Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,376,959 C→T 100% N256N (AAC→AAT)  elaD → protease, capable of cleaving an AMC‑ubiquitin model substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,376,9590CT98.8% 775.5 / ‑0.1 239N256N (AAC→AAT) elaDprotease, capable of cleaving an AMC‑ubiquitin model substrate
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base T (116/120);  total (119/120)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

AATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATC  >  CP009273/2376813‑2377104
                                                                                                                                                  |                                                                                                                                                 
 aTGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATattcaattca                                                                                                                                                >  2:3170879/1‑149 (MQ=255)
     aaaTATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGc                                                                                                                                            >  2:1446537/1‑149 (MQ=255)
     aaaTATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGc                                                                                                                                            >  2:3495483/1‑149 (MQ=255)
             acagCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTa                                                                                                                                    <  2:2573329/147‑1 (MQ=255)
              gagCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAg                                                                                                                                   <  2:754888/149‑1 (MQ=255)
              gagCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAg                                                                                                                                   >  1:3251843/1‑149 (MQ=255)
              gagCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAg                                                                                                                                   >  1:1866536/1‑149 (MQ=255)
              gagCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAg                                                                                                                                   >  1:3906748/1‑149 (MQ=255)
               agCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAgg                                                                                                                                  >  2:761966/1‑149 (MQ=255)
                 cGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCt                                                                                                                                <  2:3604346/149‑1 (MQ=255)
                    tCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATg                                                                                                                             >  2:1976724/1‑149 (MQ=255)
                     ccATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGa                                                                                                                            <  2:1281397/149‑1 (MQ=255)
                      catcatGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGAt                                                                                                                           <  1:4008863/149‑1 (MQ=255)
                            ggCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGaa                                                                                                                     <  2:240613/149‑1 (MQ=255)
                            ggCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGaa                                                                                                                     >  1:1842237/1‑149 (MQ=255)
                                gAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAAtt                                                                                                                 >  1:2011028/1‑149 (MQ=255)
                                gAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACGAGGCTATGATAAAGAAAAtt                                                                                                                 <  1:3990893/149‑1 (MQ=255)
                                    gAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACt                                                                                                             <  1:1553947/149‑1 (MQ=255)
                                     aaGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACtt                                                                                                            <  2:1841761/149‑1 (MQ=255)
                                      aGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTa                                                                                                           >  1:1746380/1‑149 (MQ=255)
                                       gTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTaa                                                                                                          <  2:1392013/149‑1 (MQ=255)
                                             ttATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCgtgt                                                                                                    >  2:1073678/1‑149 (MQ=255)
                                                   cccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTaa                                                                                              <  1:720444/149‑1 (MQ=255)
                                                    ccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAAt                                                                                             <  1:2143370/149‑1 (MQ=255)
                                                    ccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAAt                                                                                             >  1:1478753/1‑149 (MQ=255)
                                                    ccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAAt                                                                                             >  1:1083720/1‑149 (MQ=255)
                                                     cATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAACTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATa                                                                                            <  2:3107354/149‑1 (MQ=255)
                                                        aaaTACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGtt                                                                                         <  2:363964/149‑1 (MQ=255)
                                                            aCTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTaa                                                                                     <  1:627441/149‑1 (MQ=255)
                                                             cTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAAt                                                                                    <  2:1746380/149‑1 (MQ=255)
                                                               ggCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTc                                                                                  <  2:2519738/149‑1 (MQ=255)
                                                                 cGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTg                                                                                <  1:1156581/149‑1 (MQ=255)
                                                                    acacTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAAc                                                                             <  1:2127792/149‑1 (MQ=255)
                                                                    acacTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAAc                                                                             <  1:1294071/149‑1 (MQ=255)
                                                                     cacTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACt                                                                            <  2:2451594/149‑1 (MQ=255)
                                                                     cacTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACt                                                                            >  2:2741792/1‑149 (MQ=255)
                                                                      acTTGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATACAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTc                                                                           <  1:1498824/149‑1 (MQ=255)
                                                                      acTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTc                                                                           >  1:2695531/1‑149 (MQ=255)
                                                                       cTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCa                                                                          >  1:1769945/1‑149 (MQ=255)
                                                                        tGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCAt                                                                         <  2:281516/149‑1 (MQ=255)
                                                                          gATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATgg                                                                       >  1:907973/1‑149 (MQ=255)
                                                                              ttAGTTTCGCTACAGAAAATAGTAAAT‑AAAAAAATAATAAAATTCAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTAACATAAGCGTGTCATTAATAGTTTTAAATCTTTACTAATGGGAGaa                                                                  >  2:1611536/1‑149 (MQ=255)
                                                                                aGTTTCGCTACAGAAAATATTAAATAAAAAAAATAATAAACTACAAATAAAATGAGTCATATTAAATTCATTTCGTGCACTAGGCTATGATAAAGAAAATTCACTAAAGCGTGTCATTAAAAGTTTTAATTCTGAACTCATGGGAGaaa                                                                 >  1:1577527/1‑149 (MQ=255)
                                                                                aGTTTCGATACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGaaa                                                                 <  2:896155/149‑1 (MQ=255)
                                                                                  tttCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATg                                                               >  2:2177724/1‑149 (MQ=255)
                                                                                   ttCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGt                                                              <  2:1136447/149‑1 (MQ=255)
                                                                                      gCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGa                                                           <  1:3170879/149‑1 (MQ=255)
                                                                                       cTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaa                                                          <  2:3906748/149‑1 (MQ=255)
                                                                                       cTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaa                                                          <  2:1866536/149‑1 (MQ=255)
                                                                                        tACAGAAAATAGTAAATAAAAAAAATAATACACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaat                                                         <  1:274315/149‑1 (MQ=255)
                                                                                        tACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaat                                                         <  1:1376355/149‑1 (MQ=255)
                                                                                        tACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaat                                                         >  1:2473955/1‑149 (MQ=255)
                                                                                        tACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaat                                                         <  1:951923/149‑1 (MQ=255)
                                                                                        tACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaat                                                         <  1:3307708/149‑1 (MQ=255)
                                                                                        tACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCAAGAGGATATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaat                                                         <  1:1064956/149‑1 (MQ=255)
                                                                                               aaTAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTTTCATTAATAGTTTTAATTCTGAAATCATGGGAGAAATGTCGAataataata                                                  >  1:2988964/1‑149 (MQ=255)
                                                                                               aaTAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAataataata                                                  >  2:3742383/1‑149 (MQ=255)
                                                                                                   gTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATaaa                                              >  2:2775340/1‑149 (MQ=255)
                                                                                                   gTAAATAAAAAAAACAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATaaa                                              >  2:2775230/1‑149 (MQ=255)
                                                                                                     aaataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAg                                            >  2:3337/1‑149 (MQ=255)
                                                                                                      aataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGt                                           <  1:956697/149‑1 (MQ=255)
                                                                                                      aataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGt                                           >  2:4092796/1‑149 (MQ=255)
                                                                                                      aataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGt                                           <  1:2501074/149‑1 (MQ=255)
                                                                                                      aataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGt                                           >  2:3952629/1‑149 (MQ=255)
                                                                                                       ataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAAATCATGGGAGAAATGTCGAATAATAATATAAAAGtt                                          >  2:102988/1‑149 (MQ=255)
                                                                                                          aaaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAt                                       <  1:3524058/149‑1 (MQ=255)
                                                                                                          aaaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAt                                       <  1:291412/149‑1 (MQ=255)
                                                                                                           aaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAtt                                      >  2:492343/1‑149 (MQ=255)
                                                                                                            aaaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAttt                                     >  2:1833660/1‑149 (MQ=255)
                                                                                                            aaaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAttt                                     >  2:863926/1‑149 (MQ=255)
                                                                                                             aaaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGt                                                              >  1:1105969/1‑123 (MQ=255)
                                                                                                              aaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTaa                                   >  1:140798/1‑149 (MQ=255)
                                                                                                               aataatAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTaaa                                  <  2:484519/149‑1 (MQ=255)
                                                                                                                   ataaACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATTaa                              >  2:3216887/1‑149 (MQ=255)
                                                                                                                   ataaACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGaa                              >  2:2066940/1‑149 (MQ=255)
                                                                                                                     aaaCTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAAcc                            <  2:2888278/149‑1 (MQ=255)
                                                                                                                       aCTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCag                          <  1:3055740/149‑1 (MQ=255)
                                                                                                                         tACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCagag                        >  1:2234153/1‑149 (MQ=255)
                                                                                                                          aCAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAgaga                       >  2:169516/1‑149 (MQ=255)
                                                                                                                           cAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGat                      <  1:807373/149‑1 (MQ=255)
                                                                                                                             aaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGataa                    >  1:3929969/1‑149 (MQ=255)
                                                                                                                             aaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGataa                    >  1:3369483/1‑149 (MQ=255)
                                                                                                                             aaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGataa                    >  1:1751777/1‑149 (MQ=255)
                                                                                                                               tAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGataata                  <  2:1460031/149‑1 (MQ=255)
                                                                                                                               tAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGataata                  <  2:3251843/149‑1 (MQ=255)
                                                                                                                                 aaaTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAtt                >  2:1554108/1‑149 (MQ=255)
                                                                                                                                   aTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAtttt              <  1:1446537/149‑1 (MQ=255)
                                                                                                                                   aTGCGTCATAGTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAtttt              <  1:3495483/149‑1 (MQ=255)
                                                                                                                                       gTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACa          <  2:2814079/149‑1 (MQ=255)
                                                                                                                                       gTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACa          <  2:2867200/149‑1 (MQ=255)
                                                                                                                                          atatTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGc       <  2:3562607/149‑1 (MQ=255)
                                                                                                                                          atatTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGc       >  1:2613940/1‑149 (MQ=255)
                                                                                                                                          atatTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGc       >  2:2244930/1‑149 (MQ=255)
                                                                                                                                          atatTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGc       >  1:549655/1‑149 (MQ=255)
                                                                                                                                           tatTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGcc      <  1:2393675/149‑1 (MQ=255)
                                                                                                                                           tatTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGcc      >  1:3784133/1‑149 (MQ=255)
                                                                                                                                           tatTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGcc      <  1:320740/149‑1 (MQ=255)
                                                                                                                                               cAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATc  >  2:2292065/1‑149 (MQ=255)
                                                                                                                                               cAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATc  >  2:216818/1‑149 (MQ=255)
                                                                                                                                               cAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATc  <  1:3571892/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGATC  >  CP009273/2376813‑2377104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑