BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,277,743	A→T	38.9%	N46Y (AAC→TAC)	yejL →	UPF0352 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,277,743	0	A	T	38.9%	259.4 / inf	293	N46Y (AAC→TAC)	yejL	UPF0352 family protein
Reads supporting (aligned to +/- strand): ref base A (79/100); new base T (61/53); total (140/153)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

GATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCT  >  CP009273/2277597‑2277891

gATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAac                                                                                                                                                    <  1:2650136/149‑1 (MQ=255)

 aTTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAaca                                                                                                                                                   >  2:973089/1‑149 (MQ=255)

 aTTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAaca                                                                                                                                                   >  1:2046253/1‑149 (MQ=255)

   ttCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACAcc                                                                                                                                                 >  2:1044773/1‑149 (MQ=255)

       aTTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCa                                                                                                                                             >  2:1106852/1‑149 (MQ=255)

        tttATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCAt                                                                                                                                            >  1:1465724/1‑149 (MQ=255)

        tttATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCAt                                                                                                                                            >  1:2878137/1‑149 (MQ=255)

        tttATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCAt                                                                                                                                            <  1:2371039/149‑1 (MQ=255)

         ttATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCAtt                                                                                                                                           <  1:650307/149‑1 (MQ=255)

         ttATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCAtt                                                                                                                                           <  2:1262139/149‑1 (MQ=255)

          tATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTg                                                                                                                                          >  2:2769564/1‑149 (MQ=255)

             gCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGccc                                                                                                                                       >  2:798000/1‑149 (MQ=255)

               cacaAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCg                                                                                                                                     >  2:2549298/1‑149 (MQ=255)

               cacaAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCg                                                                                                                                     >  2:954022/1‑149 (MQ=255)

                     tttCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCaa                                                                                                                               >  1:55813/1‑149 (MQ=255)

                         ccGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGcc                                                                                                                           >  1:3667781/1‑149 (MQ=255)

                         ccGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGcc                                                                                                                           <  1:2447216/149‑1 (MQ=255)

                               cAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCaa                                                                                                                     <  1:1173593/149‑1 (MQ=255)

                                   gatgaACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGc                                                                                                                 >  2:3051655/1‑149 (MQ=255)

                                       aaCAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAAc                                                                                                             <  1:126435/149‑1 (MQ=255)

                                        aCAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCACTTGCCAAct                                                                                                            >  2:2601762/1‑149 (MQ=255)

                                         cAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAActc                                                                                                           <  1:189309/149‑1 (MQ=255)

                                          aGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAActct                                                                                                          <  1:2212805/149‑1 (MQ=255)

                                          aGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAActct                                                                                                          >  2:3610557/1‑149 (MQ=255)

                                            gTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCttt                                                                                                        <  1:840736/149‑1 (MQ=255)

                                               gAACAATTGCTCGCCGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGc                                                                                                     >  1:3589610/1‑149 (MQ=255)

                                                 aCAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCACCGCCAGGCAATTGCCAACTCTTTTGccc                                                                                                   >  1:74253/1‑149 (MQ=255)

                                                   aaTTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCcgc                                                                                                 >  1:755637/1‑149 (MQ=255)

                                                   aaTTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCcgc                                                                                                 <  1:1312858/149‑1 (MQ=255)

                                                    aTTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCCGGCAATTGCCAACTCTTTTGCCcgcg                                                                                                >  1:2390771/1‑149 (MQ=255)

                                                    aTTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCcgcg                                                                                                >  1:610962/1‑149 (MQ=255)

                                                         tCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTa                                                                                           <  2:498990/149‑1 (MQ=255)

                                                             agagCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGt                                                                                       >  2:1814356/1‑149 (MQ=255)

                                                             agagCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGt                                                                                       >  2:598665/1‑149 (MQ=255)

                                                               agCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTcc                                                                                     <  1:3847868/149‑1 (MQ=255)

                                                                 ctgctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCctc                                                                                   <  2:1446896/149‑1 (MQ=255)

                                                                   gctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTa                                                                                 <  2:379334/149‑1 (MQ=255)

                                                                    ctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTAt                                                                                <  1:93833/149‑1 (MQ=255)

                                                                     tCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATc                                                                               <  2:1941095/149‑1 (MQ=255)

                                                                     tCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATc                                                                               >  1:304771/1‑149 (MQ=255)

                                                                       aaCGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCaa                                                                             <  2:3268319/149‑1 (MQ=255)

                                                                       aaCGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCaa                                                                             >  2:1664955/1‑149 (MQ=255)

                                                                       aaCGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCaa                                                                             >  2:2371989/1‑149 (MQ=255)

                                                                        aCGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAAc                                                                            >  1:281530/1‑149 (MQ=255)

                                                                           tACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGaa                                                                         >  1:3972995/1‑149 (MQ=255)

                                                                            aCTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAg                                                                        >  1:3288870/1‑149 (MQ=255)

                                                                             cTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGa                                                                       <  2:545503/149‑1 (MQ=255)

                                                                               ggAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACa                                                                     >  1:3144731/1‑149 (MQ=255)

                                                                               ggAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACa                                                                     >  1:3105481/1‑149 (MQ=255)

                                                                               ggAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACa                                                                     >  1:1847569/1‑149 (MQ=255)

                                                                               ggAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACa                                                                     <  2:3833007/149‑1 (MQ=255)

                                                                                gAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACaa                                                                    >  1:2849797/1‑149 (MQ=255)

                                                                                 aaaaaCATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACaaa                                                                   <  2:2046253/149‑1 (MQ=255)

                                                                                  aaaaCATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAg                                                                  >  2:3962912/1‑149 (MQ=255)

                                                                                      cATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCa                                                              <  1:1005071/149‑1 (MQ=255)

                                                                                      cATAAGGCTCCGACCGACCTGTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCa                                                              <  2:2481135/149‑1 (MQ=255)

                                                                                       aTAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAACGCGCAc                                                             >  2:2830999/1‑149 (MQ=255)

                                                                                       aTAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCAc                                                             <  2:4068696/149‑1 (MQ=255)

                                                                                             cTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAggg                                                       <  2:369153/149‑1 (MQ=255)

                                                                                              tCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGa                                                      <  2:3633246/149‑1 (MQ=255)

                                                                                                  accgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACa                                                  <  1:195413/149‑1 (MQ=255)

                                                                                                  accgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACa                                                  <  1:996516/149‑1 (MQ=255)

                                                                                                  accgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACa                                                  >  1:1026560/1‑149 (MQ=255)

                                                                                                  accgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACa                                                  <  1:997224/149‑1 (MQ=255)

                                                                                                   ccgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAg                                                 >  2:331699/1‑149 (MQ=255)

                                                                                                   ccgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAg                                                 >  2:35783/1‑149 (MQ=255)

                                                                                                    cgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGa                                                <  2:1837901/149‑1 (MQ=255)

                                                                                                    cgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGa                                                <  1:3216165/149‑1 (MQ=255)

                                                                                                    cgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGa                                                >  1:1108442/1‑149 (MQ=255)

                                                                                                      accTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATa                                              >  1:3038075/1‑149 (MQ=255)

                                                                                                       ccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATaa                                             <  1:1545959/149‑1 (MQ=255)

                                                                                                       ccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATaa                                             >  1:2524963/1‑149 (MQ=255)

                                                                                                          ttCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAg                                          <  2:2401831/149‑1 (MQ=255)

                                                                                                          ttCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAg                                          <  2:3359136/149‑1 (MQ=255)

                                                                                                          gtCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAg                                          <  2:1797038/148‑1 (MQ=255)

                                                                                                            cccTGATGGTGTTAGGCAACATGGTCACTAACCATATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGt                                        <  1:2458478/149‑1 (MQ=255)

                                                                                                             ccTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGtt                                       >  2:451391/1‑149 (MQ=255)

                                                                                                               tGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCACTTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTAt                                     >  2:4021831/1‑149 (MQ=255)

                                                                                                               tGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTAt                                     <  1:3360637/149‑1 (MQ=255)

                                                                                                                   ggTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTa                                 <  1:1395576/149‑1 (MQ=255)

                                                                                                                    gtgtTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTaa                                <  1:1999002/149‑1 (MQ=255)

                                                                                                                    gtgtTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTaa                                <  1:3282388/149‑1 (MQ=255)

                                                                                                                         aGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCAt                           <  2:400622/149‑1 (MQ=255)

                                                                                                                         aGGCAACATGGTCACTAACCTGATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCAt                           <  2:2630548/149‑1 (MQ=255)

                                                                                                                          ggCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATc                          <  2:2173452/149‑1 (MQ=255)

                                                                                                                           gCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATcn                         <  1:486666/149‑2 (MQ=255)

                                                                                                                           gCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCg                         <  1:486745/149‑1 (MQ=255)

                                                                                                                             aaCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTc                       >  1:3469526/1‑149 (MQ=255)

                                                                                                                              aCATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCACCGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCa                      <  2:3170958/149‑1 (MQ=255)

                                                                                                                              aCATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCa                      >  2:3054847/1‑149 (MQ=255)

                                                                                                                                aTGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgc                    >  2:1741063/1‑149 (MQ=255)

                                                                                                                                aTGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgc                    >  2:2309020/1‑149 (MQ=255)

                                                                                                                                   gTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCt                 <  1:3433871/149‑1 (MQ=255)

                                                                                                                                      aCTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTAcc              >  1:3609735/1‑149 (MQ=255)

                                                                                                                                        tAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGt            <  2:2072617/149‑1 (MQ=255)

                                                                                                                                        tAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGt            >  2:2987640/1‑149 (MQ=255)

                                                                                                                                          aCCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGAAAACAGGTTATGGTAAATAAACGACAGCGCTACCTTGa          >  1:2168440/1‑149 (MQ=255)

                                                                                                                                              tATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGaaaaa      <  2:3972995/149‑1 (MQ=255)

                                                                                                                                               aTCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAg     >  1:3942594/1‑149 (MQ=255)

                                                                                                                                                  aaCACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGtct  <  2:3469526/149‑1 (MQ=255)

GATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGTCT  >  CP009273/2277597‑2277891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`