BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,402,924	A→T	5.7%	Q195L (CAG→CTG)	yfbR →	5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,402,924	0	A	T	5.7%	773.1 / 25.7	248	Q195L (CAG→CTG)	yfbR	5'‑nucleotidase
Reads supporting (aligned to +/- strand): ref base A (103/130); new base T (5/10); total (108/140)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGTACCCCCAAC  >  CP009273/2402776‑2403071

cTGGAAGAACTCGCGGCCGGAAATAATTAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCa                                                                                                                                                     <  1:1237107/149‑1 (MQ=255)

 tGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAg                                                                                                                                                    <  1:2804573/149‑1 (MQ=255)

 tGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAg                                                                                                                                                    <  1:1978893/149‑1 (MQ=255)

  ggAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAgg                                                                                                                                                   >  2:3668351/1‑149 (MQ=255)

   gaagaaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGa                                                                                                                                                  <  2:3159308/149‑1 (MQ=255)

     agagCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGAGACGCTTGAACCGCGTAGCAGCAAGGAGATGGACTACTTCATGGAAATATTTGTACCCAGCTTCCATCTTTATCTCGATGAGATTAGCCAGGAtt                                                                                                                                                >  2:1456471/1‑149 (MQ=255)

     agaaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGAtt                                                                                                                                                >  2:2675585/1‑149 (MQ=255)

      gaaCTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTc                                                                                                                                               <  2:3024335/149‑1 (MQ=255)

                 cGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAag                                                                                                                                    <  2:2647419/149‑1 (MQ=255)

                   gAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAagca                                                                                                                                  >  2:2666730/1‑149 (MQ=255)

                   gAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAagca                                                                                                                                  <  2:1860879/149‑1 (MQ=255)

                   gAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAagca                                                                                                                                  <  2:1791252/149‑1 (MQ=255)

                        aatGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCgg                                                                                                                             >  1:2277129/1‑149 (MQ=255)

                        aatGAATTCGTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCgg                                                                                                                             <  2:1049332/149‑1 (MQ=255)

                          tGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAg                                                                                                                           >  2:2310929/1‑149 (MQ=255)

                           gAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGt                                                                                                                          >  2:245567/1‑149 (MQ=255)

                            aaTTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTc                                                                                                                         >  1:137886/1‑149 (MQ=255)

                            aaTTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTc                                                                                                                         >  1:2214936/1‑149 (MQ=255)

                            aaTTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTc                                                                                                                         <  2:1580417/149‑1 (MQ=255)

                             aTTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCt                                                                                                                        >  2:881900/1‑149 (MQ=255)

                              ttCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTg                                                                                                                       <  2:2413110/149‑1 (MQ=255)

                                 ttGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGt                                                                                                                    >  1:380052/1‑149 (MQ=255)

                                   gCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCg                                                                                                                  >  1:2672112/1‑149 (MQ=255)

                                   gCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCg                                                                                                                  >  1:2159937/1‑149 (MQ=255)

                                    cTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGGTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGc                                                                                                                 <  2:3155508/149‑1 (MQ=255)

                                     tGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCa                                                                                                                <  2:380052/149‑1 (MQ=255)

                                       gCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATc                                                                                                              <  2:325178/149‑1 (MQ=255)

                                          aaaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAgg                                                                                                           >  1:2565872/1‑149 (MQ=255)

                                          aaaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAgg                                                                                                           >  1:1419535/1‑149 (MQ=255)

                                           aaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGc                                                                                                          <  1:3542136/149‑1 (MQ=255)

                                           aaaCGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGGTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGc                                                                                                          <  1:410382/149‑1 (MQ=255)

                                                    tGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAgcgc                                                                                                 <  1:3431419/149‑1 (MQ=255)

                                                    tGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAgcgc                                                                                                 <  2:1829924/149‑1 (MQ=255)

                                                     ggAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGcc                                                                                                <  2:1971530/149‑1 (MQ=255)

                                                     ggAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGcc                                                                                                <  2:3741829/149‑1 (MQ=255)

                                                      gAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCg                                                                                               <  1:2393261/149‑1 (MQ=255)

                                                          cGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGTGTCTGCGTCGCATCAGGCAATAAGCGCCGGATg                                                                                           <  1:1438896/149‑1 (MQ=255)

                                                                ttGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACAt                                                                                     >  2:549821/1‑149 (MQ=255)

                                                                ttGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACAt                                                                                     >  2:701841/1‑149 (MQ=255)

                                                                ttGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACAt                                                                                     <  1:351783/149‑1 (MQ=255)

                                                                 tGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATc                                                                                    <  1:82259/149‑1 (MQ=255)

                                                                   aaGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAg                                                                                  >  1:3451629/1‑149 (MQ=255)

                                                                     gcgcGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGc                                                                                <  1:3740418/149‑1 (MQ=255)

                                                                     gcgcGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTGTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGc                                                                                <  1:3810964/149‑1 (MQ=255)

                                                                       gcgTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGctc                                                                              <  2:2794047/149‑1 (MQ=255)

                                                                           cGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGt                                                                          <  1:3138234/149‑1 (MQ=255)

                                                                              aGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCaa                                                                       >  2:23146/1‑149 (MQ=255)

                                                                                ccAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCaaaa                                                                     <  1:989681/149‑1 (MQ=255)

                                                                                ccAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCaaaa                                                                     <  2:2050498/149‑1 (MQ=255)

                                                                                   ggAGCTGGACGACGTCATGGAAATATGTGGTCCCAGCGTCCCTCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCGGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACgg                                                                  <  2:2292543/149‑1 (MQ=255)

                                                                                     agaTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGaa                                                                >  2:1911811/1‑149 (MQ=255)

                                                                                      gaTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGaaa                                                               <  2:4068047/149‑1 (MQ=255)

                                                                                       aTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAAc                                                              <  2:1689205/149‑1 (MQ=255)

                                                                                       aTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAAc                                                              <  2:3318992/149‑1 (MQ=255)

                                                                                       aTGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAAc                                                              <  2:3319190/149‑1 (MQ=255)

                                                                                         ggACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAg                                                            <  2:4045093/149‑1 (MQ=255)

                                                                                         ggACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAg                                                            <  2:2277129/149‑1 (MQ=255)

                                                                                         ggACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTATTGTCAAAACGGAAACAg                                                            >  2:1896038/1‑149 (MQ=255)

                                                                                          gACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGc                                                           <  2:3084498/149‑1 (MQ=255)

                                                                                           actactTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCa                                                          <  1:1813933/149‑1 (MQ=255)

                                                                                            ctactTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCAt                                                         <  2:3249356/149‑1 (MQ=255)

                                                                                             tactTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATc                                                        >  2:3589878/1‑149 (MQ=255)

                                                                                             tactTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATc                                                        >  1:690254/1‑149 (MQ=255)

                                                                                             tactTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATc                                                        >  1:1672077/1‑149 (MQ=255)

                                                                                             tactTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATc                                                        <  1:2401406/149‑1 (MQ=255)

                                                                                              actTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCg                                                       <  2:3594251/149‑1 (MQ=255)

                                                                                               ctTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCgg                                                      >  2:2147825/1‑149 (MQ=255)

                                                                                                ttCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCggg                                                     >  1:1193069/1‑149 (MQ=255)

                                                                                                 tcttGGAAATATTTGTTCCCAGCTTCCATATTTCGATCTATGAGATTATCCATGATTCACCGCTTTAAGAAGCCTGAGTCTGCTTCGCATCAGGCAATAATCGCAGTATGCGACATCAGGCTCTTTTCAAAACGGAAACAGATTCGGGa                                                    <  2:67079/146‑1 (MQ=255)

                                                                                                    tGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGatca                                                 <  1:3168035/149‑1 (MQ=255)

                                                                                                     ggAAATATTTGGTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCTGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGatcat                                                <  2:347656/149‑1 (MQ=255)

                                                                                                      gAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCATCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGatcatc                                               >  1:1254980/1‑149 (MQ=255)

                                                                                                        aaTATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATcac                                             >  1:398757/1‑149 (MQ=255)

                                                                                                           atTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATcaccac                                          <  2:1435221/149‑1 (MQ=255)

                                                                                                            tttGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACcaca                                         <  1:3860331/149‑1 (MQ=255)

                                                                                                             ttGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACcacac                                        <  2:1254328/149‑1 (MQ=255)

                                                                                                              tGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACcacaca                                       >  2:334951/1‑149 (MQ=255)

                                                                                                                ttCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACaaa                                     <  2:772918/149‑1 (MQ=255)

                                                                                                                  cccAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAAcc                                   >  2:2860203/1‑149 (MQ=255)

                                                                                                                      gCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCa                               >  2:2516021/1‑149 (MQ=255)

                                                                                                                      gCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCa                               >  2:1400721/1‑149 (MQ=255)

                                                                                                                      gCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCa                               <  2:2681456/149‑1 (MQ=255)

                                                                                                                       cTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCAt                              >  2:2452523/1‑149 (MQ=255)

                                                                                                                        ttCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATc                             >  1:2925226/1‑149 (MQ=255)

                                                                                                                        ttCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATc                             >  1:2171038/1‑149 (MQ=255)

                                                                                                                        ttCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTAAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATc                             <  1:2761922/149‑1 (MQ=255)

                                                                                                                         tCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCa                            >  2:3418777/1‑149 (MQ=255)

                                                                                                                               tttCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATaa                      >  1:1013982/1‑149 (MQ=255)

                                                                                                                               tttCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATaa                      >  2:3864845/1‑149 (MQ=255)

                                                                                                                               tttCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATaa                      <  1:1640392/149‑1 (MQ=255)

                                                                                                                                  cGCTCGATTAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATgg                   <  1:3668351/149‑1 (MQ=255)

                                                                                                                                      cGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGaa               >  2:476476/1‑149 (MQ=255)

                                                                                                                                       gATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAAc              <  2:3457061/149‑1 (MQ=255)

                                                                                                                                       gATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAAc              <  1:2982535/149‑1 (MQ=255)

                                                                                                                                       gATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAAc              >  2:1134034/1‑149 (MQ=255)

                                                                                                                                        aTGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACg             >  1:763874/1‑149 (MQ=255)

                                                                                                                                         tGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACgg            >  2:3153654/1‑149 (MQ=255)

                                                                                                                                          gagaTTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGt           >  1:507928/1‑149 (MQ=255)

                                                                                                                                             aTTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGTAcc        <  2:2565872/149‑1 (MQ=255)

                                                                                                                                                   cAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGTACCCCCAAc  <  1:1020173/149‑1 (MQ=255)

CTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCTTGAAGCGCGTCGCAGCCAGGAGATGGACTACTTCATGGAAATATTTGTTCCCAGCTTCCATCTTTCGCTCGATGAGATTAGCCAGGATTCACCGCTGTAAGCAGCCGGAGTCTGCGTCGCATCAGGCAATAAGCGCCGGATGCGACATCAGGCTCTTGTCAAAACGGAAACAGCATCGGGATCATCACCACACAAACCGCCATCACGATAATGGTGAACGGTACCCCCAAC  >  CP009273/2402776‑2403071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`