Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,798,195 A→T 100% K8* (AAA→TAA)  proV → glycine betaine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,798,1950AT97.0% 658.3 / 7.0 201K8* (AAA→TAA) proVglycine betaine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base T (107/88);  total (111/90)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 206 total aligned reads displayed.

CAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGAT  >  CP009273/2798049‑2798340
                                                                                                                                                  |                                                                                                                                                 
cAATAGTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTtaa                                                                                                                                                 <  1:3109034/149‑1 (MQ=255)
   tatTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAAt                                                                                                                                              <  2:284547/149‑1 (MQ=255)
    atTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATc                                                                                                                                             >  1:2517701/1‑149 (MQ=255)
     ttCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCt                                                                                                                                            <  1:3936842/149‑1 (MQ=255)
        aTGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTta                                                                                                                                         <  2:3157129/149‑1 (MQ=255)
           ccACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaa                                                                                                                                      >  1:2374767/1‑149 (MQ=255)
            cacaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaaa                                                                                                                                     >  2:2380205/1‑149 (MQ=255)
            cacaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaaa                                                                                                                                     >  2:2197880/1‑149 (MQ=255)
            cacaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGGTAGGGTAGAAAAAAGTGACTATTTCCAGTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCCTGGCAATTAAATTTGAAATTTAACATCTTTATaaa                                                                                                                                     >  2:2380266/1‑149 (MQ=255)
             acaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaaaa                                                                                                                                    <  1:786101/149‑1 (MQ=255)
             acaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaaaa                                                                                                                                    <  1:1854555/149‑1 (MQ=255)
             acaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaaaa                                                                                                                                    <  2:422449/149‑1 (MQ=255)
             acaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaaaa                                                                                                                                    <  2:2357000/149‑1 (MQ=255)
               aTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAata                                                                                                                                  <  2:2960536/149‑1 (MQ=255)
               aTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAata                                                                                                                                  <  1:2247973/149‑1 (MQ=255)
               aTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAata                                                                                                                                  <  1:1209462/149‑1 (MQ=255)
                tttGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAatat                                                                                                                                 >  2:1817708/1‑149 (MQ=255)
                 ttGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATAtt                                                                                                                                <  2:81969/149‑1 (MQ=255)
                 ttGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATAtt                                                                                                                                <  1:4021174/149‑1 (MQ=255)
                 ttGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATAtt                                                                                                                                <  2:2063737/149‑1 (MQ=255)
                 ttGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATAtt                                                                                                                                <  2:3691218/149‑1 (MQ=255)
                    ccATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTgg                                                                                                                             <  2:1030129/149‑1 (MQ=255)
                    ccATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTgg                                                                                                                             <  2:2127995/149‑1 (MQ=255)
                       tCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGa                                                                                                                          >  1:3393135/1‑149 (MQ=255)
                            ggTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATc                                                                                                                     <  2:2642690/149‑1 (MQ=255)
                                  cTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACagc                                                                                                               <  2:17738/149‑1 (MQ=255)
                                  cTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACagc                                                                                                               <  1:638090/149‑1 (MQ=255)
                                    cAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACagcga                                                                                                             >  2:3939525/1‑149 (MQ=255)
                                     aGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTATAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgag                                                                                                            >  2:1198137/1‑149 (MQ=255)
                                     aGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgag                                                                                                            >  2:3250425/1‑149 (MQ=255)
                                     aGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgag                                                                                                            <  2:129571/149‑1 (MQ=255)
                                       aTTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgagcg                                                                                                          >  2:209396/1‑149 (MQ=255)
                                        ttCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGTCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGt                                                                                                         >  2:2535647/1‑149 (MQ=255)
                                         tctcAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGtt                                                                                                        <  1:423312/149‑1 (MQ=255)
                                          ctcAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTc                                                                                                       >  1:3620409/1‑149 (MQ=255)
                                          ctcAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTc                                                                                                       >  1:1576883/1‑149 (MQ=255)
                                          ctcAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTc                                                                                                       >  1:1693935/1‑149 (MQ=255)
                                               tATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAata                                                                                                  >  2:2800191/1‑149 (MQ=255)
                                                aTGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAatat                                                                                                 >  2:1092630/1‑149 (MQ=255)
                                                aTGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAatat                                                                                                 <  1:198203/149‑1 (MQ=255)
                                                  gTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAatatat                                                                                               >  1:2486074/1‑149 (MQ=255)
                                                  gTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAatatat                                                                                               <  1:3328781/149‑1 (MQ=255)
                                                     aGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGa                                                                                            <  1:670858/149‑1 (MQ=255)
                                                       ggTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAAc                                                                                          <  2:1927963/149‑1 (MQ=255)
                                                       ggTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAAc                                                                                          <  1:3146198/149‑1 (MQ=255)
                                                       ggTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAAc                                                                                          <  1:809199/149‑1 (MQ=255)
                                                        gTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACa                                                                                         >  2:1120828/1‑149 (MQ=255)
                                                          aGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAg                                                                                       <  2:3345829/149‑1 (MQ=255)
                                                           gAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAgg                                                                                      <  1:1911691/149‑1 (MQ=255)
                                                             aaaaaGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGAc                                                                                    <  1:1156692/149‑1 (MQ=255)
                                                              aaaaGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACt                                                                                   >  2:3605361/1‑149 (MQ=255)
                                                               aaaGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACtt                                                                                  >  1:2863657/1‑149 (MQ=255)
                                                                aaGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACttt                                                                                 >  1:1829414/1‑149 (MQ=255)
                                                                      cTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAg                                                                           >  2:631088/1‑149 (MQ=255)
                                                                         tttCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAAc                                                                        <  2:2855403/149‑1 (MQ=255)
                                                                         tttCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAAc                                                                        >  1:36748/1‑149 (MQ=255)
                                                                             cATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAAt                                                                    <  1:152681/149‑1 (MQ=255)
                                                                              aTTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAAtt                                                                   >  2:3314443/1‑149 (MQ=255)
                                                                                tGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCt                                                                 <  2:2157837/149‑1 (MQ=255)
                                                                                 gggTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTg                                                                <  1:688612/149‑1 (MQ=255)
                                                                                 gggTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTg                                                                <  2:4022518/149‑1 (MQ=255)
                                                                                 gggTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTg                                                                >  1:2845057/1‑149 (MQ=255)
                                                                                 gggTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTg                                                                <  1:2305677/149‑1 (MQ=255)
                                                                                    tAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaa                                                             >  1:3879268/1‑149 (MQ=255)
                                                                                    tAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaa                                                             >  1:975217/1‑149 (MQ=255)
                                                                                      atatatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGacaa                                                           >  1:1477311/1‑149 (MQ=255)
                                                                                       tatatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaaaa                                                          >  2:3658410/1‑149 (MQ=255)
                                                                                         tatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAAc                                                        >  2:1820699/1‑149 (MQ=255)
                                                                                             gACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATATTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTggg                                                    >  1:1409022/1‑149 (MQ=255)
                                                                                              aCATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACACATTCTGGAAAAAACTGGGc                                                   >  2:3994881/1‑149 (MQ=255)
                                                                                                aTAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTa                                                 >  1:1058640/1‑149 (MQ=255)
                                                                                                  aGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATc                                               <  1:2929645/149‑1 (MQ=255)
                                                                                                     cAAATAAAGTAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCt                                            <  1:2365662/149‑1 (MQ=255)
                                                                                                        aTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTgg                                         <  2:1426173/149‑1 (MQ=255)
                                                                                                         tAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGc                                        <  1:4077189/149‑1 (MQ=255)
                                                                                                          aaaGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCg                                       <  1:613203/149‑1 (MQ=255)
                                                                                                            aGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTa                                     >  1:2558472/1‑149 (MQ=255)
                                                                                                                aTCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAg                                 <  2:1693935/149‑1 (MQ=255)
                                                                                                                 tCTTTCTATTGCATGGCAATTAAATTATAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACATGGACTTTCACAATAACAAATTCTGTAAAAAACTGGTCTATCGCTTGGCGTAAAAGa                                <  1:1345277/149‑1 (MQ=255)
                                                                                                                 tCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGa                                >  2:2966035/1‑149 (MQ=255)
                                                                                                                   tttCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACg                              >  2:223906/1‑149 (MQ=255)
                                                                                                                   tttCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACg                              >  2:3369420/1‑149 (MQ=255)
                                                                                                                      cTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTCTCGCTTGGCGTAAAAGACGCCa                           >  1:2103688/1‑149 (MQ=255)
                                                                                                                       tATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAg                          >  2:697893/1‑149 (MQ=255)
                                                                                                                         ttGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTc                        <  2:665270/149‑1 (MQ=255)
                                                                                                                              tGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGcc                   >  1:1007802/1‑149 (MQ=255)
                                                                                                                               ggCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCa                  >  1:93403/1‑149 (MQ=255)
                                                                                                                                   attaaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTga              >  1:2072130/1‑149 (MQ=255)
                                                                                                                                    ttaaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaa             <  1:3351515/149‑1 (MQ=255)
                                                                                                                                    ttaaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaa             >  1:3798226/1‑149 (MQ=255)
                                                                                                                                    ttaaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaa             >  1:2386630/1‑149 (MQ=255)
                                                                                                                                     taaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaag            <  2:3816644/149‑1 (MQ=255)
                                                                                                                                       aattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaagaa          >  2:2080206/1‑149 (MQ=255)
                                                                                                                                        attaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGaagaag         >  1:1604328/1‑149 (MQ=255)
                                                                                                                                        attaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTGTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGaagaag         <  2:2884141/149‑1 (MQ=255)
                                                                                                                                            gataTTTAAAATCTTTATAAAATATTTGGCGATCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGTAAAAAACTGGGCTATCGCTTGGAGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCga     <  1:860197/146‑1 (MQ=255)
                                                                                                                                              aaTTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCgaga   >  1:2794761/1‑149 (MQ=255)
                                                                                                                                              aaTTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCgaga   >  2:3003806/1‑149 (MQ=255)
                                                                                                                                              aaTTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCgaga   >  1:734418/1‑149 (MQ=255)
                                                                                                                                               aTTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGat  >  2:3709881/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGAT  >  CP009273/2798049‑2798340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑