                                    <  1:1885644/149‑1 (MQ=255)
                                                                             cATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCaa                                                                   >  2:1723011/1‑149 (MQ=255)
                                                                             cATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCaa                                                                   <  1:3323863/149‑1 (MQ=255)
                                                                             cATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCaa                                                                   <  2:861729/149‑1 (MQ=255)
                                                                               tCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATa                                                                 <  2:3374403/149‑1 (MQ=255)
                                                                                     ccTCACCGCCCGGCATGTCCGGCGTACCAGCGCCTATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTtga                                                           <  2:3061283/149‑1 (MQ=255)
                                                                                     ccTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTtga                                                           <  1:3386727/149‑1 (MQ=255)
                                                                                     ccTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTtga                                                           <  1:271555/149‑1 (MQ=255)
                                                                                      cTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTCCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTtgat                                                          >  1:3637916/1‑149 (MQ=255)
                                                                                       tCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTtgatg                                                         <  1:3491094/149‑1 (MQ=255)
                                                                                       tCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTtgatg                                                         >  2:1855540/1‑149 (MQ=255)
                                                                                       tCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTtgatg                                                         >  1:1678726/1‑149 (MQ=255)
                                                                                          ccGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGAcc                                                      >  1:695224/1‑149 (MQ=255)
                                                                                            gCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGt                                                    <  2:3342888/149‑1 (MQ=255)
                                                                                             cccGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGtt                                                   <  1:3106004/149‑1 (MQ=255)
                                                                                              ccGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCTGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTa                                                  <  2:3717198/149‑1 (MQ=255)
                                                                                              ccGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTa                                                  >  1:3052904/1‑149 (MQ=255)
                                                                                               cGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCCTCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTAt                                                 <  2:2749813/149‑1 (MQ=255)
                                                                                               cGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATCGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTAt                                                 <  1:3847788/149‑1 (MQ=255)
                                                                                                ggCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATACTATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGGGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTAtt                                                <  2:2178449/149‑1 (MQ=255)
                                                                                                ggCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTAtt                                                >  2:2628914/1‑149 (MQ=255)
                                                                                                 gCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTc                                               <  2:1830945/149‑1 (MQ=255)
                                                                                                 gCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTc                                               <  1:918561/149‑1 (MQ=255)
                                                                                                     gTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCa                                           >  2:3849226/1‑149 (MQ=255)
                                                                                                      tCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCaa                                          <  1:1153756/149‑1 (MQ=255)
                                                                                                       ccGGCGTACCCGCGCCAATCCAGCGGTCCGGCGGAATACTATCCTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAAc                                         <  2:2786394/149‑1 (MQ=255)
                                                                                                       ccGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAAc                                         <  2:724244/149‑1 (MQ=255)
                                                                                                            gTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATgcg                                    >  1:1443112/1‑149 (MQ=255)
                                                                                                             tACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATgcgc                                   >  1:3164658/1‑149 (MQ=255)
                                                                                                             tACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATgcgc                                   >  1:3162085/1‑149 (MQ=255)
                                                                                                             tACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATgcgc                                   >  1:1245226/1‑149 (MQ=255)
                                                                                                               ccAGCGCCAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGt                                 <  2:1009477/149‑1 (MQ=255)
                                                                                                                     ccAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCAt                           >  2:2834864/1‑149 (MQ=255)
                                                                                                                     ccAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCAt                           <  1:1922574/149‑1 (MQ=255)
                                                                                                                     ccAATCCAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCAt                           <  2:761369/149‑1 (MQ=255)
                                                                                                                          ccAGCTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCggg                      <  2:1627959/149‑1 (MQ=255)
                                                                                                                              cTTTCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCGGGCAcc                  >  2:3399509/1‑149 (MQ=255)
                                                                                                                                ttCCGGCGGAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCGGGCACCGc                <  1:1769113/149‑1 (MQ=255)
                                                                                                                                       ggAATAATATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCGGGCACCGCTGAGAtt         >  2:2335215/1‑149 (MQ=255)
                                                                                                                                         aataatATACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCGGGCACCGCTGAGATTAt       <  2:3763915/149‑1 (MQ=255)
                                                                                                                                          ataataTACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCGGGCACCGCTGAGATTATc      >  2:922239/1‑149 (MQ=255)
                                                                                                                                           taataTACTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCGGGCACCGCTGAGATTATcc     <  2:3315123/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GTCAGTGCGCCAGCGGCCATATTGTCGTTATAAGCAAATACAGCGGTAAGTTGTAGATTGCGCCCCAGCAGTTCAACCATCGCCGCCTCACCGCCCGGCATGTCCGGCGTACCAGCGCCAATCCAGCTTTCCGGCGGAATAATATCCTGCTCTTTCAACGCACTCATCCAGCCTGCTTTACGCATGGCGTCATCTTCAATGCCGTGGCTGGAAGAAAGATAACCAATACGTTGATGACCGTTATTCAGCAACATGCGCGTCGCCATTCGGGCACCGCTGAGATTATCCAGG  >  CP009273/2234387‑2234677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑