Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,689,267 C→A 100% I36I (ATC→ATA)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,689,2670CA97.9% 778.1 / 5.4 243I36I (ATC→ATA) mltFmembrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (126/112);  total (128/115)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 251 total aligned reads displayed.

TTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGA  >  CP009273/2689121‑2689412
                                                                                                                                                  |                                                                                                                                                 
tttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCGCTCTGGCCATCCCTTCCCTGGTTTGGTAAAGCCGACAACCGGATagc                                                                                                                                                 >  1:1765327/1‑149 (MQ=255)
 ttCAAGCTGGGACGCGCACTACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGTCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATagcc                                                                                                                                                <  1:592657/149‑1 (MQ=255)
  tCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGGTTGGTAAAGCCGACAACCGTATagccg                                                                                                                                               >  2:1073058/1‑149 (MQ=255)
    aaGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAgccgcc                                                                                                                                             >  1:3260527/1‑149 (MQ=255)
      gCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCAt                                                                                                                                           >  2:1656849/1‑149 (MQ=255)
        tGGGACGCGCAAGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTc                                                                                                                                         <  2:2697885/149‑1 (MQ=255)
          gtaagcgcACGACACTGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCaa                                                                                                                                       <  2:395008/145‑1 (MQ=255)
                       acaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGt                                                                                                                          <  2:2586183/149‑1 (MQ=255)
                       acaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGt                                                                                                                          >  2:2186891/1‑149 (MQ=255)
                              aTTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgtgt                                                                                                                   >  2:2061124/1‑149 (MQ=255)
                              aTTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgtgt                                                                                                                   >  1:236844/1‑149 (MQ=255)
                              aTTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgtgt                                                                                                                   >  1:620862/1‑149 (MQ=255)
                              aTTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgtgt                                                                                                                   >  1:1249891/1‑149 (MQ=255)
                                tAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGa                                                                                                                 <  1:3871443/149‑1 (MQ=255)
                                 aaCTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAg                                                                                                                >  1:1427272/1‑149 (MQ=255)
                                    tAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCAc                                                                                                             >  2:1886442/1‑149 (MQ=255)
                                     aaTTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCAcc                                                                                                            >  1:1348672/1‑149 (MQ=255)
                                          aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCa                                                                                                       >  1:937633/1‑149 (MQ=255)
                                          aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCAACCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCa                                                                                                       >  1:1060858/1‑149 (MQ=255)
                                           aaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCAt                                                                                                      <  2:137042/149‑1 (MQ=255)
                                            aaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATa                                                                                                     <  1:2245131/149‑1 (MQ=255)
                                            aaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATa                                                                                                     >  1:571672/1‑149 (MQ=255)
                                             aaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGTCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATAc                                                                                                    <  2:3531442/149‑1 (MQ=255)
                                                 tAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTccc                                                                                                >  2:90605/1‑149 (MQ=255)
                                                 tAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTAATACTccc                                                                                                <  1:1120100/149‑1 (MQ=255)
                                                  aaaGTTTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                                                                                               >  2:3037704/1‑149 (MQ=255)
                                                  aaaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                                                                                               >  2:1949123/1‑149 (MQ=255)
                                                  aaaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAACCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                                                                                               >  2:3070764/1‑149 (MQ=255)
                                                   aaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCt                                                                                              >  2:2750776/1‑149 (MQ=255)
                                                   aaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTCGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCt                                                                                              <  1:2422751/149‑1 (MQ=255)
                                                   aaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCGGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCt                                                                                              >  1:1937319/1‑149 (MQ=255)
                                                      attaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGAc                                                                                           <  2:1493292/149‑1 (MQ=255)
                                                      attaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGAc                                                                                           <  2:1353968/149‑1 (MQ=255)
                                                       ttaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACt                                                                                          <  2:3766768/149‑1 (MQ=255)
                                                          attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtat                                                                                       <  1:3104207/149‑1 (MQ=255)
                                                           ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCCTTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCAGACTtata                                                                                      >  1:213972/1‑149 (MQ=255)
                                                           ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCCCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtata                                                                                      >  1:213877/1‑149 (MQ=255)
                                                           ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCAGGCCATCCATTACCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCAGACTtata                                                                                      >  1:3158941/1‑149 (MQ=255)
                                                             aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAAc                                                                                    >  2:1207377/1‑149 (MQ=255)
                                                              tCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGGATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGGGTGAGCACCATTCATACTCCCCTGACTTATAACg                                                                                   >  1:1612446/1‑149 (MQ=255)
                                                               cTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCAAACCATTCCCTGGTGTGGTAAAGCCGACAACCCTATATCCGCCATTCAACCCCGGGGAGAGTTGCGTGTGATCACAATTCATACTACCCAGACATAAAAcaa                                                                                  >  1:3685144/1‑147 (MQ=255)
                                                                tGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGaa                                                                                 >  2:167660/1‑149 (MQ=255)
                                                                  ttCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAAt                                                                               >  2:2800746/1‑149 (MQ=255)
                                                                  ttCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAAt                                                                               >  1:3217467/1‑149 (MQ=255)
                                                                   tCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATc                                                                              >  2:2399103/1‑149 (MQ=255)
                                                                     aTCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCaa                                                                            >  2:82632/1‑149 (MQ=255)
                                                                     aTCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCAGACGTATAACGAAATCaa                                                                            >  1:2504825/1‑149 (MQ=255)
                                                                     aTCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCAATTCAAGAGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCaa                                                                            >  2:931196/1‑149 (MQ=255)
                                                                      tCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAAc                                                                           >  2:3216035/1‑149 (MQ=255)
                                                                      tCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCGCTGGCCCTCCATTACCTGGTTTGGTAAAGCCGACAACCGTATATCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAAc                                                                           >  1:70645/1‑149 (MQ=255)
                                                                        ggCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACgg                                                                         >  2:59967/1‑149 (MQ=255)
                                                                         gCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACggg                                                                        >  1:1725355/1‑149 (MQ=255)
                                                                         gCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACggg                                                                        >  1:1562908/1‑149 (MQ=255)
                                                                          cATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGa                                                                       <  1:2926875/149‑1 (MQ=255)
                                                                               tGGCACTGCTGCTCGCGGTCTCTCTCTGGCCATCCATTCACTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCt                                                                  <  2:2623746/149‑1 (MQ=255)
                                                                               tGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTTGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCt                                                                  <  2:697795/149‑1 (MQ=255)
                                                                                 gCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAAACAACGGGAAACCgtt                                                                >  1:435483/1‑149 (MQ=255)
                                                                                   aCTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTg                                                              <  2:105740/149‑1 (MQ=255)
                                                                                    ctgctgCTCGCGGTCGCTCTCTGGCCCTCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCAATCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTgg                                                             >  1:68101/1‑149 (MQ=255)
                                                                                             gcgGTCGCTCTCTGGCCATCCATTCCCTGGTTTTGTAAATCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCAGACTTATAACGAAATAAACGGGAAACCTTTTGGCCTGGATTa                                                    >  1:2566346/1‑149 (MQ=255)
                                                                                             gcgGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTa                                                    >  1:2030649/1‑149 (MQ=255)
                                                                                             gcgGTCGCTCTCTGGCCATCCAGTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTa                                                    <  2:3845590/149‑1 (MQ=255)
                                                                                                gTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGa                                                 >  1:2653579/1‑149 (MQ=255)
                                                                                                 tCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGaa                                                <  1:2467547/149‑1 (MQ=255)
                                                                                                  cgatctctGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAAc                                               <  2:4089525/146‑1 (MQ=255)
                                                                                                  cGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAAc                                               <  1:1109900/149‑1 (MQ=255)
                                                                                                   gCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACt                                              <  1:1645321/149‑1 (MQ=255)
                                                                                                    ctctctGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTg                                             <  1:2764631/149‑1 (MQ=255)
                                                                                                      ctctGGCCAGCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGc                                           <  1:3064932/149‑1 (MQ=255)
                                                                                                            ccatccatTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACa                                     <  1:779588/149‑1 (MQ=255)
                                                                                                                ccatTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGttt                                 <  1:1367943/149‑1 (MQ=255)
                                                                                                                  atTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGc                               >  1:1094122/1‑149 (MQ=255)
                                                                                                                  atTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGc                               <  2:1622303/149‑1 (MQ=255)
                                                                                                                   ttCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGcc                              >  2:2385147/1‑149 (MQ=255)
                                                                                                                   ttCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGcc                              >  1:397488/1‑149 (MQ=255)
                                                                                                                     cccTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGa                            <  2:3115518/149‑1 (MQ=255)
                                                                                                                     agcTGTTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGa                            <  2:4035353/147‑1 (MQ=255)
                                                                                                                       cttgTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGAGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAtt                          <  2:1264013/146‑1 (MQ=255)
                                                                                                                       cTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAtt                          <  2:874464/149‑1 (MQ=255)
                                                                                                                       cTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAtt                          <  1:2730258/149‑1 (MQ=255)
                                                                                                                         ggTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTAc                        <  1:2183162/149‑1 (MQ=255)
                                                                                                                          gTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTAcc                       <  2:96868/149‑1 (MQ=255)
                                                                                                                            ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTc                     <  1:3584870/149‑1 (MQ=255)
                                                                                                                            ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTc                     <  1:507252/149‑1 (MQ=255)
                                                                                                                            ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTc                     <  2:158086/149‑1 (MQ=255)
                                                                                                                                tAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAGGGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCg                 >  2:341447/1‑149 (MQ=255)
                                                                                                                                 aaaGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAAAGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGt                <  2:2449197/149‑1 (MQ=255)
                                                                                                                                     ccGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaaaa            >  1:2845420/1‑149 (MQ=255)
                                                                                                                                     ccGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaaaa            <  2:394612/149‑1 (MQ=255)
                                                                                                                                       gACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACt          <  2:36148/149‑1 (MQ=255)
                                                                                                                                        aCAACCGTACAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCCCGGCGTAAAACTg         <  1:921862/149‑1 (MQ=255)
                                                                                                                                         cAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACTAAATCAACGGGAAACCTTTTGGCATGGATTACGAAATGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGa        <  2:3059490/149‑1 (MQ=255)
                                                                                                                                           aCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaaa      <  1:896429/149‑1 (MQ=255)
                                                                                                                                           aCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaaa      <  2:2506688/149‑1 (MQ=255)
                                                                                                                                             cGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGt    >  2:2915816/1‑149 (MQ=255)
                                                                                                                                             cGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGt    <  1:849899/149‑1 (MQ=255)
                                                                                                                                             cGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGt    <  1:1660793/149‑1 (MQ=255)
                                                                                                                                              gTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTg   >  2:3692481/1‑149 (MQ=255)
                                                                                                                                              gTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTg   >  1:3470779/1‑149 (MQ=255)
                                                                                                                                               tataGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGTCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGa  >  1:558685/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGA  >  CP009273/2689121‑2689412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑