Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,778,820 G→A 100% intergenic (‑450/+301) ypjC ← / ← ileY pseudogene/tRNA‑Ile

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,778,8200GA97.9% 929.8 / 7.0 280intergenic (‑450/+301)ypjC/ileYpseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base A (124/150);  total (126/154)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 285 total aligned reads displayed.

GCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGA  >  CP009273/2778674‑2778963
                                                                                                                                                  |                                                                                                                                               
gcctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATa                                                                                                                                               >  1:522919/1‑149 (MQ=255)
gcctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATa                                                                                                                                               <  2:2567734/149‑1 (MQ=255)
gcctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATa                                                                                                                                               >  1:132960/1‑149 (MQ=255)
gcctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATa                                                                                                                                               <  2:2680304/149‑1 (MQ=255)
  ctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGaataat                                                                                                                                             >  2:1998812/1‑149 (MQ=255)
  ctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGaataat                                                                                                                                             <  1:2570717/149‑1 (MQ=255)
  ctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGaataat                                                                                                                                             <  1:2285890/149‑1 (MQ=255)
     cggcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACTTTGCTTGTGGTACTCACTGATCAGCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTaa                                                                                                                                          <  1:4034770/149‑1 (MQ=255)
     cggcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTaa                                                                                                                                          <  2:2907087/149‑1 (MQ=255)
     cggcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTaa                                                                                                                                          <  1:1014646/149‑1 (MQ=255)
        cggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCt                                                                                                                                       <  2:578098/149‑1 (MQ=255)
        cggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCt                                                                                                                                       <  2:2739654/149‑1 (MQ=255)
        cggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCt                                                                                                                                       <  2:578048/149‑1 (MQ=255)
          ggTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGa                                                                                                                                     <  2:725817/149‑1 (MQ=255)
           gtgtTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGAt                                                                                                                                    >  2:786079/1‑149 (MQ=255)
              ttCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTaa                                                                                                                                 <  2:3631265/149‑1 (MQ=255)
              ttCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTaa                                                                                                                                 >  2:2344677/1‑149 (MQ=255)
               tCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTaaa                                                                                                                                <  2:1821273/149‑1 (MQ=255)
                cTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAAt                                                                                                                               <  2:3041036/149‑1 (MQ=255)
                    tCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAg                                                                                                                           >  2:2289613/1‑149 (MQ=255)
                     cAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAgg                                                                                                                          <  1:3113551/149‑1 (MQ=255)
                      agtagtAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAggg                                                                                                                         >  2:2075309/1‑149 (MQ=255)
                       gtagtaGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAgggg                                                                                                                        <  2:3278222/149‑1 (MQ=255)
                        tagtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGt                                                                                                                       >  1:1095066/1‑149 (MQ=255)
                         agtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGta                                                                                                                      <  2:690600/149‑1 (MQ=255)
                           tagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGtata                                                                                                                    >  2:3621035/1‑149 (MQ=255)
                             gATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTataat                                                                                                                  >  1:847061/1‑149 (MQ=255)
                             gATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTataat                                                                                                                  >  1:2104141/1‑149 (MQ=255)
                               tGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTAtaataa                                                                                                                >  2:3399915/1‑149 (MQ=255)
                               tGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTAtaataa                                                                                                                >  2:3936840/1‑149 (MQ=255)
                                gTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATaaa                                                                                                               >  2:927036/1‑149 (MQ=255)
                                gTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATaaa                                                                                                               <  1:3841081/149‑1 (MQ=255)
                                gTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATaaa                                                                                                               >  2:3829664/1‑149 (MQ=255)
                                    aaGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGat                                                                                                           >  2:2546159/1‑149 (MQ=255)
                                    aaGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGat                                                                                                           <  2:3160775/149‑1 (MQ=255)
                                      ggCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGataa                                                                                                         >  2:200668/1‑149 (MQ=255)
                                        cGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGataata                                                                                                       <  2:2823815/149‑1 (MQ=255)
                                         gTGACAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATAc                                                                                                      >  1:2972633/1‑149 (MQ=255)
                                             cAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTg                                                                                                  >  2:2612023/1‑149 (MQ=255)
                                                agaCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCt                                                                                               <  2:3911782/149‑1 (MQ=255)
                                                 gaCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCtt                                                                                              <  1:2040154/149‑1 (MQ=255)
                                                  aCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCttt                                                                                             <  1:34200/149‑1 (MQ=255)
                                                       tCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGt                                                                                        >  1:3712731/1‑149 (MQ=255)
                                                       tCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGt                                                                                        <  1:1528353/149‑1 (MQ=255)
                                                         aTCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTa                                                                                      <  1:3525501/149‑1 (MQ=255)
                                                          tCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTaa                                                                                     >  2:1866231/1‑149 (MQ=255)
                                                          tCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTaa                                                                                     >  1:574853/1‑149 (MQ=255)
                                                           ccTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAAt                                                                                    <  1:1432229/149‑1 (MQ=255)
                                                            cTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAAtt                                                                                   >  2:43274/1‑149 (MQ=255)
                                                             ttACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTa                                                                                  >  1:2752580/1‑149 (MQ=255)
                                                                ctctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACtt                                                                               <  2:3518148/149‑1 (MQ=255)
                                                                    aCGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAAtt                                                                           <  1:3362902/149‑1 (MQ=255)
                                                                    aCGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAAtt                                                                           <  1:3196587/149‑1 (MQ=255)
                                                                     cGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTc                                                                          <  2:848441/149‑1 (MQ=255)
                                                                     cGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTc                                                                          <  1:955867/149‑1 (MQ=255)
                                                                         aTTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCcaca                                                                      <  2:3244749/149‑1 (MQ=255)
                                                                         aTTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCcaca                                                                      >  1:2636811/1‑149 (MQ=255)
                                                                          ttGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACAt                                                                     <  1:4071315/149‑1 (MQ=255)
                                                                          ttGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACAt                                                                     >  2:3476776/1‑149 (MQ=255)
                                                                           tGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATg                                                                    <  2:1501805/149‑1 (MQ=255)
                                                                              tCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTaa                                                                 <  1:1150631/149‑1 (MQ=255)
                                                                                tacatacaTTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGc                                                               >  1:3303880/1‑149 (MQ=255)
                                                                                tacatacaGTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTACAGTGATTTGAATAATTAACCTGATTAAATGAAGGTGTATAATAATTGATAATACTCTGTCTTTATCGTTAATTACTTAATTCCACATGTACGc                                                               >  1:3673871/1‑149 (MQ=255)
                                                                                  catacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCaa                                                             >  1:3259126/1‑149 (MQ=255)
                                                                                    tacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAAtt                                                           >  1:1465082/1‑149 (MQ=255)
                                                                                      catTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTg                                                         <  2:1366862/149‑1 (MQ=255)
                                                                                        ttGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTCAGCAATTTGcc                                                       >  2:2657804/1‑149 (MQ=255)
                                                                                        ttGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGcc                                                       <  1:1067481/149‑1 (MQ=255)
                                                                                        ttGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGcc                                                       <  2:1968556/149‑1 (MQ=255)
                                                                                           gTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGc                                                    <  1:894354/149‑1 (MQ=255)
                                                                                            ttGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCt                                                   >  2:48559/1‑149 (MQ=255)
                                                                                              gtgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTg                                                 <  1:3346623/149‑1 (MQ=255)
                                                                                               tgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTgg                                                <  2:2253701/149‑1 (MQ=255)
                                                                                                  tACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCAt                                             >  1:4004110/1‑149 (MQ=255)
                                                                                                     tCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGc                                          <  1:1188635/149‑1 (MQ=255)
                                                                                                     tCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGc                                          <  1:3581487/149‑1 (MQ=255)
                                                                                                        cTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAgg                                       >  1:261176/1‑149 (MQ=255)
                                                                                                         ttATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGc                                      >  1:1825474/1‑149 (MQ=255)
                                                                                                            tcatcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAtt                                   >  1:419276/1‑149 (MQ=255)
                                                                                                            tcatcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAtt                                   <  1:1913730/149‑1 (MQ=255)
                                                                                                            tcatcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAtt                                   <  1:2461844/149‑1 (MQ=255)
                                                                                                             catcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAttt                                  >  2:2201352/1‑149 (MQ=255)
                                                                                                              atcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAtttt                                 >  1:1931636/1‑149 (MQ=255)
                                                                                                               tcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAttttt                                <  1:3989026/149‑1 (MQ=255)
                                                                                                                 aGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTc                              <  1:824941/149‑1 (MQ=255)
                                                                                                                   tGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCa                            <  2:3598635/149‑1 (MQ=255)
                                                                                                                     aGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAgg                          >  1:2435579/1‑149 (MQ=255)
                                                                                                                         aaCAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACt                      >  1:222287/1‑149 (MQ=255)
                                                                                                                           cAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACttt                    <  1:3460721/149‑1 (MQ=255)
                                                                                                                           cAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACttt                    >  2:1680700/1‑149 (MQ=255)
                                                                                                                             gagaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCtt                                                  >  1:2724391/1‑117 (MQ=255)
                                                                                                                               gaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGaa                >  2:112576/1‑149 (MQ=255)
                                                                                                                                aaTAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAAt               <  1:1729991/149‑1 (MQ=255)
                                                                                                                                 aTAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATg              <  1:3708573/149‑1 (MQ=255)
                                                                                                                                 aTAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATg              >  1:1208179/1‑149 (MQ=255)
                                                                                                                                   aGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAg            <  1:1649288/149‑1 (MQ=255)
                                                                                                                                     ttCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTa          <  2:1265769/149‑1 (MQ=255)
                                                                                                                                      tCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTAc         <  1:2891036/149‑1 (MQ=255)
                                                                                                                                        aGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTg       <  1:1745152/149‑1 (MQ=255)
                                                                                                                                          tGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGAt     <  1:3783693/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGA  >  CP009273/2778674‑2778963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑