Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,755 C→A 100% D45Y (GAC→TAC)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,7550CA98.1% 872.9 / 5.4 273D45Y (GAC→TAC) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base A (126/141);  total (128/145)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 273 total aligned reads displayed.

CTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACC  >  CP009273/2711610‑2711900
                                                                                                                                                 |                                                                                                                                                 
cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGc                                                                                                                                                >  1:3032908/1‑149 (MQ=255)
 ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCa                                                                                                                                               >  1:2288312/1‑149 (MQ=255)
      ccACTGAGGCCCCCTTGACCGGCCTTTTCCGCCGCCACAGCACCCGGCGCGCGGTAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACgaga                                                                                                                                          <  2:846219/149‑1 (MQ=255)
       cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAt                                                                                                                                         >  2:688185/1‑149 (MQ=255)
        aCTGAGGCCACCTTTCTCGGCTTTTCCGGGCGTCCCACCCCCCGGCGCGCGGAAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtt                                                                                                                                        <  2:795773/149‑1 (MQ=255)
         cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAttt                                                                                                                                       >  1:2860300/1‑149 (MQ=255)
          tGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtttt                                                                                                                                      >  2:1491484/1‑149 (MQ=255)
            aTGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTcc                                                                                                                                    >  2:2781652/1‑149 (MQ=255)
             tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGACACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCt                                                                                                                                   >  2:3575912/1‑149 (MQ=255)
             cgCCACCGTGATCGGCCTTGCCCGGCGTCTAACCCCCCGGCGCGCGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCt                                                                                                                                   <  2:1197424/148‑1 (MQ=255)
               cccccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTgg                                                                                                                                 >  2:2061169/4‑149 (MQ=255)
                 aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAc                                                                                                                               >  1:1061206/1‑149 (MQ=255)
                 aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAc                                                                                                                               >  2:2269400/1‑149 (MQ=255)
                  ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcg                                                                                                                              >  1:1894213/1‑149 (MQ=255)
                  ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcg                                                                                                                              >  2:1753138/1‑149 (MQ=255)
                  ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcg                                                                                                                              >  2:4000101/1‑149 (MQ=255)
                  ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGACCACCGTAAGCACGAGATTTTCCTGGAcg                                                                                                                              >  1:260637/1‑149 (MQ=255)
                        tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcgcccgcc                                                                                                                        >  1:3782825/1‑149 (MQ=255)
                           gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGccccc                                                                                                                     >  1:3516308/1‑149 (MQ=255)
                            ccTTTTCGGGCGAATCATCACCCGGCGCGCGGCAAACGGTGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCt                                                                                                                    <  1:3407009/149‑1 (MQ=255)
                            ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCt                                                                                                                    >  1:3341791/1‑149 (MQ=255)
                              ttttCCGGCGTCTCATCCCCCGGCGCGGGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCttt                                                                                                                  <  2:2583789/149‑1 (MQ=255)
                              ttgtCCGGCGTCACATCACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCttt                                                                                                                  <  2:516199/146‑1 (MQ=255)
                                tgcgggCGTCTAACCCCCGGGCGCGCGGTACCCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTcc                                                                                                                <  1:585670/145‑1 (MQ=255)
                                  ccGGCGTCTCCTCCCCCGGCGGGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCg                                                                                                              <  2:3514433/149‑1 (MQ=255)
                                     gCGTCTCATCCCCCGGCGCGCGGGAAACGGGCCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTa                                                                                                           <  2:2471847/149‑1 (MQ=255)
                                          tcatcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCgg                                                                                                      >  1:3659885/1‑149 (MQ=255)
                                          tcaacaCCCGGCGCGCGGCAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCgg                                                                                                      <  2:3071709/149‑1 (MQ=255)
                                           catcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                                                                                                     >  2:2498036/1‑149 (MQ=255)
                                                 cgggcgggcgGCACACCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTACTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                                                                                               <  1:1665346/147‑1 (MQ=255)
                                                 ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGCCGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                                                                                               >  2:2155591/1‑149 (MQ=255)
                                                 ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                                                                                               <  2:3185991/149‑1 (MQ=255)
                                                   ggCGCGCGGGAAACCGTGAGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGt                                                                                             >  2:517093/1‑149 (MQ=255)
                                                   ggCGCGCGGGAAACCGTGAGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGt                                                                                             >  2:2654244/1‑149 (MQ=255)
                                                        gggggAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGtt                                                                                        <  2:2416609/147‑1 (MQ=255)
                                                         cgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGttt                                                                                       <  2:1006750/149‑1 (MQ=255)
                                                           ggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt                                                                                     >  1:1947750/1‑149 (MQ=255)
                                                           ggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt                                                                                     >  1:1350052/1‑149 (MQ=255)
                                                            gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc                                                                                    >  2:2466801/1‑149 (MQ=255)
                                                             aaaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa                                                                                   >  2:3285314/1‑149 (MQ=255)
                                                               aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                                                                 >  2:1660157/1‑149 (MQ=255)
                                                                ccGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGaa                                                                                <  2:3341791/149‑1 (MQ=255)
                                                                ccGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGaa                                                                                <  1:2509585/149‑1 (MQ=255)
                                                                 cGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAat                                                                               <  1:3199210/149‑1 (MQ=255)
                                                                    gcgcCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcat                                                                            <  1:1193181/149‑1 (MQ=255)
                                                                      gcCACGGCGAATCTTCCCACCTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatca                                                                          <  2:3845857/149‑1 (MQ=255)
                                                                      cccACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatca                                                                          <  1:179197/148‑1 (MQ=255)
                                                                         aCGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                       >  2:3030053/1‑149 (MQ=255)
                                                                         aCGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                       >  2:888818/1‑149 (MQ=255)
                                                                         aCGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                       <  1:1859986/149‑1 (MQ=255)
                                                                          cGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCAc                                                                      <  1:2802458/149‑1 (MQ=255)
                                                                             cGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGc                                                                   >  2:2965478/1‑149 (MQ=255)
                                                                              gAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCg                                                                  >  1:3683593/1‑149 (MQ=255)
                                                                               aaTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                                                                 <  1:221324/149‑1 (MQ=255)
                                                                               aaTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                                                                 >  2:2803299/1‑149 (MQ=255)
                                                                                aTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGa                                                                <  2:1066436/149‑1 (MQ=255)
                                                                                 tCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGac                                                               <  1:2894350/149‑1 (MQ=255)
                                                                                    tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacata                                                            <  1:827551/149‑1 (MQ=255)
                                                                                          ttGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCAc                                                      >  1:350642/1‑149 (MQ=255)
                                                                                            gCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACtt                                                    <  2:1206695/149‑1 (MQ=255)
                                                                                             cGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACttt                                                   <  1:2713568/149‑1 (MQ=255)
                                                                                             cGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACttt                                                   <  1:236518/149‑1 (MQ=255)
                                                                                             cGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACttt                                                   <  1:994576/149‑1 (MQ=255)
                                                                                                tCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAc                                                <  1:2449282/149‑1 (MQ=255)
                                                                                                tCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAc                                                >  1:1819708/1‑149 (MQ=255)
                                                                                                 cACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAcc                                               <  1:1716014/149‑1 (MQ=255)
                                                                                                  aCGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCt                                              >  2:734604/1‑149 (MQ=255)
                                                                                                    ggggCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACCTACATCACTTTGACCTTg                                            >  2:2318157/1‑149 (MQ=255)
                                                                                                      ggCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGcc                                          >  2:2340972/1‑149 (MQ=255)
                                                                                                        cTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGc                                        <  1:1337007/149‑1 (MQ=255)
                                                                                                          gACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGTGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                      <  1:1488355/149‑1 (MQ=255)
                                                                                                          gACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                      >  1:2581064/1‑149 (MQ=255)
                                                                                                          gACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                      >  2:614762/1‑149 (MQ=255)
                                                                                                           aCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCttt                                     <  1:2025976/149‑1 (MQ=255)
                                                                                                             tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCttttt                                   >  1:3149719/1‑148 (MQ=255)
                                                                                                              ctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAc                                  <  1:1581432/149‑1 (MQ=255)
                                                                                                              ctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAc                                  >  2:1146010/1‑149 (MQ=255)
                                                                                                               tGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAcc                                 >  1:2666546/1‑149 (MQ=255)
                                                                                                               tGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAcc                                 >  2:4083159/1‑149 (MQ=255)
                                                                                                                gTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCt                                <  2:3659885/149‑1 (MQ=255)
                                                                                                                  ttGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCttt                              <  2:3516308/149‑1 (MQ=255)
                                                                                                                   tGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttc                             <  1:929475/149‑1 (MQ=255)
                                                                                                                   tGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttc                             >  2:3416034/1‑149 (MQ=255)
                                                                                                                    gTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttca                            <  2:1758143/149‑1 (MQ=255)
                                                                                                                      cATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCCCTTTGACCTTGCCGCTTTTACCtttcatt                          <  2:2869716/149‑1 (MQ=255)
                                                                                                                      cATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATAATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcatt                          <  1:3579625/149‑1 (MQ=255)
                                                                                                                       aTCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcattt                         <  2:1900316/149‑1 (MQ=255)
                                                                                                                        tCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcatttc                        <  2:2181065/149‑1 (MQ=255)
                                                                                                                        tCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcatttc                        >  1:4026913/1‑149 (MQ=255)
                                                                                                                        tCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcatttc                        >  1:561912/1‑149 (MQ=255)
                                                                                                                         cgcgGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcatttca                       <  1:3540543/149‑1 (MQ=255)
                                                                                                                          gcgggcggGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTttcatttcat                      <  2:1093836/149‑1 (MQ=255)
                                                                                                                              gcggggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGtt                  <  1:397055/149‑1 (MQ=255)
                                                                                                                               cggggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTc                 <  2:1045276/149‑1 (MQ=255)
                                                                                                                                  ggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATg              <  2:1211941/149‑1 (MQ=255)
                                                                                                                                     gACGGCCACAGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCtt           <  2:3129473/149‑1 (MQ=255)
                                                                                                                                         gCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTtct       <  1:4097566/149‑1 (MQ=255)
                                                                                                                                          ccACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGGTCATGCTTTtctc      >  1:1168655/1‑149 (MQ=255)
                                                                                                                                          ccACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGAGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTtgtc      >  1:3877188/1‑149 (MQ=255)
                                                                                                                                           cACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTcc     >  1:3273620/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACC  >  CP009273/2711610‑2711900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑