Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,669,978 C→A 100% A69A (GCG→GCT)  yphD ← inner membrane putative ABC superfamily sugar transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,669,9780CA96.2% 633.5 / 14.3 213A69A (GCG→GCT) yphDinner membrane putative ABC superfamily sugar transporter permease
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (98/107);  total (102/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 213 total aligned reads displayed.

CCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGAC  >  CP009273/2669841‑2670123
                                                                                                                                         |                                                                                                                                                 
 cTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCa                                                                                                                                       <  1:1224509/149‑1 (MQ=255)
  tAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCaa                                                                                                                                      <  1:3273651/149‑1 (MQ=255)
    aCAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATc                                                                                                                                    <  2:3334186/149‑1 (MQ=255)
      aGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAACTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCCGCGTCATAGCCCAGGCGGCAATccc                                                                                                                                  <  1:1812264/149‑1 (MQ=255)
      aGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATccc                                                                                                                                  <  1:3844899/149‑1 (MQ=255)
      aGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATccc                                                                                                                                  <  1:1812469/149‑1 (MQ=255)
         aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAt                                                                                                                               >  2:3614780/1‑149 (MQ=255)
         aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAt                                                                                                                               >  2:1278686/1‑149 (MQ=255)
          acacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtg                                                                                                                              >  2:3418049/1‑149 (MQ=255)
           cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtgg                                                                                                                             <  2:2614502/149‑1 (MQ=255)
             ccAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAAtggtg                                                                                                                           >  1:3955995/1‑149 (MQ=255)
              cagcagGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAAGTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtggtgg                                                                                                                          >  2:2401695/1‑149 (MQ=255)
               agcagGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTggc                                                                                                                         >  1:2806001/1‑149 (MQ=255)
                   ggCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCa                                                                                                                     >  2:1926545/1‑149 (MQ=255)
                   ggCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCa                                                                                                                     <  2:2576076/149‑1 (MQ=255)
                    gCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCAt                                                                                                                    <  2:3975507/149‑1 (MQ=255)
                      aCGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATcg                                                                                                                  >  1:2120170/1‑149 (MQ=255)
                                 aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt                                                                                                       >  1:1114450/1‑149 (MQ=255)
                                 aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt                                                                                                       >  1:3520801/1‑149 (MQ=255)
                                  gCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTc                                                                                                      >  1:1707499/1‑149 (MQ=255)
                                    ggAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                    >  1:3451586/1‑149 (MQ=255)
                                     gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATc                                                                                                   >  2:3883138/1‑149 (MQ=255)
                                      aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                  <  1:3418049/149‑1 (MQ=255)
                                      aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                  <  2:3945030/149‑1 (MQ=255)
                                      aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                  <  1:3702854/149‑1 (MQ=255)
                                      aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCt                                                                                                  <  2:2797595/149‑1 (MQ=255)
                                        cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTgg                                                                                                <  1:3905609/149‑1 (MQ=255)
                                         ttCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGt                                                                                               >  2:1595327/1‑149 (MQ=255)
                                             aaTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTAtt                                                                                           >  1:3747204/1‑149 (MQ=255)
                                              aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATCATCATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTc                                                                                          >  2:11651/1‑149 (MQ=255)
                                              aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTc                                                                                          >  1:2043759/1‑149 (MQ=255)
                                              aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTc                                                                                          >  2:11538/1‑149 (MQ=255)
                                                tGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCaa                                                                                        <  2:3016282/149‑1 (MQ=255)
                                                tGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCaa                                                                                        <  1:537259/149‑1 (MQ=255)
                                                    gcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                    >  1:1084925/1‑149 (MQ=255)
                                                    gcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                    <  2:3173115/149‑1 (MQ=255)
                                                    gcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                    <  2:3382145/149‑1 (MQ=255)
                                                     caAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgaga                                                                                   >  1:3664805/1‑149 (MQ=255)
                                                     caAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgaga                                                                                   <  1:2380207/149‑1 (MQ=255)
                                                      aaaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGAt                                                                                  <  1:3120603/149‑1 (MQ=255)
                                                      aaaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGAt                                                                                  <  1:3120493/149‑1 (MQ=255)
                                                      aaaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGAt                                                                                  <  1:1214054/149‑1 (MQ=255)
                                                       aaaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATa                                                                                 >  1:2236586/1‑149 (MQ=255)
                                                        aaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaa                                                                                >  2:2461042/1‑149 (MQ=255)
                                                        aaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaa                                                                                <  2:1707499/149‑1 (MQ=255)
                                                         aaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaaa                                                                               <  1:3780185/149‑1 (MQ=255)
                                                         aaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaaa                                                                               >  1:252964/1‑149 (MQ=255)
                                                          aTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAg                                                                              >  1:1414022/1‑149 (MQ=255)
                                                           tGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGc                                                                             <  1:802755/149‑1 (MQ=255)
                                                            gCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGcc                                                                            <  1:3376961/149‑1 (MQ=255)
                                                               aGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAgg                                                                         >  1:742031/1‑149 (MQ=255)
                                                                      ccGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTc                                                                  >  2:2903743/1‑149 (MQ=255)
                                                                        gagaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAg                                                                <  2:894870/149‑1 (MQ=255)
                                                                         agaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAgg                                                               <  2:2221810/149‑1 (MQ=255)
                                                                           aCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGga                                                             >  1:3831045/1‑149 (MQ=255)
                                                                             aaaaGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGgaga                                                           <  1:1623234/149‑1 (MQ=255)
                                                                               aaGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGagaag                                                         >  2:3276166/1‑149 (MQ=255)
                                                                                  ccaccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGAcc                                                      >  2:2560941/1‑149 (MQ=255)
                                                                                  ccaccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGAcc                                                      >  2:3767416/1‑149 (MQ=255)
                                                                                   caccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCa                                                     >  2:1648656/1‑149 (MQ=255)
                                                                                   caccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCa                                                     <  2:2188731/149‑1 (MQ=255)
                                                                                    accaTCGGCCCAACGCTGACATCAATTTCTCCGGAGATATTAATCAGCGTCATAGCCCAGGCGGCACTCCCACTGGTGGCGGCATCGCGCAGCACGTTCATCTTGTTATTAAATGAGATAAAGCCAGGCGCGTTATGGGAGAAGAGCAg                                                    <  2:1669171/149‑1 (MQ=255)
                                                                                    accaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAg                                                    <  2:653962/149‑1 (MQ=255)
                                                                                     ccaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGa                                                   >  2:20276/1‑149 (MQ=255)
                                                                                      caTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGAt                                                  <  2:1761931/149‑1 (MQ=255)
                                                                                           gCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGa                                             >  1:3783267/1‑149 (MQ=255)
                                                                                            cccAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGaa                                            <  1:778054/149‑1 (MQ=255)
                                                                                             ccAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAt                                           <  1:847942/149‑1 (MQ=255)
                                                                                              cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                          <  1:443730/149‑1 (MQ=255)
                                                                                              cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                          <  2:864864/149‑1 (MQ=255)
                                                                                              cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                          >  2:2076202/1‑149 (MQ=255)
                                                                                              cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                          <  1:445430/149‑1 (MQ=255)
                                                                                                aCGCTGACATCAATTTCACCGGAGATAATCCTCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                        <  2:2441503/149‑1 (MQ=255)
                                                                                                aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                        >  2:2321927/1‑149 (MQ=255)
                                                                                                aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                        >  2:1178876/1‑149 (MQ=255)
                                                                                                aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                        >  2:46559/1‑149 (MQ=255)
                                                                                                   cTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAAt                                     <  1:729522/149‑1 (MQ=255)
                                                                                                    tGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATc                                    <  1:1670325/149‑1 (MQ=255)
                                                                                                     gACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATca                                   <  1:1557078/149‑1 (MQ=255)
                                                                                                     gACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATca                                   <  2:3614085/149‑1 (MQ=255)
                                                                                                         tCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcaccac                               >  2:1051465/1‑149 (MQ=255)
                                                                                                           aaTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCACTGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAccacca                             <  2:2964042/149‑1 (MQ=255)
                                                                                                           aaTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAccacca                             >  2:4108494/1‑149 (MQ=255)
                                                                                                           aaTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCAccacca                             >  1:2579402/1‑149 (MQ=255)
                                                                                                            aTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAg                            >  2:1490996/1‑149 (MQ=255)
                                                                                                                 aCCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAc                       >  1:425902/1‑149 (MQ=255)
                                                                                                                 aCCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAc                       <  1:2398886/149‑1 (MQ=255)
                                                                                                                     gagaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAt                   <  2:3301158/149‑1 (MQ=255)
                                                                                                                      agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtc                  <  1:976278/149‑1 (MQ=255)
                                                                                                                      agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtc                  >  1:2581603/1‑149 (MQ=255)
                                                                                                                      agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtc                  >  1:4043359/1‑149 (MQ=255)
                                                                                                                        ataataATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtctc                >  1:2732672/1‑149 (MQ=255)
                                                                                                                           ataatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCAtt             >  1:1047259/1‑149 (MQ=255)
                                                                                                                            taatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTa            >  2:1182946/1‑149 (MQ=255)
                                                                                                                             aatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGATAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTaa           <  2:3664805/149‑1 (MQ=255)
                                                                                                                              atCAGCGTCATAGCCCAGGCGGCAATCCCCATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat          <  1:3891555/149‑1 (MQ=255)
                                                                                                                              atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat          >  1:2337289/1‑149 (MQ=255)
                                                                                                                              atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat          >  1:2739368/1‑149 (MQ=255)
                                                                                                                                 aGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATg       >  1:3289545/1‑149 (MQ=255)
                                                                                                                                  gCGTCATAGCCCAGGCGGCTATCCCTATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCATTGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATgg      <  2:764299/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                 
CCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGAC  >  CP009273/2669841‑2670123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑