Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,289,043 C→A 100% W8L (TGG→TTG)  ccmD ← cytochrome c biogenesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,289,0430CA98.0% 834.4 / 5.9 264W8L (TGG→TTG) ccmDcytochrome c biogenesis protein
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (115/143);  total (118/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 265 total aligned reads displayed.

CACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAC  >  CP009273/2288897‑2289188
                                                                                                                                                  |                                                                                                                                                 
 aCGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAgg                                                                                                                                                >  1:2299036/1‑149 (MQ=255)
      cccGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCa                                                                                                                                           <  2:2521418/149‑1 (MQ=255)
      cccGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCa                                                                                                                                           <  1:1493438/149‑1 (MQ=255)
        cGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCaaa                                                                                                                                         >  1:3880931/1‑149 (MQ=255)
        cGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCaaa                                                                                                                                         <  1:2780330/149‑1 (MQ=255)
        cGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCaaa                                                                                                                                         <  1:2334717/149‑1 (MQ=255)
            gTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCa                                                                                                                                     <  1:895001/149‑1 (MQ=255)
            gTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCa                                                                                                                                     >  1:1458902/1‑149 (MQ=255)
               gcgcCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAggg                                                                                                                                  <  2:652361/149‑1 (MQ=255)
                cgccacgccaCGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAgggg                                                                                                                                 <  2:2433463/149‑1 (MQ=255)
                  ccacgccacgCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTc                                                                                                                               >  1:1002238/1‑149 (MQ=255)
                  ccacgccacgCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTc                                                                                                                               <  1:2162272/149‑1 (MQ=255)
                    acgccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCAt                                                                                                                             >  1:3800171/1‑149 (MQ=255)
                      gccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCAttt                                                                                                                           <  2:1093627/149‑1 (MQ=255)
                       ccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTa                                                                                                                          <  1:433516/149‑1 (MQ=255)
                          cgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACgg                                                                                                                       >  2:2847311/1‑149 (MQ=255)
                           gcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGc                                                                                                                      >  2:513071/1‑149 (MQ=255)
                             aGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCct                                                                                                                    <  2:2961191/149‑1 (MQ=255)
                              gAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCctc                                                                                                                   >  2:1978935/1‑149 (MQ=255)
                               aaTTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCctct                                                                                                                  <  1:666306/149‑1 (MQ=255)
                                aTTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCtt                                                                                                                 >  1:361341/1‑149 (MQ=255)
                                     gcgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAg                                                                                                            >  2:1564029/1‑149 (MQ=255)
                                       gATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTa                                                                                                          <  1:2915694/149‑1 (MQ=255)
                                       gATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTa                                                                                                          <  1:154632/149‑1 (MQ=255)
                                       gATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTa                                                                                                          >  2:2467128/1‑149 (MQ=255)
                                        aTGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTAt                                                                                                         >  1:3694643/1‑149 (MQ=255)
                                         tGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATc                                                                                                        <  1:975845/149‑1 (MQ=255)
                                           ttGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAg                                                                                                      >  2:3778942/1‑149 (MQ=255)
                                            tGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGt                                                                                                     <  1:1666400/149‑1 (MQ=255)
                                              cATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtc                                                                                                   <  1:3716911/149‑1 (MQ=255)
                                              cATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtc                                                                                                   <  2:3698396/149‑1 (MQ=255)
                                              cATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtc                                                                                                   <  1:3986441/149‑1 (MQ=255)
                                                tCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcac                                                                                                 >  1:627991/1‑149 (MQ=255)
                                                tCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcac                                                                                                 <  2:3542901/149‑1 (MQ=255)
                                                    cGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcactcac                                                                                             <  2:4040167/149‑1 (MQ=255)
                                                     gAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCAcc                                                                                            <  1:3166623/149‑1 (MQ=255)
                                                         gCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacg                                                                                        >  2:3963902/1‑149 (MQ=255)
                                                          cACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacgg                                                                                       >  1:409432/1‑149 (MQ=255)
                                                           aCGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacgga                                                                                      >  1:967769/1‑149 (MQ=255)
                                                              aCCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacgg                                                                                   <  2:2873081/149‑1 (MQ=255)
                                                               ccAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGc                                                                                  >  2:1669718/1‑149 (MQ=255)
                                                                 aaaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGt                                                                                <  2:160082/149‑1 (MQ=255)
                                                                  aaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                               <  2:2176904/149‑1 (MQ=255)
                                                                  aaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                               <  2:360994/149‑1 (MQ=255)
                                                                   aaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGttt                                                                              <  1:2820219/149‑1 (MQ=255)
                                                                    accaacAGCGGAATAACGGTCATCACCACAGACAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAATAATTCATTACAGGAAGCATATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtttt                                                                             <  2:1384705/149‑1 (MQ=255)
                                                                     ccaccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGttttt                                                                            >  1:2696473/1‑149 (MQ=255)
                                                                      caccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTc                                                                           <  1:3548845/149‑1 (MQ=255)
                                                                         caGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCAt                                                                        <  1:2255026/149‑1 (MQ=255)
                                                                           gCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCa                                                                      <  2:2922704/149‑1 (MQ=255)
                                                                            cGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAg                                                                     >  2:2852034/1‑149 (MQ=255)
                                                                             ggAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGc                                                                    <  2:517409/149‑1 (MQ=255)
                                                                              gAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCa                                                                   >  1:1033006/1‑149 (MQ=255)
                                                                                   aCGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATc                                                              <  2:3431445/149‑1 (MQ=255)
                                                                                   aCGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATc                                                              >  1:3408107/1‑149 (MQ=255)
                                                                                      gTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCaaa                                                           <  2:296830/149‑1 (MQ=255)
                                                                                       tcatcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAt                                                          <  2:190542/149‑1 (MQ=255)
                                                                                       tcatcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAt                                                          >  2:412952/1‑149 (MQ=255)
                                                                                         atcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTa                                                        <  2:2140479/149‑1 (MQ=255)
                                                                                          tcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAc                                                       <  1:1259068/149‑1 (MQ=255)
                                                                                          tcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAc                                                       >  1:3335096/1‑149 (MQ=255)
                                                                                            accaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgc                                                     <  2:3215251/149‑1 (MQ=255)
                                                                                             ccaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgca                                                    <  2:3082421/149‑1 (MQ=255)
                                                                                             ccaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgca                                                    >  2:739480/1‑149 (MQ=255)
                                                                                             ccaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgca                                                    >  2:2598669/1‑149 (MQ=255)
                                                                                             ccaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgca                                                    >  1:1156575/1‑149 (MQ=255)
                                                                                               accGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatc                                                  <  1:2988096/149‑1 (MQ=255)
                                                                                                  gccagccaGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgc                                               <  2:1296783/149‑1 (MQ=255)
                                                                                                   ccagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgca                                              <  1:1829156/149‑1 (MQ=255)
                                                                                                   ccagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgca                                              <  1:1158937/149‑1 (MQ=255)
                                                                                                   ccagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgca                                              <  2:2731714/149‑1 (MQ=255)
                                                                                                     agacagACAAAAAAGGCGTTACCGCCCATTGCGTAAAATTCATTCAAGGAAGCTAATGCAGGGGTAATTTACGGCATCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATAAGCAAAATCAAATTAcgcatccgcatc                                            <  2:1519035/146‑1 (MQ=255)
                                                                                                         agACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcg                                        >  2:1371390/1‑149 (MQ=255)
                                                                                                          gACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgt                                       >  2:3156363/1‑149 (MQ=255)
                                                                                                            cAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtca                                     <  2:2653531/149‑1 (MQ=255)
                                                                                                              aaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCAATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagc                                   >  2:174834/1‑149 (MQ=255)
                                                                                                              aaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATATACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcaccgacagc                                   >  2:1466157/1‑149 (MQ=255)
                                                                                                               aaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagcg                                  >  1:359694/1‑149 (MQ=255)
                                                                                                                aaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATcagcgtcagcgt                                 <  2:526830/149‑1 (MQ=255)
                                                                                                                 aaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTg                                <  2:578824/149‑1 (MQ=255)
                                                                                                                   ggCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGc                              <  2:3387258/149‑1 (MQ=255)
                                                                                                                    gCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCa                             <  2:3984240/149‑1 (MQ=255)
                                                                                                                       tAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGAc                          <  1:501257/149‑1 (MQ=255)
                                                                                                                         aCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAg                        >  1:3710448/1‑149 (MQ=255)
                                                                                                                             cccATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGc                    <  2:2299036/149‑1 (MQ=255)
                                                                                                                              ccATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATATACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCa                   >  2:1028502/1‑149 (MQ=255)
                                                                                                                               cATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAg                  >  1:2402604/1‑149 (MQ=255)
                                                                                                                                 ttGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGa                >  1:2613038/1‑149 (MQ=255)
                                                                                                                                     gAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGcc            <  2:627972/149‑1 (MQ=255)
                                                                                                                                      aaaaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCa           >  1:3485973/1‑149 (MQ=255)
                                                                                                                                       aaaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaa          >  1:259798/1‑149 (MQ=255)
                                                                                                                                        aaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaa         >  2:1257572/1‑149 (MQ=255)
                                                                                                                                        aaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaa         >  2:3344848/1‑149 (MQ=255)
                                                                                                                                          attcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaaaa       <  2:942933/149‑1 (MQ=255)
                                                                                                                                           ttcattcaAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAAt      >  2:876696/1‑149 (MQ=255)
                                                                                                                                              attcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGa   <  1:2847314/149‑1 (MQ=255)
                                                                                                                                              attcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGa   >  1:2078546/1‑149 (MQ=255)
                                                                                                                                               ttcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAc  >  1:988674/1‑149 (MQ=255)
                                                                                                                                               ttcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAc  >  2:2816126/1‑149 (MQ=255)
                                                                                                                                               ttcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAc  >  2:2122169/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGAC  >  CP009273/2288897‑2289188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑