Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,248,657 C→A 100% K3N (AAG→AAT)  rihB ← ribonucleoside hydrolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,248,6570CA98.2% 704.8 / 3.1 224K3N (AAG→AAT) rihBribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (118/102);  total (121/103)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

CATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAA  >  CP009273/2248509‑2248800
                                                                                                                                                    |                                                                                                                                               
     aGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTc                                                                                                                                            >  2:2327966/1‑149 (MQ=255)
      ggCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTcc                                                                                                                                           <  2:2921266/149‑1 (MQ=255)
      ggCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTcc                                                                                                                                           >  2:3477986/1‑149 (MQ=255)
        ccATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCAt                                                                                                                                         <  2:1182321/149‑1 (MQ=255)
            taattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGtt                                                                                                                                     <  2:755053/149‑1 (MQ=255)
            taattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGtt                                                                                                                                     <  1:3696072/149‑1 (MQ=255)
              attaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                   >  2:3549892/1‑149 (MQ=255)
              attaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                   <  1:3627183/149‑1 (MQ=255)
              attaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                   >  2:3550487/1‑149 (MQ=255)
               ttaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAt                                                                                                                                  >  1:2122775/1‑149 (MQ=255)
                taatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAtt                                                                                                                                 >  1:3161135/1‑149 (MQ=255)
                 aatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAttt                                                                                                                                <  1:418132/149‑1 (MQ=255)
                 aatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAttt                                                                                                                                <  1:418693/149‑1 (MQ=255)
                  atGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTc                                                                                                                               <  2:667216/149‑1 (MQ=255)
                     ttttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGTATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                            >  2:3738289/1‑149 (MQ=255)
                     ttttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                            >  2:557972/1‑149 (MQ=255)
                     ttttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                            <  2:614007/149‑1 (MQ=255)
                      tttATCCAGCGTCTGATTACCCGCTACAATAGTGATGCCGAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctct                                                                                                                           <  1:3194402/149‑1 (MQ=255)
                        tATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGt                                                                                                                         >  2:1671703/1‑149 (MQ=255)
                        tATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGt                                                                                                                         <  2:32363/149‑1 (MQ=255)
                        tATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGt                                                                                                                         <  2:2111261/149‑1 (MQ=255)
                          tCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAAAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGttn                                                                                                                       <  1:1344258/149‑2 (MQ=255)
                           cAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTc                                                                                                                      <  2:3156520/149‑1 (MQ=255)
                              gCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAg                                                                                                                   <  2:3992039/149‑1 (MQ=255)
                                gTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGtt                                                                                                                 >  2:2668504/1‑149 (MQ=255)
                                      ttACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTa                                                                                                           >  2:933266/1‑149 (MQ=255)
                                       tACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAt                                                                                                          >  2:1762208/1‑149 (MQ=255)
                                        aCCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAtt                                                                                                         <  1:1883148/149‑1 (MQ=255)
                                          ccGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAtttt                                                                                                       <  2:3041373/149‑1 (MQ=255)
                                          ccGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAtttt                                                                                                       >  2:378314/1‑149 (MQ=255)
                                             cTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTc                                                                                                    >  2:2124455/1‑149 (MQ=255)
                                             cTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTc                                                                                                    <  2:3746698/149‑1 (MQ=255)
                                             cTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTc                                                                                                    >  2:49329/1‑149 (MQ=255)
                                              tACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCt                                                                                                   >  2:585348/1‑149 (MQ=255)
                                                 aaTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACa                                                                                                <  2:1565355/149‑1 (MQ=255)
                                                 aaTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACa                                                                                                <  2:1403866/149‑1 (MQ=255)
                                                 aaTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACa                                                                                                <  2:1063661/149‑1 (MQ=255)
                                                  aTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAg                                                                                               >  1:2681979/1‑149 (MQ=255)
                                                     gTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCaa                                                                                            >  2:1382245/1‑149 (MQ=255)
                                                        aTGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGa                                                                                         >  2:1460005/1‑149 (MQ=255)
                                                           ccTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGaaaa                                                                                      >  1:2011201/1‑149 (MQ=255)
                                                            cTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAAt                                                                                     <  1:1262781/149‑1 (MQ=255)
                                                             taataaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAAtt                                                                                    >  2:448975/1‑149 (MQ=255)
                                                             taataaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAAtt                                                                                    <  1:1070253/149‑1 (MQ=255)
                                                              aataaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTc                                                                                   >  1:1168984/1‑149 (MQ=255)
                                                                taaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGa                                                                                 >  1:1603250/1‑149 (MQ=255)
                                                                  aaTCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGAcc                                                                               <  2:3047740/149‑1 (MQ=255)
                                                                   aTCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCg                                                                              >  2:229494/1‑149 (MQ=255)
                                                                        ttGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCg                                                                         >  1:3706680/1‑149 (MQ=255)
                                                                          gCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgat                                                                       <  2:1225092/149‑1 (MQ=255)
                                                                             ggATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatgat                                                                    <  2:4025225/149‑1 (MQ=255)
                                                                             ggATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatgat                                                                    <  1:542014/149‑1 (MQ=255)
                                                                             ggATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGAACACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatgat                                                                    <  1:2990281/149‑1 (MQ=255)
                                                                              gATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGAtt                                                                   >  2:401296/1‑149 (MQ=255)
                                                                                 gTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGaa                                                                >  2:3315224/1‑149 (MQ=255)
                                                                                 gTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGaa                                                                >  2:1809997/1‑149 (MQ=255)
                                                                                   ttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATc                                                              <  1:262995/149‑1 (MQ=255)
                                                                                       ccgccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaa                                                          <  1:2180278/149‑1 (MQ=255)
                                                                                       ccgccATAATTATAGCAATAGCATCATCATGACCCGGAACAAAATCCAGAATAATTTTTCTATTTTCAATTTTTTATTTCCTCTGTTTCCAGTTGCTTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaa                                                          <  1:1762208/149‑1 (MQ=255)
                                                                                         gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaaca                                                        >  1:1226467/1‑149 (MQ=255)
                                                                                          ccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaacaa                                                       <  2:1647105/149‑1 (MQ=255)
                                                                                            atcatTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAacaaca                                                     >  1:379554/1‑149 (MQ=255)
                                                                                             tcatTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAg                                                    >  1:4096773/1‑149 (MQ=255)
                                                                                               atTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTGTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcg                                                  >  1:3448712/1‑149 (MQ=255)
                                                                                                 tataGCAATAGAATCATCATGACCCGTATCACAATCCAGAATAATTTTTCTATTTTCATTTTTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgta                                                <  1:2668504/149‑1 (MQ=255)
                                                                                                 tataGAAATAGAAACATCATGACCCGTAACACAATCAATAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCTGTTGGTTTATTTTTTCTACACAAAATAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgta                                                <  1:348076/149‑1 (MQ=255)
                                                                                                   tagcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacg                                              <  1:250185/149‑1 (MQ=255)
                                                                                                    agcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgt                                             >  2:15820/1‑149 (MQ=255)
                                                                                                     gcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgta                                            <  2:1937601/149‑1 (MQ=255)
                                                                                                       aatagcaTCATCATGACCCGGATCACAATCCATAATAATTTTTCTATTTTCCTTTGTTTATTTCCTCTGTTTCGAGTTGCGTTTTTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATg                                          <  1:893976/149‑1 (MQ=255)
                                                                                                         tagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTTTTTATTTCCTCTTTTTCCAGTTGTGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCt                                        <  2:1296965/149‑1 (MQ=255)
                                                                                                          agcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTa                                       <  2:3492237/149‑1 (MQ=255)
                                                                                                           gcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaa                                      >  2:3058016/1‑149 (MQ=255)
                                                                                                              tcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAata                                   <  2:2746195/149‑1 (MQ=255)
                                                                                                                 tcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATga                                >  2:1018337/1‑149 (MQ=255)
                                                                                                                  catGACCCGTATCACAATCCAGAATAATTTTTCTATTTTCCTTTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                               <  1:1515949/149‑1 (MQ=255)
                                                                                                                  catGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                               >  1:1189637/1‑149 (MQ=255)
                                                                                                                   atGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgaga                              >  2:2431554/1‑149 (MQ=255)
                                                                                                                     gACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGaaa                            >  2:780753/1‑149 (MQ=255)
                                                                                                                     gACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGaaa                            >  2:780544/1‑149 (MQ=255)
                                                                                                                     gACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGaaa                            <  2:674200/149‑1 (MQ=255)
                                                                                                                      aCCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAt                           >  1:1497322/1‑149 (MQ=255)
                                                                                                                      aCCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTAAAGTTGCGTTATTTTTTATAAAGCAAAGAAAATTCTACCGGGCCGATGATTGAAACTTAACAAAATCGTACGTATGCTAAAAATGAGaaaa                           >  1:840570/1‑148 (MQ=255)
                                                                                                                         cGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtctc                        >  1:257032/1‑149 (MQ=255)
                                                                                                                         cGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtctc                        >  2:3873822/1‑149 (MQ=255)
                                                                                                                          ggATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCa                       >  2:1807690/1‑149 (MQ=255)
                                                                                                                          ggATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCa                       <  1:1188153/149‑1 (MQ=255)
                                                                                                                           gATCACAATCCATAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCAt                      <  1:1472914/149‑1 (MQ=255)
                                                                                                                                 aaTCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGa                <  1:200989/149‑1 (MQ=255)
                                                                                                                                   tCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATa              >  1:2749772/1‑149 (MQ=255)
                                                                                                                                    ccAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATaa             <  1:2625156/149‑1 (MQ=255)
                                                                                                                                     cAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATaaa            <  1:2327966/149‑1 (MQ=255)
                                                                                                                                      aGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAAc           >  1:1169864/1‑149 (MQ=255)
                                                                                                                                       gAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACa          <  2:379554/149‑1 (MQ=255)
                                                                                                                                       gAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACa          <  1:3983335/149‑1 (MQ=255)
                                                                                                                                         ataatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATc        <  1:49329/149‑1 (MQ=255)
                                                                                                                                         ataatTTTTCTATTTTCCATTGTTTATTTCATCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCTACCGGGCCCATGATTGAATATTAACAACAGCGTACTTATGCTAAAAATGAGAAATATAAAATCGGATaaaaaac        >  1:3273112/1‑145 (MQ=255)
                                                                                                                                             tttttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTga    <  2:2188664/149‑1 (MQ=255)
                                                                                                                                              ttttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaa   <  1:1671703/149‑1 (MQ=255)
                                                                                                                                               tttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaa  >  2:1981217/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAA  >  CP009273/2248509‑2248800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑