Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 233,829 C→A 39.7% S3* (TCA→TAA)  yafT → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273233,8290CA39.7% 233.6 / 220.3 212S3* (TCA→TAA) yafTlipoprotein
Reads supporting (aligned to +/- strand):  ref base C (69/59);  new base A (45/39);  total (114/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

Only 100 of 212 total aligned reads displayed.

GAATAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATT  >  CP009273/233684‑233975
                                                                                                                                                 |                                                                                                                                                  
     aaTTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGc                                                                                                                                            <  1:702670/149‑1 (MQ=255)
     aaTTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAAGTAAAAAAAGc                                                                                                                                            >  1:1657247/1‑149 (MQ=255)
      aTTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCt                                                                                                                                           <  1:1054088/149‑1 (MQ=255)
       tttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCtt                                                                                                                                          >  2:147869/1‑149 (MQ=255)
       tttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCtt                                                                                                                                          <  1:2509855/149‑1 (MQ=255)
        ttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCttt                                                                                                                                         >  1:3973047/1‑149 (MQ=255)
          atatTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTttgt                                                                                                                                       <  1:3134962/149‑1 (MQ=255)
           tatTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTttgtt                                                                                                                                      >  1:3756265/1‑149 (MQ=255)
             ttGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGc                                                                                                                                    >  2:589048/1‑149 (MQ=255)
              tGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCa                                                                                                                                   <  2:1139977/149‑1 (MQ=255)
              cgATTAAATGCATGTATCTTTCACATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATACTTAGAGTCCGTTATCAATGCAAAAGATTCGAATCCTTATGACAGGCGAGGGAGTGTCCAAATATGAATTCAAAAAAGCTTTGTTGCa                                                                                                                                   <  2:2171757/148‑1 (MQ=255)
                aTTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCata                                                                                                                                 <  1:2790521/149‑1 (MQ=255)
                aTTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCata                                                                                                                                 <  1:24685/149‑1 (MQ=255)
                aTTAAATGAATGTATATTTCAAATTGATTTTGTTTGGTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGCGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCata                                                                                                                                 >  2:272954/1‑149 (MQ=255)
                 ttAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCatat                                                                                                                                >  2:2107645/1‑149 (MQ=255)
                  tAAATGAATGCATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATAtg                                                                                                                               <  1:1305492/149‑1 (MQ=255)
                   aaaTGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATAtgt                                                                                                                              <  2:1687121/149‑1 (MQ=255)
                     atgaatgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATAtgtgt                                                                                                                            >  2:915323/1‑149 (MQ=255)
                     atgaatgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGATTTGTTGCATAtgtgt                                                                                                                            >  2:121142/1‑149 (MQ=255)
                      tgaatgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATAtgtgtg                                                                                                                           <  1:4091940/149‑1 (MQ=255)
                         atgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTa                                                                                                                        >  2:3060519/1‑149 (MQ=255)
                         atgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTAATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTa                                                                                                                        >  2:410835/1‑149 (MQ=255)
                           gTATATTTCAAATTTATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTAtt                                                                                                                      <  2:3492841/149‑1 (MQ=255)
                           gTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTAtt                                                                                                                      <  2:1624008/149‑1 (MQ=255)
                           gTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTAtt                                                                                                                      >  1:1303978/1‑149 (MQ=255)
                           gTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTAtt                                                                                                                      >  1:852073/1‑149 (MQ=255)
                             atatTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATtct                                                                                                                    <  2:1904562/149‑1 (MQ=255)
                                tttCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCgc                                                                                                                 <  2:1174915/149‑1 (MQ=255)
                                   cAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCgctgc                                                                                                              >  2:3090158/1‑149 (MQ=255)
                                     aaTTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCtt                                                                                                            >  2:1811692/1‑149 (MQ=255)
                                      aTTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTg                                                                                                           >  1:1274312/1‑149 (MQ=255)
                                           ttttGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGa                                                                                                      >  1:3852775/1‑149 (MQ=255)
                                           ttttGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGa                                                                                                      <  2:2554420/149‑1 (MQ=255)
                                              tgtttgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGAtgt                                                                                                   <  2:2138777/149‑1 (MQ=255)
                                              tgtttgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGAtgt                                                                                                   <  2:59731/149‑1 (MQ=255)
                                                tttgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGc                                                                                                 <  1:772010/149‑1 (MQ=255)
                                                tttgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGc                                                                                                 >  2:3349729/1‑149 (MQ=255)
                                                  tgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCct                                                                                               >  1:1172844/1‑149 (MQ=255)
                                                   gttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCctc                                                                                              <  1:1987703/149‑1 (MQ=255)
                                                    ttattaTTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCctct                                                                                             <  2:526038/149‑1 (MQ=255)
                                                    ttattaTTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCctct                                                                                             >  1:681274/1‑149 (MQ=255)
                                                     tattatTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTg                                                                                            <  1:3490511/149‑1 (MQ=255)
                                                       ttattTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGaa                                                                                          >  2:2660777/1‑149 (MQ=255)
                                                       ttattTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGaa                                                                                          >  2:1635985/1‑149 (MQ=255)
                                                        tattTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAt                                                                                         >  1:2342386/1‑149 (MQ=255)
                                                             aaGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtcttct                                                                                    >  2:81722/1‑149 (MQ=255)
                                                               gTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTAt                                                                                  >  2:2230232/1‑149 (MQ=255)
                                                               gTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTAt                                                                                  >  1:2613604/1‑149 (MQ=255)
                                                                 gAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtg                                                                                <  2:3644524/149‑1 (MQ=255)
                                                                   ggTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtgat                                                                              >  1:1731784/1‑149 (MQ=255)
                                                                   ggTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtgat                                                                              >  1:1015473/1‑149 (MQ=255)
                                                                       tataATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGaaa                                                                          >  1:425177/1‑149 (MQ=255)
                                                                         taATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaag                                                                        <  1:961179/149‑1 (MQ=255)
                                                                          aaTTAGAGTCCGTTATCAATTCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaaga                                                                       <  1:3222834/149‑1 (MQ=255)
                                                                          aaTTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaaga                                                                       <  2:2527171/149‑1 (MQ=255)
                                                                           aTTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaa                                                                      >  2:3814846/1‑149 (MQ=255)
                                                                            ttAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaag                                                                     >  1:3782441/1‑149 (MQ=255)
                                                                            ttAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaag                                                                     <  1:589048/149‑1 (MQ=255)
                                                                               gagTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaa                                                                  <  1:2836977/149‑1 (MQ=255)
                                                                               gagTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaa                                                                  >  2:3721346/1‑149 (MQ=255)
                                                                                agTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaa                                                                 <  2:3855500/149‑1 (MQ=255)
                                                                                agTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaa                                                                 >  1:279348/1‑149 (MQ=255)
                                                                                 gTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaa                                                                >  2:1762106/1‑149 (MQ=255)
                                                                                  tCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaaa                                                               <  1:3184279/149‑1 (MQ=255)
                                                                                  tCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaaa                                                               <  1:2230232/149‑1 (MQ=255)
                                                                                  tCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaaa                                                               >  1:2564462/1‑149 (MQ=255)
                                                                                    cGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGc                                                             <  1:1509490/149‑1 (MQ=255)
                                                                                     gTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCt                                                            >  1:2162316/1‑149 (MQ=255)
                                                                                        aTCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCaa                                                         <  1:645586/149‑1 (MQ=255)
                                                                                         tCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAg                                                        <  1:400082/149‑1 (MQ=255)
                                                                                          cacttcTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGt                                                       <  2:2463891/144‑1 (MQ=255)
                                                                                           aaTGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTc                                                      <  1:3642392/149‑1 (MQ=255)
                                                                                            aTGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTca                                                     >  1:1559755/1‑149 (MQ=255)
                                                                                             tGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAATAAAAAAGCTCAAGTcac                                                    >  1:3457834/1‑149 (MQ=255)
                                                                                             tGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTcac                                                    <  2:865028/149‑1 (MQ=255)
                                                                                              gCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGACGAAAAAAGCTCAAGTcaca                                                   >  2:103180/1‑149 (MQ=255)
                                                                                                 aaaTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACaa                                                >  1:497339/1‑149 (MQ=255)
                                                                                                 aaaTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACaa                                                >  2:158039/1‑149 (MQ=255)
                                                                                                    tatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGt                                             <  2:541229/149‑1 (MQ=255)
                                                                                                           aTCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTAGTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATta                                      <  2:186100/149‑1 (MQ=255)
                                                                                                           aTCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATta                                      <  2:1953799/149‑1 (MQ=255)
                                                                                                             cATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaat                                    >  1:3579621/1‑149 (MQ=255)
                                                                                                               ttATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaataa                                  >  2:1266433/1‑149 (MQ=255)
                                                                                                                 aTGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATaaaa                                <  1:1765728/149‑1 (MQ=255)
                                                                                                                  tGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATaaaaa                               <  1:3264389/149‑1 (MQ=255)
                                                                                                                  tGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATaaaaa                               <  2:2613604/149‑1 (MQ=255)
                                                                                                                   gACAGGCGAGGGAGTGTCCAAGTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAac                              >  2:892752/1‑149 (MQ=255)
                                                                                                                     cAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAacac                            >  2:2884933/1‑149 (MQ=255)
                                                                                                                       ggCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTc                          >  1:3365110/1‑149 (MQ=255)
                                                                                                                           aGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCa                      >  2:2369491/1‑149 (MQ=255)
                                                                                                                           aGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCa                      >  2:2366632/1‑149 (MQ=255)
                                                                                                                            gggAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAg                     >  1:2877303/1‑149 (MQ=255)
                                                                                                                                 tgtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACt                >  2:667257/1‑149 (MQ=255)
                                                                                                                                 tgtCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACt                >  2:478467/1‑149 (MQ=255)
                                                                                                                                     cAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgaca            >  1:210331/1‑149 (MQ=255)
                                                                                                                                      aaTTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacag           <  1:3060519/149‑1 (MQ=255)
                                                                                                                                       aTTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacaga          <  1:272954/149‑1 (MQ=255)
                                                                                                                                               ttCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGAtt  <  2:2823427/149‑1 (MQ=255)
                                                                                                                                               ttCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGAtt  <  2:761264/149‑1 (MQ=255)
                                                                                                                                               ttCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGAtt  <  1:2403871/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GAATAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATT  >  CP009273/233684‑233975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑