Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,846,112 C→A 100% I206I (ATC→ATA)  hypD → hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,846,1120CA97.9% 892.9 / 7.3 282I206I (ATC→ATA) hypDhydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (123/153);  total (128/154)
Fisher's exact test for biased strand distribution p-value = 9.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 284 total aligned reads displayed.

CTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATC  >  CP009273/2845966‑2846257
                                                                                                                                                  |                                                                                                                                                 
ctGCAACAGGCGATAGCTCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCTGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAgg                                                                                                                                                 <  2:1921572/149‑1 (MQ=255)
ctGCAACAGGCGAAAGCGCGTGATGTACAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAgg                                                                                                                                                 <  1:1174381/149‑1 (MQ=255)
 tGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGc                                                                                                                                                <  1:2576336/149‑1 (MQ=255)
   cAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCAc                                                                                                                                              <  1:3812205/149‑1 (MQ=255)
      cAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGa                                                                                                                                           <  1:972920/149‑1 (MQ=255)
        ggCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACg                                                                                                                                         >  1:3306068/1‑149 (MQ=255)
          cGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGcc                                                                                                                                       <  1:1334562/149‑1 (MQ=255)
          cGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGcc                                                                                                                                       <  1:666133/149‑1 (MQ=255)
           gAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCt                                                                                                                                      >  1:1437112/1‑149 (MQ=255)
            aaaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCta                                                                                                                                     <  1:2951003/149‑1 (MQ=255)
              aGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCtata                                                                                                                                   <  2:3187409/149‑1 (MQ=255)
              aGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTCGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCtata                                                                                                                                   <  2:2586828/149‑1 (MQ=255)
               gcgcGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATaa                                                                                                                                  <  2:3970291/149‑1 (MQ=255)
                  cgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAttt                                                                                                                               <  2:1709289/149‑1 (MQ=255)
                   gTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtttt                                                                                                                              >  1:1929605/1‑149 (MQ=255)
                     gATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTAt                                                                                                                            <  2:1653376/149‑1 (MQ=255)
                      aTGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATc                                                                                                                           <  2:594393/149‑1 (MQ=255)
                         tgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGcc                                                                                                                        <  2:4110537/149‑1 (MQ=255)
                          gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCa                                                                                                                       <  1:971402/149‑1 (MQ=255)
                          gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCa                                                                                                                       <  2:292620/149‑1 (MQ=255)
                          gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCa                                                                                                                       <  1:2981073/149‑1 (MQ=255)
                          gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCa                                                                                                                       >  1:562394/1‑149 (MQ=255)
                            aGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGc                                                                                                                     >  2:3187905/1‑149 (MQ=255)
                             gAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCg                                                                                                                    >  1:3520632/1‑149 (MQ=255)
                                  tttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtttt                                                                                                               <  1:2307554/149‑1 (MQ=255)
                                   ttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                              <  2:2655063/149‑1 (MQ=255)
                                     aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                            <  1:742512/149‑1 (MQ=255)
                                     aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                            >  1:3634115/1‑149 (MQ=255)
                                       ttcttcTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCg                                                                                                          >  2:280893/1‑149 (MQ=255)
                                       ttcttcTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCg                                                                                                          >  2:997524/1‑149 (MQ=255)
                                          ttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTcc                                                                                                       <  2:1704066/149‑1 (MQ=255)
                                          ttctGCCAACACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTCTGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTcc                                                                                                       >  2:1406222/1‑149 (MQ=255)
                                             tGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCt                                                                                                    <  2:2271623/149‑1 (MQ=255)
                                              gCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtg                                                                                                   >  2:3013303/1‑149 (MQ=255)
                                              gCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCGATAATTTTATCGCCAGCGATTTTCATCGTCCGCtg                                                                                                   >  2:3511897/1‑149 (MQ=255)
                                               ccAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtgg                                                                                                  <  2:1316625/149‑1 (MQ=255)
                                                cAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggt                                                                                                 >  1:1005911/1‑149 (MQ=255)
                                                 aGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtg                                                                                                >  1:3682665/1‑149 (MQ=255)
                                                  gCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtgg                                                                                               >  2:1694361/1‑149 (MQ=255)
                                                   cacaTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTggtggt                                                                                              <  1:3892043/149‑1 (MQ=255)
                                                      aTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGc                                                                                           >  1:3100647/1‑149 (MQ=255)
                                                      aTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGc                                                                                           >  1:2924125/1‑149 (MQ=255)
                                                       ttACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCt                                                                                          >  2:601884/1‑149 (MQ=255)
                                                       ttACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCt                                                                                          <  2:4053924/149‑1 (MQ=255)
                                                         aCGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTgg                                                                                        <  2:1161291/149‑1 (MQ=255)
                                                         aCGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTgg                                                                                        >  2:1944368/1‑149 (MQ=255)
                                                         aCGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTgg                                                                                        >  2:2819971/1‑149 (MQ=255)
                                                            cTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAtt                                                                                     >  2:3898572/1‑149 (MQ=255)
                                                              tATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCg                                                                                   >  1:910110/1‑149 (MQ=255)
                                                              tATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCg                                                                                   >  1:1860872/1‑149 (MQ=255)
                                                                tCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGaa                                                                                 >  1:164155/1‑149 (MQ=255)
                                                                tCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGaa                                                                                 >  1:2183240/1‑149 (MQ=255)
                                                                 cccGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAc                                                                                <  1:85544/149‑1 (MQ=255)
                                                                    gACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcccc                                                                             >  1:357593/1‑149 (MQ=255)
                                                                       gTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTg                                                                          >  2:3086242/1‑149 (MQ=255)
                                                                       gTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTg                                                                          >  1:1276248/1‑149 (MQ=255)
                                                                       gTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTg                                                                          >  1:1276450/1‑149 (MQ=255)
                                                                         tGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGAt                                                                        <  1:277067/149‑1 (MQ=255)
                                                                             cAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctac                                                                    <  2:2520617/149‑1 (MQ=255)
                                                                              aGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctact                                                                   <  2:665242/149‑1 (MQ=255)
                                                                              aGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctact                                                                   >  2:3824659/1‑149 (MQ=255)
                                                                               gTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctacta                                                                  <  2:3324360/149‑1 (MQ=255)
                                                                                  tGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACaa                                                               <  2:3945705/149‑1 (MQ=255)
                                                                                   gCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAg                                                              <  2:2096386/149‑1 (MQ=255)
                                                                                    cTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAgg                                                             >  1:411448/1‑149 (MQ=255)
                                                                                     tGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGc                                                            >  1:444799/1‑149 (MQ=255)
                                                                                      ggAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCg                                                           >  2:963195/1‑149 (MQ=255)
                                                                                       gAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGt                                                          <  1:3127844/149‑1 (MQ=255)
                                                                                       gAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGt                                                          <  1:2469938/149‑1 (MQ=255)
                                                                                           aGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTc                                                      <  2:1863920/149‑1 (MQ=255)
                                                                                           aGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTc                                                      >  1:4099269/1‑149 (MQ=255)
                                                                                            gCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCa                                                     <  1:3898925/149‑1 (MQ=255)
                                                                                             ccGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCAt                                                    <  2:1467465/149‑1 (MQ=255)
                                                                                             ccGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCAt                                                    <  2:1468206/149‑1 (MQ=255)
                                                                                               ggATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACCAGGCGTGGTCATGc                                                  <  2:90257/149‑1 (MQ=255)
                                                                                               ggATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGc                                                  <  2:2169076/149‑1 (MQ=255)
                                                                                                 aTAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTg                                                <  1:1606413/149‑1 (MQ=255)
                                                                                                 aTAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTg                                                <  1:3651583/149‑1 (MQ=255)
                                                                                                     cGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgc                                            <  1:2896874/149‑1 (MQ=255)
                                                                                                       gTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcag                                          >  2:2314621/1‑149 (MQ=255)
                                                                                                              tGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaaa                                   <  1:3998479/149‑1 (MQ=255)
                                                                                                              tGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaaa                                   >  2:1936592/1‑149 (MQ=255)
                                                                                                               gCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAAt                                  >  1:541776/1‑149 (MQ=255)
                                                                                                               gCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCACCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAAt                                  <  1:2423178/149‑1 (MQ=255)
                                                                                                                cGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCATATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATa                                 <  1:3479078/149‑1 (MQ=255)
                                                                                                                 gTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAg                                >  1:20162/1‑149 (MQ=255)
                                                                                                                  ttCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGc                               >  2:2784304/1‑149 (MQ=255)
                                                                                                                  ttCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGc                               <  2:3676610/149‑1 (MQ=255)
                                                                                                                  ttCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGc                               >  2:3029321/1‑149 (MQ=255)
                                                                                                                    ccTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCgg                             <  1:3220431/149‑1 (MQ=255)
                                                                                                                      tCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGcc                           >  1:1467766/1‑149 (MQ=255)
                                                                                                                       cgcgCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCATATAATTTTATCGCCAGCTATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCATTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGccc                          <  1:2271041/149‑1 (MQ=255)
                                                                                                                        gcgcCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCca                         <  2:3160882/149‑1 (MQ=255)
                                                                                                                         cgcCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCcac                        <  2:1309076/149‑1 (MQ=255)
                                                                                                                              ggTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCaa                   <  2:3441960/149‑1 (MQ=255)
                                                                                                                                       aGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGaa          >  1:4073549/1‑149 (MQ=255)
                                                                                                                                        gTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAAt         >  1:716144/1‑149 (MQ=255)
                                                                                                                                         tATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATc        >  1:1043930/1‑149 (MQ=255)
                                                                                                                                          aTGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGGGATTTTCATCGGCCGCTGGTGGTTGCTGGATTCGACCCCCTTGATCTACTACACGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCa       <  1:1758481/149‑1 (MQ=255)
                                                                                                                                          aTGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCa       <  2:3634115/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATC  >  CP009273/2845966‑2846257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑