Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,359,673 G→T 100% M89I (ATG→ATT)  arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,359,6730GT99.6% 928.4 / ‑4.5 279M89I (ATG→ATT) arnBuridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (163/115);  total (163/116)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 286 total aligned reads displayed.

GCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTG  >  CP009273/2359527‑2359818
                                                                                                                                                  |                                                                                                                                                 
gCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAAtattat                                                                                                                                                 >  2:369136/1‑149 (MQ=255)
 cTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATattatt                                                                                                                                                >  2:1972695/1‑149 (MQ=255)
  tttttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTAttt                                                                                                                                               >  2:3320022/1‑149 (MQ=255)
   ttttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTc                                                                                                                                              <  1:3368922/149‑1 (MQ=255)
       gCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttg                                                                                                                                          >  1:1376837/1‑149 (MQ=255)
       gCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttg                                                                                                                                          >  1:1960497/1‑149 (MQ=255)
          aGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttgttg                                                                                                                                       <  2:3396648/149‑1 (MQ=255)
           gTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTgg                                                                                                                                      >  2:560728/1‑149 (MQ=255)
                  ggAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAAc                                                                                                                               >  2:449120/1‑149 (MQ=255)
                  ggAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTAATTGTTGGGTGCAAc                                                                                                                               >  2:3095002/1‑149 (MQ=255)
                   gAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACg                                                                                                                              >  1:1264059/1‑149 (MQ=255)
                       tCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCgg                                                                                                                          >  1:2145531/1‑149 (MQ=255)
                         aGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTa                                                                                                                        <  1:3551232/149‑1 (MQ=255)
                          gCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTaa                                                                                                                       >  2:2403070/1‑149 (MQ=255)
                          gCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTaa                                                                                                                       <  2:2023824/149‑1 (MQ=255)
                          gCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTaa                                                                                                                       <  2:4060985/149‑1 (MQ=255)
                           cATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAt                                                                                                                      <  2:1779562/149‑1 (MQ=255)
                           cATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAt                                                                                                                      <  1:1741756/149‑1 (MQ=255)
                            aTGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAtg                                                                                                                     >  1:2225557/1‑149 (MQ=255)
                            aTGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAtg                                                                                                                     <  1:2493944/149‑1 (MQ=255)
                            aTGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAtg                                                                                                                     >  1:2178604/1‑149 (MQ=255)
                                 aTCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGa                                                                                                                <  1:1149860/149‑1 (MQ=255)
                                    gcgGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGt                                                                                                             <  2:1013742/149‑1 (MQ=255)
                                    gcgGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGt                                                                                                             <  2:691498/149‑1 (MQ=255)
                                        tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         <  2:2612040/149‑1 (MQ=255)
                                        tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         <  2:94325/149‑1 (MQ=255)
                                        tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         <  2:1637909/149‑1 (MQ=255)
                                        tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         >  1:445243/1‑149 (MQ=255)
                                        tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         <  1:3875807/149‑1 (MQ=255)
                                        tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         <  1:3873268/149‑1 (MQ=255)
                                        tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         <  1:3625632/149‑1 (MQ=255)
                                        tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         <  1:2340722/149‑1 (MQ=255)
                                         cAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAcc                                                                                                        <  1:1196598/149‑1 (MQ=255)
                                           gTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcgc                                                                                                      >  1:2544512/1‑149 (MQ=255)
                                           gTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCATTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcgc                                                                                                      >  1:768167/1‑149 (MQ=255)
                                                  cACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGc                                                                                               <  2:1985387/149‑1 (MQ=255)
                                                  cACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGc                                                                                               <  2:1296822/149‑1 (MQ=255)
                                                  cACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGc                                                                                               >  2:3900378/1‑149 (MQ=255)
                                                     cgccgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGa                                                                                            <  1:1641193/149‑1 (MQ=255)
                                                     cgccgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGa                                                                                            >  2:2149810/1‑149 (MQ=255)
                                                     cgccgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGa                                                                                            >  2:218987/1‑149 (MQ=255)
                                                       ccgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATg                                                                                          >  2:3139333/1‑149 (MQ=255)
                                                          gAATGCATATCACGCGAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTc                                                                                       <  2:2100097/149‑1 (MQ=255)
                                                           aaTGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCa                                                                                      <  2:3671254/149‑1 (MQ=255)
                                                           aaTGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCa                                                                                      >  2:664549/1‑149 (MQ=255)
                                                           aaTGCATATCACGCTAATGGCGTGGAAAATTGGCAAGGGCGATGAAGTGATTACTCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATATCCTTGTTGGGTGCAACTCCGGTAATGCTGGATGTCGACCGCGATACTCTGATGGTCa                                                                                      >  2:1583623/1‑149 (MQ=255)
                                                            aTGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCAc                                                                                     <  2:2103280/149‑1 (MQ=255)
                                                             tGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACg                                                                                    >  2:2172774/1‑149 (MQ=255)
                                                                  atCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTCATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGa                                                                               >  1:656493/1‑149 (MQ=255)
                                                                   tCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGaa                                                                              >  1:270335/1‑149 (MQ=255)
                                                                   tCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGaa                                                                              >  1:3912319/1‑149 (MQ=255)
                                                                      cGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCt                                                                           <  1:68540/149‑1 (MQ=255)
                                                                         tAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATc                                                                        >  2:3070548/1‑149 (MQ=255)
                                                                          aaTGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCg                                                                       <  1:1993704/149‑1 (MQ=255)
                                                                           aTGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGa                                                                      >  1:1519832/1‑149 (MQ=255)
                                                                             ggCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGt                                                                    >  1:3299178/1‑149 (MQ=255)
                                                                                gTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAg                                                                 >  1:2973444/1‑149 (MQ=255)
                                                                                  tGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGcc                                                               >  1:535084/1‑149 (MQ=255)
                                                                                   gAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCa                                                              >  1:1129594/1‑149 (MQ=255)
                                                                                    aaaaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCAt                                                             >  1:3724093/1‑149 (MQ=255)
                                                                                    aaaaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCAt                                                             >  2:2860917/1‑149 (MQ=255)
                                                                                      aaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTa                                                           <  1:3684443/149‑1 (MQ=255)
                                                                                            cAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCAc                                                     <  1:923340/149‑1 (MQ=255)
                                                                                              aGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGc                                                   >  2:1289738/1‑149 (MQ=255)
                                                                                              aGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGc                                                   >  1:165693/1‑149 (MQ=255)
                                                                                               gggCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCa                                                  <  2:31294/149‑1 (MQ=255)
                                                                                                ggCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCAc                                                 <  2:2278572/149‑1 (MQ=255)
                                                                                                   gATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaa                                              >  1:2362458/1‑149 (MQ=255)
                                                                                                   gATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaa                                              >  1:1292849/1‑149 (MQ=255)
                                                                                                    aTGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaaa                                             >  2:1931405/1‑149 (MQ=255)
                                                                                                    aTGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaaa                                             >  2:3840028/1‑149 (MQ=255)
                                                                                                     tGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAg                                            >  1:3243351/1‑149 (MQ=255)
                                                                                                     tGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAg                                            <  2:55343/149‑1 (MQ=255)
                                                                                                     tGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATATCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAg                                            >  2:84277/1‑149 (MQ=255)
                                                                                                     tGAAGTGATTACGCCGTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAg                                            <  2:55319/149‑1 (MQ=255)
                                                                                                      gAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGc                                           >  2:1165592/1‑149 (MQ=255)
                                                                                                       aaGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGcc                                          >  2:2779147/1‑149 (MQ=255)
                                                                                                        aGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCca                                         >  2:1451845/1‑149 (MQ=255)
                                                                                                              tACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTc                                   <  2:3465524/149‑1 (MQ=255)
                                                                                                                cGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCg                                 <  2:3571301/149‑1 (MQ=255)
                                                                                                                  ccTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGt                               >  1:462630/1‑149 (MQ=255)
                                                                                                                  ccTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGt                               <  1:3350297/149‑1 (MQ=255)
                                                                                                                    ttCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCcatc                                       >  1:556925/1‑139 (MQ=255)
                                                                                                                    ttCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGc                             >  2:139971/1‑149 (MQ=255)
                                                                                                                     tCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCa                            <  2:3110687/149‑1 (MQ=255)
                                                                                                                       ccTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGt                               >  2:2364801/1‑144 (MQ=255)
                                                                                                                          gACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATg                       <  1:3099300/149‑1 (MQ=255)
                                                                                                                           aCCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGc                      <  2:2985423/149‑1 (MQ=255)
                                                                                                                           aCCTGGGTTTCAACCCTCAATATTATGTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGc                      <  1:816924/149‑1 (MQ=255)
                                                                                                                            ccTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGcc                     >  1:2580045/1‑149 (MQ=255)
                                                                                                                             cTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCg                    <  1:3445021/149‑1 (MQ=255)
                                                                                                                                ggTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTg                 >  1:2771279/1‑149 (MQ=255)
                                                                                                                                 gTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTgc                >  2:916875/1‑149 (MQ=255)
                                                                                                                                     cAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCca            >  2:3712885/1‑149 (MQ=255)
                                                                                                                                      aaCCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcag           >  2:1861235/1‑149 (MQ=255)
                                                                                                                                       aCCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagc          >  1:1838163/1‑149 (MQ=255)
                                                                                                                                        cccTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagca         >  2:430730/1‑149 (MQ=255)
                                                                                                                                        cccTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagca         >  1:2608458/1‑149 (MQ=255)
                                                                                                                                           tCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGat      <  2:2575541/149‑1 (MQ=255)
                                                                                                                                             aaTATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGatat    >  1:783814/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTG  >  CP009273/2359527‑2359818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑