Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,848,002 T→A 100% C102* (TGT→TGA)  fhlA → formate hydrogenlyase transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,848,0020TA98.3% 789.2 / 1.9 239C102* (TGT→TGA) fhlAformate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (112/123);  total (115/124)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

GCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCA  >  CP009273/2847856‑2848147
                                                                                                                                                  |                                                                                                                                                 
  gcAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGaaa                                                                                                                                               >  1:2636672/1‑149 (MQ=255)
   cAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAc                                                                                                                                              >  2:2344428/1‑149 (MQ=255)
   cAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAAc                                                                                                                                              >  2:3015144/1‑149 (MQ=255)
     gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCt                                                                                                                                            >  2:1897441/1‑149 (MQ=255)
     gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCt                                                                                                                                            <  2:701577/149‑1 (MQ=255)
     gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCt                                                                                                                                            <  2:392264/149‑1 (MQ=255)
       cTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTgg                                                                                                                                          <  1:4102510/149‑1 (MQ=255)
              tGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGc                                                                                                                                   >  2:857597/1‑149 (MQ=255)
              tGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGc                                                                                                                                   >  2:2916829/1‑149 (MQ=255)
                     tattaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGc                                                                                                                            <  1:1637307/149‑1 (MQ=255)
                      attaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCa                                                                                                                           <  1:3384359/149‑1 (MQ=255)
                         aCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGt                                                                                                                        <  2:3873670/149‑1 (MQ=255)
                           gcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTgg                                                                                                                      >  1:823485/1‑149 (MQ=255)
                           gcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTgg                                                                                                                      >  1:2589747/1‑149 (MQ=255)
                             gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGc                                                                                                                    <  1:770290/149‑1 (MQ=255)
                                 cgtGAAAAAGACACCCCCATTAAATATGAAGACGACACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCtata                                                                                                                <  1:465493/149‑1 (MQ=255)
                                  gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                               <  1:1734447/149‑1 (MQ=255)
                                  gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                               <  1:489039/149‑1 (MQ=255)
                                  gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                               >  2:188779/1‑149 (MQ=255)
                                  gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTACGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                                                                                               >  2:188676/1‑149 (MQ=255)
                                   tGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAcc                                                                                                              <  1:2625202/149‑1 (MQ=255)
                                     aaaaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGAATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAccaa                                                                                                            >  2:4020849/1‑147 (MQ=255)
                                      aaaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaa                                                                                                           >  1:3580472/1‑149 (MQ=255)
                                        aaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaaaa                                                                                                         >  2:3239411/1‑149 (MQ=255)
                                         aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                                                                                        <  1:2510430/149‑1 (MQ=255)
                                         aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                                                                                        <  2:2386154/149‑1 (MQ=255)
                                            cacCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTg                                                                                                     >  1:3213075/1‑149 (MQ=255)
                                             acCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                                                                                                    <  1:3978016/149‑1 (MQ=255)
                                              cccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGt                                                                                                   >  2:1121337/1‑149 (MQ=255)
                                              cccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGt                                                                                                   >  1:1991080/1‑149 (MQ=255)
                                                cccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCa                                                                                                 >  1:368960/1‑149 (MQ=255)
                                                 ccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAc                                                                                                >  1:2398826/1‑149 (MQ=255)
                                                 ccATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCAc                                                                                                >  1:400128/1‑149 (MQ=255)
                                                  cATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACt                                                                                               >  2:1137781/1‑149 (MQ=255)
                                                    ttAAATATGAAGACGACACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                                                                                             <  2:3250174/149‑1 (MQ=255)
                                                     tAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAtt                                                                                            <  2:2636784/149‑1 (MQ=255)
                                                      aaaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTg                                                                                           >  2:1455388/1‑149 (MQ=255)
                                                       aaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGc                                                                                          <  2:398443/149‑1 (MQ=255)
                                                       aaTATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGc                                                                                          <  1:249862/149‑1 (MQ=255)
                                                        atagGAAGACGAAACTGTTCGGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGcc                                                                                         <  2:3491640/149‑1 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:3723511/1‑149 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:311101/1‑149 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:3857103/1‑149 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:1936468/1‑149 (MQ=255)
                                                           tGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGa                                                                                      >  1:1677742/1‑149 (MQ=255)
                                                            gAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGAt                                                                                     >  2:593639/1‑149 (MQ=255)
                                                             aaGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATg                                                                                    >  1:23680/1‑149 (MQ=255)
                                                              acaCGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGc                                                                                   <  1:9829/147‑1 (MQ=255)
                                                              aGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGc                                                                                   <  1:45765/149‑1 (MQ=255)
                                                               gACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGcc                                                                                  <  2:1641700/149‑1 (MQ=255)
                                                                  gAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACt                                                                               <  2:2965593/149‑1 (MQ=255)
                                                                   aaaCTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTg                                                                              <  2:2701115/149‑1 (MQ=255)
                                                                    aaCTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTgg                                                                             >  1:1240933/1‑149 (MQ=255)
                                                                    aaCTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTgg                                                                             >  2:3310395/1‑149 (MQ=255)
                                                                          tCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGcggcgg                                                                       <  1:1048486/149‑1 (MQ=255)
                                                                          tCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGcggcgg                                                                       <  2:406546/149‑1 (MQ=255)
                                                                             ggCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAg                                                                    <  2:2742466/149‑1 (MQ=255)
                                                                              gCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAgg                                                                   <  2:3603547/149‑1 (MQ=255)
                                                                               cacaCGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                  <  1:2679392/149‑1 (MQ=255)
                                                                               cacaCGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                  <  1:2859148/149‑1 (MQ=255)
                                                                                 cacGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCa                                                                <  2:425265/149‑1 (MQ=255)
                                                                                   cGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCata                                                              <  1:3180583/149‑1 (MQ=255)
                                                                                    ggTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCatat                                                             >  2:3543758/1‑149 (MQ=255)
                                                                                       ccGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGTCTATACCCAAATTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAtttt                                                          <  1:1551452/149‑1 (MQ=255)
                                                                                         ggTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtg                                                        >  1:2757442/1‑149 (MQ=255)
                                                                                         ggTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtg                                                        <  1:2946607/149‑1 (MQ=255)
                                                                                           tACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggt                                                      >  2:2594377/1‑149 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                     <  1:2220796/149‑1 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                  >  2:1752138/1‑136 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAggg                                                                  <  1:1751530/136‑1 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                     <  1:726718/149‑1 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                     <  2:3997678/149‑1 (MQ=255)
                                                                                            aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtg                                                     <  2:1738501/149‑1 (MQ=255)
                                                                                             cGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTtggtgg                                                    <  1:3015144/149‑1 (MQ=255)
                                                                                               cagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCt                                                  <  2:484059/149‑1 (MQ=255)
                                                                                               cagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCt                                                  <  2:485711/149‑1 (MQ=255)
                                                                                                agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCCCTGGCGGCGGAAGGGCATATTTTTGGTGGCtg                                                 >  2:57366/1‑149 (MQ=255)
                                                                                                         tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTAt                                        <  1:2061063/149‑1 (MQ=255)
                                                                                                             gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                    <  2:2024968/149‑1 (MQ=255)
                                                                                                             gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGt                                    <  2:3814591/149‑1 (MQ=255)
                                                                                                               ccTGATACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTa                                  <  1:3775745/149‑1 (MQ=255)
                                                                                                                   aTACGCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGAc                              <  2:838897/149‑1 (MQ=255)
                                                                                                                    tACGCTGCATTGCACTTACGAACAATATTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGTTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACg                             <  1:639145/149‑1 (MQ=255)
                                                                                                                       gCTGCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATc                          <  1:3605386/149‑1 (MQ=255)
                                                                                                                          gCATTGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAATGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGcc                       >  1:1014667/1‑149 (MQ=255)
                                                                                                                             ttGCAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCt                    <  1:233103/149‑1 (MQ=255)
                                                                                                                                cAGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGa                 <  1:1457579/149‑1 (MQ=255)
                                                                                                                                 aGTTACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAg                >  2:587097/1‑149 (MQ=255)
                                                                                                                                    tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGa             >  1:773526/1‑149 (MQ=255)
                                                                                                                                    tACGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGa             >  2:1164744/1‑149 (MQ=255)
                                                                                                                                     aCGAAGAATTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaa            >  1:517927/1‑149 (MQ=255)
                                                                                                                                       gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaa          >  1:2823729/1‑149 (MQ=255)
                                                                                                                                       gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaa          >  1:1368022/1‑149 (MQ=255)
                                                                                                                                          gaaTTATGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCTAAAAaga       <  2:743698/149‑1 (MQ=255)
                                                                                                                                           aaTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAagag      >  2:206638/1‑149 (MQ=255)
                                                                                                                                            aTTTTGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGt     <  2:2531602/149‑1 (MQ=255)
                                                                                                                                             ttttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGTATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGtt    <  1:2151498/149‑1 (MQ=255)
                                                                                                                                             ttttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGtt    >  2:3211270/1‑149 (MQ=255)
                                                                                                                                               ttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCa  <  1:735844/149‑1 (MQ=255)
                                                                                                                                               ttGAGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCa  >  2:1064605/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCA  >  CP009273/2847856‑2848147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑