Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,579,561 G→T 100% G158C (GGC→TGC)  narQ → sensory histidine kinase in two‑component regulatory system with NarP (NarL)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,579,5610GT99.6% 747.9 / ‑4.5 225G158C (GGC→TGC) narQsensory histidine kinase in two‑component regulatory system with NarP (NarL)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (117/107);  minor base C (0/1);  total (117/108)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

TGAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAA  >  CP009273/2579415‑2579706
                                                                                                                                                  |                                                                                                                                                 
 gAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCa                                                                                                                                                <  1:4077131/149‑1 (MQ=255)
  aataatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCAt                                                                                                                                               <  1:2526507/149‑1 (MQ=255)
  aataatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCAt                                                                                                                                               >  1:582631/1‑149 (MQ=255)
   ataatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATc                                                                                                                                              <  1:324077/149‑1 (MQ=255)
   ataatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATc                                                                                                                                              <  2:3178879/149‑1 (MQ=255)
    taatCGGCTCAGCAATGGCGATTTGCAGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCg                                                                                                                                             >  1:1546199/1‑149 (MQ=255)
     aatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCgg                                                                                                                                            >  2:1428980/1‑149 (MQ=255)
     aatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCgg                                                                                                                                            >  2:2896319/1‑149 (MQ=255)
      atCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGt                                                                                                                                           >  2:1018314/1‑149 (MQ=255)
       tCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTa                                                                                                                                          <  2:2373597/149‑1 (MQ=255)
            tCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTc                                                                                                                                     <  1:2026277/149‑1 (MQ=255)
              aGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCAc                                                                                                                                   >  1:3333975/1‑149 (MQ=255)
                  aGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTg                                                                                                                               >  1:2394180/1‑149 (MQ=255)
                  aGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTg                                                                                                                               >  1:1663861/1‑149 (MQ=255)
                   gggCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTgg                                                                                                                              <  1:303752/149‑1 (MQ=255)
                        aTTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTTATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCttt                                                                                                                         >  2:2294401/1‑149 (MQ=255)
                        aTTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCttt                                                                                                                         >  1:1201329/1‑149 (MQ=255)
                        aTTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCttt                                                                                                                         <  2:1740412/149‑1 (MQ=255)
                        aTTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCttt                                                                                                                         >  2:240939/1‑149 (MQ=255)
                               gTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACtt                                                                                                                  >  1:2480402/1‑149 (MQ=255)
                                 ggTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTg                                                                                                                <  1:3687440/149‑1 (MQ=255)
                                  gTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCTATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTgc                                                                                                               <  1:1238436/149‑1 (MQ=255)
                                      cAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGcgccg                                                                                                           >  2:2156622/1‑149 (MQ=255)
                                      cAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGcgccg                                                                                                           >  2:3057771/1‑149 (MQ=255)
                                      cAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGATGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGcgccg                                                                                                           >  2:2155922/1‑149 (MQ=255)
                                        ggCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCa                                                                                                         >  1:2159285/1‑149 (MQ=255)
                                         gCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCAt                                                                                                        <  1:3530486/149‑1 (MQ=255)
                                         gCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCAt                                                                                                        >  2:1152559/1‑149 (MQ=255)
                                           cAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATAc                                                                                                      >  1:231631/1‑149 (MQ=255)
                                            aaTATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACg                                                                                                     >  1:492680/1‑149 (MQ=255)
                                            aaTATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACg                                                                                                     >  1:1878177/1‑149 (MQ=255)
                                               atTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTAGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCa                                                                                                  <  1:3585687/149‑1 (MQ=255)
                                                  aataatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCa                                                                                               >  1:2814085/1‑149 (MQ=255)
                                                  aataatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCa                                                                                               >  2:57669/1‑149 (MQ=255)
                                                     aatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAgtt                                                                                            >  2:3886893/1‑147 (MQ=255)
                                                     aatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggt                                                                                            <  2:488355/149‑1 (MQ=255)
                                                     aatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggt                                                                                            >  1:1475218/1‑149 (MQ=255)
                                                      atTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtg                                                                                           >  1:3512350/1‑149 (MQ=255)
                                                       ttATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtgg                                                                                          >  1:2762443/1‑149 (MQ=255)
                                                        tATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtggt                                                                                         <  1:1001597/149‑1 (MQ=255)
                                                        tATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtggt                                                                                         <  1:2350479/149‑1 (MQ=255)
                                                        tATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtggt                                                                                         <  2:2192495/149‑1 (MQ=255)
                                                            ttAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGcc                                                                                     >  1:1180137/1‑149 (MQ=255)
                                                            ttAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGcc                                                                                     >  1:158216/1‑149 (MQ=255)
                                                            ttAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGcc                                                                                     >  1:2232222/1‑149 (MQ=255)
                                                             tAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGccc                                                                                    >  2:847815/1‑149 (MQ=255)
                                                              aaTCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGcccc                                                                                   >  2:906975/1‑149 (MQ=255)
                                                                tCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGc                                                                                 <  1:3002803/149‑1 (MQ=255)
                                                                tCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGc                                                                                 <  1:2450465/149‑1 (MQ=255)
                                                                tCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGc                                                                                 <  1:3334198/149‑1 (MQ=255)
                                                                  aGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTg                                                                               >  1:2257718/1‑149 (MQ=255)
                                                                   gATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGa                                                                              >  2:478570/1‑149 (MQ=255)
                                                                      aGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATc                                                                           >  1:580799/1‑149 (MQ=255)
                                                                       gACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCa                                                                          >  2:408611/1‑149 (MQ=255)
                                                                          cTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCt                                                                       <  2:4013504/149‑1 (MQ=255)
                                                                             ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGt                                                                    >  2:798260/1‑149 (MQ=255)
                                                                             ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGt                                                                    >  1:107230/1‑149 (MQ=255)
                                                                             ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGt                                                                    >  1:2335900/1‑149 (MQ=255)
                                                                             ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGt                                                                    >  1:2335332/1‑149 (MQ=255)
                                                                              tCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGtt                                                                   <  2:2394180/149‑1 (MQ=255)
                                                                               cGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTa                                                                  <  1:783675/149‑1 (MQ=255)
                                                                                gTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTAc                                                                 <  2:3654465/149‑1 (MQ=255)
                                                                                   cTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGc                                                              >  1:2931016/1‑149 (MQ=255)
                                                                                   cTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGc                                                              >  1:1187656/1‑149 (MQ=255)
                                                                                    tGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGcc                                                             >  2:2066782/1‑149 (MQ=255)
                                                                                     ggCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCa                                                            >  2:1836790/1‑149 (MQ=255)
                                                                                     ggCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCa                                                            >  2:3877390/1‑149 (MQ=255)
                                                                                     ggCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCa                                                            >  2:1836783/1‑149 (MQ=255)
                                                                                      gCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAg                                                           >  1:964930/1‑149 (MQ=255)
                                                                                       cTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGt                                                          <  2:64546/149‑1 (MQ=255)
                                                                                       cTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGt                                                          <  2:64508/149‑1 (MQ=255)
                                                                                       cTTTACAGCACTAAGCTGAAAGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATAGGTATTTTAACGCTGGTCTTTTTTACTTTTCGCCGCATACGCCATCACGTTGTTTCCCCCCTGAATCAGATGGTTACAGACAGt                                                          >  2:2829570/1‑149 (MQ=255)
                                                                                            cAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCg                                                     <  2:482776/149‑1 (MQ=255)
                                                                                             aGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGt                                                    >  1:948478/1‑149 (MQ=255)
                                                                                                aCTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCTTAtt                                                 <  2:2627667/149‑1 (MQ=255)
                                                                                                aCTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTAtt                                                 <  1:2117549/149‑1 (MQ=255)
                                                                                                aCTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTAtt                                                 <  2:2969615/149‑1 (MQ=255)
                                                                                                  tACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGa                                               <  1:1619671/149‑1 (MQ=255)
                                                                                                  tACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGa                                               >  1:1308469/1‑149 (MQ=255)
                                                                                                  tACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGa                                               <  2:1890331/149‑1 (MQ=255)
                                                                                                   aCGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGATTGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGaa                                              <  1:3547011/149‑1 (MQ=255)
                                                                                                    cGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAac                                             <  2:2242736/149‑1 (MQ=255)
                                                                                                              aaaaTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGATTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGtt                                   >  2:1677390/1‑149 (MQ=255)
                                                                                                                aaTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCg                                 <  1:1499111/149‑1 (MQ=255)
                                                                                                                  tgctgcTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGAc                               <  2:3474399/149‑1 (MQ=255)
                                                                                                                      gctgGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgc                           >  2:3265097/1‑149 (MQ=255)
                                                                                                                                 cGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGAt                <  2:171203/149‑1 (MQ=255)
                                                                                                                                 cGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGAt                <  1:255524/149‑1 (MQ=255)
                                                                                                                                 cGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGAt                <  2:171753/149‑1 (MQ=255)
                                                                                                                                 cGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGAt                <  2:2991277/149‑1 (MQ=255)
                                                                                                                                   aTTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATAc              >  1:4067344/1‑149 (MQ=255)
                                                                                                                                    tttCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATAcc             >  1:862146/1‑149 (MQ=255)
                                                                                                                                    tttCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATAcc             >  1:861794/1‑149 (MQ=255)
                                                                                                                                     ttCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCa            >  1:3814540/1‑149 (MQ=255)
                                                                                                                                        ccTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAAcc         <  2:4021566/149‑1 (MQ=255)
                                                                                                                                         ctggctggCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCt        <  2:3453808/149‑1 (MQ=255)
                                                                                                                                              tggcTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGACGCCGCTGGATACCAACCTGCCGa   >  2:1295751/1‑149 (MQ=255)
                                                                                                                                               ggctgcATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGaa  >  1:3925221/1‑149 (MQ=255)
                                                                                                                                               ggctgcATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGaa  <  1:3424471/149‑1 (MQ=255)
                                                                                                                                               ggctgcATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGaa  <  2:3248084/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAA  >  CP009273/2579415‑2579706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑