Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,383,897 C→A 100% R363L (CGC→CTC)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,383,8970CA99.1% 728.8 / ‑1.7 222R363L (CGC→CTC) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (118/101);  total (120/102)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 222 total aligned reads displayed.

CCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGC  >  CP009273/2383751‑2384042
                                                                                                                                                  |                                                                                                                                                 
ccaccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAgg                                                                                                                                                 <  2:2080094/149‑1 (MQ=255)
ccaccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAgg                                                                                                                                                 >  2:3693442/1‑149 (MQ=255)
   ccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTaa                                                                                                                                              <  2:2756754/149‑1 (MQ=255)
      gCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAagag                                                                                                                                           >  2:1138898/1‑149 (MQ=255)
      gCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAagag                                                                                                                                           >  1:1251755/1‑149 (MQ=255)
       ccaccaCAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAgaga                                                                                                                                          <  1:3468693/149‑1 (MQ=255)
        caccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGaa                                                                                                                                         <  2:2206441/149‑1 (MQ=255)
        caccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGaa                                                                                                                                         >  1:2419082/1‑149 (MQ=255)
          ccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACa                                                                                                                                       <  1:602506/149‑1 (MQ=255)
          ccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACa                                                                                                                                       <  1:3502838/149‑1 (MQ=255)
             caAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAggg                                                                                                                                    <  2:3067620/149‑1 (MQ=255)
               aGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGaa                                                                                                                                  <  1:2978253/149‑1 (MQ=255)
                gtgtGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAAt                                                                                                                                 <  1:2359527/149‑1 (MQ=255)
                gtgtGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGAGACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAAt                                                                                                                                 <  2:528388/149‑1 (MQ=255)
                   tgCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAg                                                                                                                              >  1:1015882/1‑149 (MQ=255)
                       tGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATc                                                                                                                          >  1:530202/1‑149 (MQ=255)
                       tGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATc                                                                                                                          >  1:3098610/1‑149 (MQ=255)
                       tGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGCCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATc                                                                                                                          <  2:3638109/149‑1 (MQ=255)
                          acacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCgg                                                                                                                       >  1:251970/1‑149 (MQ=255)
                             ccgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGcc                                                                                                                    <  1:2445165/149‑1 (MQ=255)
                               gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCAc                                                                                                                  >  1:1083880/1‑149 (MQ=255)
                                   gCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTCAGAGAACAGGGAATCAGCATCCGGGCCACGATa                                                                                                              >  1:2819386/1‑149 (MQ=255)
                                   gCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATa                                                                                                              <  1:945975/149‑1 (MQ=255)
                                    ccAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATAc                                                                                                             >  1:3847447/1‑149 (MQ=255)
                                    ccAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATAc                                                                                                             >  1:2624270/1‑149 (MQ=255)
                                      aGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACgg                                                                                                           >  1:3416939/1‑149 (MQ=255)
                                       gCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACggg                                                                                                          >  2:2949885/1‑149 (MQ=255)
                                        cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGc                                                                                                         <  1:83347/149‑1 (MQ=255)
                                        cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGc                                                                                                         <  2:1421104/149‑1 (MQ=255)
                                            tAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGa                                                                                                     >  1:663346/1‑149 (MQ=255)
                                               aaCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAt                                                                                                  <  1:1940280/149‑1 (MQ=255)
                                               aaCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAt                                                                                                  >  1:6939/1‑149 (MQ=255)
                                                 cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCa                                                                                                <  2:1345602/149‑1 (MQ=255)
                                                 cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCa                                                                                                >  2:1287532/1‑149 (MQ=255)
                                                 cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCa                                                                                                <  2:1727311/149‑1 (MQ=255)
                                                   tACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAgg                                                                                              >  1:1390025/1‑149 (MQ=255)
                                                   tACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAgg                                                                                              >  2:2867831/1‑149 (MQ=255)
                                                    aCCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGc                                                                                             >  1:2262890/1‑149 (MQ=255)
                                                     ccGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCt                                                                                            <  2:767047/149‑1 (MQ=255)
                                                      cGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTg                                                                                           >  1:2752961/1‑149 (MQ=255)
                                                        aTAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGAc                                                                                         <  2:3613440/149‑1 (MQ=255)
                                                         tAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGAAACGGGCTGGACATCAGGCTGAcc                                                                                        <  1:2291541/149‑1 (MQ=255)
                                                          aaaTCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCa                                                                                       >  1:3758136/1‑149 (MQ=255)
                                                          aaaGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCa                                                                                       >  2:1178668/1‑149 (MQ=255)
                                                            aGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACg                                                                                     >  1:2800388/1‑149 (MQ=255)
                                                             gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGc                                                                                    >  2:69923/1‑149 (MQ=255)
                                                             gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGc                                                                                    >  2:2746426/1‑149 (MQ=255)
                                                                 aGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGaa                                                                                <  2:2733774/149‑1 (MQ=255)
                                                                 aGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGaa                                                                                >  2:1084725/1‑149 (MQ=255)
                                                                    gTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgc                                                                             <  2:530202/149‑1 (MQ=255)
                                                                       aTCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGcc                                                                          >  2:789439/1‑149 (MQ=255)
                                                                        tCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCg                                                                         >  2:2615587/1‑149 (MQ=255)
                                                                         cGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGa                                                                        <  2:3188694/149‑1 (MQ=255)
                                                                            gATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgc                                                                     <  2:1644979/149‑1 (MQ=255)
                                                                                ccGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctgct                                                                 <  1:765747/149‑1 (MQ=255)
                                                                                ccGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctgct                                                                 <  1:381/149‑1 (MQ=255)
                                                                                ccGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGgctgct                                                                 <  1:2606763/149‑1 (MQ=255)
                                                                                    ccAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAAc                                                             >  1:2066964/1‑149 (MQ=255)
                                                                                      agagACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                           >  2:2111033/1‑149 (MQ=255)
                                                                                        agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGAt                                                         <  2:580126/149‑1 (MQ=255)
                                                                                        agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGAt                                                         <  2:580212/149‑1 (MQ=255)
                                                                                        agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGAt                                                         <  2:3098610/149‑1 (MQ=255)
                                                                                           cAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAc                                                      >  2:2996994/1‑149 (MQ=255)
                                                                                            aGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAcc                                                     <  2:3831287/149‑1 (MQ=255)
                                                                                               atcatcACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGc                                                  <  1:597773/149‑1 (MQ=255)
                                                                                               atcatcACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGc                                                  >  2:2453429/1‑149 (MQ=255)
                                                                                                 catcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCa                                                >  2:1391939/1‑149 (MQ=255)
                                                                                                 catcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCa                                                >  1:1885628/1‑149 (MQ=255)
                                                                                                     aCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa                                            >  2:1542447/1‑149 (MQ=255)
                                                                                                       cGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTaaa                                          <  2:102941/149‑1 (MQ=255)
                                                                                                       cGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTaaa                                          <  2:1952650/149‑1 (MQ=255)
                                                                                                             caCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTa                                    >  1:3646757/1‑149 (MQ=255)
                                                                                                             caCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTa                                    >  2:3326459/1‑149 (MQ=255)
                                                                                                              agtGCCGCGAGGTTGGGACGATGCCTGAACAGACCGTGGTAAGTGAACAGGGAATCAGCATCCGGGCCACGATCCGGGCTGTACATCTGGCTGACCACGCCGAACGCGCCGTGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTAc                                   <  1:2867831/147‑1 (MQ=255)
                                                                                                                 gCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGc                                <  1:1421481/149‑1 (MQ=255)
                                                                                                                  ccGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCt                               <  2:613082/149‑1 (MQ=255)
                                                                                                                    gcgAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc                             >  2:3733196/1‑149 (MQ=255)
                                                                                                                      gAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCa                           >  2:3246930/1‑149 (MQ=255)
                                                                                                                       aGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCAt                          <  2:3867686/149‑1 (MQ=255)
                                                                                                                        ggATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATc                         >  1:3178906/1‑149 (MQ=255)
                                                                                                                        ggATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATc                         >  2:744665/1‑149 (MQ=255)
                                                                                                                        ggATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATc                         >  1:1872107/1‑149 (MQ=255)
                                                                                                                         gATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCg                        >  1:1256856/1‑149 (MQ=255)
                                                                                                                         gATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCg                        >  1:3398691/1‑149 (MQ=255)
                                                                                                                           tCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATAGGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGAc                      <  1:3949786/149‑1 (MQ=255)
                                                                                                                           tCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATACCCGGCCAGGTAAACCCCTACCGCTTCCATCGAc                      <  1:715634/149‑1 (MQ=255)
                                                                                                                            cGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACTGTCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACa                     >  1:4033947/1‑149 (MQ=255)
                                                                                                                             ggACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAt                    >  1:988009/1‑149 (MQ=255)
                                                                                                                             ggACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAt                    <  1:612201/149‑1 (MQ=255)
                                                                                                                              gACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                   >  1:2716901/1‑149 (MQ=255)
                                                                                                                              gACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                   >  2:2909957/1‑149 (MQ=255)
                                                                                                                              gACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                   <  2:2229288/149‑1 (MQ=255)
                                                                                                                              gACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                   <  2:668789/149‑1 (MQ=255)
                                                                                                                               aCGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCGGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtct                  <  2:736294/149‑1 (MQ=255)
                                                                                                                                   tGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGcc              >  2:3832068/1‑149 (MQ=255)
                                                                                                                                   tGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGcc              <  2:2325872/149‑1 (MQ=255)
                                                                                                                                      cAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGt           <  2:1885628/149‑1 (MQ=255)
                                                                                                                                       aGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGtt          <  1:1226497/149‑1 (MQ=255)
                                                                                                                                            aGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAg     <  1:3693442/149‑1 (MQ=255)
                                                                                                                                              aCCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgcg   >  2:3977955/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGC  >  CP009273/2383751‑2384042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑