Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,567,211 C→A 100% D152Y (GAT→TAT)  eutT ← cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,567,2110CA98.9% 838.9 / 0.1 261D152Y (GAT→TAT) eutTcobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (138/120);  total (140/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 261 total aligned reads displayed.

GAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGC  >  CP009273/2567065‑2567355
                                                                                                                                                  |                                                                                                                                                
 aGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgc                                                                                                                                               <  1:1841024/149‑1 (MQ=255)
 aGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgc                                                                                                                                               <  2:337403/149‑1 (MQ=255)
  gCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgcg                                                                                                                                              >  2:1062108/1‑149 (MQ=255)
    aaCGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgcgcg                                                                                                                                            >  2:821328/1‑149 (MQ=255)
    aaCGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgcgcg                                                                                                                                            >  2:2360741/1‑149 (MQ=255)
       gcagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCAt                                                                                                                                         >  2:2183308/1‑149 (MQ=255)
       gcagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCAt                                                                                                                                         <  2:394656/149‑1 (MQ=255)
       gcagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCAt                                                                                                                                         <  2:1019166/149‑1 (MQ=255)
       gcagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCAt                                                                                                                                         >  1:3258335/1‑149 (MQ=255)
         agcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATaa                                                                                                                                       <  2:698357/149‑1 (MQ=255)
         agcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATaa                                                                                                                                       >  1:3123549/1‑149 (MQ=255)
          gcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCAGAGGCGCGCATAAt                                                                                                                                      >  1:2785240/1‑149 (MQ=255)
             tCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGtt                                                                                                                                   <  1:1369452/149‑1 (MQ=255)
             tCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGtt                                                                                                                                   >  1:1505848/1‑149 (MQ=255)
                 gcCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCa                                                                                                                               >  2:861064/1‑149 (MQ=255)
                       ggctggctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGAcgc                                                                                                                         >  1:2640010/1‑149 (MQ=255)
                           ggctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAAc                                                                                                                     >  1:1754353/1‑149 (MQ=255)
                            gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACg                                                                                                                    >  1:4111846/1‑149 (MQ=255)
                            gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACg                                                                                                                    >  1:4111042/1‑149 (MQ=255)
                             ctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACgg                                                                                                                   <  1:1727378/149‑1 (MQ=255)
                             ctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACgg                                                                                                                   <  1:1123940/149‑1 (MQ=255)
                             cgtCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACgg                                                                                                                   <  1:1125052/147‑1 (MQ=255)
                              ttCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGa                                                                                                                  <  1:3777567/149‑1 (MQ=255)
                              ttCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGa                                                                                                                  >  1:1477462/1‑149 (MQ=255)
                              ttCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGa                                                                                                                  >  1:1425344/1‑149 (MQ=255)
                               tCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGat                                                                                                                 <  2:784899/149‑1 (MQ=255)
                                ccGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGata                                                                                                                >  2:2801709/1‑149 (MQ=255)
                                 cGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGatat                                                                                                               <  1:3029938/149‑1 (MQ=255)
                                 cGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGatat                                                                                                               <  2:1301961/149‑1 (MQ=255)
                                  ggCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATc                                                                                                              <  2:503659/149‑1 (MQ=255)
                                  ggCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATc                                                                                                              >  2:3131264/1‑149 (MQ=255)
                                  ggCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATc                                                                                                              <  1:356193/149‑1 (MQ=255)
                                  ggCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATc                                                                                                              >  1:154414/1‑149 (MQ=255)
                                  ggCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATc                                                                                                              <  2:2570157/149‑1 (MQ=255)
                                       cAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgcca                                                                                                         <  1:3058548/149‑1 (MQ=255)
                                           tgatCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgccagcca                                                                                                     >  1:46407/1‑149 (MQ=255)
                                              tCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACgg                                                                                                  >  2:3999612/1‑149 (MQ=255)
                                                gTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCt                                                                                                <  2:2639450/149‑1 (MQ=255)
                                                   gTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGcc                                                                                             <  1:1056034/149‑1 (MQ=255)
                                                   gTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGcc                                                                                             <  2:4094987/149‑1 (MQ=255)
                                                      gagaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACg                                                                                          >  1:1565638/1‑149 (MQ=255)
                                                        gaTAGCTCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                        <  2:3853015/149‑1 (MQ=255)
                                                        gaTAGCTCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                        <  2:1811147/149‑1 (MQ=255)
                                                        gaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                        <  1:3899633/149‑1 (MQ=255)
                                                          tAGCGCAGCGGCTGATGAGAAAGCCGATGCAGTTCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTc                                                                                      <  2:660391/149‑1 (MQ=255)
                                                              gcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGcc                                                                                  <  2:2954144/149‑1 (MQ=255)
                                                              gcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGcc                                                                                  <  2:3525445/149‑1 (MQ=255)
                                                              gcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGcc                                                                                  <  2:1927002/149‑1 (MQ=255)
                                                              gcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGcc                                                                                  >  1:2895449/1‑149 (MQ=255)
                                                                aGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAg                                                                                >  1:237033/1‑149 (MQ=255)
                                                                   ggCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTc                                                                             >  1:3461287/1‑149 (MQ=255)
                                                                   ggCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTc                                                                             >  2:1663161/1‑149 (MQ=255)
                                                                       gatgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAtt                                                                         >  1:1526129/1‑149 (MQ=255)
                                                                       gatgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAtt                                                                         <  1:129628/149‑1 (MQ=255)
                                                                       gatgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAtt                                                                         >  2:1228034/1‑149 (MQ=255)
                                                                        atgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAttt                                                                        <  2:1434606/149‑1 (MQ=255)
                                                                              aaGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGcc                                                                  <  2:650129/149‑1 (MQ=255)
                                                                                gCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCcac                                                                >  1:2205285/1‑149 (MQ=255)
                                                                                gCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCcac                                                                >  1:130383/1‑149 (MQ=255)
                                                                                gCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCcac                                                                >  1:37128/1‑149 (MQ=255)
                                                                                    aTGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTg                                                            >  1:3558549/1‑149 (MQ=255)
                                                                                     tGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGc                                                           >  1:985970/1‑149 (MQ=255)
                                                                                      gCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGcc                                                          >  2:1481628/1‑149 (MQ=255)
                                                                                        aGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAg                                                        >  1:3975100/1‑149 (MQ=255)
                                                                                         gATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgc                                                       <  1:2094290/149‑1 (MQ=255)
                                                                                           tCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgcgc                                                     <  1:1078029/149‑1 (MQ=255)
                                                                                             ttCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATCTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCac                                                   >  1:55990/1‑148 (MQ=255)
                                                                                                   aGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGc                                             <  1:2015070/149‑1 (MQ=255)
                                                                                                    gAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCt                                            >  1:2613501/1‑149 (MQ=255)
                                                                                                    gAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCt                                            >  1:3603330/1‑149 (MQ=255)
                                                                                                     aaaGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTg                                           >  1:2949138/1‑149 (MQ=255)
                                                                                                         cccGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCga                                       <  1:1068536/149‑1 (MQ=255)
                                                                                                             aCAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAAc                                   <  2:46407/149‑1 (MQ=255)
                                                                                                             aCAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAAc                                   >  2:3082383/1‑149 (MQ=255)
                                                                                                              cAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAAcc                                  <  1:1012423/149‑1 (MQ=255)
                                                                                                                aTCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACcgc                                >  2:1584479/1‑149 (MQ=255)
                                                                                                                     cTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaa                           <  1:1956504/149‑1 (MQ=255)
                                                                                                                     cTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaa                           >  2:428031/1‑149 (MQ=255)
                                                                                                                      tGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaaa                          >  2:1172929/1‑149 (MQ=255)
                                                                                                                        gCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAAc                        <  1:2964224/149‑1 (MQ=255)
                                                                                                                           gCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCa                     >  2:2227731/1‑149 (MQ=255)
                                                                                                                             ccAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGa                   <  1:1357636/149‑1 (MQ=255)
                                                                                                                             ccAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGa                   <  1:65915/149‑1 (MQ=255)
                                                                                                                              cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAc                  >  1:193989/1‑149 (MQ=255)
                                                                                                                              cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAc                  >  1:688346/1‑149 (MQ=255)
                                                                                                                                gCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAcgc                >  2:3068923/1‑149 (MQ=255)
                                                                                                                                 cGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAcgcg               >  2:2751712/1‑149 (MQ=255)
                                                                                                                                  ggTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCgg              <  2:1393641/149‑1 (MQ=255)
                                                                                                                                   gTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa             >  1:2503939/1‑149 (MQ=255)
                                                                                                                                   gTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa             >  2:3216186/1‑149 (MQ=255)
                                                                                                                                   gTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa             <  2:1076454/149‑1 (MQ=255)
                                                                                                                                   gTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa             >  1:884626/1‑149 (MQ=255)
                                                                                                                                    ttCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGAt            <  1:2056955/149‑1 (MQ=255)
                                                                                                                                        cccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCt        >  1:3289015/1‑149 (MQ=255)
                                                                                                                                          cAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCttt      <  2:1733304/149‑1 (MQ=255)
                                                                                                                                          cAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCttt      <  2:2785240/149‑1 (MQ=255)
                                                                                                                                             cgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTgg   <  1:529102/149‑1 (MQ=255)
                                                                                                                                             cgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTgg   <  1:1228034/149‑1 (MQ=255)
                                                                                                                                             cgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTgg   <  1:3013218/149‑1 (MQ=255)
                                                                                                                                              gcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGc  <  2:290052/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGC  >  CP009273/2567065‑2567355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑