Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,297 T→G 11.5% S317A (TCG→GCG)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,2970TG11.5% 453.3 / 4.0 214S317A (TCG→GCG) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (70/98);  new base G (27/5);  total (111/103)
Fisher's exact test for biased strand distribution p-value = 1.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAA  >  CP009273/2835150‑2835445
                                                                                                                                                   |                                                                                                                                                    
 tgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTcg                                                                                                                                                    <  2:1415847/149‑1 (MQ=255)
 tgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCGATTACGCCTCGCGCTGCGCCTcg                                                                                                                                                    >  2:3350082/1‑149 (MQ=255)
   cAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTcggc                                                                                                                                                  <  2:1234117/149‑1 (MQ=255)
        gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgaga                                                                                                                                             <  1:495982/149‑1 (MQ=255)
         cgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGAt                                                                                                                                            <  1:1313601/149‑1 (MQ=255)
          ggggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTAAGCCACGCGCTGCGCCGCGGCGGGGATg                                                                                                                                           >  1:1679149/1‑149 (MQ=255)
            ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGaa                                                                                                                                         <  1:663676/149‑1 (MQ=255)
            ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGaa                                                                                                                                         <  1:2054178/149‑1 (MQ=255)
            ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCGCTTTCAGCTATTACGCCTAGCGCGGCGCCTCGGGGGCGATGaa                                                                                                                                         >  2:180920/1‑149 (MQ=255)
                 gTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTGTTTGAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATGTAAGccc                                                                                                                                    >  2:1765187/1‑148 (MQ=255)
                 gTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGAGGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTCTGCGATTACGCCGCGCGCTGCGCCGCGGGGGAGATGTACGccc                                                                                                                                    >  2:2105364/1‑148 (MQ=255)
                  tATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCcaa                                                                                                                                   <  1:3185795/149‑1 (MQ=255)
                   aTCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCcaac                                                                                                                                  <  2:2116973/149‑1 (MQ=255)
                   aTCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCGCGCGCGGCGCCGCGGCGGAGATGAACGCccac                                                                                                                                  >  1:3399637/1‑149 (MQ=255)
                   aTCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTCTTTGAGCGATTAAGCCTCGCGCGGCGCCTCGGCGGAGATGAAACCcaac                                                                                                                                  >  1:3990425/1‑149 (MQ=255)
                        gCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcag                                                                                                                             <  1:3899028/149‑1 (MQ=255)
                          ttACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTACGCCTCGCGCGGCGCCTCGGCGGCGATGAACGCCAACAAcagca                                                                                                                           >  2:1294192/1‑149 (MQ=255)
                           tACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                          <  1:624467/149‑1 (MQ=255)
                                tGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTATCTTTAATCTATTACGCCTCGCGCGGCGCCACGGCGGGGATGAACGCCAAAAAAAGCACTgcgg                                                                                                                     >  1:1658236/1‑149 (MQ=255)
                                tGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGGTTGTGTGTTTCAGCTAATAAGCCGCGCGCGGCGCCGCCGCGGGGTTGGACGCCCACAAAACCAGTgcgg                                                                                                                     >  1:2422696/1‑149 (MQ=255)
                                    cgcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGaa                                                                                                                 <  2:1099170/149‑1 (MQ=255)
                                     gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAAc                                                                                                                >  2:4062441/1‑149 (MQ=255)
                                       gTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTAAGCCTCGCGCGGCGCCACGGCGGAGATGAACGCCCACAACAGCAGTGCGGCGAACgg                                                                                                              >  2:496335/1‑148 (MQ=255)
                                        tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGACAAACACCGTCGATTTTGTCTCTTTCACCGATTACGCCTCGCGCAGCGCCTCCGCGGAGATGAACGCCAACAACACCAGGGCGGCGAACggg                                                                                                             >  2:3994598/1‑147 (MQ=255)
                                        tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACctg                                                                                                             >  2:2546022/1‑149 (MQ=255)
                                          ttCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTAAGCCTCGCGCTGCGCCTCGGCGGAGATTAACGCCAACAACAGCAGTGCGGCGAACgtggt                                                                                                           >  2:657416/1‑149 (MQ=255)
                                                 aaaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTGCTCTTTAAGCTATTAAGCCCCCCGCGGCGCCGCGGCCGCGAGGAACGCCCACCAAACCACTGCGGCGAACGGGGTGAAAtag                                                                                                    >  1:3245255/1‑147 (MQ=255)
                                                  aaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTACGCCTCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGAAATccc                                                                                                   >  2:3606277/1‑147 (MQ=255)
                                                  aaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTAAGCGATTAAGCCACGCGCTGCGCCTCGGCGGGGATGTAACCCAAAAACACCAGTGCGGCGAACCTGGTGAAAtacc                                                                                                   >  1:599792/1‑146 (MQ=255)
                                                  aaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTATTTAAGCTATTACGCCGCGCGCTGCGCCGCGGCGGCGATGAACGCCAACAACAACAGTGCGGCGAACGTGGGGTAATAGc                                                                                                   >  1:2585575/1‑149 (MQ=255)
                                                  aaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGc                                                                                                   >  2:2708406/1‑149 (MQ=255)
                                                   aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTCTTTAAGCTATTACCCCACCCGCGGCGCCACGGCGGAGATGAAAGCCAACAAAAGCAGTTCGGCGAACCTGGTGAAATAGCt                                                                                                  >  1:1830226/1‑149 (MQ=255)
                                                   aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAAAGCAGTGCGGCGAACGGGGTGAAATCGCt                                                                                                  >  2:1506400/1‑149 (MQ=255)
                                                    aaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCGATTACGCCTCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGAAATCGCTg                                                                                                 >  2:1021627/1‑149 (MQ=255)
                                                      ggggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCg                                                                                               >  2:673942/1‑149 (MQ=255)
                                                       gggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGt                                                                                              <  1:186357/149‑1 (MQ=255)
                                                          tAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTGTTTGAGCTATTTACCCTCGCGCGGCGCCTCGGCGGAGTTGAACGCCAACAACAGCAGTTCGGCGAACGGGGTGAAATACCGGCGTAAt                                                                                           >  1:1279103/1‑149 (MQ=255)
                                                            aCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTGAGCGAATACGCCACCCGCGGCGCCGCCGCGGAGTTGAACGCCCCCAACACCAGTGCGGCGGACGGGGGGAAAACGCTGCGGaaaac                                                                                         >  1:2548441/1‑146 (MQ=255)
                                                               aTCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTa                                                                                      <  1:2528137/149‑1 (MQ=255)
                                                                tCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTCTTTCCGCGATTACCCCGCGCCCTGCGCCGCGGCGGAGATGAACCCCCACAACCGCCGTGCGGCGGACGGGGTGTAATCGCGGCGTAATCCGTAt                                                                                     >  2:2773669/1‑149 (MQ=255)
                                                                    caaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTAc                                                                                 <  1:48053/149‑1 (MQ=255)
                                                                     aaaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACa                                                                                <  1:270206/149‑1 (MQ=255)
                                                                      aaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTAAGCCGCGCGCGGCGCCACGGCGGGGATGAACCCCAACAACAGCAGTGCGGCGCACGTGGTGAAATAGCAGCGTAATCCGTATATACAg                                                                               >  1:3306519/1‑149 (MQ=255)
                                                                      aaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAg                                                                               <  1:2280413/149‑1 (MQ=255)
                                                                      aaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAg                                                                               <  1:597904/149‑1 (MQ=255)
                                                                          aCCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                           <  2:183419/149‑1 (MQ=255)
                                                                           ccGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCGATTACGCCGCGCGCTGCGCCTCGGCGGCGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAg                                                                          >  2:2178665/1‑149 (MQ=255)
                                                                           ccGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAg                                                                          <  1:1161187/149‑1 (MQ=255)
                                                                           ccGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAg                                                                          <  2:2961266/149‑1 (MQ=255)
                                                                             gggCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCGGCGCCTCGGCGGAGATGAACGCCCACAACAGCAGTGCGGCGAACGGGGTGAAATCGCTGCGTAATCCGTATCTACCGGTGAGCg                                                                        >  2:3575205/1‑149 (MQ=255)
                                                                              ggCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGa                                                                       <  2:3679554/149‑1 (MQ=255)
                                                                                 gatgatGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCACTGCGGCGAACGTGGTGAAATAGCGGCGTAATACGTATCTAAATGTGAGCGActg                                                                    >  2:2892538/1‑149 (MQ=255)
                                                                                   tgatgaAATCCTGAAAAACACCGTCGATTTTTTCTCGTTCAGCTATTAAGCCTCGCGCTGCGCCGCGGCGGCGATGAAAGCCAACAACAGCAGTGCGGCGAACGTGGTGAAAACGCTGCGTAATCCGTATATAAAGGGGAGCGCctggg                                                                  >  2:398594/1‑149 (MQ=255)
                                                                                        aaaTCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggctgg                                                             <  1:923722/149‑1 (MQ=255)
                                                                                         aaTCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTACGCCACGCGCGGCCCCAAGGCGGAGATGAACGCCAACAACAGCCGTGCGGCGAACCTGGTGAAATCGCTGCGGAATCCCTATCTACAGGAGAGCGgctggggctggg                                                            >  2:650971/1‑149 (MQ=255)
                                                                                             cTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAAt                                                        <  2:1679149/149‑1 (MQ=255)
                                                                                              tGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAAtt                                                       <  2:1471653/149‑1 (MQ=255)
                                                                                                    acacCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCa                                                 <  2:1363364/149‑1 (MQ=255)
                                                                                                      acCGTCGTTTTTGTCTCTTTCAGCTATTCCGCCTCGCGCTGCGGCTCGGCGGAGATGAACGCCAACAACAGCTGTGGGGCTAAGGTGTTGAAATCGCTGCTTAATCGGTATCTACTGGTGAGCGACTGGGGCTGGGGAATTGATCCACt                                               <  1:832961/149‑1 (MQ=255)
                                                                                                       ccGTCGAGTTTTTGTATTTAACCAATTAAGCCGAGCCCAGCGCCACGGCCGAGATTAAAGCCCAAAACAAAAGTGCGGCCAACGTGGTGAAATCGCTGCGTAAAACGTATCAAACCGTGAGCGACTGGGGCTGGGGGATTTATACACta                                              >  2:190887/1‑148 (MQ=255)
                                                                                                           cgtttttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATGACCGCCAACAACCGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTc                                          <  1:1941291/146‑1 (MQ=255)
                                                                                                           cGATTTGGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTc                                          <  2:442843/149‑1 (MQ=255)
                                                                                                            gATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCt                                         >  1:1328200/1‑149 (MQ=255)
                                                                                                              ttttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGc                                       >  1:3239253/1‑149 (MQ=255)
                                                                                                               tttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCg                                      >  1:198060/1‑149 (MQ=255)
                                                                                                                 tGTCTCTTTCAGCTATTCCGCCTCGCGCGGCGCCGCGGCGGAGATGCACGCCAACAACAGCCGTGCGGCGAACGTGGTGAAATCGCTGCTTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTa                                    <  1:1413197/149‑1 (MQ=255)
                                                                                                                 tGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCTACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTa                                    >  1:3159101/1‑149 (MQ=255)
                                                                                                                   tctcTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATc                                  >  2:786618/1‑149 (MQ=255)
                                                                                                                   tctcTTACAGCTATAACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACCCCCGCAGTGCGGCGAACGTGGTTAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATc                                  <  1:1765187/149‑1 (MQ=255)
                                                                                                                       tttCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCa                              >  2:1880664/1‑149 (MQ=255)
                                                                                                                        ttCAGCTATTCCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCAt                             <  1:2089534/149‑1 (MQ=255)
                                                                                                                        ttCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCAt                             >  1:2712515/1‑149 (MQ=255)
                                                                                                                        ttCAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATCACCGCCAACACCAGCAGGGCGGCGACGGTGGTGAAATCGCTGCTTAACCCGTATCTACAGGTGCGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCAt                             <  1:1740845/149‑1 (MQ=255)
                                                                                                                         tCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGACTTGATCCACTCGGTCTGCGTATCACCATg                            >  2:2829231/1‑149 (MQ=255)
                                                                                                                           aGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGaa                          <  2:175993/149‑1 (MQ=255)
                                                                                                                           aGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGaa                          <  1:673942/149‑1 (MQ=255)
                                                                                                                            gCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAat                         >  1:488679/1‑149 (MQ=255)
                                                                                                                             cTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAata                        <  2:1836677/149‑1 (MQ=255)
                                                                                                                             cTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAata                        <  2:3245255/149‑1 (MQ=255)
                                                                                                                                   cGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGt                  >  1:477815/1‑149 (MQ=255)
                                                                                                                                    gCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTa                 >  2:128284/1‑149 (MQ=255)
                                                                                                                                     ccTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCCGTGCGGCGAACGTGGTTAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTAc                <  1:2472464/149‑1 (MQ=255)
                                                                                                                                     ccTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTTAAATCGCTGCTTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTAc                <  1:1062140/149‑1 (MQ=255)
                                                                                                                                     ccTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTAc                <  2:3990425/149‑1 (MQ=255)
                                                                                                                                      cTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACg               >  2:714890/1‑149 (MQ=255)
                                                                                                                                      cTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACg               <  1:3639569/149‑1 (MQ=255)
                                                                                                                                      cTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACg               >  1:1932046/1‑149 (MQ=255)
                                                                                                                                       tCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa              >  2:2664012/1‑149 (MQ=255)
                                                                                                                                        cgcgCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGAt             <  1:2372761/149‑1 (MQ=255)
                                                                                                                                         gcgcTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATc            <  2:1822019/149‑1 (MQ=255)
                                                                                                                                           gcTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGt          >  1:3623873/1‑149 (MQ=255)
                                                                                                                                           gcTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGt          >  1:2216303/1‑149 (MQ=255)
                                                                                                                                            cTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGtt         <  2:1451322/149‑1 (MQ=255)
                                                                                                                                            cTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGtt         >  2:816320/1‑149 (MQ=255)
                                                                                                                                            cTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGtt         >  2:3070674/1‑149 (MQ=255)
                                                                                                                                              gcgcCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTAt       >  1:3073594/1‑149 (MQ=255)
                                                                                                                                              gcgcCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTAt       >  1:4106962/1‑149 (MQ=255)
                                                                                                                                                gcCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCa     <  2:2969749/149‑1 (MQ=255)
                                                                                                                                                gcCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCa     <  1:3386849/149‑1 (MQ=255)
                                                                                                                                                   tCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGaa  >  1:3187061/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAA  >  CP009273/2835150‑2835445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑