Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,692,793 C→A 100% H226N (CAT→AAT)  yfhH → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,692,7930CA99.2% 799.4 / ‑2.0 244H226N (CAT→AAT) yfhHputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (113/129);  total (114/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 246 total aligned reads displayed.

CGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCATAG  >  CP009273/2692647‑2692938
                                                                                                                                                  |                                                                                                                                                 
cgtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAAt                                                                                                                                                 <  1:481083/149‑1 (MQ=255)
 gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAAtt                                                                                                                                                <  2:2482963/149‑1 (MQ=255)
   ccccTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGc                                                                                                                                              <  2:242833/149‑1 (MQ=255)
      cTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTg                                                                                                                                           >  2:2753255/1‑149 (MQ=255)
        gACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGta                                                                                                                                         <  1:3083831/149‑1 (MQ=255)
        gACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGta                                                                                                                                         >  1:561164/1‑149 (MQ=255)
         aCGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGtat                                                                                                                                        >  1:4022186/1‑149 (MQ=255)
         aCGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGtat                                                                                                                                        <  2:3185861/149‑1 (MQ=255)
           gATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATAc                                                                                                                                      <  2:1982305/149‑1 (MQ=255)
             tCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCa                                                                                                                                    >  2:2630442/1‑149 (MQ=255)
              cTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCAt                                                                                                                                   <  2:584206/149‑1 (MQ=255)
              cTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCAt                                                                                                                                   >  2:3731635/1‑149 (MQ=255)
               tGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCAtt                                                                                                                                  <  1:419719/149‑1 (MQ=255)
               tGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCAtt                                                                                                                                  <  2:2582056/149‑1 (MQ=255)
               tGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCAtt                                                                                                                                  <  2:3453400/149‑1 (MQ=255)
                 ttattaGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGc                                                                                                                                <  2:677076/149‑1 (MQ=255)
                 ttattaGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGc                                                                                                                                <  2:3801486/149‑1 (MQ=255)
                    ttaGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCga                                                                                                                             >  1:252401/1‑149 (MQ=255)
                      aGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCgaag                                                                                                                           <  2:3817378/149‑1 (MQ=255)
                         cATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAAc                                                                                                                        <  1:3658009/149‑1 (MQ=255)
                         cATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAAc                                                                                                                        >  1:1088384/1‑149 (MQ=255)
                          aTTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACa                                                                                                                       >  1:1084652/1‑149 (MQ=255)
                           tttCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACaa                                                                                                                      <  2:3713453/149‑1 (MQ=255)
                           tttCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACaa                                                                                                                      <  1:4107737/149‑1 (MQ=255)
                             tCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGGGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGc                                                                                                                    <  2:1835743/149‑1 (MQ=255)
                             tCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGc                                                                                                                    >  2:3128059/1‑149 (MQ=255)
                             tCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGc                                                                                                                    <  2:1856040/149‑1 (MQ=255)
                               cTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGa                                                                                                                  >  2:120680/1‑149 (MQ=255)
                                    ccGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAAc                                                                                                             <  1:2316451/149‑1 (MQ=255)
                                    ccGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAAc                                                                                                             <  2:685466/149‑1 (MQ=255)
                                      ggTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAg                                                                                                           <  2:2620074/149‑1 (MQ=255)
                                      ggTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAg                                                                                                           <  2:1565900/149‑1 (MQ=255)
                                         gtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGc                                                                                                        >  1:2288788/1‑149 (MQ=255)
                                           acgacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCtt                                                                                                      >  1:578750/1‑149 (MQ=255)
                                              acgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCaa                                                                                                   <  1:615212/149‑1 (MQ=255)
                                              acgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCaa                                                                                                   <  2:4018857/149‑1 (MQ=255)
                                                 cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAtct                                                                                                <  1:1709652/149‑1 (MQ=255)
                                                     ttAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                                                            >  1:961459/1‑149 (MQ=255)
                                                       aaaCCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCtt                                                                                          <  2:422627/149‑1 (MQ=255)
                                                       aaaCCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCtt                                                                                          >  1:1777830/1‑149 (MQ=255)
                                                       aaaCCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCtt                                                                                          >  2:680498/1‑149 (MQ=255)
                                                         aCCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGt                                                                                        >  2:1675581/1‑149 (MQ=255)
                                                          ccTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTc                                                                                       <  1:558642/149‑1 (MQ=255)
                                                           cTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCa                                                                                      >  1:3047114/1‑149 (MQ=255)
                                                            tGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCAc                                                                                     <  2:3952029/149‑1 (MQ=255)
                                                            tGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCAc                                                                                     <  2:3951560/149‑1 (MQ=255)
                                                            tGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCAc                                                                                     <  2:2445929/149‑1 (MQ=255)
                                                             ggcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACg                                                                                    >  1:2964341/1‑149 (MQ=255)
                                                                ggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTc                                                                                 <  1:3400292/149‑1 (MQ=255)
                                                                 gcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCa                                                                                <  2:1955443/149‑1 (MQ=255)
                                                                   agatgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAgg                                                                              <  2:957603/149‑1 (MQ=255)
                                                                   agatgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAgg                                                                              <  1:64705/149‑1 (MQ=255)
                                                                   agatgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAgg                                                                              <  1:1860540/149‑1 (MQ=255)
                                                                    gatgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAggg                                                                             <  1:2406679/149‑1 (MQ=255)
                                                                    gatgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAggg                                                                             >  1:3256654/1‑149 (MQ=255)
                                                                      tgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGaa                                                                           <  1:3155764/149‑1 (MQ=255)
                                                                        agatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATg                                                                         >  2:1331810/1‑149 (MQ=255)
                                                                           tgctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCAACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTa                                                                      >  2:866185/1‑149 (MQ=255)
                                                                            gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTaa                                                                     >  1:4051966/1‑149 (MQ=255)
                                                                            gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTaa                                                                     <  2:2342502/149‑1 (MQ=255)
                                                                             ctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAAc                                                                    >  1:1815747/1‑149 (MQ=255)
                                                                              tgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACg                                                                   <  1:3057262/149‑1 (MQ=255)
                                                                                   gTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGAttt                                                              <  2:1286984/149‑1 (MQ=255)
                                                                                           aaaaGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCa                                                      <  1:2753255/149‑1 (MQ=255)
                                                                                             aaGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCAtt                                                    >  1:2778359/1‑149 (MQ=255)
                                                                                               gTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTgc                                                  >  1:3597038/1‑149 (MQ=255)
                                                                                                 gCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTgcgc                                                <  2:1088384/149‑1 (MQ=255)
                                                                                                    ggCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGa                                             >  2:3582605/1‑149 (MQ=255)
                                                                                                    ggCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGa                                             >  2:1535961/1‑149 (MQ=255)
                                                                                                    ggCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGa                                             <  2:1733530/149‑1 (MQ=255)
                                                                                                       gATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTc                                          >  1:3176019/1‑149 (MQ=255)
                                                                                                        aTTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTca                                         >  1:2157068/1‑149 (MQ=255)
                                                                                                          tACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagc                                       >  1:3779406/1‑149 (MQ=255)
                                                                                                           aCCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagca                                      <  1:707116/149‑1 (MQ=255)
                                                                                                            ccGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagcag                                     >  1:1720431/1‑149 (MQ=255)
                                                                                                              ggCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGa                                   >  2:1565125/1‑149 (MQ=255)
                                                                                                               gCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGAt                                  >  1:2435489/1‑149 (MQ=255)
                                                                                                                 tttACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCt                                <  2:252401/149‑1 (MQ=255)
                                                                                                                 tttACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCt                                <  1:3474703/149‑1 (MQ=255)
                                                                                                                     cTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGaa                            >  1:2578884/1‑149 (MQ=255)
                                                                                                                     cTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGaa                            >  1:1909475/1‑149 (MQ=255)
                                                                                                                       ccGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACt                          <  1:146056/149‑1 (MQ=255)
                                                                                                                         gAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTgg                        <  1:2073774/149‑1 (MQ=255)
                                                                                                                           aTGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCa                      <  2:2753842/149‑1 (MQ=255)
                                                                                                                            tGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCAc                     >  2:3346028/1‑149 (MQ=255)
                                                                                                                            tGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCAc                     <  1:2797407/149‑1 (MQ=255)
                                                                                                                             gCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCAcc                    <  1:3810665/149‑1 (MQ=255)
                                                                                                                               ccTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAg                  >  1:251608/1‑149 (MQ=255)
                                                                                                                                 tGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGaa                <  2:3562761/149‑1 (MQ=255)
                                                                                                                                 tGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGaa                <  2:2964341/149‑1 (MQ=255)
                                                                                                                                  gCAACAGCGTGCTTCTAATTGCATGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAAc               <  1:1148553/149‑1 (MQ=255)
                                                                                                                                     aCAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTa            >  2:4056572/1‑149 (MQ=255)
                                                                                                                                        gCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTc         <  1:2110893/149‑1 (MQ=255)
                                                                                                                                        gCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTc         >  1:1268834/1‑149 (MQ=255)
                                                                                                                                         cGTGCTTCTAAGTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCg        <  2:3100426/149‑1 (MQ=255)
                                                                                                                                          gTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGt       >  2:1391676/1‑149 (MQ=255)
                                                                                                                                           tGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTc      >  2:1881061/1‑149 (MQ=255)
                                                                                                                                            gCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCa     >  2:1529003/1‑149 (MQ=255)
                                                                                                                                            gCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCAACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCa     <  1:866185/149‑1 (MQ=255)
                                                                                                                                             cTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCAt    >  2:523990/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCATAG  >  CP009273/2692647‑2692938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑