Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,476,524 T→A 100% N99Y (AAT→TAT)  emrK ← EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,476,5240TA98.2% 726.2 / 1.8 225N99Y (AAT→TAT) emrKEmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base A (102/119);  total (102/123)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

AACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTG  >  CP009273/2476378‑2476669
                                                                                                                                                  |                                                                                                                                                 
 aCCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTAtattt                                                                                                                                                <  2:1835382/149‑1 (MQ=255)
 aCCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTAtattt                                                                                                                                                <  2:532358/149‑1 (MQ=255)
   ccccTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAg                                                                                                                                              >  1:2800398/1‑149 (MQ=255)
   ccccTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAg                                                                                                                                              <  1:3489193/149‑1 (MQ=255)
   ccccTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAg                                                                                                                                              <  2:2682561/149‑1 (MQ=255)
      cTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCtt                                                                                                                                           >  1:3548152/1‑149 (MQ=255)
        gCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTa                                                                                                                                         <  2:2624309/149‑1 (MQ=255)
        gCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTa                                                                                                                                         >  1:3767549/1‑149 (MQ=255)
        gCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTa                                                                                                                                         <  1:3894235/149‑1 (MQ=255)
         cTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTAt                                                                                                                                        <  1:552369/149‑1 (MQ=255)
         cTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTAt                                                                                                                                        <  1:228455/149‑1 (MQ=255)
         cTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTAt                                                                                                                                        <  2:3494487/149‑1 (MQ=255)
          ttCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTAtt                                                                                                                                       <  1:3238612/149‑1 (MQ=255)
              cTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGt                                                                                                                                   >  1:1119973/1‑149 (MQ=255)
                  cGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGa                                                                                                                               <  2:1746500/149‑1 (MQ=255)
                  cGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGa                                                                                                                               <  1:2953293/149‑1 (MQ=255)
                   ggCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGAt                                                                                                                              <  1:3641787/149‑1 (MQ=255)
                      aCTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGt                                                                                                                           <  1:1405635/149‑1 (MQ=255)
                         cGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGc                                                                                                                        >  1:398844/1‑149 (MQ=255)
                             ggTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCa                                                                                                                    <  2:505332/149‑1 (MQ=255)
                             ggTTATAATCTTCTAAAGATTGTTGATACTGAAGACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCa                                                                                                                    <  2:1419665/149‑1 (MQ=255)
                                tataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGtt                                                                                                                 <  1:4044231/149‑1 (MQ=255)
                                tataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGtt                                                                                                                 <  1:3952574/149‑1 (MQ=255)
                                tataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGtt                                                                                                                 <  1:1914836/149‑1 (MQ=255)
                                 ataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGttt                                                                                                                >  2:2429659/1‑149 (MQ=255)
                                  taATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGtttt                                                                                                               >  2:2151862/1‑149 (MQ=255)
                                   aaTCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTa                                                                                                              <  1:2637091/149‑1 (MQ=255)
                                   aaTCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTAGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTa                                                                                                              <  2:1108587/149‑1 (MQ=255)
                                   aaTCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTAGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTa                                                                                                              <  2:3160594/149‑1 (MQ=255)
                                     tcttctAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCATGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATc                                                                                                            <  1:3532646/149‑1 (MQ=255)
                                        tctAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAg                                                                                                         <  2:3181400/149‑1 (MQ=255)
                                          tAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTAGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGTACAATATTTGCCAGATTATATTTAGCTTTATTGAGTTCGATAGTGGGATCAGTTTTTTCCAGTg                                                                                                       <  2:433016/149‑1 (MQ=255)
                                           aaaGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGa                                                                                                      >  1:1150488/1‑149 (MQ=255)
                                             aGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGaaa                                                                                                    >  1:3797324/1‑149 (MQ=255)
                                               aTTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACt                                                                                                  <  1:3720987/149‑1 (MQ=255)
                                               aTTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTGTTATCCAGTGAAACt                                                                                                  >  2:2548061/1‑149 (MQ=255)
                                                   ttgATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTaaaa                                                                                              >  1:487918/1‑149 (MQ=255)
                                                   ttgATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTaaaa                                                                                              <  1:154541/149‑1 (MQ=255)
                                                    tgATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAAt                                                                                             >  1:879706/1‑149 (MQ=255)
                                                         cTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCAc                                                                                        <  1:3449933/149‑1 (MQ=255)
                                                         cTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCAc                                                                                        <  1:1030423/149‑1 (MQ=255)
                                                         cTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCAc                                                                                        <  1:3327938/149‑1 (MQ=255)
                                                          tGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCAcc                                                                                       <  2:1702327/149‑1 (MQ=255)
                                                           gAATACGTGCTGAAGCGACTTCGGCACTGTATTGTCTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCt                                                                                      <  2:1368649/149‑1 (MQ=255)
                                                            aaTACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCtt                                                                                     >  1:2171554/1‑149 (MQ=255)
                                                            aaTACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCtt                                                                                     <  2:2240920/149‑1 (MQ=255)
                                                              tACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGACGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGt                                                                                   <  1:31029/149‑1 (MQ=255)
                                                                cGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCg                                                                                 >  2:1573546/1‑149 (MQ=255)
                                                                  tGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGaa                                                                               <  2:3331121/149‑1 (MQ=255)
                                                                  tGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGaa                                                                               <  2:1346213/149‑1 (MQ=255)
                                                                   gCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAAc                                                                              <  1:1093297/149‑1 (MQ=255)
                                                                        aGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGt                                                                         <  1:986473/149‑1 (MQ=255)
                                                                         gCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGtt                                                                        <  1:3567628/149‑1 (MQ=255)
                                                                         gCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGtt                                                                        <  2:1476266/149‑1 (MQ=255)
                                                                           gACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCg                                                                      <  1:3758643/149‑1 (MQ=255)
                                                                            aCTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGt                                                                     >  1:1970354/1‑149 (MQ=255)
                                                                            aCTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGt                                                                     >  1:2057442/1‑149 (MQ=255)
                                                                             cTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTa                                                                    <  2:2467677/149‑1 (MQ=255)
                                                                             cTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTa                                                                    <  2:4059945/149‑1 (MQ=255)
                                                                             cTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTa                                                                    <  1:1881811/149‑1 (MQ=255)
                                                                                 ggCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTTAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCtt                                                                >  1:1474119/1‑149 (MQ=255)
                                                                                 ggCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTGTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCtt                                                                <  2:1782129/149‑1 (MQ=255)
                                                                                  gCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCttt                                                               >  2:3034646/1‑149 (MQ=255)
                                                                                    aCTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTAt                                                             >  2:2091930/1‑149 (MQ=255)
                                                                                    aCTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTAt                                                             >  1:2426005/1‑149 (MQ=255)
                                                                                    aCTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTAt                                                             <  2:3756686/149‑1 (MQ=255)
                                                                                      tGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACATTGTCGAACTTAGTTCGTATCTTTATGa                                                           >  1:1322856/1‑149 (MQ=255)
                                                                                       gTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGAt                                                          >  2:30157/1‑149 (MQ=255)
                                                                                           tGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAAc                                                      <  2:3346249/149‑1 (MQ=255)
                                                                                            gTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACg                                                     >  2:887481/1‑149 (MQ=255)
                                                                                            gTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACg                                                     >  2:885348/1‑149 (MQ=255)
                                                                                            gTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACg                                                     >  1:3347268/1‑149 (MQ=255)
                                                                                             tttATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGa                                                    <  1:3691126/149‑1 (MQ=255)
                                                                                              ttATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGAc                                                   <  2:461678/149‑1 (MQ=255)
                                                                                              ttATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGAc                                                   <  1:475852/149‑1 (MQ=255)
                                                                                               tATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACa                                                  <  1:1798744/149‑1 (MQ=255)
                                                                                                 tCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                >  2:3843114/1‑149 (MQ=255)
                                                                                                 tCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                >  2:3841802/1‑149 (MQ=255)
                                                                                                 tCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                >  2:3590721/1‑149 (MQ=255)
                                                                                                       aaGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACt                                          >  2:4075324/1‑149 (MQ=255)
                                                                                                               tttATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTgaga                                  >  2:2027777/1‑149 (MQ=255)
                                                                                                               tttATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTgaga                                  >  1:747178/1‑149 (MQ=255)
                                                                                                                 tATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACt                                >  1:1712598/1‑149 (MQ=255)
                                                                                                                   ttCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATATTTATGATTAACGACAGTGACACTACCTGAGACTtg                              <  1:3112625/149‑1 (MQ=255)
                                                                                                                       tttGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCa                          >  1:2547445/1‑149 (MQ=255)
                                                                                                                       tttGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCa                          >  2:2925015/1‑149 (MQ=255)
                                                                                                                         tGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGa                        >  2:358516/1‑149 (MQ=255)
                                                                                                                         tGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGa                        >  2:1957613/1‑149 (MQ=255)
                                                                                                                            cGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAt                     >  1:1680497/1‑149 (MQ=255)
                                                                                                                             gAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAtt                    >  1:1761555/1‑149 (MQ=255)
                                                                                                                              aaCAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTg                   >  2:3410763/1‑149 (MQ=255)
                                                                                                                               aCAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTgg                  <  1:683290/149‑1 (MQ=255)
                                                                                                                               aCAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTgg                  >  2:1195128/1‑149 (MQ=255)
                                                                                                                                 aaTATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGAt                <  2:3198944/149‑1 (MQ=255)
                                                                                                                                     tgtGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTCTCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGc            <  1:2364656/147‑1 (MQ=255)
                                                                                                                                       tGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAt          <  1:2377466/149‑1 (MQ=255)
                                                                                                                                            gATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTcccc     <  1:1527337/149‑1 (MQ=255)
                                                                                                                                            gATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTcacc     >  2:2407573/1‑149 (MQ=255)
                                                                                                                                             attataTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCg    >  2:83516/1‑149 (MQ=255)
                                                                                                                                               tataTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTg  >  1:3493572/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTG  >  CP009273/2476378‑2476669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑