BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,531,188	C→A	6.6%	V27V (GTC→GTA)	yfeS →	WGR domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,531,188	0	C	A	6.6%	825.6 / 20.2	229	V27V (GTC→GTA)	yfeS	WGR domain protein
Reads supporting (aligned to +/- strand): ref base C (115/98); new base A (7/9); total (122/107)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

AGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCT  >  CP009273/2531041‑2531336

 gACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCaa                                                                                                                                                    >  1:1080205/1‑149 (MQ=255)

 gACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCaa                                                                                                                                                    >  1:3630533/1‑149 (MQ=255)

  aCGGATAAACCGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAAc                                                                                                                                                   <  2:69031/149‑1 (MQ=255)

     gATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTAt                                                                                                                                                >  2:3276253/1‑149 (MQ=255)

      aTAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATg                                                                                                                                               >  1:4113501/1‑149 (MQ=255)

         aaaCGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCa                                                                                                                                            >  2:2192389/1‑149 (MQ=255)

           aCGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGTTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAag                                                                                                                                          <  1:3095203/149‑1 (MQ=255)

           aCGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAag                                                                                                                                          <  1:1181623/149‑1 (MQ=255)

           aCGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAag                                                                                                                                          <  2:223269/149‑1 (MQ=255)

           aCGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAag                                                                                                                                          <  2:2347559/149‑1 (MQ=255)

           aCGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAag                                                                                                                                          <  1:3768111/149‑1 (MQ=255)

            cGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAagg                                                                                                                                         >  2:3656508/1‑149 (MQ=255)

            cGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAagg                                                                                                                                         <  1:1505284/149‑1 (MQ=255)

             ggTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggt                                                                                                                                        <  2:915116/149‑1 (MQ=255)

             ggTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggt                                                                                                                                        <  1:4105064/149‑1 (MQ=255)

              gTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggta                                                                                                                                       <  2:1856431/149‑1 (MQ=255)

               tGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtag                                                                                                                                      <  1:280286/149‑1 (MQ=255)

                    gCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGgtagt                                                                                                                                 >  1:2091110/1‑149 (MQ=255)

                     cGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGgtagta                                                                                                                                <  2:2289846/149‑1 (MQ=255)

                     cGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGgtagta                                                                                                                                >  2:329013/1‑149 (MQ=255)

                      ggaaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTAt                                                                                                                               >  2:2379501/1‑149 (MQ=255)

                       gaaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTAtt                                                                                                                              >  1:3236843/1‑149 (MQ=255)

                       gaaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTAtt                                                                                                                              <  2:3437292/149‑1 (MQ=255)

                       gaaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTAtt                                                                                                                              <  1:3927305/149‑1 (MQ=255)

                        aaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTCTTg                                                                                                                             >  2:2515829/1‑149 (MQ=255)

                        aaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTg                                                                                                                             >  2:145224/1‑149 (MQ=255)

                        aaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTg                                                                                                                             <  1:3504674/149‑1 (MQ=255)

                        aaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTg                                                                                                                             >  2:1881409/1‑149 (MQ=255)

                        aaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTg                                                                                                                             >  2:3922586/1‑149 (MQ=255)

                            aaaTTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTaa                                                                                                                         <  2:713749/149‑1 (MQ=255)

                               ttAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAAtt                                                                                                                      <  1:2026138/149‑1 (MQ=255)

                               ttAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAAtt                                                                                                                      <  1:4061935/149‑1 (MQ=255)

                               ttAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAAtt                                                                                                                      <  1:575680/149‑1 (MQ=255)

                                 aaTCCGCTTTGGGAAGGCATTTACAGGATGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTcc                                                                                                                    <  1:3158854/149‑1 (MQ=255)

                                    ccGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGa                                                                                                                 >  1:3827873/1‑149 (MQ=255)

                                      gCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACa                                                                                                               >  2:224533/1‑149 (MQ=255)

                                           gggAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAaga                                                                                                          <  2:880165/149‑1 (MQ=255)

                                           gggAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAaga                                                                                                          <  2:2939368/149‑1 (MQ=255)

                                             gAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGt                                                                                                        <  1:535794/149‑1 (MQ=255)

                                             gAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGt                                                                                                        <  1:596401/149‑1 (MQ=255)

                                             gAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGt                                                                                                        <  2:1541649/149‑1 (MQ=255)

                                              aaGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGtt                                                                                                       <  2:3943359/149‑1 (MQ=255)

                                                  cATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGAt                                                                                                   >  1:3605000/1‑149 (MQ=255)

                                                    tttACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATaa                                                                                                 >  2:1957501/1‑149 (MQ=255)

                                                    tttACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATaa                                                                                                 >  1:3005805/1‑149 (MQ=255)

                                                    tttACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATaa                                                                                                 >  2:1656071/1‑149 (MQ=255)

                                                          ggaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaaga                                                                                           <  2:3493872/149‑1 (MQ=255)

                                                          ggaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaaga                                                                                           >  2:3397138/1‑149 (MQ=255)

                                                            aggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAAc                                                                                         >  2:2017760/1‑149 (MQ=255)

                                                              gTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAg                                                                                       <  1:3837214/149‑1 (MQ=255)

                                                              gTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAg                                                                                       <  1:3326073/149‑1 (MQ=255)

                                                              gTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAg                                                                                       >  2:3737248/1‑149 (MQ=255)

                                                                 aCATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAgtgt                                                                                    >  2:380967/1‑149 (MQ=255)

                                                                 aCATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAgtgt                                                                                    >  1:1736158/1‑149 (MQ=255)

                                                                   aTGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCt                                                                                  >  2:36064/1‑149 (MQ=255)

                                                                    tGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTg                                                                                 <  1:224533/149‑1 (MQ=255)

                                                                     gAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGa                                                                                >  2:3373083/1‑149 (MQ=255)

                                                                      aaaaaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGaa                                                                               >  1:2894164/1‑149 (MQ=255)

                                                                        aaaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaag                                                                             >  2:310843/1‑149 (MQ=255)

                                                                           aCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaagaag                                                                          <  2:955890/149‑1 (MQ=255)

                                                                            cGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGc                                                                         >  2:2499092/1‑149 (MQ=255)

                                                                              cTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCa                                                                       >  2:3053742/1‑149 (MQ=255)

                                                                                ttatttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGt                                                                     >  1:2673769/1‑149 (MQ=255)

                                                                                  atttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaa                                                                   >  2:2839869/1‑149 (MQ=255)

                                                                                   tttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaaa                                                                  >  2:217213/1‑149 (MQ=255)

                                                                                   tttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaaa                                                                  >  2:1313347/1‑149 (MQ=255)

                                                                                    ttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAat                                                                 <  1:380967/149‑1 (MQ=255)

                                                                                      atCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAattg                                                               >  1:1110905/1‑149 (MQ=255)

                                                                                       tCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAattga                                                              >  2:2076315/1‑149 (MQ=255)

                                                                                          cGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAattgattg                                                           >  2:1814685/1‑149 (MQ=255)

                                                                                          cGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAattgattg                                                           >  2:3855389/1‑149 (MQ=255)

                                                                                          cGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAattgattg                                                           >  1:3526124/1‑149 (MQ=255)

                                                                                           gATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGc                                                          <  1:2358257/149‑1 (MQ=255)

                                                                                             tGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAAGGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCg                                                        >  2:3159010/1‑149 (MQ=255)

                                                                                                 aaaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCaaa                                                    >  1:251291/1‑149 (MQ=255)

                                                                                                 aaaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCaaa                                                    >  1:2017567/1‑149 (MQ=255)

                                                                                                  aaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCaaaa                                                   >  1:2417702/1‑149 (MQ=255)

                                                                                                   aTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCaaaaa                                                  <  1:863386/149‑1 (MQ=255)

                                                                                                     cGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGCCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAACCCAGTAAATTGATTGCCGCAAAAATg                                                >  2:2451661/1‑149 (MQ=255)

                                                                                                       aaTAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATgaa                                              <  1:3554001/149‑1 (MQ=255)

                                                                                                       aaTAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATgaa                                              >  2:577305/1‑149 (MQ=255)

                                                                                                         tAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATgaaga                                            >  1:2174231/1‑149 (MQ=255)

                                                                                                            aTTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAg                                         <  2:3678757/149‑1 (MQ=255)

                                                                                                                         gATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAagaaga                            >  2:3139529/1‑149 (MQ=255)

                                                                                                                          aTTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGAt                           <  2:284964/149‑1 (MQ=255)

                                                                                                                                       tAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACt              <  1:3656508/149‑1 (MQ=255)

                                                                                                                                         gTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTc            >  1:2697807/1‑149 (MQ=255)

                                                                                                                                         gTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTc            >  2:1277166/1‑149 (MQ=255)

                                                                                                                                          tttAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCa           <  2:4105971/149‑1 (MQ=255)

                                                                                                                                           ttAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCAt          <  1:1957501/149‑1 (MQ=255)

                                                                                                                                           ttAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCAt          <  1:1881409/149‑1 (MQ=255)

                                                                                                                                           ttAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCAt          <  1:3922586/149‑1 (MQ=255)

                                                                                                                                            tAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATg         <  2:2649455/149‑1 (MQ=255)

                                                                                                                                             aGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATgg        >  1:1320562/1‑149 (MQ=255)

                                                                                                                                             aGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATgg        >  2:545440/1‑149 (MQ=255)

                                                                                                                                              gCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGa       <  2:706124/149‑1 (MQ=255)

                                                                                                                                               cTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGAt      >  1:3675695/1‑149 (MQ=255)

                                                                                                                                                 gTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCg    >  2:1266289/1‑149 (MQ=255)

                                                                                                                                                  tCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGc   <  1:2161260/149‑1 (MQ=255)

                                                                                                                                                   cAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGct  >  2:3566362/1‑149 (MQ=255)

AGACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCT  >  CP009273/2531041‑2531336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`