Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 25,546 G→T 100% D114Y (GAC→TAC)  lspA → prolipoprotein signal peptidase (signal peptidase II)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927325,5460GT100.0% 831.8 / ‑6.7 254D114Y (GAC→TAC) lspAprolipoprotein signal peptidase (signal peptidase II)
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (123/130);  total (123/131)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 254 total aligned reads displayed.

GCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCG  >  CP009273/25400‑25689
                                                                                                                                                  |                                                                                                                                               
gcggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTAc                                                                                                                                               <  2:1719828/149‑1 (MQ=255)
     tGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCt                                                                                                                                          <  2:201449/149‑1 (MQ=255)
     tGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCt                                                                                                                                          <  1:1648045/149‑1 (MQ=255)
        cAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtgtg                                                                                                                                       <  2:2147443/149‑1 (MQ=255)
         aGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTgg                                                                                                                                      >  2:2328054/1‑149 (MQ=255)
          gCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGc                                                                                                                                     >  2:4064774/1‑149 (MQ=255)
          gCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCCGGGCAACCTGTTCTACCGCCTGTGGc                                                                                                                                     >  2:1669762/1‑149 (MQ=255)
            gTTGGTTCTTTGCCGGAATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGAGGCGCGCAGCGCAACCTGATCTACCGCCTGTGGCAc                                                                                                                                   >  2:3817174/1‑149 (MQ=255)
              tGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACgg                                                                                                                                 >  2:506929/1‑149 (MQ=255)
              tGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACgg                                                                                                                                 >  1:3884814/1‑149 (MQ=255)
                 ttCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCtt                                                                                                                              <  2:2977524/149‑1 (MQ=255)
                    tttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGt                                                                                                                           <  1:1295808/149‑1 (MQ=255)
                     ttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGtt                                                                                                                          <  2:2581563/149‑1 (MQ=255)
                          ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGa                                                                                                                     >  2:3616595/1‑149 (MQ=255)
                            tATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGata                                                                                                                   <  2:1172178/149‑1 (MQ=255)
                             aTTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGatat                                                                                                                  <  1:2551037/149‑1 (MQ=255)
                             aTTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGatat                                                                                                                  >  1:3325856/1‑149 (MQ=255)
                              ttGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATg                                                                                                                 >  1:1715863/1‑149 (MQ=255)
                               tGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGa                                                                                                                <  1:1080789/149‑1 (MQ=255)
                                 cGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATc                                                                                                              <  1:1534026/149‑1 (MQ=255)
                                 cGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATc                                                                                                              <  1:1214097/149‑1 (MQ=255)
                                   aTTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGa                                                                                                            >  2:974648/1‑149 (MQ=255)
                                     tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACt                                                                                                          <  2:2198931/149‑1 (MQ=255)
                                     tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACt                                                                                                          <  1:2135413/149‑1 (MQ=255)
                                      ggTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACtt                                                                                                         >  1:1769969/1‑149 (MQ=255)
                                       gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                        <  2:3958343/149‑1 (MQ=255)
                                       gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                        >  1:123230/1‑149 (MQ=255)
                                       gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                        >  2:2468432/1‑149 (MQ=255)
                                       gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                        <  2:1799299/149‑1 (MQ=255)
                                         aTTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTa                                                                                                      >  1:2634254/1‑149 (MQ=255)
                                         aTTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTa                                                                                                      <  2:1609387/149‑1 (MQ=255)
                                          ttAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTAc                                                                                                     >  2:3118531/1‑149 (MQ=255)
                                               gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCgg                                                                                                >  1:143920/1‑149 (MQ=255)
                                                  aTCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGa                                                                                             >  1:3597368/1‑149 (MQ=255)
                                                   tCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGAc                                                                                            <  2:853853/149‑1 (MQ=255)
                                                    ccTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACt                                                                                           >  2:3523549/1‑149 (MQ=255)
                                                       ggCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGc                                                                                        <  1:2451383/149‑1 (MQ=255)
                                                       ggCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGc                                                                                        >  1:1035869/1‑149 (MQ=255)
                                                           gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACtt                                                                                    >  1:3717520/1‑149 (MQ=255)
                                                           gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACtt                                                                                    >  1:3976647/1‑149 (MQ=255)
                                                           gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACtt                                                                                    >  2:3906275/1‑149 (MQ=255)
                                                            tgatgaTGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTc                                                                                   >  1:300109/1‑149 (MQ=255)
                                                             gatgatGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCg                                                                                  >  2:1096690/1‑149 (MQ=255)
                                                                  tgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCAcc                                                                             >  1:2414063/1‑149 (MQ=255)
                                                                          tCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCt                                                                     >  1:2943799/1‑149 (MQ=255)
                                                                          tCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCt                                                                     <  2:3199642/149‑1 (MQ=255)
                                                                           cGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCtt                                                                    >  2:1413010/1‑149 (MQ=255)
                                                                           cGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCtt                                                                    >  2:3459811/1‑149 (MQ=255)
                                                                             aaGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGc                                                                  <  1:3907610/149‑1 (MQ=255)
                                                                               ggCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCg                                                                >  1:1108260/1‑149 (MQ=255)
                                                                                gCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGa                                                               <  1:3394849/149‑1 (MQ=255)
                                                                                   aCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATAc                                                            <  2:2523043/149‑1 (MQ=255)
                                                                                      cAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGc                                                         >  1:664137/1‑149 (MQ=255)
                                                                                      cAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGc                                                         >  1:3796651/1‑149 (MQ=255)
                                                                                       aGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGcc                                                        >  1:1597679/1‑149 (MQ=255)
                                                                                        gAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCa                                                       <  2:2381778/149‑1 (MQ=255)
                                                                                         aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCAt                                                      <  1:2685733/149‑1 (MQ=255)
                                                                                         aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCAt                                                      <  1:483917/149‑1 (MQ=255)
                                                                                         aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCAt                                                      <  1:3528125/149‑1 (MQ=255)
                                                                                            cTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtg                                                   <  1:2093329/149‑1 (MQ=255)
                                                                                             tAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgt                                                  <  1:223325/149‑1 (MQ=255)
                                                                                              aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                 <  2:3896798/149‑1 (MQ=255)
                                                                                              aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                 <  2:2832082/149‑1 (MQ=255)
                                                                                               aaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                <  2:1497466/149‑1 (MQ=255)
                                                                                               aaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                <  2:1102616/149‑1 (MQ=255)
                                                                                                 cAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCg                                              <  2:3253026/149‑1 (MQ=255)
                                                                                                  aaTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCgg                                             >  1:2743225/1‑149 (MQ=255)
                                                                                                  aaTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCgg                                             <  2:2702359/149‑1 (MQ=255)
                                                                                                     atCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGc                                          >  1:3660336/1‑149 (MQ=255)
                                                                                                         cTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCa                                      >  2:3092193/1‑149 (MQ=255)
                                                                                                          ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCAc                                     <  2:1277375/149‑1 (MQ=255)
                                                                                                                 cTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgt                              <  1:2344287/149‑1 (MQ=255)
                                                                                                                 cTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgt                              <  1:1514770/149‑1 (MQ=255)
                                                                                                                  tGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                             >  1:2802511/1‑149 (MQ=255)
                                                                                                                   gATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACATTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGc                            <  1:2118358/149‑1 (MQ=255)
                                                                                                                    attattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCt                           <  2:3022905/149‑1 (MQ=255)
                                                                                                                     ttattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                          <  1:886301/149‑1 (MQ=255)
                                                                                                                     ttattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                          >  1:1940241/1‑149 (MQ=255)
                                                                                                                     ttattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                          <  1:2206682/149‑1 (MQ=255)
                                                                                                                       attGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGGGCGGCACTGATTGTGCTGGa                        >  2:3407999/1‑149 (MQ=255)
                                                                                                                           gcggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGt                    <  2:2235648/149‑1 (MQ=255)
                                                                                                                           gcggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGt                    <  1:311754/149‑1 (MQ=255)
                                                                                                                           gcggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAGTGTGCTGGAAGGt                    >  2:3289986/1‑149 (MQ=255)
                                                                                                                              gcgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                 >  1:1902850/1‑149 (MQ=255)
                                                                                                                              gcgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                 >  2:1379775/1‑149 (MQ=255)
                                                                                                                               cgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttttt                <  1:3196813/149‑1 (MQ=255)
                                                                                                                                gcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttttt               <  2:4105895/149‑1 (MQ=255)
                                                                                                                                    tGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCt           >  2:1399286/1‑149 (MQ=255)
                                                                                                                                     gggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACATTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCtt          >  2:3986499/1‑149 (MQ=255)
                                                                                                                                     gggCAACCTGTTCTACCGCCAGTGGCACGGCATCGTTGTCGAAATGAACCACTTAAACGTAGGCGACTGGCACTTCGCCACCTACAACCTTACCCATACTGACATCAGTGTCGCTGAGGCACTGATTGTGCTGGAAGGTTTTTTGACtt          >  2:569418/1‑149 (MQ=255)
                                                                                                                                       gCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCt        <  2:3681343/149‑1 (MQ=255)
                                                                                                                                       gCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCt        <  2:1715854/149‑1 (MQ=255)
                                                                                                                                       gCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCt        <  1:2504186/149‑1 (MQ=255)
                                                                                                                                           ccTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTagag    >  1:3477178/1‑149 (MQ=255)
                                                                                                                                           ccTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTagag    >  2:2274184/1‑149 (MQ=255)
                                                                                                                                            cTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGc   <  2:3756277/149‑1 (MQ=255)
                                                                                                                                            cTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGc   >  2:809245/1‑149 (MQ=255)
                                                                                                                                             tGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCg  <  1:436063/149‑1 (MQ=255)
                                                                                                                                             tGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCg  <  1:99224/149‑1 (MQ=255)
                                                                                                                                             tGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCg  >  1:1090444/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCG  >  CP009273/25400‑25689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑