| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,322,609 | C→A | 100% | W223L (TGG→TTG) | yfaQ ← | tandem DUF2300 domain protein, putative host defense protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,322,609 | 0 | C | A | 96.6% | 754.0 / 13.0 | 246 | W223L (TGG→TTG) | yfaQ | tandem DUF2300 domain protein, putative host defense protein |
| Reads supporting (aligned to +/- strand): ref base C (4/5); new base A (104/133); total (108/138) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 248 total aligned reads displayed.
CTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCAC > CP009273/2322463‑2322754 | cTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCaac > 1:2213268/1‑149 (MQ=255) gtgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAg < 1:1054785/149‑1 (MQ=255) gATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCg > 2:2249076/1‑149 (MQ=255) gATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCg < 2:2113336/149‑1 (MQ=255) aTTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCgg > 1:628303/1‑149 (MQ=255) aTTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCgg > 1:4105877/1‑149 (MQ=255) aTTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCgg > 1:232188/1‑149 (MQ=255) tttCTTCAGCGGATAGCGTGCAAACAGCTCCACCTCTACACATTGCCCACTCGCAACCTGCGTCGGCACTGGCAAAACCCGATTTAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGgtg < 2:1275862/149‑1 (MQ=255) tttCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGgtg < 2:288059/149‑1 (MQ=255) ttcttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGgtgt < 2:3143350/149‑1 (MQ=255) ttcttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGgtgt < 2:3143356/149‑1 (MQ=255) tcttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTa < 2:2386575/149‑1 (MQ=255) tcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCa > 2:1985563/1‑149 (MQ=255) ggATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGt < 1:1005985/149‑1 (MQ=255) ggATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGt < 2:2746599/149‑1 (MQ=255) ggATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGt < 1:835851/149‑1 (MQ=255) ggATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGt > 1:1346874/1‑149 (MQ=255) gATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGtt < 2:3286476/149‑1 (MQ=255) gATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGtt < 2:3722616/149‑1 (MQ=255) gATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGtt < 2:1783118/149‑1 (MQ=255) aTAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTa < 2:1022570/149‑1 (MQ=255) aGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAc < 1:1681985/149‑1 (MQ=255) cGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAcca < 2:1339276/149‑1 (MQ=255) cGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAcca < 1:2940474/149‑1 (MQ=255) cGGGCAAACAGCTCCACCTCTACACAGTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAcca < 2:2434355/149‑1 (MQ=255) gggCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAccag > 1:1668827/1‑149 (MQ=255) gCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAccagcc > 2:4036815/1‑149 (MQ=255) aaaCAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAccagccag > 1:1420434/1‑149 (MQ=255) aaCAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACcagccagc < 2:2186378/149‑1 (MQ=255) aaCAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACcagccagc > 1:1960894/1‑149 (MQ=255) aGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCaaa > 1:3595513/1‑149 (MQ=255) aGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCaaa > 1:3734953/1‑149 (MQ=255) gCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCaaaa > 1:121496/1‑149 (MQ=255) ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCAccc < 1:240475/149‑1 (MQ=255) ctACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGa < 2:1778400/149‑1 (MQ=255) ctACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGa < 2:2474654/149‑1 (MQ=255) ctACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGa < 2:3880274/149‑1 (MQ=255) ctACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGa < 2:3970025/149‑1 (MQ=255) tACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGAc < 1:2839014/149‑1 (MQ=255) caTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCAGACGGAt > 1:2782047/1‑149 (MQ=255) aTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAtt < 2:2717884/149‑1 (MQ=255) gCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTcc < 1:2027277/149‑1 (MQ=255) cccACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCt < 2:1998819/149‑1 (MQ=255) cACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑t < 2:2076182/149‑1 (MQ=255) aCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTCCCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑tt > 2:3344915/1‑149 (MQ=255) aCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑tt < 1:3529692/149‑1 (MQ=255) aCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑tt < 1:2689018/149‑1 (MQ=255) gCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTatc < 2:26224/149‑1 (MQ=255) aCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTAGCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTatcatc > 1:1482982/1‑149 (MQ=255) aCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTatcatc < 1:3965710/149‑1 (MQ=255) ccTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCg > 2:1497041/1‑149 (MQ=255) cttcGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCgg > 1:1972050/4‑149 (MQ=255) cttcGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCgg > 1:1494193/4‑149 (MQ=255) tGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGc > 1:2900215/1‑149 (MQ=255) tCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAAc > 2:2567073/1‑149 (MQ=255) tCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAAc > 2:1738605/1‑149 (MQ=255) gCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCACACCAg > 2:2784613/1‑149 (MQ=255) gCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAg < 1:1287381/149‑1 (MQ=255) gCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAg < 1:4082298/149‑1 (MQ=255) cTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTc < 1:3947296/149‑1 (MQ=255) tGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTcc < 2:4057094/149‑1 (MQ=255) ggCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCa < 2:1696015/149‑1 (MQ=255) aaaaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGt > 1:985514/1‑149 (MQ=255) aaaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGtt > 2:1203581/1‑149 (MQ=255) aaaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGtt < 2:1650533/149‑1 (MQ=255) aaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGttt < 2:74806/149‑1 (MQ=255) ccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCa < 1:3225458/149‑1 (MQ=255) aTTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTTATCATCGGCAAACCAGCTCCAGGTTTTCAcc > 1:3670484/1‑149 (MQ=255) aTTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCAccc > 2:2178535/1‑149 (MQ=255) aTTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCAccc > 2:3578383/1‑149 (MQ=255) aGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTAc > 1:245336/1‑149 (MQ=255) cACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGTAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTAccc < 2:2275621/149‑1 (MQ=255) cGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGAc < 1:878874/149‑1 (MQ=255) gCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTAcc > 2:1256907/1‑149 (MQ=255) cATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCg > 1:1042753/1‑149 (MQ=255) aTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGNAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGa > 1:2084815/1‑149 (MQ=255) aTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGa < 2:669150/149‑1 (MQ=255) aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTCCCGAGc > 2:2437232/1‑149 (MQ=255) aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGc < 2:2022299/149‑1 (MQ=255) aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGc < 2:2451859/149‑1 (MQ=255) ttAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGAt > 2:1858340/1‑149 (MQ=255) aGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACAGAGCAGGGATGc > 2:1032482/1‑149 (MQ=255) gTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCg > 1:910492/1‑149 (MQ=255) aCCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGac < 1:3695530/149‑1 (MQ=255) cGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGacac < 1:1270338/149‑1 (MQ=255) cGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGacac > 1:2775077/1‑149 (MQ=255) gCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAttt < 1:1282759/149‑1 (MQ=255) gCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAttt < 2:3225240/149‑1 (MQ=255) gCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCt < 1:3829282/149‑1 (MQ=255) gCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCt < 2:2457094/149‑1 (MQ=255) cACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCg > 1:1901487/1‑149 (MQ=255) aCTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGt > 1:1808106/1‑149 (MQ=255) tGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTcc < 2:317010/149‑1 (MQ=255) gCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCa < 1:475762/149‑1 (MQ=255) gCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCa < 1:852974/149‑1 (MQ=255) gCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCa < 1:2543553/149‑1 (MQ=255) ccAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAg > 1:3071407/1‑149 (MQ=255) tAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcacc > 2:2518479/1‑149 (MQ=255) tAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcacc > 2:2516986/1‑149 (MQ=255) aaCCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcacca > 2:1817733/1‑149 (MQ=255) | CTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTG‑TTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCAC > CP009273/2322463‑2322754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |