Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,481,974 G→T 100% S225* (TCA→TAA)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,481,9740GT99.6% 794.6 / ‑4.4 238S225* (TCA→TAA) yfdEacetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (131/106);  total (132/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

ATTAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCC  >  CP009273/2481829‑2482122
                                                                                                                                                 |                                                                                                                                                    
aTTAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACt                                                                                                                                                   <  2:3968233/149‑1 (MQ=255)
 ttAACCAGTTCCTTAAGCTCCAGTGCATGGAATAACTCATAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACtt                                                                                                                                                  <  2:1202524/149‑1 (MQ=255)
 ttAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACtt                                                                                                                                                  >  1:4102885/1‑149 (MQ=255)
  tAACCAGGTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTc                                                                                                                                                 <  2:117986/149‑1 (MQ=255)
   aaCCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTcc                                                                                                                                                <  2:1099638/149‑1 (MQ=255)
    aCCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTccc                                                                                                                                               >  1:1047976/1‑149 (MQ=255)
    aCCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTccc                                                                                                                                               >  1:3565999/1‑149 (MQ=255)
    aCCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTccc                                                                                                                                               >  1:386373/1‑149 (MQ=255)
     ccAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCa                                                                                                                                              <  2:2147507/149‑1 (MQ=255)
     ccAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCa                                                                                                                                              <  1:3409642/149‑1 (MQ=255)
     ccAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCa                                                                                                                                              <  1:3408498/149‑1 (MQ=255)
       aGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGt                                                                                                                                            >  2:2176592/1‑149 (MQ=255)
       aGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGt                                                                                                                                            <  1:1272222/149‑1 (MQ=255)
         ttCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTg                                                                                                                                          >  2:3619232/1‑149 (MQ=255)
             gTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGat                                                                                                                                      <  1:2662000/149‑1 (MQ=255)
              tAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGata                                                                                                                                     >  1:70509/1‑149 (MQ=255)
              tAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGata                                                                                                                                     >  1:3582805/1‑149 (MQ=255)
              tAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGata                                                                                                                                     <  1:3156916/149‑1 (MQ=255)
              tAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGata                                                                                                                                     >  2:3749360/1‑149 (MQ=255)
                       gTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATc                                                                                                                            >  2:753583/1‑149 (MQ=255)
                           cTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGAc                                                                                                                        <  1:981036/149‑1 (MQ=255)
                            tGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGAcc                                                                                                                       >  2:3685915/1‑149 (MQ=255)
                             ggCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCa                                                                                                                      <  1:131041/149‑1 (MQ=255)
                             ggCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCa                                                                                                                      >  1:1866378/1‑149 (MQ=255)
                                aTAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGc                                                                                                                   <  1:293858/149‑1 (MQ=255)
                                    cGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCa                                                                                                               <  1:2468022/149‑1 (MQ=255)
                                       aGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGaa                                                                                                            <  1:331208/149‑1 (MQ=255)
                                       aGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGaa                                                                                                            >  2:4007914/1‑149 (MQ=255)
                                        gAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGaaa                                                                                                           >  2:1722749/1‑149 (MQ=255)
                                         aaaaaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGaaaa                                                                                                          >  1:839336/1‑149 (MQ=255)
                                          aaaaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAAc                                                                                                         >  2:804878/1‑149 (MQ=255)
                                              aGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAg                                                                                                     >  2:1621298/1‑149 (MQ=255)
                                               gCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGc                                                                                                    <  1:1976917/149‑1 (MQ=255)
                                                 ttGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGt                                                                                                  <  2:536554/149‑1 (MQ=255)
                                                 ttGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGt                                                                                                  <  2:2580046/149‑1 (MQ=255)
                                                     cATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCa                                                                                              <  2:2885817/149‑1 (MQ=255)
                                                      aTTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCAt                                                                                             >  2:1064275/1‑149 (MQ=255)
                                                       ttACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATc                                                                                            <  1:491277/149‑1 (MQ=255)
                                                        tACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCa                                                                                           >  1:4036912/1‑149 (MQ=255)
                                                        tACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCa                                                                                           >  1:3005292/1‑149 (MQ=255)
                                                         aCCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCaa                                                                                          <  1:1440879/149‑1 (MQ=255)
                                                             caacaaATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACAt                                                                                      >  1:663731/1‑149 (MQ=255)
                                                              aacaaATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATc                                                                                     <  1:3623947/149‑1 (MQ=255)
                                                                caaATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGc                                                                                   <  2:3148756/149‑1 (MQ=255)
                                                                 aaaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGACAACTCAGCGTGGCATCAAACATCGCt                                                                                  >  2:2687437/1‑149 (MQ=255)
                                                                 aaaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCt                                                                                  >  2:622943/1‑149 (MQ=255)
                                                                 aaaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCt                                                                                  >  1:2249393/1‑149 (MQ=255)
                                                                 aaaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCt                                                                                  >  2:3122279/1‑149 (MQ=255)
                                                                  aaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCta                                                                                 <  1:1082066/149‑1 (MQ=255)
                                                                  aaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCta                                                                                 >  2:3063762/1‑149 (MQ=255)
                                                                  aaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCta                                                                                 >  1:19477/1‑149 (MQ=255)
                                                                   aTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCtat                                                                                <  1:3958470/149‑1 (MQ=255)
                                                                    tCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCtata                                                                               >  1:2809056/1‑149 (MQ=255)
                                                                    tCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCtata                                                                               >  1:1505791/1‑149 (MQ=255)
                                                                        aaTCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGa                                                                           <  2:3509735/149‑1 (MQ=255)
                                                                        aaTCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGa                                                                           <  2:3711976/149‑1 (MQ=255)
                                                                          tCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACa                                                                         >  2:2198587/1‑149 (MQ=255)
                                                                           cGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACAt                                                                        <  1:2127042/149‑1 (MQ=255)
                                                                             gCTTATCCTGAGTATTGAAAACATCTAATGTTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTTGTTACTTCCCAGTTGCTATATATGCCATCAGACAATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATgc                                                                      <  2:46884/149‑1 (MQ=255)
                                                                             gCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATgc                                                                      <  1:713/149‑1 (MQ=255)
                                                                               ttATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATgcgc                                                                    >  2:899951/1‑149 (MQ=255)
                                                                               ttATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATgcgc                                                                    >  2:3638723/1‑149 (MQ=255)
                                                                                  tCCTGAGTAATGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGcccc                                                                 <  2:242661/149‑1 (MQ=255)
                                                                                    cTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCctc                                                               >  1:547014/1‑149 (MQ=255)
                                                                                     tGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCctct                                                              <  2:3565999/149‑1 (MQ=255)
                                                                                     tGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCctct                                                              >  2:1600951/1‑149 (MQ=255)
                                                                                     tGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCctct                                                              <  1:91034/149‑1 (MQ=255)
                                                                                            tGAAAACATCAACAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGctc                                                       <  2:3639095/149‑1 (MQ=255)
                                                                                             gAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGctct                                                      >  2:1825848/1‑149 (MQ=255)
                                                                                             gAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGctct                                                      >  1:1458135/1‑149 (MQ=255)
                                                                                               ataCATCAAAATGTGCCATGTAGGTATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGTGATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCttt                                                    <  1:2736407/147‑1 (MQ=255)
                                                                                                 aCATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTc                                                  >  2:4037068/1‑149 (MQ=255)
                                                                                                   aTCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTcgc                                                >  1:2611474/1‑149 (MQ=255)
                                                                                                   aTCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTcgc                                                >  2:2025914/1‑149 (MQ=255)
                                                                                                      aaaaGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTCGCTCTTTTCGCGGc                                             <  2:1957633/149‑1 (MQ=255)
                                                                                                       aaaGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGcc                                            >  2:255603/1‑149 (MQ=255)
                                                                                                        aaGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCa                                           >  2:685357/1‑149 (MQ=255)
                                                                                                           gTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATaa                                        <  1:2254763/149‑1 (MQ=255)
                                                                                                              ccATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGt                                     >  2:3514687/1‑149 (MQ=255)
                                                                                                                 tGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGca                                  >  1:603883/1‑149 (MQ=255)
                                                                                                                  gTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcac                                 >  2:2431558/1‑149 (MQ=255)
                                                                                                                  gTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcac                                 <  2:2343095/149‑1 (MQ=255)
                                                                                                                    aGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactc                               >  2:3928747/1‑149 (MQ=255)
                                                                                                                     gggATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactca                              <  2:702953/149‑1 (MQ=255)
                                                                                                                     gggATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactca                              >  1:2559163/1‑149 (MQ=255)
                                                                                                                     gggATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactca                              <  2:2995960/149‑1 (MQ=255)
                                                                                                                       gATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactcact                            <  1:3853408/149‑1 (MQ=255)
                                                                                                                        aTGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGTCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTa                           <  2:1021607/149‑1 (MQ=255)
                                                                                                                        aTGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTa                           <  1:686011/149‑1 (MQ=255)
                                                                                                                         tGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTAt                          <  2:1798560/149‑1 (MQ=255)
                                                                                                                           gCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTc                        <  2:580833/149‑1 (MQ=255)
                                                                                                                               tttCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCt                    >  2:2642730/1‑149 (MQ=255)
                                                                                                                                  cccAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGaa                 <  1:1639769/149‑1 (MQ=255)
                                                                                                                                     aGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGaataa              >  1:42640/1‑149 (MQ=255)
                                                                                                                                     aGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGaataa              <  1:1270700/149‑1 (MQ=255)
                                                                                                                                      gACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGaataaa             >  2:989945/1‑149 (MQ=255)
                                                                                                                                         gTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGACTAAATAg          >  2:3319631/1‑149 (MQ=255)
                                                                                                                                           tgtgGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGac        <  1:2591325/149‑1 (MQ=255)
                                                                                                                                           tgtgGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGac        >  1:3012814/1‑149 (MQ=255)
                                                                                                                                            gtggttACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGaca       >  2:1309975/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATTAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCC  >  CP009273/2481829‑2482122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑