Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,832,598 T→G 7.6% K9T (AAG→ACG)  ascG ← asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,832,5980TG 7.6% 512.3 / 5.0 226K9T (AAG→ACG) ascGasc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base T (50/151);  new base G (22/2);  total (73/153)
Fisher's exact test for biased strand distribution p-value = 1.67e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

ACTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCA  >  CP009273/2832450‑2832746
                                                                                                                                                    |                                                                                                                                                     
aCTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCt                                                                                                                                                       <  1:3809409/149‑1 (MQ=255)
 ctctTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCtt                                                                                                                                                      >  1:3373543/1‑149 (MQ=255)
  tctTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTc                                                                                                                                                     <  2:1131462/149‑1 (MQ=255)
  tctTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTc                                                                                                                                                     <  2:3980331/149‑1 (MQ=255)
      cGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGccc                                                                                                                                                 >  2:2246452/1‑148 (MQ=255)
       gCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCAc                                                                                                                                                <  2:2921675/149‑1 (MQ=255)
        ccGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACt                                                                                                                                               <  2:3111213/149‑1 (MQ=255)
         cGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCCGCGCGCGTCGCCAcat                                                                                                                                              >  1:2471179/1‑147 (MQ=255)
         cGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACtt                                                                                                                                              <  1:2884461/149‑1 (MQ=255)
           aCAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTcc                                                                                                                                            >  2:2549213/1‑149 (MQ=255)
              gATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGc                                                                                                                                         >  2:3940373/1‑149 (MQ=255)
                ttGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAt                                                                                                                                       >  2:2310806/1‑149 (MQ=255)
                  gcgcgcCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCATCGCCACTTCCAGCAtcg                                                                                                                                     >  2:826230/1‑149 (MQ=255)
                     cgcCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAtcgtcg                                                                                                                                  <  1:1291802/149‑1 (MQ=255)
                        catcAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGtca                                                                                                                               <  2:3593090/146‑1 (MQ=255)
                          gCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCGTCGCCACTTCCAGCAACGTCGgcatc                                                                                                                             >  1:3157583/1‑149 (MQ=255)
                           cAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGtcatca                                                                                                                            <  2:3778749/149‑1 (MQ=255)
                            aaGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTcatcat                                                                                                                           <  2:2824452/149‑1 (MQ=255)
                            aaGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTcatcat                                                                                                                           <  2:1338361/149‑1 (MQ=255)
                              gTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCGTCGCCCCATCCAGCATCGTCGTCATCAatt                                                                                                                         >  1:808107/1‑149 (MQ=255)
                                ttGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTc                                                                                                                       <  2:3838719/149‑1 (MQ=255)
                                  ggACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCAt                                                                                                                     <  2:2495432/149‑1 (MQ=255)
                                     cGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATccc                                                                                                                  <  1:2219218/149‑1 (MQ=255)
                                      ggTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCGTCGCCACGTCCAGCATCGTCGGCATCAATTTAAAccca                                                                                                                 >  1:460406/1‑148 (MQ=255)
                                       gTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCtt                                                                                                                <  1:3210921/149‑1 (MQ=255)
                                        tAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCttt                                                                                                               <  1:1008148/149‑1 (MQ=255)
                                           ccGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACATCCAGCATCGTCGTCATCATTTTAATACCTTTaca                                                                                                            >  1:1965603/1‑149 (MQ=255)
                                                ctctTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTTAAACCCCCGCGCGCTTCGCCACTTCCACCAAACTCGTCATAATTTTAATACCATTaaaaccaa                                                                                                       >  1:2155284/1‑142 (MQ=255)
                                                ctctTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCaa                                                                                                       <  2:447231/149‑1 (MQ=255)
                                                ctctTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCaa                                                                                                       <  1:1470110/149‑1 (MQ=255)
                                                    tctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCaa                                                                                                   <  1:267077/149‑1 (MQ=255)
                                                     ctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAAc                                                                                                  <  1:2527554/149‑1 (MQ=255)
                                                        ccGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCa                                                                                               <  2:1095425/149‑1 (MQ=255)
                                                        ccGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCGTCGCCACTTGCAGCATCGTCGTCAACATTTTCATCCCGTTACACGCAATAAACGCa                                                                                               >  2:3786294/1‑149 (MQ=255)
                                                          gCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACATCCCGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACCCAgt                                                                                             >  2:170709/1‑149 (MQ=255)
                                                            cTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCCTCGTCGTCATCATTTTCATACCTTTACACGCAATCAACGCAgtgt                                                                                           >  2:3233564/1‑149 (MQ=255)
                                                            cTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCC‑TTACACGCAATCAACGCAGTGTa                                                                                          <  2:1560701/149‑1 (MQ=255)
                                                              gAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTAc                                                                                         <  2:653220/149‑1 (MQ=255)
                                                              gAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTAc                                                                                         <  2:2716934/149‑1 (MQ=255)
                                                               aaaCACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCAAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCCACGCAGTGTACt                                                                                        >  2:571741/1‑149 (MQ=255)
                                                                aaCACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTg                                                                                       <  1:233749/149‑1 (MQ=255)
                                                                    cgcgATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACAAGCAATCAACGCAGTTTACTGCAcc                                                                                   >  2:1288780/1‑149 (MQ=255)
                                                                    cgcgATCGTTAGTCTCCTGGCTGACGTATCCATTACCTGAAAGCACGCGGGAAAAGGTCGCTTTTTAAAAACCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCATTACACGCAATCAAAGCCGTGTACTTCAcc                                                                                   >  2:3342850/1‑149 (MQ=255)
                                                                      cgATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑Gt                                                                                <  2:1177789/149‑1 (MQ=255)
                                                                        aTCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑Gttt                                                                              >  2:3559857/1‑149 (MQ=255)
                                                                        aTCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑Gttt                                                                              <  2:111756/149‑1 (MQ=255)
                                                                        aTCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑Gttt                                                                              <  1:1771187/149‑1 (MQ=255)
                                                                        aTCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑Gttt                                                                              <  1:443293/149‑1 (MQ=255)
                                                                        aTCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑Gttt                                                                              <  1:3725388/149‑1 (MQ=255)
                                                                           tttAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGcc                                                                           <  2:2472138/149‑1 (MQ=255)
                                                                            ttAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGTAAACCCCGCGCGCGTCGCCCCCTCCCGCAGAGGCGTCATAATTTTAATCCCGTTAAACCCAAGCAACGCCCTGTACAGCACC‑GGTTGGCg                                                                          >  1:1097574/1‑149 (MQ=255)
                                                                              aGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCGGCGCGGGTGGGCACATACAGCCTCGGCGTCATAATTTTAATACCGGTTAACGCAAAAAACGCAGTGTACTGGACC‑GGTTTGCGAt                                                                        >  1:1577582/1‑149 (MQ=255)
                                                                              aGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGAAACCCCGGCGCGCTTCTCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTAAACGCAATCAACGCAGTGTACTGCACC‑GTTTTCCGAt                                                                        >  1:997147/1‑149 (MQ=255)
                                                                               gTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCCTCGTCGGCATCATTTTCATCCCGTTACACGCAATCAACGCAGTGGACTGCCCC‑CGGTGCCGAtt                                                                       >  2:190017/1‑149 (MQ=255)
                                                                                   ccTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTcc                                                                   <  2:1592518/149‑1 (MQ=255)
                                                                                    cTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCt                                                                  <  2:2332713/149‑1 (MQ=255)
                                                                                      ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCCCGTCCAGCAACGGCGGCATCATTTTAATCCCCTTACACGCCAACAACGCAGTGTACTGCACC‑GGTTTCCGATTGTCCTTg                                                                >  2:723812/1‑149 (MQ=255)
                                                                                          gACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAAAGTCGTCATAATTTTCATACCTTTACACGCAATCAAAGCAGTGTACTGCACC‑GTTTTCCGATTGTCCTTGcaca                                                            >  2:914329/1‑149 (MQ=255)
                                                                                                  cATTACCTGACAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATcggcgg                                                    <  2:2316157/149‑1 (MQ=255)
                                                                                                      aCCTGAAAGCACGCGGGAAACGGTCGCTTTTTAAACCCCGGCGCGCTTCGCCACTTCCAGAATCTTCTTCATCATTTTCATACCTTTAAACGCAATAAAATAAGTGTACTGCACC‑GTTTGCAGATTTTCCTTGCAAAATAGTCGGGaaa                                                >  2:3412301/1‑149 (MQ=255)
                                                                                                      aCCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GGTTGCCGATTGTCCTTGCACAAGCGGCGGGcaa                                                >  2:2938334/1‑149 (MQ=255)
                                                                                                          gAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGCTTGTCCTTGCACAATCGGCgggtcacata                                            >  2:2591817/1‑142 (MQ=255)
                                                                                                           aaaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAatat                                           <  1:3191700/149‑1 (MQ=255)
                                                                                                           aaaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAatat                                           <  1:649593/149‑1 (MQ=255)
                                                                                                            aaGCACGCGGTAACCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATAtt                                          <  2:1778428/149‑1 (MQ=255)
                                                                                                             aGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTc                                         <  2:3573025/149‑1 (MQ=255)
                                                                                                             aGCACGCGGGAAACGGTCGCGTTTGAAACCCCCGCGCGCGTCGCCCCGTCCAGCAACGTCGTCATCAATTTCATCCCGTTACACGCAATCAACGCCGTGTGCTGCACCGGTTTGCC‑ATTGTCCGTGCACAAACGGCGGGCAAAATATTc                                         >  1:2414263/1‑149 (MQ=255)
                                                                                                              gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                        <  1:2589802/149‑1 (MQ=255)
                                                                                                                     ggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTGCACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAcc                                 <  2:1852547/149‑1 (MQ=255)
                                                                                                                     ggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAcc                                 <  2:2065372/149‑1 (MQ=255)
                                                                                                                     ggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACGTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAcc                                 <  2:2960575/149‑1 (MQ=255)
                                                                                                                      gAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGTGAAAAATATTCAGGTGACCg                                <  1:3859807/149‑1 (MQ=255)
                                                                                                                        aaCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGt                              <  1:1189200/149‑1 (MQ=255)
                                                                                                                        aaCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGt                              <  1:505807/149‑1 (MQ=255)
                                                                                                                         aCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGtt                             <  2:411291/149‑1 (MQ=255)
                                                                                                                             tCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGACAAATATTCAGGTGACCGGTTTgac                         >  1:3955183/1‑149 (MQ=255)
                                                                                                                               gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaa                       <  1:1119583/149‑1 (MQ=255)
                                                                                                                               gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaa                       <  1:1120604/149‑1 (MQ=255)
                                                                                                                               gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaa                       >  2:12291/1‑149 (MQ=255)
                                                                                                                                cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                      <  2:589452/149‑1 (MQ=255)
                                                                                                                                cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                      <  1:1306161/149‑1 (MQ=255)
                                                                                                                                 ttttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                     >  1:2031987/1‑149 (MQ=255)
                                                                                                                                 ttttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                     <  1:1819371/149‑1 (MQ=255)
                                                                                                                                  tttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAata                    <  2:1661784/149‑1 (MQ=255)
                                                                                                                                  tttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAata                    <  2:2292014/149‑1 (MQ=255)
                                                                                                                                  tttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTGTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAata                    <  2:3863964/149‑1 (MQ=255)
                                                                                                                                   ttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCCCAATCGGCGGGACAAATATTCAGGTGACCGGTTTCACCAagat                   >  2:3613813/1‑149 (MQ=255)
                                                                                                                                   ttGAAACCCCGGCGCGCTTCCACACTTCCAGCATCTTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAatat                   <  1:3073260/149‑1 (MQ=255)
                                                                                                                                      aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaa                >  2:964710/1‑149 (MQ=255)
                                                                                                                                      aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaa                >  2:952711/1‑149 (MQ=255)
                                                                                                                                         ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaaaa             <  1:316104/149‑1 (MQ=255)
                                                                                                                                         ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaaaa             <  2:303947/149‑1 (MQ=255)
                                                                                                                                         ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaaaa             <  2:1020624/149‑1 (MQ=255)
                                                                                                                                         ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GATTGCCGAGTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaaaa             <  2:3076274/149‑1 (MQ=255)
                                                                                                                                          cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAAt            <  1:2732124/149‑1 (MQ=255)
                                                                                                                                            cGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGa          <  2:1965228/149‑1 (MQ=255)
                                                                                                                                                gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCCGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACaa      <  2:894048/149‑1 (MQ=255)
                                                                                                                                                 cgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAt     <  1:3559857/149‑1 (MQ=255)
                                                                                                                                                   cTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc   >  2:1362116/1‑149 (MQ=255)
                                                                                                                                                    ttCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCa  <  2:460406/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACC‑GTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCA  >  CP009273/2832450‑2832746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑